α3  (Alomone Labs)


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    • 93
    Name:
    Anti GABA A alpha3 Receptor extracellular Antibody
    Description:
    Anti GABA A alpha3 Receptor extracellular Antibody is directed against an epitope of human GABA A α3 receptor Anti GABA A α3 Receptor extracellular Antibody AGA 003 can be used in western blot immunoprecipitation immunocytochemistry and immunohistochemistry applications The antibody recognizes an extracellular epitope and is highly suited to detect GABRA3 in living cells It has been designed to recognize GABRA3 from rat mouse and human samples
    Catalog Number:
    AGA-003
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    		Buy from Supplier

    Structured Review

    Alomone Labs α3
    Anti GABA A alpha3 Receptor extracellular Antibody
    Anti GABA A alpha3 Receptor extracellular Antibody is directed against an epitope of human GABA A α3 receptor Anti GABA A α3 Receptor extracellular Antibody AGA 003 can be used in western blot immunoprecipitation immunocytochemistry and immunohistochemistry applications The antibody recognizes an extracellular epitope and is highly suited to detect GABRA3 in living cells It has been designed to recognize GABRA3 from rat mouse and human samples
    https://www.bioz.com/result/α3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α3 - by Bioz Stars, 2021-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Reticular nucleus-specific changes in ?3 subunit protein at GABA synapses in genetically epilepsy-prone rats
    Article Snippet: .. Sections were incubated in preimmune serum to block nonspecific staining and were then placed in the following primary antibodies for 48 h at 4°C: anti-GABAA R α1or α3 subunit (rabbit polyclonal antibodies, diluted 1:200 to 1:500; Alomone Labs, Jerusalem, Israel), anti-GABAA R β2/β3 subunits (a monoclonal antibody that recognizes both β2 and β3 subunit epitopes, diluted 1:400, a gift from Dr. Angel L. de Blas, University of Connecticut, Storrs, CT). ..

    Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling
    Article Snippet: .. To probe GABA-A subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody (Alomone labs), rabbit anti-GABA-A R α5 polyclonal antibody (Synaptic system), and mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab) ON at 4°C. ..

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites
    Article Snippet: .. To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C. ..

    Blocking Assay:

    Article Title: Reticular nucleus-specific changes in ?3 subunit protein at GABA synapses in genetically epilepsy-prone rats
    Article Snippet: .. Sections were incubated in preimmune serum to block nonspecific staining and were then placed in the following primary antibodies for 48 h at 4°C: anti-GABAA R α1or α3 subunit (rabbit polyclonal antibodies, diluted 1:200 to 1:500; Alomone Labs, Jerusalem, Israel), anti-GABAA R β2/β3 subunits (a monoclonal antibody that recognizes both β2 and β3 subunit epitopes, diluted 1:400, a gift from Dr. Angel L. de Blas, University of Connecticut, Storrs, CT). ..

    Staining:

    Article Title: Reticular nucleus-specific changes in ?3 subunit protein at GABA synapses in genetically epilepsy-prone rats
    Article Snippet: .. Sections were incubated in preimmune serum to block nonspecific staining and were then placed in the following primary antibodies for 48 h at 4°C: anti-GABAA R α1or α3 subunit (rabbit polyclonal antibodies, diluted 1:200 to 1:500; Alomone Labs, Jerusalem, Israel), anti-GABAA R β2/β3 subunits (a monoclonal antibody that recognizes both β2 and β3 subunit epitopes, diluted 1:400, a gift from Dr. Angel L. de Blas, University of Connecticut, Storrs, CT). ..

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    • rabbit anti gaba a r α3 polyclonal antibody  (Alomone Labs)
    93
    Alomone Labs rabbit anti gaba a r α3 polyclonal antibody
    Immunostaining of GABA-A R subunits in primary microglia. Representative micrographs of unchallenged primary microglia stained with antibodies against GABA-A R (A) <t>α3</t> subunit, (B) α5 subunit, and (C) β3 subunit, respectively, together with Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei), as indicated under Materials and Methods. Representative data is shown from 4 independent experiments. Scale bars, for (A,B) = 10 μm, for (C) = 5 μm.
    Rabbit Anti Gaba A R α3 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gaba a r α3 polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gaba a r α3 polyclonal antibody - by Bioz Stars, 2021-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Immunostaining of GABA-A R subunits in primary microglia. Representative micrographs of unchallenged primary microglia stained with antibodies against GABA-A R (A) α3 subunit, (B) α5 subunit, and (C) β3 subunit, respectively, together with Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei), as indicated under Materials and Methods. Representative data is shown from 4 independent experiments. Scale bars, for (A,B) = 10 μm, for (C) = 5 μm.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling

    doi: 10.3389/fcimb.2019.00073

    Figure Lengend Snippet: Immunostaining of GABA-A R subunits in primary microglia. Representative micrographs of unchallenged primary microglia stained with antibodies against GABA-A R (A) α3 subunit, (B) α5 subunit, and (C) β3 subunit, respectively, together with Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei), as indicated under Materials and Methods. Representative data is shown from 4 independent experiments. Scale bars, for (A,B) = 10 μm, for (C) = 5 μm.

