α3  (Alomone Labs)

Journal: eLife

Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

doi: 10.7554/eLife.60528

Figure Lengend Snippet: Representative Western blots show detection of weak or undetectable polypeptide bands for GABA-A R subunits (alpha3, alpha5, rho1) in mBMDCs with commercially available antibodies.

Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

Techniques: Western Blot

Journal: eLife

Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

doi: 10.7554/eLife.60528

Figure Lengend Snippet: Effect of pharmacological inhibitors of GABA-A Rs, NKCC1 and VDCCs on hypermotility. ( A, B ) Box-and-whisker dot plots show median velocities (μm/min) of mBMDCs challenged with N. caninum Nc-1 strain ( A ) and Nc-Liverpool strain ( B ) and treated with GABA-A R inhibitors, NKCC1 inhibitor or VDCC inhibitors as indicated, respectively (n = 3 independent experiments). ( C ) Box-and-whisker dot plots show median velocities (μm/min) of hMonocytes challenged with T. gondii (PRU) and treated with GABA-A R inhibitor, NKCC1 inhibitor or VDCC inhibitor, respectively (n = 3 independent experiments). Statistical significance was assessed by ANOVA with Dunnett’s multiple comparison test for ( A, B, C ), ***p

Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

Techniques: Whisker Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling

doi: 10.3389/fcimb.2019.00073

Figure Lengend Snippet: Immunostaining of GABA-A R subunits in primary microglia. Representative micrographs of unchallenged primary microglia stained with antibodies against GABA-A R (A) α3 subunit, (B) α5 subunit, and (C) β3 subunit, respectively, together with Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei), as indicated under Materials and Methods. Representative data is shown from 4 independent experiments. Scale bars, for (A,B) = 10 μm, for (C) = 5 μm.

Article Snippet: To probe GABA-A subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody (Alomone labs), rabbit anti-GABA-A R α5 polyclonal antibody (Synaptic system), and mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab) ON at 4°C.

Techniques: Immunostaining, Staining

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling

doi: 10.3389/fcimb.2019.00073

Figure Lengend Snippet: Modulated transcriptional expression of GABAergic and VDCC signaling components in Toxoplasma -challenged primary microglia. The relative expression (2 −Δ Ct ) for each target gene in Toxoplasma (PTG)-challenged primary microglia was normalized to that of in unchallenged microglia and the differences are represented as percentage increase (red color intensity scale) or decrease (blue scale) in the heat map. For both conditions, unchallenged and Toxoplasma -challenged, data is represented as mean of 2–4 independent experiments at indicated time points. The modulation of mRNA expression is shown for (A) GABA synthesis and degrading enzymes, (B) GABA transporters, (C) GABA-A R subunits, (D) CCCs, and (E) VDCCs.

Article Snippet: To probe GABA-A subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody (Alomone labs), rabbit anti-GABA-A R α5 polyclonal antibody (Synaptic system), and mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab) ON at 4°C.

Techniques: Expressing