anti d2 dopamine receptor extracellular antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti d2 dopamine receptor extracellular antibody
    Quantitative PCR and western blot analyses of DA and 5-HT transporters, and dopamine D1 and D2 receptors in the striatum. qPCR (A–D) and western blot (E–I) analyses showing that no significant differences in the expression levels of DA transporter (DAT: A,E,I ), 5-HT transporter (5HTT: B,F,I ), D1 receptor (D1R: C,G,I ), and <t>D2</t> receptor (D2R: D,H,I ) were observed between FFAR1+/+ and −/− mice. n = 6 (A) and 5 (B-D) each. In (I) , n = 4 (+/+) and 6 (−/−) for DAT, n = 8.
    Anti D2 Dopamine Receptor Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti d2 dopamine receptor extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    anti d2 dopamine receptor extracellular antibody - by Bioz Stars, 2022-11
    93/100 stars

    Images

    1) Product Images from "FFAR1/GPR40 Contributes to the Regulation of Striatal Monoamine Releases and Facilitation of Cocaine-Induced Locomotor Activity in Mice"

    Article Title: FFAR1/GPR40 Contributes to the Regulation of Striatal Monoamine Releases and Facilitation of Cocaine-Induced Locomotor Activity in Mice

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.699026

    Quantitative PCR and western blot analyses of DA and 5-HT transporters, and dopamine D1 and D2 receptors in the striatum. qPCR (A–D) and western blot (E–I) analyses showing that no significant differences in the expression levels of DA transporter (DAT: A,E,I ), 5-HT transporter (5HTT: B,F,I ), D1 receptor (D1R: C,G,I ), and D2 receptor (D2R: D,H,I ) were observed between FFAR1+/+ and −/− mice. n = 6 (A) and 5 (B-D) each. In (I) , n = 4 (+/+) and 6 (−/−) for DAT, n = 8.
    Figure Legend Snippet: Quantitative PCR and western blot analyses of DA and 5-HT transporters, and dopamine D1 and D2 receptors in the striatum. qPCR (A–D) and western blot (E–I) analyses showing that no significant differences in the expression levels of DA transporter (DAT: A,E,I ), 5-HT transporter (5HTT: B,F,I ), D1 receptor (D1R: C,G,I ), and D2 receptor (D2R: D,H,I ) were observed between FFAR1+/+ and −/− mice. n = 6 (A) and 5 (B-D) each. In (I) , n = 4 (+/+) and 6 (−/−) for DAT, n = 8.

    Techniques Used: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Mouse Assay

    2) Product Images from "Activation of Dopamine D1-D2 Receptor Complex Attenuates Cocaine Reward and Reinstatement of Cocaine-Seeking through Inhibition of DARPP-32, ERK, and ΔFosB"

    Article Title: Activation of Dopamine D1-D2 Receptor Complex Attenuates Cocaine Reward and Reinstatement of Cocaine-Seeking through Inhibition of DARPP-32, ERK, and ΔFosB