    Article Snippet: To probe GABA-A subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody (Alomone labs), rabbit anti-GABA-A R α5 polyclonal antibody (Synaptic system), and mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab) ON at 4°C.

    Techniques: Immunostaining, Staining

    Modulated transcriptional expression of GABAergic and VDCC signaling components in Toxoplasma -challenged primary microglia. The relative expression (2 −Δ Ct ) for each target gene in Toxoplasma (PTG)-challenged primary microglia was normalized to that of in unchallenged microglia and the differences are represented as percentage increase (red color intensity scale) or decrease (blue scale) in the heat map. For both conditions, unchallenged and Toxoplasma -challenged, data is represented as mean of 2–4 independent experiments at indicated time points. The modulation of mRNA expression is shown for (A) GABA synthesis and degrading enzymes, (B) GABA transporters, (C) GABA-A R subunits, (D) CCCs, and (E) VDCCs.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling

    doi: 10.3389/fcimb.2019.00073

    Figure Lengend Snippet: Modulated transcriptional expression of GABAergic and VDCC signaling components in Toxoplasma -challenged primary microglia. The relative expression (2 −Δ Ct ) for each target gene in Toxoplasma (PTG)-challenged primary microglia was normalized to that of in unchallenged microglia and the differences are represented as percentage increase (red color intensity scale) or decrease (blue scale) in the heat map. For both conditions, unchallenged and Toxoplasma -challenged, data is represented as mean of 2–4 independent experiments at indicated time points. The modulation of mRNA expression is shown for (A) GABA synthesis and degrading enzymes, (B) GABA transporters, (C) GABA-A R subunits, (D) CCCs, and (E) VDCCs.

    Article Snippet: To probe GABA-A subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody (Alomone labs), rabbit anti-GABA-A R α5 polyclonal antibody (Synaptic system), and mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab) ON at 4°C.

    Techniques: Expressing

    Representative Western blots show detection of weak or undetectable polypeptide bands for GABA-A R subunits (alpha3, alpha5, rho1) in mBMDCs with commercially available antibodies.

    Journal: eLife

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

    doi: 10.7554/eLife.60528

    Figure Lengend Snippet: Representative Western blots show detection of weak or undetectable polypeptide bands for GABA-A R subunits (alpha3, alpha5, rho1) in mBMDCs with commercially available antibodies.

    Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

    Techniques: Western Blot

    Effect of pharmacological inhibitors of GABA-A Rs, NKCC1 and VDCCs on hypermotility. ( A, B ) Box-and-whisker dot plots show median velocities (μm/min) of mBMDCs challenged with N. caninum Nc-1 strain ( A ) and Nc-Liverpool strain ( B ) and treated with GABA-A R inhibitors, NKCC1 inhibitor or VDCC inhibitors as indicated, respectively (n = 3 independent experiments). ( C ) Box-and-whisker dot plots show median velocities (μm/min) of hMonocytes challenged with T. gondii (PRU) and treated with GABA-A R inhibitor, NKCC1 inhibitor or VDCC inhibitor, respectively (n = 3 independent experiments). Statistical significance was assessed by ANOVA with Dunnett’s multiple comparison test for ( A, B, C ), ***p

    Journal: eLife

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

    doi: 10.7554/eLife.60528

    Figure Lengend Snippet: Effect of pharmacological inhibitors of GABA-A Rs, NKCC1 and VDCCs on hypermotility. ( A, B ) Box-and-whisker dot plots show median velocities (μm/min) of mBMDCs challenged with N. caninum Nc-1 strain ( A ) and Nc-Liverpool strain ( B ) and treated with GABA-A R inhibitors, NKCC1 inhibitor or VDCC inhibitors as indicated, respectively (n = 3 independent experiments). ( C ) Box-and-whisker dot plots show median velocities (μm/min) of hMonocytes challenged with T. gondii (PRU) and treated with GABA-A R inhibitor, NKCC1 inhibitor or VDCC inhibitor, respectively (n = 3 independent experiments). Statistical significance was assessed by ANOVA with Dunnett’s multiple comparison test for ( A, B, C ), ***p

    Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

    Techniques: Whisker Assay