trpm4 (Alomone Labs)


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Trpm4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trpm4/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "Novel regulatory mechanism in human urinary bladder: central role of transient receptor potential melastatin 4 channels in detrusor smooth muscle function"
Article Title: Novel regulatory mechanism in human urinary bladder: central role of transient receptor potential melastatin 4 channels in detrusor smooth muscle function
Journal: American Journal of Physiology - Cell Physiology
doi: 10.1152/ajpcell.00270.2015

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol attenuates spontaneous phasic and tonic contractions of human DSM isolated strips. A : representative myograph recording demonstrating inhibition of spontaneous phasic contractions and tone of a human DSM isolated strip by 30 μM 9-phenanthrol. B : summary data illustrating decrease in spontaneous phasic contraction amplitude, muscle force integral, frequency, duration, and tone of human DSM strips by 30 μM 9-phenanthrol ( n = 11, N = 5). *** P
Techniques Used: Inhibition, Isolation, Stripping Membranes

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol hyperpolarizes the resting membrane potential (RMP) in human DSM cells. A : representative current-clamp recording illustrating hyperpolarizing effects of 30 μM 9-phenanthrol on RMP in a human DSM cell. B : summary data illustrating hyperpolarizing effects of 30 μM 9-phenanthrol on the RMP in human DSM cells ( n = 4, N = 3). * P
Techniques Used: Inhibition

Figure Legend Snippet: Western blot, immunohistochemical, and immunocytochemical detections of TRPM4 channel protein in human DSM tissue and DSM single cells. A : Western blot showing TRPM4 channel protein expression in human DSM tissue. Arrow indicates ∼134-kDa band, consistent with expected molecular mass of TRPM4 channel protein. Lack of an immunoreactive band in the presence of competing peptide (CP) confirmed specificity of the primary antibody. HEK-293 cell lysate was used as a positive control. B : confocal images showing immunohistochemical detection of TRPM4 channel protein expression in human DSM tissue. Red staining ( bottom left ) indicates TRPM4 channels; blue staining indicates cell nuclei ( top left ); green staining indicates α-smooth muscle actin (α-SMA, top right ); merged image ( bottom right ) illustrates overlap of all 3 images. C : confocal images illustrating immunocytochemical detection of TRPM4 channel protein expression in isolated human DSM cells. Red staining ( bottom left ) indicates TRPM4 channels; blue staining indicates cell nucleus ( top left ); green staining indicates α-SMA ( top right ); merged image ( bottom right ) illustrates overlap of all 3 images. Results were verified in 4 separate experiments using DSM whole tissue or multiple DSM cells isolated from 4 patients. D and E : immunohistochemistry and immunocytochemistry experiments, respectively. In control experiments, no staining was visible after absorption of the primary antibody with a competing peptide (+CP control). Merged image ( bottom right ) illustrates overlap of all 3 images.
Techniques Used: Western Blot, Immunohistochemistry, Expressing, Positive Control, Staining, Isolation, Immunocytochemistry

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol decreases the amplitude of 3.5- to 50-Hz EFS-induced contractions of human DSM isolated strips. A : representative recording of EFS-induced contractions (stimulation frequency = 3.5–50 Hz) in the absence of 9-phenanthrol (control) and 10 min after application of 30 μM 9-phenanthrol. B : frequency-response curves in the presence or absence of 30 μM 9-phenanthrol illustrating a decrease in amplitude of EFS-induced contractions of human DSM isolated strips ( n = 22, N = 16). ** P
Techniques Used: Inhibition, Isolation

Figure Legend Snippet: Transient receptor potential melastatin 4 (TRPM4) channel mRNA expression in human detrusor smooth muscle (DSM) whole tissue and native freshly isolated human DSM single cells. Gel electrophoresis imaging illustrates RT-PCR detection of TRPM4 channel mRNA transcripts (196 bp) in DSM whole tissue and DSM single cells ( n = 4, N = 4). Human brain and prostate samples were used as positive controls (+). No products were observed in the negative control (−RT), in which reverse transcriptase (RT) was not added to the reaction.
Techniques Used: Expressing, Isolation, Nucleic Acid Electrophoresis, Imaging, Reverse Transcription Polymerase Chain Reaction, Negative Control