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00924

    The effects of D1-D2 heteromer stimulation and inactivation on basal conditioned place preference. (A) Vehicle-conditioned rats did not exhibit a preference toward a particular chamber. D1-D2 heteromer stimulation by SKF 83959 (1.5 mg/kg, s.c.) induced conditioned place aversion (CPA) as the animals spent significantly less time in the drug paired chamber. (B) SKF 83959-induced CPA was abolished by pre-treatment by the D1-D2 heteromer selective disrupting peptide, TAT-D1, but not the control TAT-Sc peptide. (C) Inactivation of D1-D2 heteromer by TAT-D1 resulted in conditioned place preference (CPP) as the rats spent significantly more time in the drug paired chamber, not observed with the control TAT-Sc. (D) Representative western blots (inset) and histogram showing the amount of D1R co-immunoprecipitated with D2R from the NAc of rats treated with saline or SKF 83959. Pretreatment with TAT-D1 led to decreased co-immunoprecipitated receptors. An aliquot of each sample was used as a control for WB (input control). (E,F) The CPA induced by D1-D2 heteromer stimulation was abolished by Cdk5 inhibitor roscovitine pre-treatment (200 nmol, i.c.v, E ) or intra-accumbal injection (30 nmol, F ). (G) Representative western blot and histogram showing the density of Thr75-DARPP-32 phosphorylation (pT75) relative to GAPDH (as loading control). Data represent means ± SEM of n = 8–10 rats/group. ( * p
    Figure Legend Snippet: The effects of D1-D2 heteromer stimulation and inactivation on basal conditioned place preference. (A) Vehicle-conditioned rats did not exhibit a preference toward a particular chamber. D1-D2 heteromer stimulation by SKF 83959 (1.5 mg/kg, s.c.) induced conditioned place aversion (CPA) as the animals spent significantly less time in the drug paired chamber. (B) SKF 83959-induced CPA was abolished by pre-treatment by the D1-D2 heteromer selective disrupting peptide, TAT-D1, but not the control TAT-Sc peptide. (C) Inactivation of D1-D2 heteromer by TAT-D1 resulted in conditioned place preference (CPP) as the rats spent significantly more time in the drug paired chamber, not observed with the control TAT-Sc. (D) Representative western blots (inset) and histogram showing the amount of D1R co-immunoprecipitated with D2R from the NAc of rats treated with saline or SKF 83959. Pretreatment with TAT-D1 led to decreased co-immunoprecipitated receptors. An aliquot of each sample was used as a control for WB (input control). (E,F) The CPA induced by D1-D2 heteromer stimulation was abolished by Cdk5 inhibitor roscovitine pre-treatment (200 nmol, i.c.v, E ) or intra-accumbal injection (30 nmol, F ). (G) Representative western blot and histogram showing the density of Thr75-DARPP-32 phosphorylation (pT75) relative to GAPDH (as loading control). Data represent means ± SEM of n = 8–10 rats/group. ( * p

    Techniques Used: Conditioned Place Preference, Western Blot, Immunoprecipitation, Injection

    Evidence for the existence of dopamine D1-D2 receptor heteromer in rat NAc. (A) Proximity ligation assay (PLA) was used to visualize and detect D1R and D2R close proximity. (A1) A scheme depicts the PLA probes used in the present study. (A2–A4) Representative images of PLA signals (red dots) in neurons (nuclei stained by DAPI) in rat caudate putamen (CPu), nucleus accumbens core (NAc-core) and shell (NAc-shell) subregions. (A5) Graph representing the percent of neurons with a positive PLA signal. (A6) Representative image of PLA signals in neurons (nuclei stained by DAPI) in rat NAc-core using the second set of antibodies. (B) Representative images of immunohistochemistry using D1R antibody (D1R-Ab) or D2R antibody (D2R-Ab) directly conjugated to Alexa-488 or Alexa-568, respectively, in the NAc-shell. Direct confocal FRET analysis was performed, reflected by FRET efficiency (FRET E) and the distance between the dipoles, less than 10 nm (100 Å). (C) A representative close-up of a single MSN cell body from NAc showing D1R-D2R colocalization (left), D1-D2 heteromer FRET efficiency (center) and relative distance between receptors (right). (D,E) Histograms showing FRET E ratios (D) and distance (E) obtained from MSN cell bodies from NAc ( n = 24). Bars are 10 μm.
    Figure Legend Snippet: Evidence for the existence of dopamine D1-D2 receptor heteromer in rat NAc. (A) Proximity ligation assay (PLA) was used to visualize and detect D1R and D2R close proximity. (A1) A scheme depicts the PLA probes used in the present study. (A2–A4) Representative images of PLA signals (red dots) in neurons (nuclei stained by DAPI) in rat caudate putamen (CPu), nucleus accumbens core (NAc-core) and shell (NAc-shell) subregions. (A5) Graph representing the percent of neurons with a positive PLA signal. (A6) Representative image of PLA signals in neurons (nuclei stained by DAPI) in rat NAc-core using the second set of antibodies. (B) Representative images of immunohistochemistry using D1R antibody (D1R-Ab) or D2R antibody (D2R-Ab) directly conjugated to Alexa-488 or Alexa-568, respectively, in the NAc-shell. Direct confocal FRET analysis was performed, reflected by FRET efficiency (FRET E) and the distance between the dipoles, less than 10 nm (100 Å). (C) A representative close-up of a single MSN cell body from NAc showing D1R-D2R colocalization (left), D1-D2 heteromer FRET efficiency (center) and relative distance between receptors (right). (D,E) Histograms showing FRET E ratios (D) and distance (E) obtained from MSN cell bodies from NAc ( n = 24). Bars are 10 μm.

