anti endothelin receptor a antibody  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Alomone Labs anti endothelin receptor a antibody
    Small pulmonary artery profiles show increasing vasoconstriction when treated with a cumulative dose response of ET-1. Shown is a representative profile from a BALB-STD mouse precision cut lung slices sample. ET-1, <t>endothelin-1;</t> STD, short-term depletion.
    Anti Endothelin Receptor A Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti endothelin receptor a antibody/product/Alomone Labs
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti endothelin receptor a antibody - by Bioz Stars, 2022-11
    92/100 stars

    Images

    1) Product Images from "Vascular Dysfunction in Pneumocystis-Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration"

    Article Title: Vascular Dysfunction in Pneumocystis-Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2015.10.008

    Small pulmonary artery profiles show increasing vasoconstriction when treated with a cumulative dose response of ET-1. Shown is a representative profile from a BALB-STD mouse precision cut lung slices sample. ET-1, endothelin-1; STD, short-term depletion.
    Figure Legend Snippet: Small pulmonary artery profiles show increasing vasoconstriction when treated with a cumulative dose response of ET-1. Shown is a representative profile from a BALB-STD mouse precision cut lung slices sample. ET-1, endothelin-1; STD, short-term depletion.

    Techniques Used:

    The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10 −10 to 10 −9 ET-1. B: This coincides with a significant increase in the ET A R in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 ( B ) or n = 16 to 35 profiles per group across 4 to 5 mice ( A ) in two independent experiments. ∗ P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ET A R, endothelin receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.
    Figure Legend Snippet: The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10 −10 to 10 −9 ET-1. B: This coincides with a significant increase in the ET A R in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 ( B ) or n = 16 to 35 profiles per group across 4 to 5 mice ( A ) in two independent experiments. ∗ P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ET A R, endothelin receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

    Techniques Used: Mouse Assay, Western Blot

    ET-1 concentrations are not significantly different in BALB-STD or IFN-γ-STD mice compared with controls. Samples are peptide extracts from total lung homogenates. Data are expressed as means ± SEM. n = 5 mice in two independent experiments. CON, control; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.
    Figure Legend Snippet: ET-1 concentrations are not significantly different in BALB-STD or IFN-γ-STD mice compared with controls. Samples are peptide extracts from total lung homogenates. Data are expressed as means ± SEM. n = 5 mice in two independent experiments. CON, control; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

    Techniques Used: Mouse Assay

    2) Product Images from "Vascular Dysfunction in Pneumocystis-Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration"

    Article Title: Vascular Dysfunction in Pneumocystis-Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2015.10.008

    The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10 −10 to 10 −9 ET-1. B: This coincides with a significant increase in the ET A R in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 ( B ) or n = 16 to 35 profiles per group across 4 to 5 mice ( A ) in two independent experiments. ∗ P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ET A R, endothelin receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.
    Figure Legend Snippet: The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10 −10 to 10 −9 ET-1. B: This coincides with a significant increase in the ET A R in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 ( B ) or n = 16 to 35 profiles per group across 4 to 5 mice ( A ) in two independent experiments. ∗ P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ET A R, endothelin receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

    Techniques Used: Mouse Assay, Western Blot

    3) Product Images from "Therapeutic Hypothermia Activates the Endothelin and Nitric Oxide Systems after Cardiac Arrest in a Pig Model of Cardiopulmonary Resuscitation"

    Article Title: Therapeutic Hypothermia Activates the Endothelin and Nitric Oxide Systems after Cardiac Arrest in a Pig Model of Cardiopulmonary Resuscitation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064792

    Effects of ROSC and MTH on ETAR and ETBR mRNA expression analysis. Endothelin A receptor, ETAR (A), and endothelin B receptor, ETBR (B), mRNA expression analysis was performed by real-time qPCR. The relative fold changes to control animals with untreated cardiac arrest (C group) were normalized with β-actin and calculated for animals after 30, 60 and 180 min of untreated return of spontaneous circulation (ROSC30, ROSC60 and ROSC180 groups) or after 180 min of mild therapeutic hypothermia (MTH group). Error bars represent standard error of the mean (SEM). Significant results (p
    Figure Legend Snippet: Effects of ROSC and MTH on ETAR and ETBR mRNA expression analysis. Endothelin A receptor, ETAR (A), and endothelin B receptor, ETBR (B), mRNA expression analysis was performed by real-time qPCR. The relative fold changes to control animals with untreated cardiac arrest (C group) were normalized with β-actin and calculated for animals after 30, 60 and 180 min of untreated return of spontaneous circulation (ROSC30, ROSC60 and ROSC180 groups) or after 180 min of mild therapeutic hypothermia (MTH group). Error bars represent standard error of the mean (SEM). Significant results (p

