rabbit anti fpr2  (Alomone Labs)


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    Alomone Labs rabbit anti fpr2
    Distribution of SPM receptors within minor salivary glands from non-SS and SS patients. (A) <t>ALX/FPR2</t> was expressed in mucous acini from both SS and non-SS patients (white arrows), while it was only observed within striated ducts from SS patients (yellow arrow). (B) BLT1 was found at the plasma membrane of both striated ducts (brown arrows) and mucous acini (pink arrows) of both SS and non-SS patients. (C) CMKLR1 was expressed in mucous acini (purple arrows) as well as striated ducts (orange arrows) of both SS and non-SS patients. Red and green arrows indicate inflammatory and endothelial cells, respectively. A representative image from n = 3 per experimental group is shown. Scale bars represent 50 μm.
    Rabbit Anti Fpr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti fpr2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti fpr2 - by Bioz Stars, 2022-07
    93/100 stars

    Images

    1) Product Images from "Specialized pro-resolving receptors are expressed in salivary glands with Sjögren’s syndrome"

    Article Title: Specialized pro-resolving receptors are expressed in salivary glands with Sjögren’s syndrome

    Journal: Annals of diagnostic pathology

    doi: 10.1016/j.anndiagpath.2021.151865

    Distribution of SPM receptors within minor salivary glands from non-SS and SS patients. (A) ALX/FPR2 was expressed in mucous acini from both SS and non-SS patients (white arrows), while it was only observed within striated ducts from SS patients (yellow arrow). (B) BLT1 was found at the plasma membrane of both striated ducts (brown arrows) and mucous acini (pink arrows) of both SS and non-SS patients. (C) CMKLR1 was expressed in mucous acini (purple arrows) as well as striated ducts (orange arrows) of both SS and non-SS patients. Red and green arrows indicate inflammatory and endothelial cells, respectively. A representative image from n = 3 per experimental group is shown. Scale bars represent 50 μm.
    Figure Legend Snippet: Distribution of SPM receptors within minor salivary glands from non-SS and SS patients. (A) ALX/FPR2 was expressed in mucous acini from both SS and non-SS patients (white arrows), while it was only observed within striated ducts from SS patients (yellow arrow). (B) BLT1 was found at the plasma membrane of both striated ducts (brown arrows) and mucous acini (pink arrows) of both SS and non-SS patients. (C) CMKLR1 was expressed in mucous acini (purple arrows) as well as striated ducts (orange arrows) of both SS and non-SS patients. Red and green arrows indicate inflammatory and endothelial cells, respectively. A representative image from n = 3 per experimental group is shown. Scale bars represent 50 μm.

    Techniques Used:

    2) Product Images from "Resolvin D1 prevents TNF-?-mediated disruption of salivary epithelial formation"

    Article Title: Resolvin D1 prevents TNF-?-mediated disruption of salivary epithelial formation

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00207.2011

    RvD1 receptor is expressed in salivary epithelium. A : lysates were prepared from Par-C10 cell monolayers and submandibular gland (SMG) cells freshly isolated from C57BL/6 mice and expression of ALX/FPR2 receptor was detected by Western blot analysis.
    Figure Legend Snippet: RvD1 receptor is expressed in salivary epithelium. A : lysates were prepared from Par-C10 cell monolayers and submandibular gland (SMG) cells freshly isolated from C57BL/6 mice and expression of ALX/FPR2 receptor was detected by Western blot analysis.

