Synaptotagmin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "WT1-Expressing Interneurons Regulate Left–Right Alternation during Mammalian Locomotor Activity"
Article Title: WT1-Expressing Interneurons Regulate Left–Right Alternation during Mammalian Locomotor Activity
Journal: The Journal of Neuroscience
Figure Legend Snippet: WT1 -expressing neurons terminate in close proximity to populations of commissurally projecting interneurons. A , A 20-μm-thick section of a spinal cord 46 h after PRV-152 injection into the GS muscle on the left side and stained with antibodies to GFP (green) and WT1 (blue). At this time point, no WT1 -expressing neurons on either side of the spinal cord have taken up the tracer. Contralateral region containing WT1 -expressing cells (dashed box) is expanded to the right. B , To identify presumptive synapses on genetically defined interneuronal subtypes, we inspected the synaptotagmin + “halo” (indicated by white arrows) surrounding each labeled nuclei for WT1 + /synaptotagmin + terminals. C–F , The 20-μm-thick sections cut from a P0 WT1 CreER ROSA26 tdTomato spinal cord and stained with antibodies to tdTomato (red), the synaptic marker synatotagmin (blue), as well as (green) a nuclear marker of V2a cells ( Chx10 -, C ), V1 cells ( En1 -, D ), V0 V cells ( Evx1 -, E ), or DMRT3 -expressing dI6 cells ( F ). WT1 -expressing terminals (tdTomato + /synaptotagmin + processes) were rare or absent nearby Chx10 - or En1 -expressing cells but were commonly seen in close proximity to Evx1 + and DMRT3 + neurons. C , D , Dashed boxes are expanded to the right. E , F , Arrow in the low-magnification image indicates the specific WT1 cell of interest in the panel to the right. In magnified images, arrows indicate presumptive WT1 + axon terminals. Double labeling of these processes is confirmed in orthogonal views to the right of E , F . Scale bars: Low-magnification images, 100 μm; High-magnification images: A , C , D , 20 μm; B , E , F , 5 μm.
Techniques Used: Expressing, Injection, Staining, Labeling, Marker