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    Western Blot analysis of ETAR and ETBR. Cardiac left ventricle tissue homogenates from control animals with untreated cardiac arrest (C group), or after 180 min of untreated return of spontaneous circulation (ROSC180 group), after 180 min of mild therapeutic hypothermia (MTH group) or 180 min after S-PBN infusion (S-PBN group) were loaded on a 12% SDS-acrylamide gel. Bands for endothelin A receptor (ETAR), endothelin B receptor (ETBR) and β-actin were detected at 25 kDa, 50 kDa and 42 kDa, respectively (A). Protein band intensities were quantified using a ChemiDoc XRS and Quantity One software and normalized to the loading control (β-actin) (B). Error bars represent standard error of the mean (SEM). Significant results (p
    Figure Legend Snippet: Western Blot analysis of ETAR and ETBR. Cardiac left ventricle tissue homogenates from control animals with untreated cardiac arrest (C group), or after 180 min of untreated return of spontaneous circulation (ROSC180 group), after 180 min of mild therapeutic hypothermia (MTH group) or 180 min after S-PBN infusion (S-PBN group) were loaded on a 12% SDS-acrylamide gel. Bands for endothelin A receptor (ETAR), endothelin B receptor (ETBR) and β-actin were detected at 25 kDa, 50 kDa and 42 kDa, respectively (A). Protein band intensities were quantified using a ChemiDoc XRS and Quantity One software and normalized to the loading control (β-actin) (B). Error bars represent standard error of the mean (SEM). Significant results (p

    Techniques Used: Western Blot, Acrylamide Gel Assay, Software

    Effects of S-PBN on ET-1, ECE-1, ETAR and ETBR mRNA expression. Endothelin 1, ET-1 (A), endothelin converting enzyme 1, ECE-1 (B), endothelin A receptor, ETAR (C), and endothelin B receptor, ETBR (D), mRNA expression analysis was performed by real-time qPCR. The relative fold changes to control animals with untreated cardiac arrest (C group) were normalized with β-actin and calculated for animals after 180 min of untreated return of spontaneous circulation (ROSC180 group) or 180 min after S-PBN infusion (S-PBN group). Error bars represent standard error of the mean (SEM). Significant results (p
    Figure Legend Snippet: Effects of S-PBN on ET-1, ECE-1, ETAR and ETBR mRNA expression. Endothelin 1, ET-1 (A), endothelin converting enzyme 1, ECE-1 (B), endothelin A receptor, ETAR (C), and endothelin B receptor, ETBR (D), mRNA expression analysis was performed by real-time qPCR. The relative fold changes to control animals with untreated cardiac arrest (C group) were normalized with β-actin and calculated for animals after 180 min of untreated return of spontaneous circulation (ROSC180 group) or 180 min after S-PBN infusion (S-PBN group). Error bars represent standard error of the mean (SEM). Significant results (p

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    4) Product Images from "Endothelin-2-Mediated Protection of Mutant Photoreceptors in Inherited Photoreceptor Degeneration"

    Article Title: Endothelin-2-Mediated Protection of Mutant Photoreceptors in Inherited Photoreceptor Degeneration

