anti ephrin a1  (Alomone Labs)


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    Name:
    Anti Ephrin A1 extracellular Antibody
    Description:
    Anti Ephrin A1 extracellular Antibody is directed against an extracellular epitope of human Ephrin A1 Anti Ephrin A1 extracellular Antibody AER 031 can be used in western blot and live cell imaging applications The antibody recognizes an extracellular epitope and thus is ideal for detecting Ephrin A1 in living cells It has been designed to recognize Ephrin A1 from human rat and mouse samples
    Catalog Number:
    AER-031
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs anti ephrin a1
    Anti Ephrin A1 extracellular Antibody
    Anti Ephrin A1 extracellular Antibody is directed against an extracellular epitope of human Ephrin A1 Anti Ephrin A1 extracellular Antibody AER 031 can be used in western blot and live cell imaging applications The antibody recognizes an extracellular epitope and thus is ideal for detecting Ephrin A1 in living cells It has been designed to recognize Ephrin A1 from human rat and mouse samples
    https://www.bioz.com/result/anti ephrin a1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ephrin a1 - by Bioz Stars, 2021-09
    90/100 stars

    Images

    1) Product Images from "Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2"

    Article Title: Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2

    Journal: Nature Communications

    doi: 10.1038/ncomms15728

    Ephrin-A1 mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P
    Figure Legend Snippet: Ephrin-A1 mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P

    Techniques Used: Concentration Assay, Over Expression, Plasmid Preparation

    sEV-associated EphA2 promotes cancer cell proliferation through EphA2/ephrin-A1 reverse signalling. ( a ) Immunoblotting of EphA2 and β-actin in the WCL of MCF-7 cells expressing empty vector or ectopic EphA2. Cells were grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells. ( b ) EphA2 immunoprecipitates prepared from MCF-7 cells expressing ectopic EphA2 were immunoblotted with anti-phosphotyrosine and anti-EphA2 antibody. The indicated samples were treated with recombinant human ephrin-A1 (500 ng ml −1 ) for 20 min. Eight-hour pre-treatment with the EphA2 inhibitor ALW-II-41-27 (100 nM) suppressed ephrin-A1-induced EphA2 phosphorylation. ( c ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or ALW-II-41-27 (100 nM) was added to the medium. ( d ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM prepared from control or DXR-induced senescent RPE-1 cells expressing non-targeting shRNA (shNT), shEphA2 or shRab35. ( e ) The heatmap shows the relative gene expression levels in MCF-7 cells grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells expressing shNT or shEphA2. Genes whose expression levels were changed more than 1.2-fold by the CM of shNT-expressing cells are shown. ( f ) EphA2-dependent upregulated genes were analysed with oPOSSUM-3. Each circle in the plot shows an enrichment of the targets of a different transcription factor. ( g ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM. Dot plot represents the number of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or U0126 (500 nM) was added to the medium. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3). Error bars indicate s.d. * P
    Figure Legend Snippet: sEV-associated EphA2 promotes cancer cell proliferation through EphA2/ephrin-A1 reverse signalling. ( a ) Immunoblotting of EphA2 and β-actin in the WCL of MCF-7 cells expressing empty vector or ectopic EphA2. Cells were grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells. ( b ) EphA2 immunoprecipitates prepared from MCF-7 cells expressing ectopic EphA2 were immunoblotted with anti-phosphotyrosine and anti-EphA2 antibody. The indicated samples were treated with recombinant human ephrin-A1 (500 ng ml −1 ) for 20 min. Eight-hour pre-treatment with the EphA2 inhibitor ALW-II-41-27 (100 nM) suppressed ephrin-A1-induced EphA2 phosphorylation. ( c ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or ALW-II-41-27 (100 nM) was added to the medium. ( d ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM prepared from control or DXR-induced senescent RPE-1 cells expressing non-targeting shRNA (shNT), shEphA2 or shRab35. ( e ) The heatmap shows the relative gene expression levels in MCF-7 cells grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells expressing shNT or shEphA2. Genes whose expression levels were changed more than 1.2-fold by the CM of shNT-expressing cells are shown. ( f ) EphA2-dependent upregulated genes were analysed with oPOSSUM-3. Each circle in the plot shows an enrichment of the targets of a different transcription factor. ( g ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM. Dot plot represents the number of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or U0126 (500 nM) was added to the medium. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3). Error bars indicate s.d. * P

