rabbit anti gaba  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs rabbit anti gaba
    Diffusion properties of GABAAR <t> γ2 </t> and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.
    Rabbit Anti Gaba, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gaba/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gaba - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Diffusion Barriers Constrain Receptors at Synapses"

    Article Title: Diffusion Barriers Constrain Receptors at Synapses

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0043032

    Diffusion properties of GABAAR  γ2  and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.
    Figure Legend Snippet: Diffusion properties of GABAAR γ2 and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.

    Techniques Used: Diffusion-based Assay

    (A) Examples of GABA A R γ2 trajectories at inhibitory synapses for passing (top) and trapped (bottom) receptors outside (black) or inside (grey) synapses. Scale, 0.5 µm. (B) Proportion (mean ± SEM) of γ2 (black) and α5 (white) trajectories classified as trapped at inhibitory synapses (t-test, **p<10 −2 ). (C) Trapped (trap) γ2 (black) and α5 (white) have lower mobility (median D ±25%–75% IQR) in inhibitory synapses than passing ones (KS test, ***p<10 −3 ). (D) Passing (pass) γ2 (black) and α5 (white) explored a similar surface area (median EA ±25%–75% IQR) while trapped γ2 explored a smaller surface area than α5 (KS test, *p<5×10 −2 ; ***p<10 −3 ).
    Figure Legend Snippet: (A) Examples of GABA A R γ2 trajectories at inhibitory synapses for passing (top) and trapped (bottom) receptors outside (black) or inside (grey) synapses. Scale, 0.5 µm. (B) Proportion (mean ± SEM) of γ2 (black) and α5 (white) trajectories classified as trapped at inhibitory synapses (t-test, **p<10 −2 ). (C) Trapped (trap) γ2 (black) and α5 (white) have lower mobility (median D ±25%–75% IQR) in inhibitory synapses than passing ones (KS test, ***p<10 −3 ). (D) Passing (pass) γ2 (black) and α5 (white) explored a similar surface area (median EA ±25%–75% IQR) while trapped γ2 explored a smaller surface area than α5 (KS test, *p<5×10 −2 ; ***p<10 −3 ).

    Techniques Used:

    (A) Dendritic portion of a neuron co-transfected with SEP-GABA A Rγ2 (green) and DsRed-homer1c (red) constructs. Scale, 1 µm. Note that SEP-GABA A R γ2 (green) form clusters that are not colocalized with DsRed-homer1c (red) fluorescent clusters. (B) Normalized SEP-GABA A Rγ2 FRAP fluorescence recovery curves at inhibitory synapses (green, inh), excitatory synapses (red, exc), and in extrasynaptic compartment (black, ex). (C) Percentage (mean ± SEM) of SEP-γ2 fluorescence recovery at inhibitory synapses (inh, green), excitatory synapses (exc, red) and extrasynaptically (ex, black). Note the increase in the size of the pool of slowly mobile γ2 at excitatory synapses as compared with the extrasynaptic compartment (t-test, ***p<10 −3 ). (D–E) Time constants (mean ± SEM) for the fast (D) and slow (E) pool, obtained from the double exponential fit (see Materials and Methods) applied to the data in B for inhibitory (green, inh), excitatory (red, exc) synapses or extrasynaptic area (black, ex) (t test, ns: not significant, *: p<0.05, **: p<0.01; ***: p<0.001).
    Figure Legend Snippet: (A) Dendritic portion of a neuron co-transfected with SEP-GABA A Rγ2 (green) and DsRed-homer1c (red) constructs. Scale, 1 µm. Note that SEP-GABA A R γ2 (green) form clusters that are not colocalized with DsRed-homer1c (red) fluorescent clusters. (B) Normalized SEP-GABA A Rγ2 FRAP fluorescence recovery curves at inhibitory synapses (green, inh), excitatory synapses (red, exc), and in extrasynaptic compartment (black, ex). (C) Percentage (mean ± SEM) of SEP-γ2 fluorescence recovery at inhibitory synapses (inh, green), excitatory synapses (exc, red) and extrasynaptically (ex, black). Note the increase in the size of the pool of slowly mobile γ2 at excitatory synapses as compared with the extrasynaptic compartment (t-test, ***p<10 −3 ). (D–E) Time constants (mean ± SEM) for the fast (D) and slow (E) pool, obtained from the double exponential fit (see Materials and Methods) applied to the data in B for inhibitory (green, inh), excitatory (red, exc) synapses or extrasynaptic area (black, ex) (t test, ns: not significant, *: p<0.05, **: p<0.01; ***: p<0.001).

