kcnk10  (Alomone Labs)


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    Alomone Labs kcnk10
    Kcnk10, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcnk10/product/Alomone Labs
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    kcnk10 - by Bioz Stars, 2022-12
    91/100 stars

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    Alomone Labs polyclonal anti trek 2
    Upregulation of <t>TREK-2</t> by the GABA receptor agonists baclofen and muscimol in B35 cells. ( A ) The alteration of TREK-1, TREK-2, and TRAAK mRNA expression levels via treatment with baclofen or muscimol. B35 cells were treated with the chemicals (100 μM) for 24 h. ( B ) The TREK-2 protein expression level affected by GABAR modulators. Each bar represents the mean ± SD of four independent experiments. * p
    Polyclonal Anti Trek 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti trek 2/product/Alomone Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    polyclonal anti trek 2 - by Bioz Stars, 2022-12
    93/100 stars
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    Upregulation of TREK-2 by the GABA receptor agonists baclofen and muscimol in B35 cells. ( A ) The alteration of TREK-1, TREK-2, and TRAAK mRNA expression levels via treatment with baclofen or muscimol. B35 cells were treated with the chemicals (100 μM) for 24 h. ( B ) The TREK-2 protein expression level affected by GABAR modulators. Each bar represents the mean ± SD of four independent experiments. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Muscimol Directly Activates the TREK-2 Channel Expressed in GABAergic Neurons through Its N-Terminus

    doi: 10.3390/ijms22179320

    Figure Lengend Snippet: Upregulation of TREK-2 by the GABA receptor agonists baclofen and muscimol in B35 cells. ( A ) The alteration of TREK-1, TREK-2, and TRAAK mRNA expression levels via treatment with baclofen or muscimol. B35 cells were treated with the chemicals (100 μM) for 24 h. ( B ) The TREK-2 protein expression level affected by GABAR modulators. Each bar represents the mean ± SD of four independent experiments. * p

    Article Snippet: Membranes were blocked with 5% (w /v ) fat-free dry milk in tris-buffered saline with Tween-20 (TBST; 20 mM Tris HCl (pH 8.0), 137 mM NaCl, and 0.2% Tween-20) at room temperature for 60 min and then incubated with polyclonal anti-TREK-2 (1:500 dilution, Alomone Labs) and monoclonal anti-β-actin antibodies (1:5000 dilution) at 4 °C overnight.

    Techniques: Expressing

    Expression of TREK/TRAAK in GABAergic neurons. ( A ) Localization of TREK-1, TREK-2, and TRAAK proteins in B35 cells. Cells were immunostained with anti-TREK-1, -TREK-2, or -TRAAK antibodies, and subjected to propidium iodide (PI) staining for nuclear staining. The merged image was output as an overlay of green fluorescence (FITC) and red PI-stain images. In the negative control (NC) group, the primary antibody was omitted. ( B ) The expression pattern of GABAergic neurons in the brain obtained from the GAD67-EGFP transgenic mice. The upper panel and lower panel present coronal section and sagittal section images, respectively. In the coronal and sagittal section images, a , b , and c represent the cortex, hippocampus, and medulla, respectively. ( C ) Co-localized TREK/TRAAK signals and GAD67-GFP signals in the medulla. GABAergic neurons expressed EGFP in green. The indicated antigen was immunostained red using CY3. EGFP and CY3 signals were merged in yellow (merge). An expanded view of the white box in the merged column is presented on the right side. Scale bar, 40 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Muscimol Directly Activates the TREK-2 Channel Expressed in GABAergic Neurons through Its N-Terminus

    doi: 10.3390/ijms22179320

    Figure Lengend Snippet: Expression of TREK/TRAAK in GABAergic neurons. ( A ) Localization of TREK-1, TREK-2, and TRAAK proteins in B35 cells. Cells were immunostained with anti-TREK-1, -TREK-2, or -TRAAK antibodies, and subjected to propidium iodide (PI) staining for nuclear staining. The merged image was output as an overlay of green fluorescence (FITC) and red PI-stain images. In the negative control (NC) group, the primary antibody was omitted. ( B ) The expression pattern of GABAergic neurons in the brain obtained from the GAD67-EGFP transgenic mice. The upper panel and lower panel present coronal section and sagittal section images, respectively. In the coronal and sagittal section images, a , b , and c represent the cortex, hippocampus, and medulla, respectively. ( C ) Co-localized TREK/TRAAK signals and GAD67-GFP signals in the medulla. GABAergic neurons expressed EGFP in green. The indicated antigen was immunostained red using CY3. EGFP and CY3 signals were merged in yellow (merge). An expanded view of the white box in the merged column is presented on the right side. Scale bar, 40 μm.

    Article Snippet: Membranes were blocked with 5% (w /v ) fat-free dry milk in tris-buffered saline with Tween-20 (TBST; 20 mM Tris HCl (pH 8.0), 137 mM NaCl, and 0.2% Tween-20) at room temperature for 60 min and then incubated with polyclonal anti-TREK-2 (1:500 dilution, Alomone Labs) and monoclonal anti-β-actin antibodies (1:5000 dilution) at 4 °C overnight.