    Techniques Used: Expressing, Plasmid Preparation, Recombinant, shRNA

    Related Articles

    SDS Page:

    Article Title: Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2
    Article Snippet: .. After cooling, the proteins were separated by SDS-PAGE, transferred onto PVDF membranes, blocked with 5% milk or 2% BSA for 1 h and probed with following primary antibodies: anti-Alix (12422-1-AP; Proteintech), anti-β-actin (clone AC-74; SIGMA), anti-CD9 (ab92726; Abcam), anti-ephrin-A1 (AER-031; Alomone Labs), anti-EphA2 (C-20; Santa Cruz), anti-Erk (#9102; CST), anti-FLAG (PM020; MBL), anti-p21 (C-19; Santa Cruz), anti-phospho-Erk (E-4; Santa Cruz), anti-phospho-p53 (Ser15) (#9284; CST), anti-phosphotyrosine (clone 4G10; Millipore), anti-PTP1B (clone FG6-1G; Millipore) and anti-Rab35 (11329-2-AP; Proteintech). ..

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  • 90
    Alomone Labs anti ephrin a1
    <t>Ephrin-A1</t> mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P
    Anti Ephrin A1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ephrin a1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ephrin a1 - by Bioz Stars, 2021-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Ephrin-A1 mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P

    Journal: Nature Communications

    Article Title: Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2

    doi: 10.1038/ncomms15728

    Figure Lengend Snippet: Ephrin-A1 mediates the growth-promoting effect of sEV-associated EphA2. ( a ) Immunoblotting of EphA2, ephrin-A1 and β-actin in the WCL of indicated cell lines. ( b ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Normal rabbit IgG or rabbit anti-ephrin-A1 IgG (AER-031; Alomone) was added to the medium at a concentration of 5 μg ml −1 . CM was prepared from DXR-induced senescent RPE-1 cells. ( c ) Immunoblotting shows successful overexpression of ephrin-A1 in MCF-7 cells. Dot plot represents the relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. Empty vector or ectopic ephrin-A1 was expressed in MCF-7 cells. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3 for b and n =5 for c ). Error bars indicate s.d. * P

    Article Snippet: After cooling, the proteins were separated by SDS-PAGE, transferred onto PVDF membranes, blocked with 5% milk or 2% BSA for 1 h and probed with following primary antibodies: anti-Alix (12422-1-AP; Proteintech), anti-β-actin (clone AC-74; SIGMA), anti-CD9 (ab92726; Abcam), anti-ephrin-A1 (AER-031; Alomone Labs), anti-EphA2 (C-20; Santa Cruz), anti-Erk (#9102; CST), anti-FLAG (PM020; MBL), anti-p21 (C-19; Santa Cruz), anti-phospho-Erk (E-4; Santa Cruz), anti-phospho-p53 (Ser15) (#9284; CST), anti-phosphotyrosine (clone 4G10; Millipore), anti-PTP1B (clone FG6-1G; Millipore) and anti-Rab35 (11329-2-AP; Proteintech).

