trpm4  (Alomone Labs)


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    Structured Review

    Alomone Labs trpm4
    <t>TRPM4</t> does not contribute to synaptic responses. ( a – d ) Effect of 9-phenanthrol (9-P, 30 µM) on EPSPs recorded from CA1 pyramidal neurons in acute slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( a , c ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in 9-P (black). ( b , d ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in 9-P (n = 5, individual responses, mean ± SEM) ( e – h ) Effect of glibenclamide (Glib. 20 µM) on EPSPs recorded from CA1 pyramidal neurons in slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( e , g ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in glibenclamide (black). ( f , h ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in glibenclamide (n = 6, individual responses, mean ± SEM). Non-normalized data and Cumming’s plots are shown in Supplemental Fig. .
    Trpm4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpm4/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpm4 - by Bioz Stars, 2023-06
    93/100 stars

    Images

    1) Product Images from "A glibenclamide-sensitive TRPM4-mediated component of CA1 excitatory postsynaptic potentials appears in experimental autoimmune encephalomyelitis"

    Article Title: A glibenclamide-sensitive TRPM4-mediated component of CA1 excitatory postsynaptic potentials appears in experimental autoimmune encephalomyelitis

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-09875-6

    TRPM4 does not contribute to synaptic responses. ( a – d ) Effect of 9-phenanthrol (9-P, 30 µM) on EPSPs recorded from CA1 pyramidal neurons in acute slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( a , c ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in 9-P (black). ( b , d ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in 9-P (n = 5, individual responses, mean ± SEM) ( e – h ) Effect of glibenclamide (Glib. 20 µM) on EPSPs recorded from CA1 pyramidal neurons in slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( e , g ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in glibenclamide (black). ( f , h ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in glibenclamide (n = 6, individual responses, mean ± SEM). Non-normalized data and Cumming’s plots are shown in Supplemental Fig. .
    Figure Legend Snippet: TRPM4 does not contribute to synaptic responses. ( a – d ) Effect of 9-phenanthrol (9-P, 30 µM) on EPSPs recorded from CA1 pyramidal neurons in acute slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( a , c ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in 9-P (black). ( b , d ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in 9-P (n = 5, individual responses, mean ± SEM) ( e – h ) Effect of glibenclamide (Glib. 20 µM) on EPSPs recorded from CA1 pyramidal neurons in slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( e , g ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in glibenclamide (black). ( f , h ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in glibenclamide (n = 6, individual responses, mean ± SEM). Non-normalized data and Cumming’s plots are shown in Supplemental Fig. .

    Techniques Used: Knock-Out

    The induction and acute phase of EAE was not different in Trpm4 –/– and Trpm4 + / + littermates. ( a ) Daily clinical scores and ( b ) body weight of Trpm4 –/– (orange circles, n = 6 mice) and Trpm4 + / + (blue circles, n = 6 mice) littermates recorded daily after induction of experimental autoimmune encephalomyelitis (EAE) on day 0 (mean ± SEM). ( c ) EAE clinical progression of individual mice and score at time of sacrifice for electrophysiology (X’s). ( d ) Cell resistance was calculated from 200 ms–10 pA current injections in drug-free ACSF. Cell resistance is negatively correlated with the final clinical score in Trpm4 + / + mice (Pearson’s r = − 0.77, p = 0.0035) but not in Trpm4 –/– mice (Pearson’s r = − 0.2, p = 0.58).
    Figure Legend Snippet: The induction and acute phase of EAE was not different in Trpm4 –/– and Trpm4 + / + littermates. ( a ) Daily clinical scores and ( b ) body weight of Trpm4 –/– (orange circles, n = 6 mice) and Trpm4 + / + (blue circles, n = 6 mice) littermates recorded daily after induction of experimental autoimmune encephalomyelitis (EAE) on day 0 (mean ± SEM). ( c ) EAE clinical progression of individual mice and score at time of sacrifice for electrophysiology (X’s). ( d ) Cell resistance was calculated from 200 ms–10 pA current injections in drug-free ACSF. Cell resistance is negatively correlated with the final clinical score in Trpm4 + / + mice (Pearson’s r = − 0.77, p = 0.0035) but not in Trpm4 –/– mice (Pearson’s r = − 0.2, p = 0.58).