    Techniques Used: Transfection, Construct, Fluorescence

    (A) Hippocampal cultured neurons from mRFP-gephyrin knock-in mice transfected with GFP, venus-G(2), or venus-E constructs, and stained for γ2. Scale, 2 µm. Note that mRFP-gephyrin and GABA A R γ2 formed numerous clusters along dendrites of GFP transfected neurons. Many mRFP-gephyrin clusters were colocalized with GABA A R γ2 clusters (arrows). Over-expression of the venus-G(2) and venus-E chimera resulted in a marked reduction of the number and intensity of mRFP-gephyrin and GABA A R γ2 clusters. The remaining mRFP-gephyrin clusters were rarely colocalized with γ2 clusters (arrows). (B-E) Quantifications showing venus-G(2) and venus-E constructs reduced both the number of gephyrin (B) and γ2 (D) clusters per 10 µm dendritic length and the fluorescence intensity of the corresponding clusters (C, E). Values are mean ± SEM. t-test, ns: not significant, **: p<0.01; ***: p<10 −3 .
    Figure Legend Snippet: (A) Hippocampal cultured neurons from mRFP-gephyrin knock-in mice transfected with GFP, venus-G(2), or venus-E constructs, and stained for γ2. Scale, 2 µm. Note that mRFP-gephyrin and GABA A R γ2 formed numerous clusters along dendrites of GFP transfected neurons. Many mRFP-gephyrin clusters were colocalized with GABA A R γ2 clusters (arrows). Over-expression of the venus-G(2) and venus-E chimera resulted in a marked reduction of the number and intensity of mRFP-gephyrin and GABA A R γ2 clusters. The remaining mRFP-gephyrin clusters were rarely colocalized with γ2 clusters (arrows). (B-E) Quantifications showing venus-G(2) and venus-E constructs reduced both the number of gephyrin (B) and γ2 (D) clusters per 10 µm dendritic length and the fluorescence intensity of the corresponding clusters (C, E). Values are mean ± SEM. t-test, ns: not significant, **: p<0.01; ***: p<10 −3 .

    Techniques Used: Cell Culture, Knock-In, Transfection, Construct, Staining, Over Expression, Fluorescence

    rabbit anti gaba  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs rabbit anti gaba
    Diffusion properties of GABAAR <t> γ2 </t> and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.
    Rabbit Anti Gaba, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gaba/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gaba - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Diffusion Barriers Constrain Receptors at Synapses"

    Article Title: Diffusion Barriers Constrain Receptors at Synapses

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0043032

    Diffusion properties of GABAAR  γ2  and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.
    Figure Legend Snippet: Diffusion properties of GABAAR γ2 and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.

    Techniques Used: Diffusion-based Assay

    (A) Examples of GABA A R γ2 trajectories at inhibitory synapses for passing (top) and trapped (bottom) receptors outside (black) or inside (grey) synapses. Scale, 0.5 µm. (B) Proportion (mean ± SEM) of γ2 (black) and α5 (white) trajectories classified as trapped at inhibitory synapses (t-test, **p<10 −2 ). (C) Trapped (trap) γ2 (black) and α5 (white) have lower mobility (median D ±25%–75% IQR) in inhibitory synapses than passing ones (KS test, ***p<10 −3 ). (D) Passing (pass) γ2 (black) and α5 (white) explored a similar surface area (median EA ±25%–75% IQR) while trapped γ2 explored a smaller surface area than α5 (KS test, *p<5×10 −2 ; ***p<10 −3 ).
    Figure Legend Snippet: (A) Examples of GABA A R γ2 trajectories at inhibitory synapses for passing (top) and trapped (bottom) receptors outside (black) or inside (grey) synapses. Scale, 0.5 µm. (B) Proportion (mean ± SEM) of γ2 (black) and α5 (white) trajectories classified as trapped at inhibitory synapses (t-test, **p<10 −2 ). (C) Trapped (trap) γ2 (black) and α5 (white) have lower mobility (median D ±25%–75% IQR) in inhibitory synapses than passing ones (KS test, ***p<10 −3 ). (D) Passing (pass) γ2 (black) and α5 (white) explored a similar surface area (median EA ±25%–75% IQR) while trapped γ2 explored a smaller surface area than α5 (KS test, *p<5×10 −2 ; ***p<10 −3 ).

