ryr1  (Alomone Labs)


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    Structured Review

    Alomone Labs ryr1
    Fibers with elevated <t>RYR1</t> contain fast myosin. Shown is a field from a tibialis anterior muscle double labeled for myosin II and RYR1. All 3 fibers in the field are positive for fast myosin. In the lower center of the field is a small fiber that stains
    Ryr1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Upregulation of the CaV 1.1-ryanodine receptor complex in a rat model of critical illness myopathy"

    Article Title: Upregulation of the CaV 1.1-ryanodine receptor complex in a rat model of critical illness myopathy

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    doi: 10.1152/ajpregu.00032.2011

    Fibers with elevated RYR1 contain fast myosin. Shown is a field from a tibialis anterior muscle double labeled for myosin II and RYR1. All 3 fibers in the field are positive for fast myosin. In the lower center of the field is a small fiber that stains
    Figure Legend Snippet: Fibers with elevated RYR1 contain fast myosin. Shown is a field from a tibialis anterior muscle double labeled for myosin II and RYR1. All 3 fibers in the field are positive for fast myosin. In the lower center of the field is a small fiber that stains

    Techniques Used: Labeling

    Marked elevation of RYR1 in a subset of fibers. A : RYR1 staining in 2 fibers from the tibialis anterior muscle in a control rat. The staining is well organized into parallel stripes running perpendicular to the length of the fiber. B : RYR1 staining in
    Figure Legend Snippet: Marked elevation of RYR1 in a subset of fibers. A : RYR1 staining in 2 fibers from the tibialis anterior muscle in a control rat. The staining is well organized into parallel stripes running perpendicular to the length of the fiber. B : RYR1 staining in

    Techniques Used: Staining

    Expression of the ryanodine receptor (RYR) increases in critical illness myopathy (CIM).  A : skeletal muscle membranes prepared from individual control (Con) or CIM animals were analyzed in Western blot analysis using a monoclonal antibody to RYR. There
    Figure Legend Snippet: Expression of the ryanodine receptor (RYR) increases in critical illness myopathy (CIM). A : skeletal muscle membranes prepared from individual control (Con) or CIM animals were analyzed in Western blot analysis using a monoclonal antibody to RYR. There

    Techniques Used: Expressing, Western Blot

    Voltage-gated calcium channel type 1.1 (Ca V 1.1) and RYR1 are upregulated in the same fibers in CIM. Two fields from an individual tibialis anterior muscle double labeled for Ca V 1.1 and RYR1 are shown. In the field at the top , a severely atrophied muscle
    Figure Legend Snippet: Voltage-gated calcium channel type 1.1 (Ca V 1.1) and RYR1 are upregulated in the same fibers in CIM. Two fields from an individual tibialis anterior muscle double labeled for Ca V 1.1 and RYR1 are shown. In the field at the top , a severely atrophied muscle

    Techniques Used: Labeling

    Calpain II is elevated in fibers with elevated RYR1. Shown is a field from a tibialis anterior muscle double labeled for calpain II and RYR1. In the field are two atrophied fibers that have elevated levels of both calpain II and RYR1. The normal fibers
    Figure Legend Snippet: Calpain II is elevated in fibers with elevated RYR1. Shown is a field from a tibialis anterior muscle double labeled for calpain II and RYR1. In the field are two atrophied fibers that have elevated levels of both calpain II and RYR1. The normal fibers

