igg  (Alomone Labs)


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    Alomone Labs igg
    Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg - by Bioz Stars, 2023-01
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    igg  (Alomone Labs)


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    Structured Review

    Alomone Labs igg
    Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg - by Bioz Stars, 2023-01
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    gaba  (Alomone Labs)


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    Structured Review

    Alomone Labs gaba
    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, <t>and</t> <t>MAP2.</t> Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of <t>GABA</t> A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Gaba, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gaba/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gaba - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development"

    Article Title: The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201209132

    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Figure Legend Snippet: IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.

    Techniques Used: Binding Assay, Cell Culture, Expressing, Labeling, Immunofluorescence, Staining

    Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.
    Figure Legend Snippet: Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.

    Techniques Used: Immunofluorescence, Imaging, Cell Culture, Staining, Double Staining, Light Microscopy

    gaba  (Alomone Labs)


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    Structured Review

    Alomone Labs gaba
    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, <t>and</t> <t>MAP2.</t> Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of <t>GABA</t> A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Gaba, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gaba/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gaba - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development"

    Article Title: The adhesion protein IgSF9b is coupled to neuroligin 2 via S-SCAM to promote inhibitory synapse development

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201209132

    IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.
    Figure Legend Snippet: IgSF9b is abundant in GABAergic interneurons and mainly present at inhibitory synapses. (A) Domain structure of IgSF9b. SP, signal peptide; Ig, immunoglobulin domain; FNIII, fibronectin type III domain; TM, transmembrane domain; PB, PDZ domain-binding motif. (B) IgSF9b signals are more abundant in interneurons than in pyramidal neurons. Cultured hippocampal neurons (DIV 28, a stage after IgSF9b reaches a plateau of its expression) were labeled by triple immunofluorescence staining for IgSF9b, GAD67, and MAP2. Yellow and red asterisks indicate cell bodies of GAD67-positive interneurons and GAD67-negative pyramidal neurons, respectively. (C and D) IgSF9b colocalizes with VGAT (an inhibitory presynaptic protein) both in pyramidal neurons (C) and in interneurons (D). The bottom images are enlarged views of the boxed regions. (E–G) IgSF9b colocalizes with gephyrin (E) and the α1 subunit of GABA A receptors (F) at VGAT- or GAD65 (an inhibitory presynaptic protein)-positive inhibitory synapses, but not with vGlut1 (an excitatory presynaptic protein)- or Shank (an excitatory postsynaptic protein)-positive excitatory synapses (G) in interneurons. (H–J) In cultured cortical neurons at DIV 28, IgSF9b is more abundantly expressed in GAD65-positive interneurons than in GAD65-negative pyramidal neurons, and colocalizes with GAD65-positive inhibitory synapses (I and J). (K and L) IgSF9b colocalizes with gephyrin and GAD65 but not with PSD-95 in dendrites of cortical interneurons. Bars: (B) 20 µm; (C and D, top rows) 20 µm; (C and D, bottom rows) 5 µm; (E–G) 10 µm; (H) 20 µm; (I–L) 5 µm.

    Techniques Used: Binding Assay, Cell Culture, Expressing, Labeling, Immunofluorescence, Staining

    Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.
    Figure Legend Snippet: Gephyrin colocalizes with neuroligin 2 and the γ2 subunit of GABA A receptors, whereas IgSF9b displays close but minimally overlapping localization with gephyrin or the γ2 subunit of GABA A receptors. (A) In normal-resolution immunofluorescence imaging, IgSF9b and gephyrin show closely associated but distinct distribution patterns at GAD65-positive inhibitory synapses. Cultured hippocampal neurons (DIV 21) were triply stained for IgSF9b, gephyrin, and GAD65. (B and C) Cultured hippocampal neurons (DIV 21) were visualized by the combinations of double staining indicated and normal (non-STORM) light microscopy. Bars: (A, left) 20 µm; (A, right) 2 µm; (B and C) 1 µm.

    Techniques Used: Immunofluorescence, Imaging, Cell Culture, Staining, Double Staining, Light Microscopy

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