    Techniques Used:

    (A) Dendritic portion of a neuron co-transfected with SEP-GABA A Rγ2 (green) and DsRed-homer1c (red) constructs. Scale, 1 µm. Note that SEP-GABA A R γ2 (green) form clusters that are not colocalized with DsRed-homer1c (red) fluorescent clusters. (B) Normalized SEP-GABA A Rγ2 FRAP fluorescence recovery curves at inhibitory synapses (green, inh), excitatory synapses (red, exc), and in extrasynaptic compartment (black, ex). (C) Percentage (mean ± SEM) of SEP-γ2 fluorescence recovery at inhibitory synapses (inh, green), excitatory synapses (exc, red) and extrasynaptically (ex, black). Note the increase in the size of the pool of slowly mobile γ2 at excitatory synapses as compared with the extrasynaptic compartment (t-test, ***p<10 −3 ). (D–E) Time constants (mean ± SEM) for the fast (D) and slow (E) pool, obtained from the double exponential fit (see Materials and Methods) applied to the data in B for inhibitory (green, inh), excitatory (red, exc) synapses or extrasynaptic area (black, ex) (t test, ns: not significant, *: p<0.05, **: p<0.01; ***: p<0.001).
    Figure Legend Snippet: (A) Dendritic portion of a neuron co-transfected with SEP-GABA A Rγ2 (green) and DsRed-homer1c (red) constructs. Scale, 1 µm. Note that SEP-GABA A R γ2 (green) form clusters that are not colocalized with DsRed-homer1c (red) fluorescent clusters. (B) Normalized SEP-GABA A Rγ2 FRAP fluorescence recovery curves at inhibitory synapses (green, inh), excitatory synapses (red, exc), and in extrasynaptic compartment (black, ex). (C) Percentage (mean ± SEM) of SEP-γ2 fluorescence recovery at inhibitory synapses (inh, green), excitatory synapses (exc, red) and extrasynaptically (ex, black). Note the increase in the size of the pool of slowly mobile γ2 at excitatory synapses as compared with the extrasynaptic compartment (t-test, ***p<10 −3 ). (D–E) Time constants (mean ± SEM) for the fast (D) and slow (E) pool, obtained from the double exponential fit (see Materials and Methods) applied to the data in B for inhibitory (green, inh), excitatory (red, exc) synapses or extrasynaptic area (black, ex) (t test, ns: not significant, *: p<0.05, **: p<0.01; ***: p<0.001).

    Techniques Used: Transfection, Construct, Fluorescence

    (A) Hippocampal cultured neurons from mRFP-gephyrin knock-in mice transfected with GFP, venus-G(2), or venus-E constructs, and stained for γ2. Scale, 2 µm. Note that mRFP-gephyrin and GABA A R γ2 formed numerous clusters along dendrites of GFP transfected neurons. Many mRFP-gephyrin clusters were colocalized with GABA A R γ2 clusters (arrows). Over-expression of the venus-G(2) and venus-E chimera resulted in a marked reduction of the number and intensity of mRFP-gephyrin and GABA A R γ2 clusters. The remaining mRFP-gephyrin clusters were rarely colocalized with γ2 clusters (arrows). (B-E) Quantifications showing venus-G(2) and venus-E constructs reduced both the number of gephyrin (B) and γ2 (D) clusters per 10 µm dendritic length and the fluorescence intensity of the corresponding clusters (C, E). Values are mean ± SEM. t-test, ns: not significant, **: p<0.01; ***: p<10 −3 .
    Figure Legend Snippet: (A) Hippocampal cultured neurons from mRFP-gephyrin knock-in mice transfected with GFP, venus-G(2), or venus-E constructs, and stained for γ2. Scale, 2 µm. Note that mRFP-gephyrin and GABA A R γ2 formed numerous clusters along dendrites of GFP transfected neurons. Many mRFP-gephyrin clusters were colocalized with GABA A R γ2 clusters (arrows). Over-expression of the venus-G(2) and venus-E chimera resulted in a marked reduction of the number and intensity of mRFP-gephyrin and GABA A R γ2 clusters. The remaining mRFP-gephyrin clusters were rarely colocalized with γ2 clusters (arrows). (B-E) Quantifications showing venus-G(2) and venus-E constructs reduced both the number of gephyrin (B) and γ2 (D) clusters per 10 µm dendritic length and the fluorescence intensity of the corresponding clusters (C, E). Values are mean ± SEM. t-test, ns: not significant, **: p<0.01; ***: p<10 −3 .

