yeast adh (Worthington Biochemical)


Structured Review
Yeast Adh, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/yeast adh/product/Worthington Biochemical
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
yeast adh (Worthington Biochemical)


Structured Review
Yeast Adh, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/yeast adh/product/Worthington Biochemical
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
yeast adh (Worthington Biochemical)


Structured Review
Yeast Adh, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/yeast adh/product/Worthington Biochemical
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
adh (Worthington Biochemical)


Structured Review
Adh, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adh/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
alcohol dehydrogenase adh (Worthington Biochemical)


Structured Review
Alcohol Dehydrogenase Adh, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alcohol dehydrogenase adh/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
alcohol dehydrogenase adh (Worthington Biochemical)


Structured Review
Alcohol Dehydrogenase Adh, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alcohol dehydrogenase adh/product/Worthington Biochemical
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
adh assay (Worthington Biochemical)


Structured Review
Adh Assay, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adh assay/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
adh assay (Worthington Biochemical)


Structured Review

Adh Assay, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adh assay/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Proteomic Analysis of Protease Resistant Proteins in the Diabetic Rat Kidney "
Article Title: Proteomic Analysis of Protease Resistant Proteins in the Diabetic Rat Kidney
Journal: Molecular & Cellular Proteomics : MCP
doi: 10.1074/mcp.M112.020651

Figure Legend Snippet: Validation of LC-MSE identified PRPs by Western blotting. Protein lysate of Control (CK) and diabetic (DK) rat kidney was digested with trypsin, and separated on SDS-PAGE, followed by immunoblotting with antibodies against GAPDH, HSP 60, SOD, GST, LDH, and ADH. Presence of these proteins in DK after trypsin digestion confirmed the identification of PRPs by mass spectrometry. The experiment was repeated independently three times.
Techniques Used: Western Blot, SDS Page, Mass Spectrometry

Figure Legend Snippet: Functional assays of identified PRPs. The enzymatic activities of A, SOD, B, LDH, C, ADH, and D, GST were analyzed for control (CK) and diabetic (DK) rat kidney protein lysate using protocols described in supplemental method S1. In DK, significant reduction in enzymatic activity was observed. Bars represent means ± S.E. from three independent experiments. *p < 0.05.
Techniques Used: Functional Assay, Activity Assay

Figure Legend Snippet: Prediction of aggregation prone regions. Protein structures of rat GAPDH, HSP 60, SOD, GST, LDH and ADH were modeled by CPH 3.0 model server and analyzed using PyMol. Aggregation prone regions in these proteins were predicted in silico using Aggrescan, Tango, PASTA, and Waltz web servers (blue color). The regions common to aggregation prone sequences and glycation sites were highlighted in the red color, indicating that most of the glycation sites were overlapping with predicted aggregation hotspots.
Techniques Used: In Silico

Figure Legend Snippet: Expression analysis of PRPs. A, Protein expression of GAPDH, HSP 60, SOD, GST, LDH, and ADH in control (CK) and diabetic (DK) rat kidney was measured by the Western blot analysis. B, The same proteins were analyzed at the transcript level by performing semi-quantitative RT-PCR using total RNA isolated from control and diabetic rat kidney tissues. All samples were analyzed on 2% agarose gels containing gel red. Both analyses showed up-regulation of PRPs in diabetic condition. Results shown are representative of three independent experiments.
Techniques Used: Expressing, Western Blot, Quantitative RT-PCR, Isolation
adh assay (Worthington Biochemical)


Structured Review

Adh Assay, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adh assay/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Proteomic Analysis of Protease Resistant Proteins in the Diabetic Rat Kidney "
Article Title: Proteomic Analysis of Protease Resistant Proteins in the Diabetic Rat Kidney
Journal: Molecular & Cellular Proteomics : MCP
doi: 10.1074/mcp.M112.020651

Figure Legend Snippet: Validation of LC-MSE identified PRPs by Western blotting. Protein lysate of Control (CK) and diabetic (DK) rat kidney was digested with trypsin, and separated on SDS-PAGE, followed by immunoblotting with antibodies against GAPDH, HSP 60, SOD, GST, LDH, and ADH. Presence of these proteins in DK after trypsin digestion confirmed the identification of PRPs by mass spectrometry. The experiment was repeated independently three times.
Techniques Used: Western Blot, SDS Page, Mass Spectrometry

Figure Legend Snippet: Functional assays of identified PRPs. The enzymatic activities of A, SOD, B, LDH, C, ADH, and D, GST were analyzed for control (CK) and diabetic (DK) rat kidney protein lysate using protocols described in supplemental method S1. In DK, significant reduction in enzymatic activity was observed. Bars represent means ± S.E. from three independent experiments. *p < 0.05.
Techniques Used: Functional Assay, Activity Assay

Figure Legend Snippet: Prediction of aggregation prone regions. Protein structures of rat GAPDH, HSP 60, SOD, GST, LDH and ADH were modeled by CPH 3.0 model server and analyzed using PyMol. Aggregation prone regions in these proteins were predicted in silico using Aggrescan, Tango, PASTA, and Waltz web servers (blue color). The regions common to aggregation prone sequences and glycation sites were highlighted in the red color, indicating that most of the glycation sites were overlapping with predicted aggregation hotspots.
Techniques Used: In Silico