    Techniques Used: Labeling

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    Alomone Labs rabbit anti nr1
    Exposure to high glucose (HG) increases expression of NMDA receptor subunits in primary cultures of rat mesangial cells. A : Representative results of RT-PCR showing significantly increased abundance of transcripts encoding <t>NR1,</t> NR2B, and NR2C subunits but not of NR2A or NR2D in cells cultured for 24 h in HG medium compared with cells cultured in normal glucose (control [Con]). B : Densitometric analysis of three repetitions of the experiments shown in A . C : Immunoblot analysis showing increased abundance of NMDA receptor subunits in primary cultures of rat mesangial cells cultured in HG. D : Densitometric analysis of three repetitions of the experiments shown in C . Data are mean ± SD. * P
    Rabbit Anti Nr1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Alomone Labs polyclonal anti endothelin receptor a
    The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10 −10 to 10 −9 ET-1. B: This coincides with a significant increase in the ET A R in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 ( B ) or n = 16 to 35 profiles per group across 4 to 5 mice ( A ) in two independent experiments. ∗ P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ET A R, <t>endothelin</t> receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.
    Polyclonal Anti Endothelin Receptor A, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Alomone Labs antigen preabsorbed anti clc 3 ab
    <t>Anti-ClC-3</t> Ab dialysis abolishes expressed I gpClC-3 in NIH/3T3 cells A , phase contrast (left panel) and fluorescence micrographs (right panel) of NIH/3T3 cell transfected with gpClC-3-GFP. B , representative I gpClC-3 recorded from GFP-positive NIH/3T3 cells (as shown in A ) under isotonic, hypotonic and hypertonic conditions over the range -100 to +120 mV. C , effects of anti-ClC-3 Ab on I gpClC-3 at ±80 mV when pipette solutions contained <t>preabsorbed</t> anti-ClC-3 Ab (control, ○) or anti-ClC-3 Ab alone (▪). D , mean current densities recorded from untransfected NIH/3T3 cells (blue, n = 38), gpClC-3-GFP-transfected cells dialysed with standard intracellular pipette solutions (green, n = 9), preabsorbed anti-ClC-3 Ab (black, n = 4), and anti-ClC-3 Ab alone (red, n = 9).
    Antigen Preabsorbed Anti Clc 3 Ab, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen preabsorbed anti clc 3 ab/product/Alomone Labs
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    Image Search Results


    Exposure to high glucose (HG) increases expression of NMDA receptor subunits in primary cultures of rat mesangial cells. A : Representative results of RT-PCR showing significantly increased abundance of transcripts encoding NR1, NR2B, and NR2C subunits but not of NR2A or NR2D in cells cultured for 24 h in HG medium compared with cells cultured in normal glucose (control [Con]). B : Densitometric analysis of three repetitions of the experiments shown in A . C : Immunoblot analysis showing increased abundance of NMDA receptor subunits in primary cultures of rat mesangial cells cultured in HG. D : Densitometric analysis of three repetitions of the experiments shown in C . Data are mean ± SD. * P

    Journal: Diabetes

    Article Title: NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801

    doi: 10.2337/db16-0209

    Figure Lengend Snippet: Exposure to high glucose (HG) increases expression of NMDA receptor subunits in primary cultures of rat mesangial cells. A : Representative results of RT-PCR showing significantly increased abundance of transcripts encoding NR1, NR2B, and NR2C subunits but not of NR2A or NR2D in cells cultured for 24 h in HG medium compared with cells cultured in normal glucose (control [Con]). B : Densitometric analysis of three repetitions of the experiments shown in A . C : Immunoblot analysis showing increased abundance of NMDA receptor subunits in primary cultures of rat mesangial cells cultured in HG. D : Densitometric analysis of three repetitions of the experiments shown in C . Data are mean ± SD. * P

    Article Snippet: Primary antibodies were rabbit anti-NR1 (AGC-001 1:100; Alomone Labs) or rabbit anti-NR2A (AGC-002 1:100; Alomone Labs) for 24 h at 4°C.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Cell Culture

    Increased expression of NMDA receptor subunits in renal cortex of Akita mice. A : Representative results of RT-PCR showing increased abundance of transcripts encoding NR1, NR2A, and NR2C subunits but not in NR2B or NR2D in renal cortex in 12-week-old Akita mice compared with 12-week-old DBA/2J control mice. B : Densitometric analysis from four mice per group. C : Immunoblot analysis showing increased abundance of NMDA receptor subunits in Akita mice compared with DBA/2J control mice. D : Densitometric analysis from four mice per group. Data are mean ± SD. * P