    Techniques Used: Proximity Ligation Assay, Staining, Immunohistochemistry

    3) Product Images from "Activation of Dopamine D1-D2 Receptor Complex Attenuates Cocaine Reward and Reinstatement of Cocaine-Seeking through Inhibition of DARPP-32, ERK, and ΔFosB"

    Article Title: Activation of Dopamine D1-D2 Receptor Complex Attenuates Cocaine Reward and Reinstatement of Cocaine-Seeking through Inhibition of DARPP-32, ERK, and ΔFosB

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00924

    The effects of D1-D2 heteromer stimulation and inactivation on basal conditioned place preference. (A) Vehicle-conditioned rats did not exhibit a preference toward a particular chamber. D1-D2 heteromer stimulation by SKF 83959 (1.5 mg/kg, s.c.) induced conditioned place aversion (CPA) as the animals spent significantly less time in the drug paired chamber. (B) SKF 83959-induced CPA was abolished by pre-treatment by the D1-D2 heteromer selective disrupting peptide, TAT-D1, but not the control TAT-Sc peptide. (C) Inactivation of D1-D2 heteromer by TAT-D1 resulted in conditioned place preference (CPP) as the rats spent significantly more time in the drug paired chamber, not observed with the control TAT-Sc. (D) Representative western blots (inset) and histogram showing the amount of D1R co-immunoprecipitated with D2R from the NAc of rats treated with saline or SKF 83959. Pretreatment with TAT-D1 led to decreased co-immunoprecipitated receptors. An aliquot of each sample was used as a control for WB (input control). (E,F) The CPA induced by D1-D2 heteromer stimulation was abolished by Cdk5 inhibitor roscovitine pre-treatment (200 nmol, i.c.v, E ) or intra-accumbal injection (30 nmol, F ). (G) Representative western blot and histogram showing the density of Thr75-DARPP-32 phosphorylation (pT75) relative to GAPDH (as loading control). Data represent means ± SEM of n = 8–10 rats/group. ( * p
    Figure Legend Snippet: The effects of D1-D2 heteromer stimulation and inactivation on basal conditioned place preference. (A) Vehicle-conditioned rats did not exhibit a preference toward a particular chamber. D1-D2 heteromer stimulation by SKF 83959 (1.5 mg/kg, s.c.) induced conditioned place aversion (CPA) as the animals spent significantly less time in the drug paired chamber. (B) SKF 83959-induced CPA was abolished by pre-treatment by the D1-D2 heteromer selective disrupting peptide, TAT-D1, but not the control TAT-Sc peptide. (C) Inactivation of D1-D2 heteromer by TAT-D1 resulted in conditioned place preference (CPP) as the rats spent significantly more time in the drug paired chamber, not observed with the control TAT-Sc. (D) Representative western blots (inset) and histogram showing the amount of D1R co-immunoprecipitated with D2R from the NAc of rats treated with saline or SKF 83959. Pretreatment with TAT-D1 led to decreased co-immunoprecipitated receptors. An aliquot of each sample was used as a control for WB (input control). (E,F) The CPA induced by D1-D2 heteromer stimulation was abolished by Cdk5 inhibitor roscovitine pre-treatment (200 nmol, i.c.v, E ) or intra-accumbal injection (30 nmol, F ). (G) Representative western blot and histogram showing the density of Thr75-DARPP-32 phosphorylation (pT75) relative to GAPDH (as loading control). Data represent means ± SEM of n = 8–10 rats/group. ( * p