Figure Legend Snippet: Expression analysis of PRPs. A, Protein expression of GAPDH, HSP 60, SOD, GST, LDH, and ADH in control (CK) and diabetic (DK) rat kidney was measured by the Western blot analysis. B, The same proteins were analyzed at the transcript level by performing semi-quantitative RT-PCR using total RNA isolated from control and diabetic rat kidney tissues. All samples were analyzed on 2% agarose gels containing gel red. Both analyses showed up-regulation of PRPs in diabetic condition. Results shown are representative of three independent experiments.
Techniques Used: Expressing, Western Blot, Quantitative RT-PCR, Isolation
adh assay (Worthington Biochemical)


Structured Review

Adh Assay, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adh assay/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Proteomic Analysis of Protease Resistant Proteins in the Diabetic Rat Kidney "
Article Title: Proteomic Analysis of Protease Resistant Proteins in the Diabetic Rat Kidney
Journal: Molecular & Cellular Proteomics : MCP
doi: 10.1074/mcp.M112.020651

Figure Legend Snippet: Validation of LC-MSE identified PRPs by Western blotting. Protein lysate of Control (CK) and diabetic (DK) rat kidney was digested with trypsin, and separated on SDS-PAGE, followed by immunoblotting with antibodies against GAPDH, HSP 60, SOD, GST, LDH, and ADH. Presence of these proteins in DK after trypsin digestion confirmed the identification of PRPs by mass spectrometry. The experiment was repeated independently three times.
Techniques Used: Western Blot, SDS Page, Mass Spectrometry

Figure Legend Snippet: Functional assays of identified PRPs. The enzymatic activities of A, SOD, B, LDH, C, ADH, and D, GST were analyzed for control (CK) and diabetic (DK) rat kidney protein lysate using protocols described in supplemental method S1. In DK, significant reduction in enzymatic activity was observed. Bars represent means ± S.E. from three independent experiments. *p < 0.05.
Techniques Used: Functional Assay, Activity Assay

Figure Legend Snippet: Prediction of aggregation prone regions. Protein structures of rat GAPDH, HSP 60, SOD, GST, LDH and ADH were modeled by CPH 3.0 model server and analyzed using PyMol. Aggregation prone regions in these proteins were predicted in silico using Aggrescan, Tango, PASTA, and Waltz web servers (blue color). The regions common to aggregation prone sequences and glycation sites were highlighted in the red color, indicating that most of the glycation sites were overlapping with predicted aggregation hotspots.
Techniques Used: In Silico

Figure Legend Snippet: Expression analysis of PRPs. A, Protein expression of GAPDH, HSP 60, SOD, GST, LDH, and ADH in control (CK) and diabetic (DK) rat kidney was measured by the Western blot analysis. B, The same proteins were analyzed at the transcript level by performing semi-quantitative RT-PCR using total RNA isolated from control and diabetic rat kidney tissues. All samples were analyzed on 2% agarose gels containing gel red. Both analyses showed up-regulation of PRPs in diabetic condition. Results shown are representative of three independent experiments.
Techniques Used: Expressing, Western Blot, Quantitative RT-PCR, Isolation
adh assay (Worthington Biochemical)


Structured Review

Adh Assay, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adh assay/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Proteomic Analysis of Protease Resistant Proteins in the Diabetic Rat Kidney "
Article Title: Proteomic Analysis of Protease Resistant Proteins in the Diabetic Rat Kidney
Journal: Molecular & Cellular Proteomics : MCP
doi: 10.1074/mcp.M112.020651

Figure Legend Snippet: Validation of LC-MSE identified PRPs by Western blotting. Protein lysate of Control (CK) and diabetic (DK) rat kidney was digested with trypsin, and separated on SDS-PAGE, followed by immunoblotting with antibodies against GAPDH, HSP 60, SOD, GST, LDH, and ADH. Presence of these proteins in DK after trypsin digestion confirmed the identification of PRPs by mass spectrometry. The experiment was repeated independently three times.
Techniques Used: Western Blot, SDS Page, Mass Spectrometry

Figure Legend Snippet: Functional assays of identified PRPs. The enzymatic activities of A, SOD, B, LDH, C, ADH, and D, GST were analyzed for control (CK) and diabetic (DK) rat kidney protein lysate using protocols described in supplemental method S1. In DK, significant reduction in enzymatic activity was observed. Bars represent means ± S.E. from three independent experiments. *p < 0.05.
Techniques Used: Functional Assay, Activity Assay

Figure Legend Snippet: Prediction of aggregation prone regions. Protein structures of rat GAPDH, HSP 60, SOD, GST, LDH and ADH were modeled by CPH 3.0 model server and analyzed using PyMol. Aggregation prone regions in these proteins were predicted in silico using Aggrescan, Tango, PASTA, and Waltz web servers (blue color). The regions common to aggregation prone sequences and glycation sites were highlighted in the red color, indicating that most of the glycation sites were overlapping with predicted aggregation hotspots.
Techniques Used: In Silico

Figure Legend Snippet: Expression analysis of PRPs. A, Protein expression of GAPDH, HSP 60, SOD, GST, LDH, and ADH in control (CK) and diabetic (DK) rat kidney was measured by the Western blot analysis. B, The same proteins were analyzed at the transcript level by performing semi-quantitative RT-PCR using total RNA isolated from control and diabetic rat kidney tissues. All samples were analyzed on 2% agarose gels containing gel red. Both analyses showed up-regulation of PRPs in diabetic condition. Results shown are representative of three independent experiments.
Techniques Used: Expressing, Western Blot, Quantitative RT-PCR, Isolation