    Techniques Used:

    Only cell resistance significantly varies between genotype or health status at baseline.
    Figure Legend Snippet: Only cell resistance significantly varies between genotype or health status at baseline.

    Techniques Used:

    Glibenclamide, not 9-phenanthrol, reduces EPSP peak amplitude in CA1 pyramidal neurons in EAE mice. ( a ) EPSPs recorded from a representative CA1 pyramidal neuron in response to stimulation of stratum radiatum in an acute slice from a Trpm4 + / + mouse prepared during the acute response to EAE. After establishing a stable baseline (blue trace), 9-phenanthrol (30 μM, 9-P) was washed into the bath for 20 min (black trace). ( b ) Normalized average EPSP peak and area under the curve (AUC) at baseline (average 5 min) and after wash-in of 9-phenanthrol (average 15–20 min) (n = 4, individual experiments, mean ± SEM). ( c ) As in a but black trace is after wash-in of glibenclamide (20 µM, Glib). ( d ) As in B but before and after washing in glibenclamide (n = 8, individual experiments, mean ± SEM, *p = 0.02 Wilcoxon paired test). ( e ) As in C but example EPSPs were recorded before (orange trace) and after washing in glibenclamide (black trace) onto a CA1 neuron in a slice made from a Trpm4 -/- EAE littermate. ( f ) As in D but from Trpm4 -/- littermates (n = 7, individual experiments, mean ± SEM). Non-normalized data and Cumming’s plots are in Supplemental Fig. .
    Figure Legend Snippet: Glibenclamide, not 9-phenanthrol, reduces EPSP peak amplitude in CA1 pyramidal neurons in EAE mice. ( a ) EPSPs recorded from a representative CA1 pyramidal neuron in response to stimulation of stratum radiatum in an acute slice from a Trpm4 + / + mouse prepared during the acute response to EAE. After establishing a stable baseline (blue trace), 9-phenanthrol (30 μM, 9-P) was washed into the bath for 20 min (black trace). ( b ) Normalized average EPSP peak and area under the curve (AUC) at baseline (average 5 min) and after wash-in of 9-phenanthrol (average 15–20 min) (n = 4, individual experiments, mean ± SEM). ( c ) As in a but black trace is after wash-in of glibenclamide (20 µM, Glib). ( d ) As in B but before and after washing in glibenclamide (n = 8, individual experiments, mean ± SEM, *p = 0.02 Wilcoxon paired test). ( e ) As in C but example EPSPs were recorded before (orange trace) and after washing in glibenclamide (black trace) onto a CA1 neuron in a slice made from a Trpm4 -/- EAE littermate. ( f ) As in D but from Trpm4 -/- littermates (n = 7, individual experiments, mean ± SEM). Non-normalized data and Cumming’s plots are in Supplemental Fig. .

    Techniques Used:

    TRPM4 mRNA expression is unchanged in cortical neurons from healthy and EAE mice. Cortical nuclei from healthy and EAE-induced mice were sorted by NeuN immunoreactivity. TRPM4 expression levels were assessed by quantitative PCR. n = 5; unpaired 2-tailed t-test, p = 0.19.
    Figure Legend Snippet: TRPM4 mRNA expression is unchanged in cortical neurons from healthy and EAE mice. Cortical nuclei from healthy and EAE-induced mice were sorted by NeuN immunoreactivity. TRPM4 expression levels were assessed by quantitative PCR. n = 5; unpaired 2-tailed t-test, p = 0.19.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    trpm4  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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    Structured Review