    Journal: Diabetes

    Article Title: NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801

    doi: 10.2337/db16-0209

    Figure Lengend Snippet: Increased expression of NMDA receptor subunits in renal cortex of Akita mice. A : Representative results of RT-PCR showing increased abundance of transcripts encoding NR1, NR2A, and NR2C subunits but not in NR2B or NR2D in renal cortex in 12-week-old Akita mice compared with 12-week-old DBA/2J control mice. B : Densitometric analysis from four mice per group. C : Immunoblot analysis showing increased abundance of NMDA receptor subunits in Akita mice compared with DBA/2J control mice. D : Densitometric analysis from four mice per group. Data are mean ± SD. * P

    Article Snippet: Primary antibodies were rabbit anti-NR1 (AGC-001 1:100; Alomone Labs) or rabbit anti-NR2A (AGC-002 1:100; Alomone Labs) for 24 h at 4°C.

    Techniques: Expressing, Mouse Assay, Reverse Transcription Polymerase Chain Reaction

    Immunohistochemistry (IHC) suggests increased abundance of NMDA receptor subunits throughout the kidney of 12-week-old Akita mice. IHC was carried out in paraffin sections. Negative control sections shown at the top were not exposed to a primary antibody. A : Especially large increases in NR1, NR2A, and NR2C in renal tubules. B : Signal in glomeruli for NR1, NR2A, and NR2C. Primary processes were visible in some of the cells within glomeruli. C : Staining intensity per square micron in the whole kidney (top) and within glomeruli (bottom). Data are mean ± SD. * P

    Journal: Diabetes

    Article Title: NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801

    doi: 10.2337/db16-0209

    Figure Lengend Snippet: Immunohistochemistry (IHC) suggests increased abundance of NMDA receptor subunits throughout the kidney of 12-week-old Akita mice. IHC was carried out in paraffin sections. Negative control sections shown at the top were not exposed to a primary antibody. A : Especially large increases in NR1, NR2A, and NR2C in renal tubules. B : Signal in glomeruli for NR1, NR2A, and NR2C. Primary processes were visible in some of the cells within glomeruli. C : Staining intensity per square micron in the whole kidney (top) and within glomeruli (bottom). Data are mean ± SD. * P

    Article Snippet: Primary antibodies were rabbit anti-NR1 (AGC-001 1:100; Alomone Labs) or rabbit anti-NR2A (AGC-002 1:100; Alomone Labs) for 24 h at 4°C.

    Techniques: Immunohistochemistry, Mouse Assay, Negative Control, Staining

    Exposure to high glucose (HG) increases expression of NMDA receptor subunits in cultured mouse podocytes (MPC-5 cells). A : Representative results of RT-PCR showing increased abundance of transcripts encoding NR1, NR2A, NR2B, and NR2C subunits in cells cultured for 24 h in a medium containing 25 mmol/L glucose (HG). There was no change in NR2D. Control cells (Con) were cultured in medium containing 9 mmol/L glucose, with 16 mmol/L mannitol as an osmotic control. B : Densitometric analysis of three repetitions of the experiments shown in A . C : Immunoblot analysis showing increased abundance of NMDA receptor subunits in podocytes cultured in HG compared with Con. D : Densitometric analysis of three repetitions of the experiments shown in C . Data are mean ± SD. * P

    Journal: Diabetes

    Article Title: NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801

    doi: 10.2337/db16-0209

    Figure Lengend Snippet: Exposure to high glucose (HG) increases expression of NMDA receptor subunits in cultured mouse podocytes (MPC-5 cells). A : Representative results of RT-PCR showing increased abundance of transcripts encoding NR1, NR2A, NR2B, and NR2C subunits in cells cultured for 24 h in a medium containing 25 mmol/L glucose (HG). There was no change in NR2D. Control cells (Con) were cultured in medium containing 9 mmol/L glucose, with 16 mmol/L mannitol as an osmotic control. B : Densitometric analysis of three repetitions of the experiments shown in A . C : Immunoblot analysis showing increased abundance of NMDA receptor subunits in podocytes cultured in HG compared with Con. D : Densitometric analysis of three repetitions of the experiments shown in C . Data are mean ± SD. * P

    Article Snippet: Primary antibodies were rabbit anti-NR1 (AGC-001 1:100; Alomone Labs) or rabbit anti-NR2A (AGC-002 1:100; Alomone Labs) for 24 h at 4°C.

    Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

    The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10 −10 to 10 −9 ET-1. B: This coincides with a significant increase in the ET A R in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 ( B ) or n = 16 to 35 profiles per group across 4 to 5 mice ( A ) in two independent experiments. ∗ P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ET A R, endothelin receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

    Journal: The American Journal of Pathology

    Article Title: Vascular Dysfunction in Pneumocystis-Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

    doi: 10.1016/j.ajpath.2015.10.008

    Figure Lengend Snippet: The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10 −10 to 10 −9 ET-1. B: This coincides with a significant increase in the ET A R in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 ( B ) or n = 16 to 35 profiles per group across 4 to 5 mice ( A ) in two independent experiments. ∗ P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ET A R, endothelin receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

    Article Snippet: Polyclonal anti-endothelin receptor A (ETA R) and the control peptide to verify specificity were from Alomone Labs (no. AER-001; Jerusalem, Israel).

    Techniques: Mouse Assay, Western Blot

    Anti-ClC-3 Ab dialysis abolishes expressed I gpClC-3 in NIH/3T3 cells A , phase contrast (left panel) and fluorescence micrographs (right panel) of NIH/3T3 cell transfected with gpClC-3-GFP. B , representative I gpClC-3 recorded from GFP-positive NIH/3T3 cells (as shown in A ) under isotonic, hypotonic and hypertonic conditions over the range -100 to +120 mV. C , effects of anti-ClC-3 Ab on I gpClC-3 at ±80 mV when pipette solutions contained preabsorbed anti-ClC-3 Ab (control, ○) or anti-ClC-3 Ab alone (▪). D , mean current densities recorded from untransfected NIH/3T3 cells (blue, n = 38), gpClC-3-GFP-transfected cells dialysed with standard intracellular pipette solutions (green, n = 9), preabsorbed anti-ClC-3 Ab (black, n = 4), and anti-ClC-3 Ab alone (red, n = 9).

    Journal: The Journal of Physiology

    Article Title: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody

    doi: 10.1111/j.1469-7793.2001.0437i.x

    Figure Lengend Snippet: Anti-ClC-3 Ab dialysis abolishes expressed I gpClC-3 in NIH/3T3 cells A , phase contrast (left panel) and fluorescence micrographs (right panel) of NIH/3T3 cell transfected with gpClC-3-GFP. B , representative I gpClC-3 recorded from GFP-positive NIH/3T3 cells (as shown in A ) under isotonic, hypotonic and hypertonic conditions over the range -100 to +120 mV. C , effects of anti-ClC-3 Ab on I gpClC-3 at ±80 mV when pipette solutions contained preabsorbed anti-ClC-3 Ab (control, ○) or anti-ClC-3 Ab alone (▪). D , mean current densities recorded from untransfected NIH/3T3 cells (blue, n = 38), gpClC-3-GFP-transfected cells dialysed with standard intracellular pipette solutions (green, n = 9), preabsorbed anti-ClC-3 Ab (black, n = 4), and anti-ClC-3 Ab alone (red, n = 9).

    Article Snippet: shows a phase contrast (left) and fluorescence micrograph (right) of a NIH/3T3 cell transiently co-transfected by electroporation with wild-type gpClC-3 and the GFP reporter plasmid (arrow). gpClC-3-GFP-transfected cells in symmetrical Cl− (135 mM) conditions, generated large volume-sensitive outwardly rectifying whole-cell Cl− currents ( ) that were similar to currents previously recorded from NIH/3T3 cells transfected with gpClC-3 alone ( ). illustrates the effects of intracellular dialysis of either anti-ClC-3 Ab alone or antigen-preabsorbed anti-ClC-3 Ab on the amplitude and time course of I gpClC-3 activated or inhibited by exposure to hypotonic and hypertonic solutions, respectively, in gpClC-3-GFP-transfected 3T3 cells.