    Techniques Used: Isolation, Mouse Assay, Expressing, Western Blot

    Proposed signaling mechanisms mediated by RvD1 in Par-C10 cells. RvD1 binds to the ALX/FPR2 receptor and activates phosphatidylinositol 3-kinase (PI3K) and Akt signaling enhancing cell polarity (i.e., ZO-1 apical localization) and migration (i.e., actin
    Figure Legend Snippet: Proposed signaling mechanisms mediated by RvD1 in Par-C10 cells. RvD1 binds to the ALX/FPR2 receptor and activates phosphatidylinositol 3-kinase (PI3K) and Akt signaling enhancing cell polarity (i.e., ZO-1 apical localization) and migration (i.e., actin

    Techniques Used: Migration

    3) Product Images from "BML-111 suppresses TGF-β1-induced lung fibroblast activation in vitro and decreases experimental pulmonary fibrosis in vivo"

    Article Title: BML-111 suppresses TGF-β1-induced lung fibroblast activation in vitro and decreases experimental pulmonary fibrosis in vivo

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3914

    BML-111 decreased TGF-β1-induced NIH3T3 cell α-SMA expression in a dose-dependent manner. (A) NIH3T3 cells express rs1 and (B) FPR2. Cells were pretreated with a vehicle (0.035% ethanol) or BML-111 (1, 10, 100, 200 and 500 nM) for 30 min and then treated with TGF-β1 (5 ng/ml) for 24 h. (C) The expression of α-SMA was assessed using western blotting and (D) quantified. Similar results were obtained from at least 3 sections. Data are expressed as the mean ± standard deviation. # P
    Figure Legend Snippet: BML-111 decreased TGF-β1-induced NIH3T3 cell α-SMA expression in a dose-dependent manner. (A) NIH3T3 cells express rs1 and (B) FPR2. Cells were pretreated with a vehicle (0.035% ethanol) or BML-111 (1, 10, 100, 200 and 500 nM) for 30 min and then treated with TGF-β1 (5 ng/ml) for 24 h. (C) The expression of α-SMA was assessed using western blotting and (D) quantified. Similar results were obtained from at least 3 sections. Data are expressed as the mean ± standard deviation. # P

    Techniques Used: Expressing, Western Blot, Standard Deviation

    4) Product Images from "Lipoxin A4 inhibits immune cell binding to salivary epithelium and vascular endothelium"

    Article Title: Lipoxin A4 inhibits immune cell binding to salivary epithelium and vascular endothelium

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00259.2011

    ALX/formyl peptide receptor 2 (FPR2) is expressed in human submandibular gland (HSG) cells and Jurkat cells. A : protein extracts from Jurkat and HSG cells were subjected to Western blot analysis using rabbit anti-ALX/FPR2 receptor that recognizes a sequence
    Figure Legend Snippet: ALX/formyl peptide receptor 2 (FPR2) is expressed in human submandibular gland (HSG) cells and Jurkat cells. A : protein extracts from Jurkat and HSG cells were subjected to Western blot analysis using rabbit anti-ALX/FPR2 receptor that recognizes a sequence

    Techniques Used: Western Blot, Sequencing

    5) Product Images from "ALX/FPR2 receptor for RvD1 is expressed and functional in salivary glands"

    Article Title: ALX/FPR2 receptor for RvD1 is expressed and functional in salivary glands

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00284.2013

    Proposed signaling mechanisms mediated by RvD1 in mSMG. RvD1 binds to ALX/FPR2 expressed on the membrane of acinar and ductal cells. RvD1 receptors signal through a classical 7-transmembrane G protein-coupled receptor, given that they activate intracellular Ca 2+ ([Ca 2+ ] i ) release. After stimulation, AT-RvD1 activates Erk1/2 and Akt signaling, blocking TNF-α signaling and caspase-3 activity, leading to cell survival. TNFR1, TNF receptor type 1; PI3K, phosphatidylinositol 3-kinase.
    Figure Legend Snippet: Proposed signaling mechanisms mediated by RvD1 in mSMG. RvD1 binds to ALX/FPR2 expressed on the membrane of acinar and ductal cells. RvD1 receptors signal through a classical 7-transmembrane G protein-coupled receptor, given that they activate intracellular Ca 2+ ([Ca 2+ ] i ) release. After stimulation, AT-RvD1 activates Erk1/2 and Akt signaling, blocking TNF-α signaling and caspase-3 activity, leading to cell survival. TNFR1, TNF receptor type 1; PI3K, phosphatidylinositol 3-kinase.