Figure Legend Snippet: Inhibition of transient inward cationic currents (TICCs) by the TRPM4 channel-selective inhibitor 9-phenanthrol in freshly isolated human DSM cells. A : original recording illustrating the inhibitory effect of 30 μM 9-phenanthrol on TICC activity in a human DSM single cell recorded at −70 mV. B : summary data illustrating inhibitory effects of 30 μM 9-phenanthrol on TICCs, analyzed as total open channel probability ( NP o ) before and after application of 30 μM 9-phenanthrol ( n = 15, N = 9). * P
Techniques Used: Inhibition, Isolation, Activity Assay

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol attenuates voltage step-induced whole cell currents in human DSM cells. A : representative recordings illustrate the inhibitory effect of 30 μM 9-phenanthrol on the amplitude of the voltage step-induced whole cell current in human DSM cell. Inhibitory effect of 9-phenanthrol was reversed after washout of the human DSM cell with fresh 9-phenanthrol-free bath solution. B : summary data of current-voltage relationships in the absence (control), in the presence, and after washout of 30 μM 9-phenanthrol ( n = 7, N = 7). * P
Techniques Used: Inhibition

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol significantly reduces carbachol-induced phasic and tonic contractions of human DSM isolated strips. A : representative myograph recording obtained from a human DSM strip depicting the inhibitory effect of 30 μM 9-phenanthrol on 0.1 μM carbachol-induced phasic contractions. B : summary data illustrating inhibitory effects of 30 μM 9-phenanthrol on amplitude, muscle force integral, frequency, duration, and tone of carbachol-induced contractions of human DSM isolated strips ( n = 17, N = 5). *** P
Techniques Used: Inhibition, Isolation, Stripping Membranes
2) Product Images from "Cholesterol Stimulates the Transient Receptor Potential Melastatin 4 Channel in mpkCCDc14 Cells"
Article Title: Cholesterol Stimulates the Transient Receptor Potential Melastatin 4 Channel in mpkCCDc14 Cells
Journal: Frontiers in Pharmacology
doi: 10.3389/fphar.2021.627875

Figure Legend Snippet: Application of MβCD to the cytoplasmic bath decreases TRPM4 channel activity which is reversed by exogenous cholesterol. (A) A representative single channel recording from an inside-out patch shows TRPM4 activity before and after replacement of control cytoplasmic bath solution first with a solution containing 0.5 mM MβCD and then with a solution containing 30 μg/ml cholesterol. “C” indicates channel at the closed state. “O” indicates single-level openings. “I, II, and III” are zoom-ins of the single-channel recording. (B) Summary plots of TRPM4 channel P O under each indicted condition. n = 5 paired experiments, ** P
Techniques Used: Activity Assay

Figure Legend Snippet: Treatment of cells with cholesterol increases, but with lovastatin decreases, TRPM4 channel activity by regulating its sensitivity to Ca 2+ in mpkCCD c14 Cells. (A) Representative single channel recording from inside-out patches exposed the patch membrane to the bath containing different concentrations of free Ca 2+ . The cells were treated with 30 μg/ml cholesterol for 48 hrs. From top to bottom: 1 μM, 10 μM, 200 μM, 1 mM, and 5 mM free bath Ca 2+ . “C” indicates channel at the closed state; “O” indicates single-level openings. (B) Representative single channel recording from inside-out patches exposed the patch membrane to the bath containing different concentrations of free Ca 2+ . The cells were treated with 5 μM lovastatin for 48 hrs. From top to bottom: 10 μM, 200 μM, 1 mM, 5 mM and 10 mM free bath Ca 2+ . “C” indicates channel at the closed state; “O” indicates single-level openings. (C) The effect of membrane cholesterol on Ca 2+ -dependence of channel opening. Channel P O was plotted as a function of free Ca 2+ concentration in the bath. P o values are shown for patches either with exogenous cholesterol treatment (black line) or lovastatin treatment (red line). n = 4–7 cells for different data points.
Techniques Used: Activity Assay, Concentration Assay

Figure Legend Snippet: PI(4, 5)P 2 stimulates TRPM4 via a physical interaction. (A) A representative single channel recording shows that diC8-PI(4,5)P 2 significantly increased TRPM4 activity. (B) Summary plots of TRPM4 channel P O under each indicated conditions. n = 5 paired experiments. ** P
Techniques Used: Activity Assay