    Techniques: Fluorescence, Transfection, Transferring

    Anti-ClC-3 Ab abolishes native VSOAC currents in canine PASMCs A , time course of VSOAC currents from two PASMCs dialysed with either anti-ClC-3 Ab (5 μg ml −1 ) preabsorbed with antigen (50 μg ml −1 ; ○), or anti-ClC-3 Ab alone (5 μg ml −1 ; •). Inset: Western blot analysis of native ClC-3 expression in isolated canine PASMCs. B , mean current densities in cells dialysed with either standard intracellular solutions ( n = 11), preabsorbed anti-ClC-3 Ab ( n = 4) or anti-ClC-3 Ab alone ( n = 5).

    Journal: The Journal of Physiology

    Article Title: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody

    doi: 10.1111/j.1469-7793.2001.0437i.x

    Figure Lengend Snippet: Anti-ClC-3 Ab abolishes native VSOAC currents in canine PASMCs A , time course of VSOAC currents from two PASMCs dialysed with either anti-ClC-3 Ab (5 μg ml −1 ) preabsorbed with antigen (50 μg ml −1 ; ○), or anti-ClC-3 Ab alone (5 μg ml −1 ; •). Inset: Western blot analysis of native ClC-3 expression in isolated canine PASMCs. B , mean current densities in cells dialysed with either standard intracellular solutions ( n = 11), preabsorbed anti-ClC-3 Ab ( n = 4) or anti-ClC-3 Ab alone ( n = 5).

    Article Snippet: shows a phase contrast (left) and fluorescence micrograph (right) of a NIH/3T3 cell transiently co-transfected by electroporation with wild-type gpClC-3 and the GFP reporter plasmid (arrow). gpClC-3-GFP-transfected cells in symmetrical Cl− (135 mM) conditions, generated large volume-sensitive outwardly rectifying whole-cell Cl− currents ( ) that were similar to currents previously recorded from NIH/3T3 cells transfected with gpClC-3 alone ( ). illustrates the effects of intracellular dialysis of either anti-ClC-3 Ab alone or antigen-preabsorbed anti-ClC-3 Ab on the amplitude and time course of I gpClC-3 activated or inhibited by exposure to hypotonic and hypertonic solutions, respectively, in gpClC-3-GFP-transfected 3T3 cells.

    Techniques: Western Blot, Expressing, Isolation

    Anti-ClC-3 Ab dialysis abolishes native VSOAC currents in guinea-pig cardiac myocytes A , native VSOAC currents at ±80 mV in myocytes exposed to isotonic and hypotonic solutions. Aa , pipette solutions contained preabsorbed anti-ClC-3 Ab (control, ○) or anti-ClC-3 Ab alone (▪). Ab , mean current densities for preabsorbed anti-ClC-3 Ab (□, n = 3) or anti-ClC-3 Ab alone (▪, n = 5) at times indicated. B, same as A , except cells were preswelled by pre-exposure to hypotonic bath solutions prior to membrane rupture and dialysis; preabsorbed anti-ClC-3 Ab (□, n = 4), and anti-ClC-3 Ab alone (▪, n = 5).

    Journal: The Journal of Physiology

    Article Title: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody

    doi: 10.1111/j.1469-7793.2001.0437i.x

    Figure Lengend Snippet: Anti-ClC-3 Ab dialysis abolishes native VSOAC currents in guinea-pig cardiac myocytes A , native VSOAC currents at ±80 mV in myocytes exposed to isotonic and hypotonic solutions. Aa , pipette solutions contained preabsorbed anti-ClC-3 Ab (control, ○) or anti-ClC-3 Ab alone (▪). Ab , mean current densities for preabsorbed anti-ClC-3 Ab (□, n = 3) or anti-ClC-3 Ab alone (▪, n = 5) at times indicated. B, same as A , except cells were preswelled by pre-exposure to hypotonic bath solutions prior to membrane rupture and dialysis; preabsorbed anti-ClC-3 Ab (□, n = 4), and anti-ClC-3 Ab alone (▪, n = 5).