    Techniques Used: Blocking Assay, Activity Assay

    The RvD1 receptor ALX/FPR2 is expressed in human minor salivary glands (hMSG). Frozen hMSG sections without ( A–E ) and with ( F–J ) Sjögren's syndrome (SS) were fixed, and expression of ALX/FPR2 was detected using immunofluorescence microscopy with rabbit anti-ALX/FPR2 (green; A, B, E, F, G , and J ), phalloidin (red; D, E, I , and J ), and propidium iodide nuclear stain (blue; A, C, E, F, H , and J ). All images were obtained and analyzed using a confocal microscope. Arrows and arrowheads indicate acinar and ductal localization of ALX/FPR2, respectively. Scale bars, 50 μm.
    Figure Legend Snippet: The RvD1 receptor ALX/FPR2 is expressed in human minor salivary glands (hMSG). Frozen hMSG sections without ( A–E ) and with ( F–J ) Sjögren's syndrome (SS) were fixed, and expression of ALX/FPR2 was detected using immunofluorescence microscopy with rabbit anti-ALX/FPR2 (green; A, B, E, F, G , and J ), phalloidin (red; D, E, I , and J ), and propidium iodide nuclear stain (blue; A, C, E, F, H , and J ). All images were obtained and analyzed using a confocal microscope. Arrows and arrowheads indicate acinar and ductal localization of ALX/FPR2, respectively. Scale bars, 50 μm.

    Techniques Used: Expressing, Immunofluorescence, Microscopy, Staining

    ALX/FPR2 is functional in mSMG. Cells were plated on 8-well coverglasses treated with Cell-Tak and then stimulated with aspirin-triggered RvD1 (AT-RvD1, 100 ng/ml; A ), carbachol (100 μM; B ), or 1× PBS ( C ). Changes in fluo 4-AM fluorescence intensity were recorded and analyzed using Leica Application Suite Advanced Fluorescence software. Results are representative of ≥3 experiments for all agonists.
    Figure Legend Snippet: ALX/FPR2 is functional in mSMG. Cells were plated on 8-well coverglasses treated with Cell-Tak and then stimulated with aspirin-triggered RvD1 (AT-RvD1, 100 ng/ml; A ), carbachol (100 μM; B ), or 1× PBS ( C ). Changes in fluo 4-AM fluorescence intensity were recorded and analyzed using Leica Application Suite Advanced Fluorescence software. Results are representative of ≥3 experiments for all agonists.

    Techniques Used: Functional Assay, Fluorescence, Software

    The resolvin D1 (RvD1) receptor ALX/FPR2 (lipoxin A 4 /formyl peptide receptor 2) is expressed in mouse submandibular glands (mSMG). Frozen sections of mSMG were fixed, and localization of ALX/FPR2 was determined using immunofluorescence microscopy: rabbit anti-ALX/FPR2 (green; A, B , and E ), phalloidin (red; D and E ), and propidium iodide nuclear stain (blue; A, C , and E ). All images were obtained and analyzed using a confocal microscope. Arrows and arrowheads indicate acinar and ductal localization of the receptor, respectively. Scale bars, 50 μm.
    Figure Legend Snippet: The resolvin D1 (RvD1) receptor ALX/FPR2 (lipoxin A 4 /formyl peptide receptor 2) is expressed in mouse submandibular glands (mSMG). Frozen sections of mSMG were fixed, and localization of ALX/FPR2 was determined using immunofluorescence microscopy: rabbit anti-ALX/FPR2 (green; A, B , and E ), phalloidin (red; D and E ), and propidium iodide nuclear stain (blue; A, C , and E ). All images were obtained and analyzed using a confocal microscope. Arrows and arrowheads indicate acinar and ductal localization of the receptor, respectively. Scale bars, 50 μm.