    Techniques: Expressing, Staining, Fluorescence, Negative Control, Transgenic Assay, Mouse Assay

    Expression of the K 2P channels in B35 cells. ( A ) A photomicrograph of B35 cells cultured in a 24-well culture dish. Scale bar, 50 μm. ( B ) Expression of GABA A and GABA B receptors. ( C ) Expression of TREK-1, TREK-2, TRAAK, TASK-1, and TASK-3 corresponding to the expected sizes of 361 bp, 493 bp, 445 bp, 702 bp, and 517-bp, respectively. The first, fourth, and tenth lanes presented 100 bp DNA ladders. RT + and RT – indicated, respectively, reactions with and without reverse transcriptase. The arrowheads indicate the GABA B receptor and TRAAK bands. ( D ) Relative mRNA abundance of the K 2P channels. The mRNA expression levels of TASK-1, TASK-3, TREK-1, TREK-2, and TRAAK were quantified by real-time PCR. The mRNA expression level was normalized to GAPDH. Each bar represents the mean ± SD of four independent experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: Muscimol Directly Activates the TREK-2 Channel Expressed in GABAergic Neurons through Its N-Terminus

    doi: 10.3390/ijms22179320

    Figure Lengend Snippet: Expression of the K 2P channels in B35 cells. ( A ) A photomicrograph of B35 cells cultured in a 24-well culture dish. Scale bar, 50 μm. ( B ) Expression of GABA A and GABA B receptors. ( C ) Expression of TREK-1, TREK-2, TRAAK, TASK-1, and TASK-3 corresponding to the expected sizes of 361 bp, 493 bp, 445 bp, 702 bp, and 517-bp, respectively. The first, fourth, and tenth lanes presented 100 bp DNA ladders. RT + and RT – indicated, respectively, reactions with and without reverse transcriptase. The arrowheads indicate the GABA B receptor and TRAAK bands. ( D ) Relative mRNA abundance of the K 2P channels. The mRNA expression levels of TASK-1, TASK-3, TREK-1, TREK-2, and TRAAK were quantified by real-time PCR. The mRNA expression level was normalized to GAPDH. Each bar represents the mean ± SD of four independent experiments.

    Article Snippet: Membranes were blocked with 5% (w /v ) fat-free dry milk in tris-buffered saline with Tween-20 (TBST; 20 mM Tris HCl (pH 8.0), 137 mM NaCl, and 0.2% Tween-20) at room temperature for 60 min and then incubated with polyclonal anti-TREK-2 (1:500 dilution, Alomone Labs) and monoclonal anti-β-actin antibodies (1:5000 dilution) at 4 °C overnight.

    Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction

    TREK-2 activation by muscimol. ( A ) Background K + currents recorded in B35 cells. The currents were recorded in the presence of 4-aminopyridine (4-AP, 1 mM), BaCl 2 (1 mM), and TEA (1 mM) to rule out the involvement of other K + channels. ( B ) Muscimol-induced activation of TREK-2 currents in HEK-293 cells. TREK-2 was transfected into HEK293 cells. ( C ) Effect of muscimol on TREK-2 activation under different patch configurations. The muscimol was applied to the bath solution at a rate of 6 mL/min. The number of events analyzed for comparison of NPo was 2000. Channel activity was analyzed within 1 min after the application of muscimol at -60 mV. ( D ) The action sites of muscimol on the TREK-2 wild-type and N-terminal and C-terminal deletion (ΔN and ΔC) mutants. The whole-cell current was recorded in response to a voltage ramp (−120 to +60 mV; 1 s duration) from a holding potential of −80 mV. The currents measured at +60 mV were analyzed. ( E ) Binding assay of TREK-2 and muscimol. TREK-2 in the pcDNA3-EGFP vector was transfected into HEK-293 cells, and the BODIPY ® TMR-X muscimol conjugate was stained. Scale bar, 30 μm. Each bar ( A – D ) represents the mean ± SD of three independent experiments ( n = 12). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Muscimol Directly Activates the TREK-2 Channel Expressed in GABAergic Neurons through Its N-Terminus

    doi: 10.3390/ijms22179320

    Figure Lengend Snippet: TREK-2 activation by muscimol. ( A ) Background K + currents recorded in B35 cells. The currents were recorded in the presence of 4-aminopyridine (4-AP, 1 mM), BaCl 2 (1 mM), and TEA (1 mM) to rule out the involvement of other K + channels. ( B ) Muscimol-induced activation of TREK-2 currents in HEK-293 cells. TREK-2 was transfected into HEK293 cells. ( C ) Effect of muscimol on TREK-2 activation under different patch configurations. The muscimol was applied to the bath solution at a rate of 6 mL/min. The number of events analyzed for comparison of NPo was 2000. Channel activity was analyzed within 1 min after the application of muscimol at -60 mV. ( D ) The action sites of muscimol on the TREK-2 wild-type and N-terminal and C-terminal deletion (ΔN and ΔC) mutants. The whole-cell current was recorded in response to a voltage ramp (−120 to +60 mV; 1 s duration) from a holding potential of −80 mV. The currents measured at +60 mV were analyzed. ( E ) Binding assay of TREK-2 and muscimol. TREK-2 in the pcDNA3-EGFP vector was transfected into HEK-293 cells, and the BODIPY ® TMR-X muscimol conjugate was stained. Scale bar, 30 μm. Each bar ( A – D ) represents the mean ± SD of three independent experiments ( n = 12). * p

    Article Snippet: Membranes were blocked with 5% (w /v ) fat-free dry milk in tris-buffered saline with Tween-20 (TBST; 20 mM Tris HCl (pH 8.0), 137 mM NaCl, and 0.2% Tween-20) at room temperature for 60 min and then incubated with polyclonal anti-TREK-2 (1:500 dilution, Alomone Labs) and monoclonal anti-β-actin antibodies (1:5000 dilution) at 4 °C overnight.

    Techniques: Activation Assay, Transfection, Activity Assay, Binding Assay, Plasmid Preparation, Staining