    Techniques: Concentration Assay, Over Expression, Plasmid Preparation

    sEV-associated EphA2 promotes cancer cell proliferation through EphA2/ephrin-A1 reverse signalling. ( a ) Immunoblotting of EphA2 and β-actin in the WCL of MCF-7 cells expressing empty vector or ectopic EphA2. Cells were grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells. ( b ) EphA2 immunoprecipitates prepared from MCF-7 cells expressing ectopic EphA2 were immunoblotted with anti-phosphotyrosine and anti-EphA2 antibody. The indicated samples were treated with recombinant human ephrin-A1 (500 ng ml −1 ) for 20 min. Eight-hour pre-treatment with the EphA2 inhibitor ALW-II-41-27 (100 nM) suppressed ephrin-A1-induced EphA2 phosphorylation. ( c ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or ALW-II-41-27 (100 nM) was added to the medium. ( d ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM prepared from control or DXR-induced senescent RPE-1 cells expressing non-targeting shRNA (shNT), shEphA2 or shRab35. ( e ) The heatmap shows the relative gene expression levels in MCF-7 cells grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells expressing shNT or shEphA2. Genes whose expression levels were changed more than 1.2-fold by the CM of shNT-expressing cells are shown. ( f ) EphA2-dependent upregulated genes were analysed with oPOSSUM-3. Each circle in the plot shows an enrichment of the targets of a different transcription factor. ( g ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM. Dot plot represents the number of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or U0126 (500 nM) was added to the medium. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3). Error bars indicate s.d. * P

    Journal: Nature Communications

    Article Title: Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2

    doi: 10.1038/ncomms15728

    Figure Lengend Snippet: sEV-associated EphA2 promotes cancer cell proliferation through EphA2/ephrin-A1 reverse signalling. ( a ) Immunoblotting of EphA2 and β-actin in the WCL of MCF-7 cells expressing empty vector or ectopic EphA2. Cells were grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells. ( b ) EphA2 immunoprecipitates prepared from MCF-7 cells expressing ectopic EphA2 were immunoblotted with anti-phosphotyrosine and anti-EphA2 antibody. The indicated samples were treated with recombinant human ephrin-A1 (500 ng ml −1 ) for 20 min. Eight-hour pre-treatment with the EphA2 inhibitor ALW-II-41-27 (100 nM) suppressed ephrin-A1-induced EphA2 phosphorylation. ( c ) Relative numbers of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or ALW-II-41-27 (100 nM) was added to the medium. ( d ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM prepared from control or DXR-induced senescent RPE-1 cells expressing non-targeting shRNA (shNT), shEphA2 or shRab35. ( e ) The heatmap shows the relative gene expression levels in MCF-7 cells grown for 3 days with or without CM prepared from DXR-induced senescent RPE-1 cells expressing shNT or shEphA2. Genes whose expression levels were changed more than 1.2-fold by the CM of shNT-expressing cells are shown. ( f ) EphA2-dependent upregulated genes were analysed with oPOSSUM-3. Each circle in the plot shows an enrichment of the targets of a different transcription factor. ( g ) Immunoblotting of phospho-Erk, total-Erk and β-actin in the WCL of MCF-7 cells grown for 3 days with or without CM. Dot plot represents the number of MCF-7 cells grown for 3 days in the presence of CM compared with the number of cells grown for 3 days in normal medium. DMSO or U0126 (500 nM) was added to the medium. CM was prepared from DXR-induced senescent RPE-1 cells. Replicates are biological replicates ( n =3). Error bars indicate s.d. * P

    Article Snippet: After cooling, the proteins were separated by SDS-PAGE, transferred onto PVDF membranes, blocked with 5% milk or 2% BSA for 1 h and probed with following primary antibodies: anti-Alix (12422-1-AP; Proteintech), anti-β-actin (clone AC-74; SIGMA), anti-CD9 (ab92726; Abcam), anti-ephrin-A1 (AER-031; Alomone Labs), anti-EphA2 (C-20; Santa Cruz), anti-Erk (#9102; CST), anti-FLAG (PM020; MBL), anti-p21 (C-19; Santa Cruz), anti-phospho-Erk (E-4; Santa Cruz), anti-phospho-p53 (Ser15) (#9284; CST), anti-phosphotyrosine (clone 4G10; Millipore), anti-PTP1B (clone FG6-1G; Millipore) and anti-Rab35 (11329-2-AP; Proteintech).

    Techniques: Expressing, Plasmid Preparation, Recombinant, shRNA