    Techniques Used: Immunofluorescence, Microscopy, Staining

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    Alomone Labs rabbit anti fpr2
    Distribution of SPM receptors within minor salivary glands from non-SS and SS patients. (A) <t>ALX/FPR2</t> was expressed in mucous acini from both SS and non-SS patients (white arrows), while it was only observed within striated ducts from SS patients (yellow arrow). (B) BLT1 was found at the plasma membrane of both striated ducts (brown arrows) and mucous acini (pink arrows) of both SS and non-SS patients. (C) CMKLR1 was expressed in mucous acini (purple arrows) as well as striated ducts (orange arrows) of both SS and non-SS patients. Red and green arrows indicate inflammatory and endothelial cells, respectively. A representative image from n = 3 per experimental group is shown. Scale bars represent 50 μm.
    Rabbit Anti Fpr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti fpr2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti fpr2 - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

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    Distribution of SPM receptors within minor salivary glands from non-SS and SS patients. (A) ALX/FPR2 was expressed in mucous acini from both SS and non-SS patients (white arrows), while it was only observed within striated ducts from SS patients (yellow arrow). (B) BLT1 was found at the plasma membrane of both striated ducts (brown arrows) and mucous acini (pink arrows) of both SS and non-SS patients. (C) CMKLR1 was expressed in mucous acini (purple arrows) as well as striated ducts (orange arrows) of both SS and non-SS patients. Red and green arrows indicate inflammatory and endothelial cells, respectively. A representative image from n = 3 per experimental group is shown. Scale bars represent 50 μm.

    Journal: Annals of diagnostic pathology

    Article Title: Specialized pro-resolving receptors are expressed in salivary glands with Sjögren’s syndrome

    doi: 10.1016/j.anndiagpath.2021.151865

    Figure Lengend Snippet: Distribution of SPM receptors within minor salivary glands from non-SS and SS patients. (A) ALX/FPR2 was expressed in mucous acini from both SS and non-SS patients (white arrows), while it was only observed within striated ducts from SS patients (yellow arrow). (B) BLT1 was found at the plasma membrane of both striated ducts (brown arrows) and mucous acini (pink arrows) of both SS and non-SS patients. (C) CMKLR1 was expressed in mucous acini (purple arrows) as well as striated ducts (orange arrows) of both SS and non-SS patients. Red and green arrows indicate inflammatory and endothelial cells, respectively. A representative image from n = 3 per experimental group is shown. Scale bars represent 50 μm.

    Article Snippet: Also, rabbit Anti-FPR2 was obtained from Alomone Labs (Jerusalem, IL).

    Techniques:

    RvD1 receptor is expressed in salivary epithelium. A : lysates were prepared from Par-C10 cell monolayers and submandibular gland (SMG) cells freshly isolated from C57BL/6 mice and expression of ALX/FPR2 receptor was detected by Western blot analysis.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Resolvin D1 prevents TNF-?-mediated disruption of salivary epithelial formation

    doi: 10.1152/ajpcell.00207.2011

    Figure Lengend Snippet: RvD1 receptor is expressed in salivary epithelium. A : lysates were prepared from Par-C10 cell monolayers and submandibular gland (SMG) cells freshly isolated from C57BL/6 mice and expression of ALX/FPR2 receptor was detected by Western blot analysis.

    Article Snippet: The cells or sections were incubated overnight at 4°C with rabbit anti-ZO-1 (Invitrogen) or rabbit anti-ALX/FPR2 (Alomone Labs, Jerusalem, Israel) antibody at 1:500 dilution in 5% goat serum containing 10 μM digitonin.

