anti ephrin a1  (Alomone Labs)


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    Structured Review

    Alomone Labs anti ephrin a1
    <t>Ephrin-A1</t> mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P
    Anti Ephrin A1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ephrin a1/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ephrin a1 - by Bioz Stars, 2022-05
    88/100 stars

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    1) Product Images from "Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2"

    Article Title: Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2

    Journal: Nature Communications

    doi: 10.1038/ncomms15728

    Ephrin-A1 mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P
    Figure Legend Snippet: Ephrin-A1 mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P

    Techniques Used: Concentration Assay, Over Expression, Plasmid Preparation

    sEV-associated EphA2 promotes cancer cell proliferation through EphA2/ephrin-A1 reverse signalling. ( a ) Immunoblotting of EphA2 and β-actin in the WCL of MCF-7 cells expressing empty vector or ectopic EphA2. Cells were grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells. ( b ) EphA2 immunoprecipitates prepared from MCF-7 cells expressing ectopic EphA2 were immunoblotted with anti-phosphotyrosine and anti-EphA2 antibody. The indicated samples were treated with recombinant human ephrin-A1 (500 ng ml −1 ) for 20 min. Eight-hour pre-treatment with the EphA2 inhibitor ALW-II-41-27 (100 nM) suppressed ephrin-A1-induced EphA2 phosphorylation. ( c ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or ALW-II-41-27 (100 nM) was added to the medium. ( d ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM prepared from control or DXR-induced senescent RPE-1 cells expressing non-targeting shRNA (shNT), shEphA2 or shRab35. ( e ) The heatmap shows the relative gene expression levels in MCF-7 cells grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells expressing shNT or shEphA2. Genes whose expression levels were changed more than 1.2-fold by the CM of shNT-expressing cells are shown. ( f ) EphA2-dependent upregulated genes were analysed with oPOSSUM-3. Each circle in the plot shows an enrichment of the targets of a different transcription factor. ( g ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM. Dot plot represents the number of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or U0126 (500 nM) was added to the medium. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3). Error bars indicate s.d. * P
    Figure Legend Snippet: sEV-associated EphA2 promotes cancer cell proliferation through EphA2/ephrin-A1 reverse signalling. ( a ) Immunoblotting of EphA2 and β-actin in the WCL of MCF-7 cells expressing empty vector or ectopic EphA2. Cells were grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells. ( b ) EphA2 immunoprecipitates prepared from MCF-7 cells expressing ectopic EphA2 were immunoblotted with anti-phosphotyrosine and anti-EphA2 antibody. The indicated samples were treated with recombinant human ephrin-A1 (500 ng ml −1 ) for 20 min. Eight-hour pre-treatment with the EphA2 inhibitor ALW-II-41-27 (100 nM) suppressed ephrin-A1-induced EphA2 phosphorylation. ( c ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or ALW-II-41-27 (100 nM) was added to the medium. ( d ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM prepared from control or DXR-induced senescent RPE-1 cells expressing non-targeting shRNA (shNT), shEphA2 or shRab35. ( e ) The heatmap shows the relative gene expression levels in MCF-7 cells grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells expressing shNT or shEphA2. Genes whose expression levels were changed more than 1.2-fold by the CM of shNT-expressing cells are shown. ( f ) EphA2-dependent upregulated genes were analysed with oPOSSUM-3. Each circle in the plot shows an enrichment of the targets of a different transcription factor. ( g ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM. Dot plot represents the number of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or U0126 (500 nM) was added to the medium. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3). Error bars indicate s.d. * P

    Techniques Used: Expressing, Plasmid Preparation, Recombinant, shRNA

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    Alomone Labs anti ephrin a1
    <t>Ephrin-A1</t> mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P
    Anti Ephrin A1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ephrin a1/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ephrin a1 - by Bioz Stars, 2022-05
    88/100 stars
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    Ephrin-A1 mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P

    Journal: Nature Communications

    Article Title: Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2

    doi: 10.1038/ncomms15728

    Figure Lengend Snippet: Ephrin-A1 mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P

    Article Snippet: After cooling, the proteins were separated by SDS-PAGE, transferred onto PVDF membranes, blocked with 5% milk or 2% BSA for 1 h and probed with following primary antibodies: anti-Alix (12422-1-AP; Proteintech), anti-β-actin (clone AC-74; SIGMA), anti-CD9 (ab92726; Abcam), anti-ephrin-A1 (AER-031; Alomone Labs), anti-EphA2 (C-20; Santa Cruz), anti-Erk (#9102; CST), anti-FLAG (PM020; MBL), anti-p21 (C-19; Santa Cruz), anti-phospho-Erk (E-4; Santa Cruz), anti-phospho-p53 (Ser15) (#9284; CST), anti-phosphotyrosine (clone 4G10; Millipore), anti-PTP1B (clone FG6-1G; Millipore) and anti-Rab35 (11329-2-AP; Proteintech).