Figure Legend Snippet: Cholesterol stimulates TRPM4 via a PI(4, 5)P 2 -dependent mechanism. (A) A representative single channel recording shows that treatment of mpkCCD c14 cells with 20 nM wortmannin had no effect on cholesterol-induced TRPM4 channel activity. (B) Summary plots of TRPM4 channel P O under each indicated conditions. n = 6 paired experiments, ** P
Techniques Used: Activity Assay

Figure Legend Snippet: TRPM4 channels are mainly located in lipid rafts. (A) Representative confocal microscopy images indicate that majority of TRPM4 (green) was co-localized with cholera toxin B (red) in the apical membrane; white rectangular box indicate zoomed-in areas shown in the Zoom-in panels. Data represent five individual experiments showing consistent results (B) Majority of TRPM4 was detected in low-density regions in sucrose gradient experiments; Caveolin-1 was used as a control protein that is known to be located in lipid rafts. Data represent three individual experiments showing consistent results.
Techniques Used: Confocal Microscopy

Figure Legend Snippet: Treatment with cholesterol or lovastatin does not alter expression levels of TRPM4 in mpkCCDc14 Cells. (A) Representative confocal microscopy images of mpkCCDc14 cells stained with TRPM4 antibody under each indicated conditions. (B) Summary plots of fluorescence intensity of TRPM4. Data are from 24 cells in four sets of separate experiments. (C) Representative Western blots from cell-surface biotinylated and the total proteins of TRPM4 protein. (D) Summary plots of relative expression of TRPM4. Cells were either under control conditions or treated with 30 μg/ml cholesterol alone, 30 μg/ml cholesterol plus 5 μM lovastatin, or 5 μM lovastatin alone for 48 hrs, respectively. n = 5. (E) Representative single channel recording from inside-out patches exposed the patch membrane to the bath containing 5 mM CaCl 2 , followed by a bath solution with 10 mM EGTA and no calcium. Cells were either under control conditions or treated with 30 μg/ml cholesterol, or 5 μM lovastatin for 48 hrs, respectively. (F) Summary plots of the number of active channels in the patches under each indicated condition. n = 5 for control cells, n = 5 for cells treated with cholesterol, n = 4 for cells treated with lovastatin.
Techniques Used: Expressing, Confocal Microscopy, Staining, Fluorescence, Western Blot
3) Product Images from "Novel regulatory mechanism in human urinary bladder: central role of transient receptor potential melastatin 4 channels in detrusor smooth muscle function"
Article Title: Novel regulatory mechanism in human urinary bladder: central role of transient receptor potential melastatin 4 channels in detrusor smooth muscle function
Journal: American Journal of Physiology - Cell Physiology
doi: 10.1152/ajpcell.00270.2015

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol attenuates spontaneous phasic and tonic contractions of human DSM isolated strips. A : representative myograph recording demonstrating inhibition of spontaneous phasic contractions and tone of a human DSM isolated strip by 30 μM 9-phenanthrol. B : summary data illustrating decrease in spontaneous phasic contraction amplitude, muscle force integral, frequency, duration, and tone of human DSM strips by 30 μM 9-phenanthrol ( n = 11, N = 5). *** P
Techniques Used: Inhibition, Isolation, Stripping Membranes

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol hyperpolarizes the resting membrane potential (RMP) in human DSM cells. A : representative current-clamp recording illustrating hyperpolarizing effects of 30 μM 9-phenanthrol on RMP in a human DSM cell. B : summary data illustrating hyperpolarizing effects of 30 μM 9-phenanthrol on the RMP in human DSM cells ( n = 4, N = 3). * P
Techniques Used: Inhibition