    Article Snippet: shows a phase contrast (left) and fluorescence micrograph (right) of a NIH/3T3 cell transiently co-transfected by electroporation with wild-type gpClC-3 and the GFP reporter plasmid (arrow). gpClC-3-GFP-transfected cells in symmetrical Cl− (135 mM) conditions, generated large volume-sensitive outwardly rectifying whole-cell Cl− currents ( ) that were similar to currents previously recorded from NIH/3T3 cells transfected with gpClC-3 alone ( ). illustrates the effects of intracellular dialysis of either anti-ClC-3 Ab alone or antigen-preabsorbed anti-ClC-3 Ab on the amplitude and time course of I gpClC-3 activated or inhibited by exposure to hypotonic and hypertonic solutions, respectively, in gpClC-3-GFP-transfected 3T3 cells.

    Techniques: Transferring

    Anti-ClC-3 Ab injection inhibits native VSOAC currents in Xenopus laevis oocytes A , Western blot analysis of native ClC-3 expression in oocytes. B , 4-phorbol 12,13-dibutyrate (PDBu) inhibition of native VSOAC currents in oocytes (-100 to +120 mV). C, time course of VSOAC activation in response to hypotonic bath solutions from a non-injected control oocyte (○) and an oocyte injected with anti-ClC-3 Ab (15 μg ml -1 ; •) at time shown. Cells were held at -30 mV for 30 ms, hyperpolarized to -100 mV for 210 ms and then depolarized to +100 mV for 210 ms, repetitively at 2 Hz. D, mean current densities from control oocytes ( n = 10), oocytes injected with anti-ClC-3 Ab alone (15 μg ml -1 ) ( n = 4) or injected with preabsorbed anti-ClC-3 Ab (150 μg ml -1 ) ( n = 4). Peak current densities were measured after 5 min exposure to isotonic solutions, and after 85 min exposure to hypotonic solutions.

    Journal: The Journal of Physiology

    Article Title: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody

    doi: 10.1111/j.1469-7793.2001.0437i.x

    Figure Lengend Snippet: Anti-ClC-3 Ab injection inhibits native VSOAC currents in Xenopus laevis oocytes A , Western blot analysis of native ClC-3 expression in oocytes. B , 4-phorbol 12,13-dibutyrate (PDBu) inhibition of native VSOAC currents in oocytes (-100 to +120 mV). C, time course of VSOAC activation in response to hypotonic bath solutions from a non-injected control oocyte (○) and an oocyte injected with anti-ClC-3 Ab (15 μg ml -1 ; •) at time shown. Cells were held at -30 mV for 30 ms, hyperpolarized to -100 mV for 210 ms and then depolarized to +100 mV for 210 ms, repetitively at 2 Hz. D, mean current densities from control oocytes ( n = 10), oocytes injected with anti-ClC-3 Ab alone (15 μg ml -1 ) ( n = 4) or injected with preabsorbed anti-ClC-3 Ab (150 μg ml -1 ) ( n = 4). Peak current densities were measured after 5 min exposure to isotonic solutions, and after 85 min exposure to hypotonic solutions.

    Article Snippet: shows a phase contrast (left) and fluorescence micrograph (right) of a NIH/3T3 cell transiently co-transfected by electroporation with wild-type gpClC-3 and the GFP reporter plasmid (arrow). gpClC-3-GFP-transfected cells in symmetrical Cl− (135 mM) conditions, generated large volume-sensitive outwardly rectifying whole-cell Cl− currents ( ) that were similar to currents previously recorded from NIH/3T3 cells transfected with gpClC-3 alone ( ). illustrates the effects of intracellular dialysis of either anti-ClC-3 Ab alone or antigen-preabsorbed anti-ClC-3 Ab on the amplitude and time course of I gpClC-3 activated or inhibited by exposure to hypotonic and hypertonic solutions, respectively, in gpClC-3-GFP-transfected 3T3 cells.

    Techniques: Injection, Western Blot, Expressing, Inhibition, Activation Assay, Mass Spectrometry