    Techniques Used: Conditioned Place Preference, Western Blot, Immunoprecipitation, Injection

    Evidence for the existence of dopamine D1-D2 receptor heteromer in rat NAc. (A) Proximity ligation assay (PLA) was used to visualize and detect D1R and D2R close proximity. (A1) A scheme depicts the PLA probes used in the present study. (A2–A4) Representative images of PLA signals (red dots) in neurons (nuclei stained by DAPI) in rat caudate putamen (CPu), nucleus accumbens core (NAc-core) and shell (NAc-shell) subregions. (A5) Graph representing the percent of neurons with a positive PLA signal. (A6) Representative image of PLA signals in neurons (nuclei stained by DAPI) in rat NAc-core using the second set of antibodies. (B) Representative images of immunohistochemistry using D1R antibody (D1R-Ab) or D2R antibody (D2R-Ab) directly conjugated to Alexa-488 or Alexa-568, respectively, in the NAc-shell. Direct confocal FRET analysis was performed, reflected by FRET efficiency (FRET E) and the distance between the dipoles, less than 10 nm (100 Å). (C) A representative close-up of a single MSN cell body from NAc showing D1R-D2R colocalization (left), D1-D2 heteromer FRET efficiency (center) and relative distance between receptors (right). (D,E) Histograms showing FRET E ratios (D) and distance (E) obtained from MSN cell bodies from NAc ( n = 24). Bars are 10 μm.
    Figure Legend Snippet: Evidence for the existence of dopamine D1-D2 receptor heteromer in rat NAc. (A) Proximity ligation assay (PLA) was used to visualize and detect D1R and D2R close proximity. (A1) A scheme depicts the PLA probes used in the present study. (A2–A4) Representative images of PLA signals (red dots) in neurons (nuclei stained by DAPI) in rat caudate putamen (CPu), nucleus accumbens core (NAc-core) and shell (NAc-shell) subregions. (A5) Graph representing the percent of neurons with a positive PLA signal. (A6) Representative image of PLA signals in neurons (nuclei stained by DAPI) in rat NAc-core using the second set of antibodies. (B) Representative images of immunohistochemistry using D1R antibody (D1R-Ab) or D2R antibody (D2R-Ab) directly conjugated to Alexa-488 or Alexa-568, respectively, in the NAc-shell. Direct confocal FRET analysis was performed, reflected by FRET efficiency (FRET E) and the distance between the dipoles, less than 10 nm (100 Å). (C) A representative close-up of a single MSN cell body from NAc showing D1R-D2R colocalization (left), D1-D2 heteromer FRET efficiency (center) and relative distance between receptors (right). (D,E) Histograms showing FRET E ratios (D) and distance (E) obtained from MSN cell bodies from NAc ( n = 24). Bars are 10 μm.

    Techniques Used: Proximity Ligation Assay, Staining, Immunohistochemistry

    4) Product Images from "Central Thalamic Deep-Brain Stimulation Alters Striatal-Thalamic Connectivity in Cognitive Neural Behavior"

    Article Title: Central Thalamic Deep-Brain Stimulation Alters Striatal-Thalamic Connectivity in Cognitive Neural Behavior

    Journal: Frontiers in Neural Circuits

    doi: 10.3389/fncir.2015.00087

    Western blot protein analysis of cells excised individually from striatal and hippocampal tissues. (A) The SDS-PAGE blots show the expression of dopamine D2 receptor (Drd2) and α4-nicotinic acetylcholine receptor (α4-nAChR) in the striatum (top) and hippocampus (bottom). (B) Results from the quantitative analysis of Drd2 and α4-nAChR expression (mean ± SEM; expressed as ratio to GAPDH) in the striatum (top) and hippocampus (bottom). Striatal and Drd2 and α4-nAChR protein expressions were significantly increased relative to sham control group. Significant increases in Drd2 and α4-nAChR protein expressions in the hippocampus also were found. * , ** , and *** indicate significant protein expression with P
    Figure Legend Snippet: Western blot protein analysis of cells excised individually from striatal and hippocampal tissues. (A) The SDS-PAGE blots show the expression of dopamine D2 receptor (Drd2) and α4-nicotinic acetylcholine receptor (α4-nAChR) in the striatum (top) and hippocampus (bottom). (B) Results from the quantitative analysis of Drd2 and α4-nAChR expression (mean ± SEM; expressed as ratio to GAPDH) in the striatum (top) and hippocampus (bottom). Striatal and Drd2 and α4-nAChR protein expressions were significantly increased relative to sham control group. Significant increases in Drd2 and α4-nAChR protein expressions in the hippocampus also were found. * , ** , and *** indicate significant protein expression with P