Figure Legend Snippet: Western blot, immunohistochemical, and immunocytochemical detections of TRPM4 channel protein in human DSM tissue and DSM single cells. A : Western blot showing TRPM4 channel protein expression in human DSM tissue. Arrow indicates ∼134-kDa band, consistent with expected molecular mass of TRPM4 channel protein. Lack of an immunoreactive band in the presence of competing peptide (CP) confirmed specificity of the primary antibody. HEK-293 cell lysate was used as a positive control. B : confocal images showing immunohistochemical detection of TRPM4 channel protein expression in human DSM tissue. Red staining ( bottom left ) indicates TRPM4 channels; blue staining indicates cell nuclei ( top left ); green staining indicates α-smooth muscle actin (α-SMA, top right ); merged image ( bottom right ) illustrates overlap of all 3 images. C : confocal images illustrating immunocytochemical detection of TRPM4 channel protein expression in isolated human DSM cells. Red staining ( bottom left ) indicates TRPM4 channels; blue staining indicates cell nucleus ( top left ); green staining indicates α-SMA ( top right ); merged image ( bottom right ) illustrates overlap of all 3 images. Results were verified in 4 separate experiments using DSM whole tissue or multiple DSM cells isolated from 4 patients. D and E : immunohistochemistry and immunocytochemistry experiments, respectively. In control experiments, no staining was visible after absorption of the primary antibody with a competing peptide (+CP control). Merged image ( bottom right ) illustrates overlap of all 3 images.
Techniques Used: Western Blot, Immunohistochemistry, Expressing, Positive Control, Staining, Isolation, Immunocytochemistry

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol decreases the amplitude of 3.5- to 50-Hz EFS-induced contractions of human DSM isolated strips. A : representative recording of EFS-induced contractions (stimulation frequency = 3.5–50 Hz) in the absence of 9-phenanthrol (control) and 10 min after application of 30 μM 9-phenanthrol. B : frequency-response curves in the presence or absence of 30 μM 9-phenanthrol illustrating a decrease in amplitude of EFS-induced contractions of human DSM isolated strips ( n = 22, N = 16). ** P
Techniques Used: Inhibition, Isolation

Figure Legend Snippet: Transient receptor potential melastatin 4 (TRPM4) channel mRNA expression in human detrusor smooth muscle (DSM) whole tissue and native freshly isolated human DSM single cells. Gel electrophoresis imaging illustrates RT-PCR detection of TRPM4 channel mRNA transcripts (196 bp) in DSM whole tissue and DSM single cells ( n = 4, N = 4). Human brain and prostate samples were used as positive controls (+). No products were observed in the negative control (−RT), in which reverse transcriptase (RT) was not added to the reaction.
Techniques Used: Expressing, Isolation, Nucleic Acid Electrophoresis, Imaging, Reverse Transcription Polymerase Chain Reaction, Negative Control

Figure Legend Snippet: Inhibition of transient inward cationic currents (TICCs) by the TRPM4 channel-selective inhibitor 9-phenanthrol in freshly isolated human DSM cells. A : original recording illustrating the inhibitory effect of 30 μM 9-phenanthrol on TICC activity in a human DSM single cell recorded at −70 mV. B : summary data illustrating inhibitory effects of 30 μM 9-phenanthrol on TICCs, analyzed as total open channel probability ( NP o ) before and after application of 30 μM 9-phenanthrol ( n = 15, N = 9). * P
Techniques Used: Inhibition, Isolation, Activity Assay

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol attenuates voltage step-induced whole cell currents in human DSM cells. A : representative recordings illustrate the inhibitory effect of 30 μM 9-phenanthrol on the amplitude of the voltage step-induced whole cell current in human DSM cell. Inhibitory effect of 9-phenanthrol was reversed after washout of the human DSM cell with fresh 9-phenanthrol-free bath solution. B : summary data of current-voltage relationships in the absence (control), in the presence, and after washout of 30 μM 9-phenanthrol ( n = 7, N = 7). * P
Techniques Used: Inhibition

Figure Legend Snippet: Inhibition of TRPM4 channels with 9-phenanthrol significantly reduces carbachol-induced phasic and tonic contractions of human DSM isolated strips. A : representative myograph recording obtained from a human DSM strip depicting the inhibitory effect of 30 μM 9-phenanthrol on 0.1 μM carbachol-induced phasic contractions. B : summary data illustrating inhibitory effects of 30 μM 9-phenanthrol on amplitude, muscle force integral, frequency, duration, and tone of carbachol-induced contractions of human DSM isolated strips ( n = 17, N = 5). *** P
Techniques Used: Inhibition, Isolation, Stripping Membranes