    Alomone Labs trpm4
    <t>TRPM4</t> does not contribute to synaptic responses. ( a – d ) Effect of 9-phenanthrol (9-P, 30 µM) on EPSPs recorded from CA1 pyramidal neurons in acute slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( a , c ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in 9-P (black). ( b , d ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in 9-P (n = 5, individual responses, mean ± SEM) ( e – h ) Effect of glibenclamide (Glib. 20 µM) on EPSPs recorded from CA1 pyramidal neurons in slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( e , g ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in glibenclamide (black). ( f , h ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in glibenclamide (n = 6, individual responses, mean ± SEM). Non-normalized data and Cumming’s plots are shown in Supplemental Fig. .
    Trpm4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpm4/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpm4 - by Bioz Stars, 2023-06
    93/100 stars

    Images

    1) Product Images from "A glibenclamide-sensitive TRPM4-mediated component of CA1 excitatory postsynaptic potentials appears in experimental autoimmune encephalomyelitis"

    Article Title: A glibenclamide-sensitive TRPM4-mediated component of CA1 excitatory postsynaptic potentials appears in experimental autoimmune encephalomyelitis

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-09875-6

    TRPM4 does not contribute to synaptic responses. ( a – d ) Effect of 9-phenanthrol (9-P, 30 µM) on EPSPs recorded from CA1 pyramidal neurons in acute slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( a , c ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in 9-P (black). ( b , d ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in 9-P (n = 5, individual responses, mean ± SEM) ( e – h ) Effect of glibenclamide (Glib. 20 µM) on EPSPs recorded from CA1 pyramidal neurons in slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( e , g ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in glibenclamide (black). ( f , h ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in glibenclamide (n = 6, individual responses, mean ± SEM). Non-normalized data and Cumming’s plots are shown in Supplemental Fig. .
    Figure Legend Snippet: TRPM4 does not contribute to synaptic responses. ( a – d ) Effect of 9-phenanthrol (9-P, 30 µM) on EPSPs recorded from CA1 pyramidal neurons in acute slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( a , c ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in 9-P (black). ( b , d ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in 9-P (n = 5, individual responses, mean ± SEM) ( e – h ) Effect of glibenclamide (Glib. 20 µM) on EPSPs recorded from CA1 pyramidal neurons in slices from wildtype ( Trpm4 + / + ) or knockout ( Trpm4 –/– ) littermates. ( e , g ) Sample EPSPs before (blue/orange traces) or 20 min. after washing in glibenclamide (black). ( f , h ) Normalized peak and area under the curve (AUC) averaged from 5 min before (baseline) and the last 5 min in glibenclamide (n = 6, individual responses, mean ± SEM). Non-normalized data and Cumming’s plots are shown in Supplemental Fig. .

    Techniques Used: Knock-Out

    The induction and acute phase of EAE was not different in Trpm4 –/– and Trpm4 + / + littermates. ( a ) Daily clinical scores and ( b ) body weight of Trpm4 –/– (orange circles, n = 6 mice) and Trpm4 + / + (blue circles, n = 6 mice) littermates recorded daily after induction of experimental autoimmune encephalomyelitis (EAE) on day 0 (mean ± SEM). ( c ) EAE clinical progression of individual mice and score at time of sacrifice for electrophysiology (X’s). ( d ) Cell resistance was calculated from 200 ms–10 pA current injections in drug-free ACSF. Cell resistance is negatively correlated with the final clinical score in Trpm4 + / + mice (Pearson’s r = − 0.77, p = 0.0035) but not in Trpm4 –/– mice (Pearson’s r = − 0.2, p = 0.58).
    Figure Legend Snippet: The induction and acute phase of EAE was not different in Trpm4 –/– and Trpm4 + / + littermates. ( a ) Daily clinical scores and ( b ) body weight of Trpm4 –/– (orange circles, n = 6 mice) and Trpm4 + / + (blue circles, n = 6 mice) littermates recorded daily after induction of experimental autoimmune encephalomyelitis (EAE) on day 0 (mean ± SEM). ( c ) EAE clinical progression of individual mice and score at time of sacrifice for electrophysiology (X’s). ( d ) Cell resistance was calculated from 200 ms–10 pA current injections in drug-free ACSF. Cell resistance is negatively correlated with the final clinical score in Trpm4 + / + mice (Pearson’s r = − 0.77, p = 0.0035) but not in Trpm4 –/– mice (Pearson’s r = − 0.2, p = 0.58).

