Journal: Cerebral Cortex (New York, NY)

Article Title: Dopamine Receptor Expression Among Local and Visual Cortex-Projecting Frontal Eye Field Neurons

doi: 10.1093/cercor/bhz078

Figure Lengend Snippet: Antibody information

Article Snippet: DAPI stains DNA and is therefore a label for all nucleated cells. table ft1 table-wrap mode="anchored" t5 Table 3 caption a7 Antigen Host Vendor information Dilution D1R Rabbit polyclonal ADR-001; Alomone Labs, Jerusalem, Israel 1:200 D2R Goat polyclonal SC-7522; Santa Cruz Biotechnology, Santa Cruz, CA 1:200 D2R Rabbit polyclonal ADR-002; Alomone Labs, Jerusalem, Israel 1:200 Neurogranin Mouse monoclonal SC-514922; Santa Cruz Biotechnology, Santa Cruz, CA 1:200 SMI-32 Mouse monoclonal NE1023; Millipore, Temecula, CA 1:1000 Parvalbumin Mouse monoclonal P3088; Sigma-Aldrich Inc., St. Lous, MO 1:1000 Calbindin Mouse monoclonal CB300; Swant Inc., Switzerland 1:1000 Calretinin Mouse monoclonal 6B3; Swant Inc., Switzerland 1:1000 Somatostatin Rat monoclonal MAB354; Millipore, Temecula, CA 1:400 Open in a separate window Antibody information

Techniques:

Journal: Biology of Sex Differences

Article Title: Sex difference in dopamine D1-D2 receptor complex expression and signaling affects depression- and anxiety-like behaviors

doi: 10.1186/s13293-020-00285-9

Figure Lengend Snippet: Comparison of the expression of dopamine D1-D2 receptor heteromer between male and female rats by co-immunoprecipitation (Co-IP) and proximity ligation assay (PLA). a Co-IP of D1R with anti-D2R antibody from NAc and CPu of male (M) and female (F) rats, followed by western blot to visualize D1R. b Western blot analysis of D1R in NAc and CPu in both sexes. c Western blot analysis of D2R in NAc and CPu of both sexes. d Quantification of Co-IP and WB results from a – c . N = 3–4 rats/group for Co-IP and N = 6 rats/group for WB. * p < 0 . 05 , ** p < 0.01 using t test. e Confocal images and insets (left) and quantification (right) of D1-D2 PLA signal in NAc and CPu of male and female rat. N = 6 rats/group, ANOVA, followed by Bonferroni’s post-test comparisons. ** p < 0 . 001 , *** p < 0 . 0001 . Data are expressed as means ± SEM. f Confocal images (left) and quantification (right) of D1-D2 PLA signal in the caudate and putamen nuclei of male and female African Green monkey. N = 3 animals/group. *** p < 0 . 0001 using t test. Data are expressed as means ± SEM

Article Snippet: Protein homogenates (250–300 μg) from rat NAc or CPu were incubated with an anti-D2R antibody (Alomone Laboratories) at 4 °C overnight under gentle rotation.

Techniques: Expressing, Immunoprecipitation, Co-Immunoprecipitation Assay, Proximity Ligation Assay, Western Blot

Journal: Pediatric research

Article Title: Role of dopamine and selective dopamine receptor agonists on mouse ductus arteriosus tone and responsiveness

doi: 10.1038/s41390-019-0716-x

Figure Lengend Snippet: (A) RT-qPCR indicates the presence of DA 1 and DA 2 dopamine receptors in the day 19 mouse embryo ductus arteriosus (d19 DA) compared to aorta (d19 Aorta) (15 representative offspring from 3 litters). Isolation and immunostaining of term gestation outflow tracts (B) showed localization of DA 1 receptor protein (DRD1) in the muscular media and adventitia (arrows) (C) and localization of DA 2 receptor protein (DRD2) in the intimal (endothelial) and adventitial layers (D) of the ductus (arrows). (E) The isolated ductus displayed significant dopamine-induced vasoconstriction compared to baseline diameter under deoxy (“fetal”; o; n=9) and 12% (“newborn”; ●; n=7) oxygen conditions. (F) Term vessels under newborn oxygen conditions were pretreated with different inhibitors: 10 −6 M SCH23390 (o; n=8), L-741,626 (□; n=9), or phentolamine (▲; n=9), compared to untreated vessels (12% O 2 ; ●; n=7). All pretreated vessels had significant change in ductus diameter at E max compared to resting baseline (BL) dimensions (*) and were significantly different from vessels that received no pretreatment († p < 0.0001, two-way ANOVA). Data shown are mean ± SEM; p < 0.05 (*) was considered significant. AO, aorta; BPA, branch pulmonary artery; DA ductus arteriosus; MPA, main pulmonary artery.

Article Snippet: The immunohistochemistry methodological approach for identifying the localization of proteins encoded by Drd1 using an anti-DA 1 -receptor antibody (DRD1, Alomone Labs, ADR-001) and Drd2 using an anti-DA 2 -receptor antibody (DRD2, Alomone Labs, ADR-002) was performed as we previously reported for serotonin receptors in the mouse ductus arteriosus ( ).

Techniques: Quantitative RT-PCR, Isolation, Immunostaining

Journal: Pediatric research

Article Title: Role of dopamine and selective dopamine receptor agonists on mouse ductus arteriosus tone and responsiveness

doi: 10.1038/s41390-019-0716-x

Figure Lengend Snippet: (A) RT-qPCR indicates the presence of DA 1 and DA 2 dopamine receptors in the day 19 mouse embryo ductus arteriosus (d19 DA) compared to aorta (d19 Aorta) (15 representative offspring from 3 litters). Isolation and immunostaining of term gestation outflow tracts (B) showed localization of DA 1 receptor protein (DRD1) in the muscular media and adventitia (arrows) (C) and localization of DA 2 receptor protein (DRD2) in the intimal (endothelial) and adventitial layers (D) of the ductus (arrows). (E) The isolated ductus displayed significant dopamine-induced vasoconstriction compared to baseline diameter under deoxy (“fetal”; o; n=9) and 12% (“newborn”; ●; n=7) oxygen conditions. (F) Term vessels under newborn oxygen conditions were pretreated with different inhibitors: 10 −6 M SCH23390 (o; n=8), L-741,626 (□; n=9), or phentolamine (▲; n=9), compared to untreated vessels (12% O 2 ; ●; n=7). All pretreated vessels had significant change in ductus diameter at E max compared to resting baseline (BL) dimensions (*) and were significantly different from vessels that received no pretreatment († p < 0.0001, two-way ANOVA). Data shown are mean ± SEM; p < 0.05 (*) was considered significant. AO, aorta; BPA, branch pulmonary artery; DA ductus arteriosus; MPA, main pulmonary artery.

Article Snippet: The immunohistochemistry methodological approach for identifying the localization of proteins encoded by Drd1 using an anti-DA 1 -receptor antibody (DRD1, Alomone Labs, ADR-001) and Drd2 using an anti-DA 2 -receptor antibody (DRD2, Alomone Labs, ADR-002) was performed as we previously reported for serotonin receptors in the mouse ductus arteriosus ( ).

Techniques: Quantitative RT-PCR, Isolation, Immunostaining