    Techniques: Concentration Assay, Over Expression, Plasmid Preparation

    sEV-associated EphA2 promotes cancer cell proliferation through EphA2/ephrin-A1 reverse signalling. ( a ) Immunoblotting of EphA2 and β-actin in the WCL of MCF-7 cells expressing empty vector or ectopic EphA2. Cells were grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells. ( b ) EphA2 immunoprecipitates prepared from MCF-7 cells expressing ectopic EphA2 were immunoblotted with anti-phosphotyrosine and anti-EphA2 antibody. The indicated samples were treated with recombinant human ephrin-A1 (500 ng ml −1 ) for 20 min. Eight-hour pre-treatment with the EphA2 inhibitor ALW-II-41-27 (100 nM) suppressed ephrin-A1-induced EphA2 phosphorylation. ( c ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or ALW-II-41-27 (100 nM) was added to the medium. ( d ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM prepared from control or DXR-induced senescent RPE-1 cells expressing non-targeting shRNA (shNT), shEphA2 or shRab35. ( e ) The heatmap shows the relative gene expression levels in MCF-7 cells grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells expressing shNT or shEphA2. Genes whose expression levels were changed more than 1.2-fold by the CM of shNT-expressing cells are shown. ( f ) EphA2-dependent upregulated genes were analysed with oPOSSUM-3. Each circle in the plot shows an enrichment of the targets of a different transcription factor. ( g ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM. Dot plot represents the number of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or U0126 (500 nM) was added to the medium. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3). Error bars indicate s.d. * P

    Journal: Nature Communications

    Article Title: Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2

    doi: 10.1038/ncomms15728

    Figure Lengend Snippet: sEV-associated EphA2 promotes cancer cell proliferation through EphA2/ephrin-A1 reverse signalling. ( a ) Immunoblotting of EphA2 and β-actin in the WCL of MCF-7 cells expressing empty vector or ectopic EphA2. Cells were grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells. ( b ) EphA2 immunoprecipitates prepared from MCF-7 cells expressing ectopic EphA2 were immunoblotted with anti-phosphotyrosine and anti-EphA2 antibody. The indicated samples were treated with recombinant human ephrin-A1 (500 ng ml −1 ) for 20 min. Eight-hour pre-treatment with the EphA2 inhibitor ALW-II-41-27 (100 nM) suppressed ephrin-A1-induced EphA2 phosphorylation. ( c ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or ALW-II-41-27 (100 nM) was added to the medium. ( d ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM prepared from control or DXR-induced senescent RPE-1 cells expressing non-targeting shRNA (shNT), shEphA2 or shRab35. ( e ) The heatmap shows the relative gene expression levels in MCF-7 cells grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells expressing shNT or shEphA2. Genes whose expression levels were changed more than 1.2-fold by the CM of shNT-expressing cells are shown. ( f ) EphA2-dependent upregulated genes were analysed with oPOSSUM-3. Each circle in the plot shows an enrichment of the targets of a different transcription factor. ( g ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM. Dot plot represents the number of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or U0126 (500 nM) was added to the medium. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3). Error bars indicate s.d. * P

    Article Snippet: After cooling, the proteins were separated by SDS-PAGE, transferred onto PVDF membranes, blocked with 5% milk or 2% BSA for 1 h and probed with following primary antibodies: anti-Alix (12422-1-AP; Proteintech), anti-β-actin (clone AC-74; SIGMA), anti-CD9 (ab92726; Abcam), anti-ephrin-A1 (AER-031; Alomone Labs), anti-EphA2 (C-20; Santa Cruz), anti-Erk (#9102; CST), anti-FLAG (PM020; MBL), anti-p21 (C-19; Santa Cruz), anti-phospho-Erk (E-4; Santa Cruz), anti-phospho-p53 (Ser15) (#9284; CST), anti-phosphotyrosine (clone 4G10; Millipore), anti-PTP1B (clone FG6-1G; Millipore) and anti-Rab35 (11329-2-AP; Proteintech).

    Techniques: Expressing, Plasmid Preparation, Recombinant, shRNA