tmem16a (Alomone Labs)


Structured Review

Tmem16a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tmem16a/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Pathogenic Roles of Ion Channels and Transporters: Dextran sulfate sodium-induced chronic colitis attenuates Ca2+-activated Cl− secretion in murine colon by downregulating TMEM16A"
Article Title: Pathogenic Roles of Ion Channels and Transporters: Dextran sulfate sodium-induced chronic colitis attenuates Ca2+-activated Cl− secretion in murine colon by downregulating TMEM16A
Journal: American Journal of Physiology - Cell Physiology
doi: 10.1152/ajpcell.00328.2017

Figure Legend Snippet: Effect of dextran sulfate sodium (DSS)-colitis on Cl − channel protein expression in murine colon. A : representative images of protein expression of the different characterized Cl − channels from epithelial lysates. BEST2, bestrophin-2. B : transmembrane protein 16A (TMEM16A) protein expression normalized to β-actin for quantification of control and DSS-colitis groups. TMEM16A expression (closed bar) is significantly decreased in DSS-colitis mice as compared with control. C and D : summarized data of Western blot quantification of BEST2 and CFTR normalized to β-actin in control and DSS-colitis mice. Neither protein was significantly altered from the control cohort. Bar graphs of summarized data represent means ± SE of 5 different animals from each respective group. * P
Techniques Used: Expressing, Mouse Assay, Western Blot

Figure Legend Snippet: Immunohistochemistry of transmembrane protein 16A (TMEM16A) in colonic tissue sections. A : immunohistochemistry of TMEM16A in control tissue demonstrates pronounced expression and localization of the protein to surface epithelium and upper crypts ( top , left ). B. DSS-colitis tissue stained for TMEM16A exhibits less staining in epithelial cells and a more diffuse signal in the lamina propria ( bottom , left ). C and D : both cohorts of tissue were run in parallel with a control peptide specific for the anti-TMEM16A antibody. Both images demonstrate a lack of staining when coincubated with control peptide. Images were captured at ×20 magnification on the AxioImager (Carl Zeiss, Germany).
Techniques Used: Immunohistochemistry, Expressing, Staining

Figure Legend Snippet: Effect of dextran sulfate sodium (DSS)-colitis on transcript abundance of Cl − channels in murine colon. Transcript abundance was determined for transmembrane protein 16A ( Tmem16a ; A ), bestrophin-2 ( Best2 ; B ), and Cftr ( C ) in control (open bars) or DSS-colitis mice (closed bars). Bar graphs of summarized data represent means ± SE of 5 different animals normalized to an endogenous control, Actb . * P
Techniques Used: Mouse Assay