Ip3r1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene"
Article Title: Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene
Journal: Nucleic Acids Research
Figure Legend Snippet: GFP expression in the brains of adult Tg( eno2:GFP ) zebrafish. ( A ) Parasagittal sections of Tg( eno2:GFP ) Pt404 (upper panel) and wild-type (lower panel) brain were labelled with an antibody to GFP. Bound antibody was detected using a histochemical reaction with a red product and nuclei were labelled with a blue counterstain. GFP expression was apparent throughout the brain of transgenic adults. ( B ) High-power view of medullary reticular formation of Tg( eno2:GFP ) Pt404 zebrafish brain, showing GFP expression in cells with neuronal morphology. The arrows show GFP-expressing neurons with axons in the plane of the section. ( C – F ) Double label confocal images of Tg( eno2:GFP ) Pt404 adult brains. Each panel consists of a set of three images: (i) Upper image; red: a cell type-specific marker of interest was localized using specific antibodies. C: Medulla; ChAT, choline acetyltransferase; cholinergic neurons; D: Olfactory bulb; TH, tyrosine hydroxylase; dopaminergic neurons; E: cerebellum; IP3R1, IP3 receptor 1; cerebellar Purkinje cells; D: thalamus; GABA, γ-amino butyric acid; inhibitory neurons; (ii) Middle image, green: GFP expression was localized in the same sections using a GFP antibody; (iii) Lower image: the merged images show co-localization of GFP and the cell type-specific marker (yellow), and were counterstained with DAPI to show nuclei (blue). The scale bar (10 µm) for each set of images is shown in the lower panel.
Techniques Used: Expressing, Transgenic Assay, Marker