    Techniques Used: Cell Culture, Knock-In, Transfection, Construct, Staining, Over Expression, Fluorescence

    igg  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs igg
    Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg - by Bioz Stars, 2023-01
    94/100 stars

    Images

    aga 015  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs aga 015
    Aga 015, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aga 015/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aga 015 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    anti gabaaγ2  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs anti gabaaγ2
    Anti Gabaaγ2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gabaaγ2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gabaaγ2 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    gaba  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs gaba
    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, <t>and</t> <t>MAP2.</t> Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of <t>GABA</t> A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Gaba, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gaba/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gaba - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development"

    Article Title: The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201209132

    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Figure Legend Snippet: IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.

    Techniques Used: Binding Assay, Cell Culture, Expressing, Labeling, Immunofluorescence, Staining

    Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.
    Figure Legend Snippet: Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.

    Techniques Used: Immunofluorescence, Imaging, Cell Culture, Staining, Double Staining, Light Microscopy

    gaba  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs gaba
    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, <t>and</t> <t>MAP2.</t> Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of <t>GABA</t> A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Gaba, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gaba/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gaba - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development"

    Article Title: The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201209132

    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Figure Legend Snippet: IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.

    Techniques Used: Binding Assay, Cell Culture, Expressing, Labeling, Immunofluorescence, Staining

    Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.
    Figure Legend Snippet: Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.

    Techniques Used: Immunofluorescence, Imaging, Cell Culture, Staining, Double Staining, Light Microscopy

    gabaa subunit n termini  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs gabaa subunit n termini
    Gabaa Subunit N Termini, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gabaa subunit n termini/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gabaa subunit n termini - by Bioz Stars, 2023-01
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs rabbit anti gaba
    Diffusion properties of GABAAR <t> γ2 </t> and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.
    Rabbit Anti Gaba, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gaba/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gaba - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs igg
    Diffusion properties of GABAAR <t> γ2 </t> and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.
    Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs aga 015
    Diffusion properties of GABAAR <t> γ2 </t> and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.
    Aga 015, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aga 015/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aga 015 - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs anti gabaaγ2
    Diffusion properties of GABAAR <t> γ2 </t> and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.
    Anti Gabaaγ2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gabaaγ2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gabaaγ2 - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs gaba
    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, <t>and</t> <t>MAP2.</t> Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of <t>GABA</t> A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Gaba, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gaba/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gaba - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs gabaa subunit n termini
    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, <t>and</t> <t>MAP2.</t> Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of <t>GABA</t> A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Gabaa Subunit N Termini, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gabaa subunit n termini/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gabaa subunit n termini - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    Image Search Results


    Diffusion properties of GABAAR  γ2  and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.

    Journal: PLoS ONE

    Article Title: Diffusion Barriers Constrain Receptors at Synapses

    doi: 10.1371/journal.pone.0043032

    Figure Lengend Snippet: Diffusion properties of GABAAR γ2 and α5 subunits, and of AMPAR GluA2 subunit in relation with excitatory and inhibitory synapses.

    Article Snippet: Fritschy), rabbit anti- GABA A R γ2 subunit (1∶100; Alomone Labs, Jerusalem, Israel), mouse anti-gephyrin (mAb7a, 1.25 µg/ml; Synaptic Systems, Gottingen, Germany) and mouse anti-GluA2 (1∶200; BD Pharmingen, Franklin Lakes, USA).

    Techniques: Diffusion-based Assay

    (A) Examples of GABA A R γ2 trajectories at inhibitory synapses for passing (top) and trapped (bottom) receptors outside (black) or inside (grey) synapses. Scale, 0.5 µm. (B) Proportion (mean ± SEM) of γ2 (black) and α5 (white) trajectories classified as trapped at inhibitory synapses (t-test, **p<10 −2 ). (C) Trapped (trap) γ2 (black) and α5 (white) have lower mobility (median D ±25%–75% IQR) in inhibitory synapses than passing ones (KS test, ***p<10 −3 ). (D) Passing (pass) γ2 (black) and α5 (white) explored a similar surface area (median EA ±25%–75% IQR) while trapped γ2 explored a smaller surface area than α5 (KS test, *p<5×10 −2 ; ***p<10 −3 ).

