γ2 subunit  (Alomone Labs)


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    Alomone Labs γ2 subunit
    MWCNT substrates increase the number of GABAergic synaptic contacts and tune PSC amplitude without affecting PSC kinetics. A , Confocal images of hippocampal neurons in culture (9 d in vitro ) in control ( a ) and CNT ( b ) identifying the presynaptic VGAT (green) and the postsynaptic <t>γ2</t> subunit of GABA A receptors (red). a , Hippocampal neurons cultured on untreated glass coverslips. Scale bar: 10 μm. a1–a3 , Higher magnification of the region highlighted by the white box in a showing the VGAT (green) and GABA A receptor γ2 subunit (red) clusters. Colocalization of VGAT and GABA A γ2 clusters identify synapses. Scale bar: 2 μm. b , Hippocampal CNT neurons. Scale bar: 10 μm. b1–b3 , Higher magnification of the region highlighted by the white box in b . Note the increased number of the VGAT (green) and GABA A receptor γ2 subunit (red) clusters and of colocalized VGAT and GABA A γ2 clusters. Scale bar: 2 μm. c , Histograms summarize results from three different culture series. CNT neurons display 1.6 times as many synapses compared to control neurons (252.0 ± 20.1 and 401.3 ± 31.5 synapses in control and CNT, respectively; n = 24 and n = 28 fields for control and CNT, respectively; ** p
    γ2 Subunit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/γ2 subunit/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    γ2 subunit - by Bioz Stars, 2022-06
    93/100 stars

    Images

    1) Product Images from "Carbon Nanotube Scaffolds Tune Synaptic Strength in Cultured Neural Circuits: Novel Frontiers in Nanomaterial–Tissue Interactions"

    Article Title: Carbon Nanotube Scaffolds Tune Synaptic Strength in Cultured Neural Circuits: Novel Frontiers in Nanomaterial–Tissue Interactions

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1332-11.2011

    MWCNT substrates increase the number of GABAergic synaptic contacts and tune PSC amplitude without affecting PSC kinetics. A , Confocal images of hippocampal neurons in culture (9 d in vitro ) in control ( a ) and CNT ( b ) identifying the presynaptic VGAT (green) and the postsynaptic γ2 subunit of GABA A receptors (red). a , Hippocampal neurons cultured on untreated glass coverslips. Scale bar: 10 μm. a1–a3 , Higher magnification of the region highlighted by the white box in a showing the VGAT (green) and GABA A receptor γ2 subunit (red) clusters. Colocalization of VGAT and GABA A γ2 clusters identify synapses. Scale bar: 2 μm. b , Hippocampal CNT neurons. Scale bar: 10 μm. b1–b3 , Higher magnification of the region highlighted by the white box in b . Note the increased number of the VGAT (green) and GABA A receptor γ2 subunit (red) clusters and of colocalized VGAT and GABA A γ2 clusters. Scale bar: 2 μm. c , Histograms summarize results from three different culture series. CNT neurons display 1.6 times as many synapses compared to control neurons (252.0 ± 20.1 and 401.3 ± 31.5 synapses in control and CNT, respectively; n = 24 and n = 28 fields for control and CNT, respectively; ** p
    Figure Legend Snippet: MWCNT substrates increase the number of GABAergic synaptic contacts and tune PSC amplitude without affecting PSC kinetics. A , Confocal images of hippocampal neurons in culture (9 d in vitro ) in control ( a ) and CNT ( b ) identifying the presynaptic VGAT (green) and the postsynaptic γ2 subunit of GABA A receptors (red). a , Hippocampal neurons cultured on untreated glass coverslips. Scale bar: 10 μm. a1–a3 , Higher magnification of the region highlighted by the white box in a showing the VGAT (green) and GABA A receptor γ2 subunit (red) clusters. Colocalization of VGAT and GABA A γ2 clusters identify synapses. Scale bar: 2 μm. b , Hippocampal CNT neurons. Scale bar: 10 μm. b1–b3 , Higher magnification of the region highlighted by the white box in b . Note the increased number of the VGAT (green) and GABA A receptor γ2 subunit (red) clusters and of colocalized VGAT and GABA A γ2 clusters. Scale bar: 2 μm. c , Histograms summarize results from three different culture series. CNT neurons display 1.6 times as many synapses compared to control neurons (252.0 ± 20.1 and 401.3 ± 31.5 synapses in control and CNT, respectively; n = 24 and n = 28 fields for control and CNT, respectively; ** p