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0058023

    Expression of GFP and Edn2 from subretinally injected scAAV5. (A) The temporal and spatial expression of GFP in WT retinas injected subretinally with 1X PBS or scAAV5-smCBA- Gfp at PN8 and evaluated at PN10, PN12, and PN14 by GFP immunofluorescence (A, Panels 1–4). Significant GFP staining was not observed until PN12, with stronger staining at PN14, especially in the vicinity of the subretinal injection site (arrow). The spatial expression of GFP in WT retinas injected with scAAV5-smCBA- Gfp was also evaluated in paraffin sections at PN12 (A, Panel 5). GFP expression was observed predominantly in the ONL and RPE; sporadic expression of GFP in Müller cells was also observed in paraffin sections. (Bar = 25 µm.) (B) Schematic of the EDN2 cleavage events required to produce the mature EDN2 peptide. EDN2 is first produced as prepro EDN2 (175 aa) which is rapidly processed by furin-like endopeptidases to yield big EDN2 (38 aa). Big EDN2 must then be cleaved by an endothelin-specific converting enzyme (ECE) to produce the 21 a.a. mature EDN2 peptide that can bind to endothelin receptors (figure adapted from [68] ). The regions of the Edn2 mRNA corresponding to the cDNAs cloned into the scAAV5- smCBA -prepro Edn2 and scAAV5- smCBA -mat Edn2 vectors are shown. (C) Expression of scAAV5-derived Edn2 mRNA in Pde6b rd1/rd1 retinas at PN12 after injection of the scAAV5- smCBA -prepro Edn2 and scAAV5- smCBA -mat Edn2 constructs at PN8. scAAV5-derived Edn2 mRNA expression values are shown relative to the levels of endogenous Edn2 mRNA (from the same retina) and all values were normalized to Gapdh . scAAV5-prepro Edn2 transcripts were increased between 1.7 and 7.2-fold (n = 4; average 4.3-fold) over endogenous Edn2 mRNA, while scAAV5-mat Edn2 transcripts increased between 2.5 to 11.3-fold over the endogenous Edn2 mRNA (n = 4; average 6.9-fold). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Error bars indicate SEM.
    Figure Legend Snippet: Expression of GFP and Edn2 from subretinally injected scAAV5. (A) The temporal and spatial expression of GFP in WT retinas injected subretinally with 1X PBS or scAAV5-smCBA- Gfp at PN8 and evaluated at PN10, PN12, and PN14 by GFP immunofluorescence (A, Panels 1–4). Significant GFP staining was not observed until PN12, with stronger staining at PN14, especially in the vicinity of the subretinal injection site (arrow). The spatial expression of GFP in WT retinas injected with scAAV5-smCBA- Gfp was also evaluated in paraffin sections at PN12 (A, Panel 5). GFP expression was observed predominantly in the ONL and RPE; sporadic expression of GFP in Müller cells was also observed in paraffin sections. (Bar = 25 µm.) (B) Schematic of the EDN2 cleavage events required to produce the mature EDN2 peptide. EDN2 is first produced as prepro EDN2 (175 aa) which is rapidly processed by furin-like endopeptidases to yield big EDN2 (38 aa). Big EDN2 must then be cleaved by an endothelin-specific converting enzyme (ECE) to produce the 21 a.a. mature EDN2 peptide that can bind to endothelin receptors (figure adapted from [68] ). The regions of the Edn2 mRNA corresponding to the cDNAs cloned into the scAAV5- smCBA -prepro Edn2 and scAAV5- smCBA -mat Edn2 vectors are shown. (C) Expression of scAAV5-derived Edn2 mRNA in Pde6b rd1/rd1 retinas at PN12 after injection of the scAAV5- smCBA -prepro Edn2 and scAAV5- smCBA -mat Edn2 constructs at PN8. scAAV5-derived Edn2 mRNA expression values are shown relative to the levels of endogenous Edn2 mRNA (from the same retina) and all values were normalized to Gapdh . scAAV5-prepro Edn2 transcripts were increased between 1.7 and 7.2-fold (n = 4; average 4.3-fold) over endogenous Edn2 mRNA, while scAAV5-mat Edn2 transcripts increased between 2.5 to 11.3-fold over the endogenous Edn2 mRNA (n = 4; average 6.9-fold). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Error bars indicate SEM.

    Techniques Used: Expressing, Injection, Immunofluorescence, Staining, Produced, Clone Assay, Derivative Assay, Construct

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Alomone Labs anti endothelin receptor a antibody
    Anti Endothelin Receptor A Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti endothelin receptor a antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti endothelin receptor a antibody - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

    Image Search Results