    Journal: PLoS ONE

    Article Title: Diffusion Barriers Constrain Receptors at Synapses

    doi: 10.1371/journal.pone.0043032

    Figure Lengend Snippet: (A) Examples of GABA A R γ2 trajectories at inhibitory synapses for passing (top) and trapped (bottom) receptors outside (black) or inside (grey) synapses. Scale, 0.5 µm. (B) Proportion (mean ± SEM) of γ2 (black) and α5 (white) trajectories classified as trapped at inhibitory synapses (t-test, **p<10 −2 ). (C) Trapped (trap) γ2 (black) and α5 (white) have lower mobility (median D ±25%–75% IQR) in inhibitory synapses than passing ones (KS test, ***p<10 −3 ). (D) Passing (pass) γ2 (black) and α5 (white) explored a similar surface area (median EA ±25%–75% IQR) while trapped γ2 explored a smaller surface area than α5 (KS test, *p<5×10 −2 ; ***p<10 −3 ).

    Article Snippet: Fritschy), rabbit anti- GABA A R γ2 subunit (1∶100; Alomone Labs, Jerusalem, Israel), mouse anti-gephyrin (mAb7a, 1.25 µg/ml; Synaptic Systems, Gottingen, Germany) and mouse anti-GluA2 (1∶200; BD Pharmingen, Franklin Lakes, USA).

    Techniques:

    (A) Dendritic portion of a neuron co-transfected with SEP-GABA A Rγ2 (green) and DsRed-homer1c (red) constructs. Scale, 1 µm. Note that SEP-GABA A R γ2 (green) form clusters that are not colocalized with DsRed-homer1c (red) fluorescent clusters. (B) Normalized SEP-GABA A Rγ2 FRAP fluorescence recovery curves at inhibitory synapses (green, inh), excitatory synapses (red, exc), and in extrasynaptic compartment (black, ex). (C) Percentage (mean ± SEM) of SEP-γ2 fluorescence recovery at inhibitory synapses (inh, green), excitatory synapses (exc, red) and extrasynaptically (ex, black). Note the increase in the size of the pool of slowly mobile γ2 at excitatory synapses as compared with the extrasynaptic compartment (t-test, ***p<10 −3 ). (D–E) Time constants (mean ± SEM) for the fast (D) and slow (E) pool, obtained from the double exponential fit (see Materials and Methods) applied to the data in B for inhibitory (green, inh), excitatory (red, exc) synapses or extrasynaptic area (black, ex) (t test, ns: not significant, *: p<0.05, **: p<0.01; ***: p<0.001).

    Journal: PLoS ONE

    Article Title: Diffusion Barriers Constrain Receptors at Synapses

    doi: 10.1371/journal.pone.0043032

    Figure Lengend Snippet: (A) Dendritic portion of a neuron co-transfected with SEP-GABA A Rγ2 (green) and DsRed-homer1c (red) constructs. Scale, 1 µm. Note that SEP-GABA A R γ2 (green) form clusters that are not colocalized with DsRed-homer1c (red) fluorescent clusters. (B) Normalized SEP-GABA A Rγ2 FRAP fluorescence recovery curves at inhibitory synapses (green, inh), excitatory synapses (red, exc), and in extrasynaptic compartment (black, ex). (C) Percentage (mean ± SEM) of SEP-γ2 fluorescence recovery at inhibitory synapses (inh, green), excitatory synapses (exc, red) and extrasynaptically (ex, black). Note the increase in the size of the pool of slowly mobile γ2 at excitatory synapses as compared with the extrasynaptic compartment (t-test, ***p<10 −3 ). (D–E) Time constants (mean ± SEM) for the fast (D) and slow (E) pool, obtained from the double exponential fit (see Materials and Methods) applied to the data in B for inhibitory (green, inh), excitatory (red, exc) synapses or extrasynaptic area (black, ex) (t test, ns: not significant, *: p<0.05, **: p<0.01; ***: p<0.001).

    Article Snippet: Fritschy), rabbit anti- GABA A R γ2 subunit (1∶100; Alomone Labs, Jerusalem, Israel), mouse anti-gephyrin (mAb7a, 1.25 µg/ml; Synaptic Systems, Gottingen, Germany) and mouse anti-GluA2 (1∶200; BD Pharmingen, Franklin Lakes, USA).