    Techniques Used: In Vitro, Cell Culture

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  • 93
    Alomone Labs γ2 subunit
    MWCNT substrates increase the number of GABAergic synaptic contacts and tune PSC amplitude without affecting PSC kinetics. A , Confocal images of hippocampal neurons in culture (9 d in vitro ) in control ( a ) and CNT ( b ) identifying the presynaptic VGAT (green) and the postsynaptic <t>γ2</t> subunit of GABA A receptors (red). a , Hippocampal neurons cultured on untreated glass coverslips. Scale bar: 10 μm. a1–a3 , Higher magnification of the region highlighted by the white box in a showing the VGAT (green) and GABA A receptor γ2 subunit (red) clusters. Colocalization of VGAT and GABA A γ2 clusters identify synapses. Scale bar: 2 μm. b , Hippocampal CNT neurons. Scale bar: 10 μm. b1–b3 , Higher magnification of the region highlighted by the white box in b . Note the increased number of the VGAT (green) and GABA A receptor γ2 subunit (red) clusters and of colocalized VGAT and GABA A γ2 clusters. Scale bar: 2 μm. c , Histograms summarize results from three different culture series. CNT neurons display 1.6 times as many synapses compared to control neurons (252.0 ± 20.1 and 401.3 ± 31.5 synapses in control and CNT, respectively; n = 24 and n = 28 fields for control and CNT, respectively; ** p
    γ2 Subunit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/γ2 subunit/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    γ2 subunit - by Bioz Stars, 2022-06
    93/100 stars
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    MWCNT substrates increase the number of GABAergic synaptic contacts and tune PSC amplitude without affecting PSC kinetics. A , Confocal images of hippocampal neurons in culture (9 d in vitro ) in control ( a ) and CNT ( b ) identifying the presynaptic VGAT (green) and the postsynaptic γ2 subunit of GABA A receptors (red). a , Hippocampal neurons cultured on untreated glass coverslips. Scale bar: 10 μm. a1–a3 , Higher magnification of the region highlighted by the white box in a showing the VGAT (green) and GABA A receptor γ2 subunit (red) clusters. Colocalization of VGAT and GABA A γ2 clusters identify synapses. Scale bar: 2 μm. b , Hippocampal CNT neurons. Scale bar: 10 μm. b1–b3 , Higher magnification of the region highlighted by the white box in b . Note the increased number of the VGAT (green) and GABA A receptor γ2 subunit (red) clusters and of colocalized VGAT and GABA A γ2 clusters. Scale bar: 2 μm. c , Histograms summarize results from three different culture series. CNT neurons display 1.6 times as many synapses compared to control neurons (252.0 ± 20.1 and 401.3 ± 31.5 synapses in control and CNT, respectively; n = 24 and n = 28 fields for control and CNT, respectively; ** p

    Journal: The Journal of Neuroscience

    Article Title: Carbon Nanotube Scaffolds Tune Synaptic Strength in Cultured Neural Circuits: Novel Frontiers in Nanomaterial–Tissue Interactions

    doi: 10.1523/JNEUROSCI.1332-11.2011

    Figure Lengend Snippet: MWCNT substrates increase the number of GABAergic synaptic contacts and tune PSC amplitude without affecting PSC kinetics. A , Confocal images of hippocampal neurons in culture (9 d in vitro ) in control ( a ) and CNT ( b ) identifying the presynaptic VGAT (green) and the postsynaptic γ2 subunit of GABA A receptors (red). a , Hippocampal neurons cultured on untreated glass coverslips. Scale bar: 10 μm. a1–a3 , Higher magnification of the region highlighted by the white box in a showing the VGAT (green) and GABA A receptor γ2 subunit (red) clusters. Colocalization of VGAT and GABA A γ2 clusters identify synapses. Scale bar: 2 μm. b , Hippocampal CNT neurons. Scale bar: 10 μm. b1–b3 , Higher magnification of the region highlighted by the white box in b . Note the increased number of the VGAT (green) and GABA A receptor γ2 subunit (red) clusters and of colocalized VGAT and GABA A γ2 clusters. Scale bar: 2 μm. c , Histograms summarize results from three different culture series. CNT neurons display 1.6 times as many synapses compared to control neurons (252.0 ± 20.1 and 401.3 ± 31.5 synapses in control and CNT, respectively; n = 24 and n = 28 fields for control and CNT, respectively; ** p

    Article Snippet: To label only surface GABAA receptors, neurons were incubated with (rabbit) polyclonal antibody against the N-terminal of γ2 subunit (1:100; Alomone Labs) for 1 h. They were then permeabilized with 0.1% Triton X-100 in PBS for 2 min and blocked again in 1% FBS in PBS for 30 min. After incubation with (mouse) monoclonal antibody against the vesicular GABA transporter (VGAT; 1:200; Synaptic Systems) for 1 h, they were incubated with a mixture of secondary antibodies (Alexa Fluor 488 goat anti-mouse, 1:500 and Alexa Fluor 647 goat anti-rabbit, 1:500) for 45 min.

    Techniques: In Vitro, Cell Culture