Kcnq3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Protein kinase CK2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G"
Article Title: Protein kinase CK2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G
Journal: The Journal of Cell Biology
Figure Legend Snippet: Impact of K v 2.1-Na v 1.2 and of phosphorylation-deficient K v 2.1-Na v 1.2 constructs on the accumulation of sodium channels (Na v 1) and KCNQ2/KCNQ3 potassium channels at the AIS of cultured hippocampal neurons. (A, top and middle) K v 2.1-Na v 1.2 expression perturbed Na v 1 accumulation at the AIS, unlike the phosphorylation-deficient K v 2.1-Na v 1.2 4SA mutant. Hippocampal neurons were transfected with either K v 2.1-Na v 1.2 or phosphorylation-deficient K v 2.1-Na v 1.2 mutants. Then cells were stained for myc (gray), ankyrin G (red), and sodium channels (green). (B) Quantification of Na v 1 and ankyrin G staining intensity in untransfected cells (A, open arrowheads) and in transfected cells (closed arrowheads). (A, bottom) K v 2.1-Na v 1.2 expression did not perturb KCNQ3 accumulation at the AIS. Cells transfected with K v 2.1-Na v 1.2 were subsequently stained for myc (gray), ankyrin G (red), and KCNQ3 potassium channels (green). (C) Quantification of KCNQ3 and ankyrin G staining intensity. Fluorescence intensity measured in transfected cells, identified by myc staining, was normalized by taking as 100% the staining intensity measured in nontransfected cells (arrowheads). Numbers at the base of the bars denote the number of quantified cells. Error bars indicate mean ± SEM. Mann-Whitney test: *, P
Techniques Used: Construct, Cell Culture, Expressing, Mutagenesis, Transfection, Staining, Fluorescence, MANN-WHITNEY