    Techniques: Isolation, Mouse Assay, Expressing, Western Blot

    Proposed signaling mechanisms mediated by RvD1 in Par-C10 cells. RvD1 binds to the ALX/FPR2 receptor and activates phosphatidylinositol 3-kinase (PI3K) and Akt signaling enhancing cell polarity (i.e., ZO-1 apical localization) and migration (i.e., actin

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Resolvin D1 prevents TNF-?-mediated disruption of salivary epithelial formation

    doi: 10.1152/ajpcell.00207.2011

    Figure Lengend Snippet: Proposed signaling mechanisms mediated by RvD1 in Par-C10 cells. RvD1 binds to the ALX/FPR2 receptor and activates phosphatidylinositol 3-kinase (PI3K) and Akt signaling enhancing cell polarity (i.e., ZO-1 apical localization) and migration (i.e., actin

    Article Snippet: The cells or sections were incubated overnight at 4°C with rabbit anti-ZO-1 (Invitrogen) or rabbit anti-ALX/FPR2 (Alomone Labs, Jerusalem, Israel) antibody at 1:500 dilution in 5% goat serum containing 10 μM digitonin.

    Techniques: Migration

    BML-111 decreased TGF-β1-induced NIH3T3 cell α-SMA expression in a dose-dependent manner. (A) NIH3T3 cells express rs1 and (B) FPR2. Cells were pretreated with a vehicle (0.035% ethanol) or BML-111 (1, 10, 100, 200 and 500 nM) for 30 min and then treated with TGF-β1 (5 ng/ml) for 24 h. (C) The expression of α-SMA was assessed using western blotting and (D) quantified. Similar results were obtained from at least 3 sections. Data are expressed as the mean ± standard deviation. # P

    Journal: International Journal of Molecular Medicine

    Article Title: BML-111 suppresses TGF-β1-induced lung fibroblast activation in vitro and decreases experimental pulmonary fibrosis in vivo

    doi: 10.3892/ijmm.2018.3914

    Figure Lengend Snippet: BML-111 decreased TGF-β1-induced NIH3T3 cell α-SMA expression in a dose-dependent manner. (A) NIH3T3 cells express rs1 and (B) FPR2. Cells were pretreated with a vehicle (0.035% ethanol) or BML-111 (1, 10, 100, 200 and 500 nM) for 30 min and then treated with TGF-β1 (5 ng/ml) for 24 h. (C) The expression of α-SMA was assessed using western blotting and (D) quantified. Similar results were obtained from at least 3 sections. Data are expressed as the mean ± standard deviation. # P

    Article Snippet: Following permeabilization, washing and blocking, the cells were incubated with rabbit anti-FPR2 anti-bodies (1:100; cat. no. AFR-002; Alomone Labs, Jerusalem, Israel) at 4°C overnight and then washed and incubated with a fluorescent-labeled secondary antibody (fluorescein isothiocyanate-labelled goat anti-rabbit immunoglobulin G; 1:50; cat. no. AS1110; Aspen Biological, Wuhan, China) for 45 min at 37°C.

    Techniques: Expressing, Western Blot, Standard Deviation

    ALX/formyl peptide receptor 2 (FPR2) is expressed in human submandibular gland (HSG) cells and Jurkat cells. A : protein extracts from Jurkat and HSG cells were subjected to Western blot analysis using rabbit anti-ALX/FPR2 receptor that recognizes a sequence

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Lipoxin A4 inhibits immune cell binding to salivary epithelium and vascular endothelium

    doi: 10.1152/ajpcell.00259.2011

    Figure Lengend Snippet: ALX/formyl peptide receptor 2 (FPR2) is expressed in human submandibular gland (HSG) cells and Jurkat cells. A : protein extracts from Jurkat and HSG cells were subjected to Western blot analysis using rabbit anti-ALX/FPR2 receptor that recognizes a sequence

    Article Snippet: The cells were incubated overnight at 4°C with rabbit anti-CysLT1 receptor (Cayman Chemicals) or rabbit anti-ALX/FPR2 receptor (Alomone Labs) antibody at 1:200 dilution in 5% goat serum containing 10 μM digitonin.

    Techniques: Western Blot, Sequencing