    Techniques: Transfection, Construct, Fluorescence

    (A) Hippocampal cultured neurons from mRFP-gephyrin knock-in mice transfected with GFP, venus-G(2), or venus-E constructs, and stained for γ2. Scale, 2 µm. Note that mRFP-gephyrin and GABA A R γ2 formed numerous clusters along dendrites of GFP transfected neurons. Many mRFP-gephyrin clusters were colocalized with GABA A R γ2 clusters (arrows). Over-expression of the venus-G(2) and venus-E chimera resulted in a marked reduction of the number and intensity of mRFP-gephyrin and GABA A R γ2 clusters. The remaining mRFP-gephyrin clusters were rarely colocalized with γ2 clusters (arrows). (B-E) Quantifications showing venus-G(2) and venus-E constructs reduced both the number of gephyrin (B) and γ2 (D) clusters per 10 µm dendritic length and the fluorescence intensity of the corresponding clusters (C, E). Values are mean ± SEM. t-test, ns: not significant, **: p<0.01; ***: p<10 −3 .

    Journal: PLoS ONE

    Article Title: Diffusion Barriers Constrain Receptors at Synapses

    doi: 10.1371/journal.pone.0043032

    Figure Lengend Snippet: (A) Hippocampal cultured neurons from mRFP-gephyrin knock-in mice transfected with GFP, venus-G(2), or venus-E constructs, and stained for γ2. Scale, 2 µm. Note that mRFP-gephyrin and GABA A R γ2 formed numerous clusters along dendrites of GFP transfected neurons. Many mRFP-gephyrin clusters were colocalized with GABA A R γ2 clusters (arrows). Over-expression of the venus-G(2) and venus-E chimera resulted in a marked reduction of the number and intensity of mRFP-gephyrin and GABA A R γ2 clusters. The remaining mRFP-gephyrin clusters were rarely colocalized with γ2 clusters (arrows). (B-E) Quantifications showing venus-G(2) and venus-E constructs reduced both the number of gephyrin (B) and γ2 (D) clusters per 10 µm dendritic length and the fluorescence intensity of the corresponding clusters (C, E). Values are mean ± SEM. t-test, ns: not significant, **: p<0.01; ***: p<10 −3 .

    Article Snippet: Fritschy), rabbit anti- GABA A R γ2 subunit (1∶100; Alomone Labs, Jerusalem, Israel), mouse anti-gephyrin (mAb7a, 1.25 µg/ml; Synaptic Systems, Gottingen, Germany) and mouse anti-GluA2 (1∶200; BD Pharmingen, Franklin Lakes, USA).

    Techniques: Cell Culture, Knock-In, Transfection, Construct, Staining, Over Expression, Fluorescence

    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.

    Journal: The Journal of Cell Biology

    Article Title: The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development

    doi: 10.1083/jcb.201209132

    Figure Lengend Snippet: IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.

    Article Snippet: The following antibodies are commercially available: GABA A Rγ2, vGlut1, VGAT, gephyrin, neuroligin 2 (Synaptic Systems); neuroligin1, Myc, and HA (Santa Cruz Biotechnology, Inc.); IgSF9b (HPA010802; rabbit), MAP2, α-tubulin, Flag, and synaptophysin (Sigma-Aldrich); PSD-95 (Thermo Fisher Scientific); GABA A Rα1 (Alomone Labs); GAD67, synapsin I (EMD Millipore), and GAD65 (Developmental Studies Hybridoma Bank); GAD65/67 (Enzo Life Sciences); and Cy3-conjugated anti-Fc (Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Binding Assay, Cell Culture, Expressing, Labeling, Immunofluorescence, Staining

    Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.

    Journal: The Journal of Cell Biology

    Article Title: The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development

    doi: 10.1083/jcb.201209132

    Figure Lengend Snippet: Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.

    Article Snippet: The following antibodies are commercially available: GABA A Rγ2, vGlut1, VGAT, gephyrin, neuroligin 2 (Synaptic Systems); neuroligin1, Myc, and HA (Santa Cruz Biotechnology, Inc.); IgSF9b (HPA010802; rabbit), MAP2, α-tubulin, Flag, and synaptophysin (Sigma-Aldrich); PSD-95 (Thermo Fisher Scientific); GABA A Rα1 (Alomone Labs); GAD67, synapsin I (EMD Millipore), and GAD65 (Developmental Studies Hybridoma Bank); GAD65/67 (Enzo Life Sciences); and Cy3-conjugated anti-Fc (Jackson ImmunoResearch Laboratories, Inc.).

    Techniques: Immunofluorescence, Imaging, Cell Culture, Staining, Double Staining, Light Microscopy