    Techniques Used:

    Only cell resistance significantly varies between genotype or health status at baseline.
    Figure Legend Snippet: Only cell resistance significantly varies between genotype or health status at baseline.

    Techniques Used:

    Glibenclamide, not 9-phenanthrol, reduces EPSP peak amplitude in CA1 pyramidal neurons in EAE mice. ( a ) EPSPs recorded from a representative CA1 pyramidal neuron in response to stimulation of stratum radiatum in an acute slice from a Trpm4 + / + mouse prepared during the acute response to EAE. After establishing a stable baseline (blue trace), 9-phenanthrol (30 μM, 9-P) was washed into the bath for 20 min (black trace). ( b ) Normalized average EPSP peak and area under the curve (AUC) at baseline (average 5 min) and after wash-in of 9-phenanthrol (average 15–20 min) (n = 4, individual experiments, mean ± SEM). ( c ) As in a but black trace is after wash-in of glibenclamide (20 µM, Glib). ( d ) As in B but before and after washing in glibenclamide (n = 8, individual experiments, mean ± SEM, *p = 0.02 Wilcoxon paired test). ( e ) As in C but example EPSPs were recorded before (orange trace) and after washing in glibenclamide (black trace) onto a CA1 neuron in a slice made from a Trpm4 -/- EAE littermate. ( f ) As in D but from Trpm4 -/- littermates (n = 7, individual experiments, mean ± SEM). Non-normalized data and Cumming’s plots are in Supplemental Fig. .
    Figure Legend Snippet: Glibenclamide, not 9-phenanthrol, reduces EPSP peak amplitude in CA1 pyramidal neurons in EAE mice. ( a ) EPSPs recorded from a representative CA1 pyramidal neuron in response to stimulation of stratum radiatum in an acute slice from a Trpm4 + / + mouse prepared during the acute response to EAE. After establishing a stable baseline (blue trace), 9-phenanthrol (30 μM, 9-P) was washed into the bath for 20 min (black trace). ( b ) Normalized average EPSP peak and area under the curve (AUC) at baseline (average 5 min) and after wash-in of 9-phenanthrol (average 15–20 min) (n = 4, individual experiments, mean ± SEM). ( c ) As in a but black trace is after wash-in of glibenclamide (20 µM, Glib). ( d ) As in B but before and after washing in glibenclamide (n = 8, individual experiments, mean ± SEM, *p = 0.02 Wilcoxon paired test). ( e ) As in C but example EPSPs were recorded before (orange trace) and after washing in glibenclamide (black trace) onto a CA1 neuron in a slice made from a Trpm4 -/- EAE littermate. ( f ) As in D but from Trpm4 -/- littermates (n = 7, individual experiments, mean ± SEM). Non-normalized data and Cumming’s plots are in Supplemental Fig. .

    Techniques Used:

    TRPM4 mRNA expression is unchanged in cortical neurons from healthy and EAE mice. Cortical nuclei from healthy and EAE-induced mice were sorted by NeuN immunoreactivity. TRPM4 expression levels were assessed by quantitative PCR. n = 5; unpaired 2-tailed t-test, p = 0.19.
    Figure Legend Snippet: TRPM4 mRNA expression is unchanged in cortical neurons from healthy and EAE mice. Cortical nuclei from healthy and EAE-induced mice were sorted by NeuN immunoreactivity. TRPM4 expression levels were assessed by quantitative PCR. n = 5; unpaired 2-tailed t-test, p = 0.19.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    trpm4  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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    • 93
      Buy from Supplier

    Structured Review

    Alomone Labs trpm4
    Trpm4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpm4/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpm4 - by Bioz Stars, 2023-06
    93/100 stars

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