    Techniques Used: Western Blot, SDS Page, Expressing

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  • 93
    Alomone Labs anti d2 dopamine receptor extracellular antibody
    Quantitative PCR and western blot analyses of DA and 5-HT transporters, and dopamine D1 and D2 receptors in the striatum. qPCR (A–D) and western blot (E–I) analyses showing that no significant differences in the expression levels of DA transporter (DAT: A,E,I ), 5-HT transporter (5HTT: B,F,I ), D1 receptor (D1R: C,G,I ), and <t>D2</t> receptor (D2R: D,H,I ) were observed between FFAR1+/+ and −/− mice. n = 6 (A) and 5 (B-D) each. In (I) , n = 4 (+/+) and 6 (−/−) for DAT, n = 8.
    Anti D2 Dopamine Receptor Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti d2 dopamine receptor extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti d2 dopamine receptor extracellular antibody - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

    93
    Alomone Labs anti d2 dopamine receptor fitc antibody solution
    Quantitative PCR and western blot analyses of DA and 5-HT transporters, and dopamine D1 and D2 receptors in the striatum. qPCR (A–D) and western blot (E–I) analyses showing that no significant differences in the expression levels of DA transporter (DAT: A,E,I ), 5-HT transporter (5HTT: B,F,I ), D1 receptor (D1R: C,G,I ), and <t>D2</t> receptor (D2R: D,H,I ) were observed between FFAR1+/+ and −/− mice. n = 6 (A) and 5 (B-D) each. In (I) , n = 4 (+/+) and 6 (−/−) for DAT, n = 8.
    Anti D2 Dopamine Receptor Fitc Antibody Solution, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti d2 dopamine receptor fitc antibody solution/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti d2 dopamine receptor fitc antibody solution - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

    Image Search Results


    Quantitative PCR and western blot analyses of DA and 5-HT transporters, and dopamine D1 and D2 receptors in the striatum. qPCR (A–D) and western blot (E–I) analyses showing that no significant differences in the expression levels of DA transporter (DAT: A,E,I ), 5-HT transporter (5HTT: B,F,I ), D1 receptor (D1R: C,G,I ), and D2 receptor (D2R: D,H,I ) were observed between FFAR1+/+ and −/− mice. n = 6 (A) and 5 (B-D) each. In (I) , n = 4 (+/+) and 6 (−/−) for DAT, n = 8.

    Journal: Frontiers in Pharmacology

    Article Title: FFAR1/GPR40 Contributes to the Regulation of Striatal Monoamine Releases and Facilitation of Cocaine-Induced Locomotor Activity in Mice

    doi: 10.3389/fphar.2021.699026

    Figure Lengend Snippet: Quantitative PCR and western blot analyses of DA and 5-HT transporters, and dopamine D1 and D2 receptors in the striatum. qPCR (A–D) and western blot (E–I) analyses showing that no significant differences in the expression levels of DA transporter (DAT: A,E,I ), 5-HT transporter (5HTT: B,F,I ), D1 receptor (D1R: C,G,I ), and D2 receptor (D2R: D,H,I ) were observed between FFAR1+/+ and −/− mice. n = 6 (A) and 5 (B-D) each. In (I) , n = 4 (+/+) and 6 (−/−) for DAT, n = 8.

    Article Snippet: For the anti-D1 and D2 receptor antibodies, the specificities were validated with D1 and D2 receptor deficient mice ( ).

    Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Mouse Assay