adh (Worthington Biochemical)


Structured Review

Adh, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adh/product/Worthington Biochemical
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Structural and Functional Consequences of Chaperone Site Deletion in αA-Crystallin"
Article Title: Structural and Functional Consequences of Chaperone Site Deletion in αA-Crystallin
Journal: Biochimica et biophysica acta
doi: 10.1016/j.bbapap.2016.08.006

Figure Legend Snippet: Comparison of anti-aggregation activities of wild-type αA-crystallin, αAΔ70–76 and αAΔ70–88 using ADH, β L -crystallin and CS as substrates Aggregation of ADH (0.25 mg/ml) was induced at 37 °C by phosphate buffer containing 100 mM EDTA (pH 7.3). Aggregation assays with β L -crystallin (0.25 mg/ml PBS) were performed at 55 °C, and aggregation assays of the CS sample (0.075 mg/ml 40 mM HEPES-NaOH buffer, pH 7.3) were performed at 43 °C. The percentage of substrate proteins aggregated in the presence of various concentrations of wild-type and mutant proteins at the 45 min time point of assay was calculated and plotted. The aggregation of substrate protein in the absence of chaperone protein was considered 100% aggregation.
Techniques Used: Mutagenesis