hiv 1 group m consensus gp140 cfi  (Worthington Biochemical)


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    Name:
    Adenosine Deaminase
    Description:
    A chromatographically purified dialyzed lyophilized powder Prepared by a modification of the method of Pfrogner Arch Biochim Biophys 119 141 1967
    Catalog Number:
    ls009043
    Price:
    160
    Size:
    250 un
    Source:
    Calf Spleen
    Cas Number:
    9026.93.1
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    Structured Review

    Worthington Biochemical hiv 1 group m consensus gp140 cfi
    Characteristics of <t>anti–HIV-1</t> gp41 Env clonal lineages. (A) Analysis of the reactivity of inferred RUA, intermediates, and observed antibodies in clonal lineage 558 with HIV-1 rgp41, autologous Env <t>gp140,</t> and CON-S Env gp140. The RUA (triangle) and reverted intermediates 1, 6, and 9 (square boxes), as well as observed antibody H828-K616, were assayed for reactivity with rgp41, autologous 684–6 gp140, and the <t>group</t> M consensus gp140 CON-S by Luminex assays. (B) V H sequences of clonal lineages identified by 454 deep sequencing. Shown are the numbers of clonal sequences (on the y axis) with the percentage of nucleotide change from germline sequences (on the x axis) for each of HIV-1 Env-reactive clonal lineage m (indicated by clonal lineage number with number of sequences found in parentheses) identified by 454 deep sequencing from genomic DNA of B cells of acute HIV-1 subjects. Sequences with identical V, D, and J segment usage and identical V-D and D-J junctions were considered to be members of an individual B cell clone. Data are representative of three separate experiments.
    A chromatographically purified dialyzed lyophilized powder Prepared by a modification of the method of Pfrogner Arch Biochim Biophys 119 141 1967
    https://www.bioz.com/result/hiv 1 group m consensus gp140 cfi/product/Worthington Biochemical
    Average 85 stars, based on 372 article reviews
    Price from $9.99 to $1999.99
    hiv 1 group m consensus gp140 cfi - by Bioz Stars, 2020-08
    85/100 stars

    Images

    1) Product Images from "Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated"

    Article Title: Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20110363

    Characteristics of anti–HIV-1 gp41 Env clonal lineages. (A) Analysis of the reactivity of inferred RUA, intermediates, and observed antibodies in clonal lineage 558 with HIV-1 rgp41, autologous Env gp140, and CON-S Env gp140. The RUA (triangle) and reverted intermediates 1, 6, and 9 (square boxes), as well as observed antibody H828-K616, were assayed for reactivity with rgp41, autologous 684–6 gp140, and the group M consensus gp140 CON-S by Luminex assays. (B) V H sequences of clonal lineages identified by 454 deep sequencing. Shown are the numbers of clonal sequences (on the y axis) with the percentage of nucleotide change from germline sequences (on the x axis) for each of HIV-1 Env-reactive clonal lineage m (indicated by clonal lineage number with number of sequences found in parentheses) identified by 454 deep sequencing from genomic DNA of B cells of acute HIV-1 subjects. Sequences with identical V, D, and J segment usage and identical V-D and D-J junctions were considered to be members of an individual B cell clone. Data are representative of three separate experiments.
    Figure Legend Snippet: Characteristics of anti–HIV-1 gp41 Env clonal lineages. (A) Analysis of the reactivity of inferred RUA, intermediates, and observed antibodies in clonal lineage 558 with HIV-1 rgp41, autologous Env gp140, and CON-S Env gp140. The RUA (triangle) and reverted intermediates 1, 6, and 9 (square boxes), as well as observed antibody H828-K616, were assayed for reactivity with rgp41, autologous 684–6 gp140, and the group M consensus gp140 CON-S by Luminex assays. (B) V H sequences of clonal lineages identified by 454 deep sequencing. Shown are the numbers of clonal sequences (on the y axis) with the percentage of nucleotide change from germline sequences (on the x axis) for each of HIV-1 Env-reactive clonal lineage m (indicated by clonal lineage number with number of sequences found in parentheses) identified by 454 deep sequencing from genomic DNA of B cells of acute HIV-1 subjects. Sequences with identical V, D, and J segment usage and identical V-D and D-J junctions were considered to be members of an individual B cell clone. Data are representative of three separate experiments.

    Techniques Used: Luminex, Sequencing

    2) Product Images from "Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated"

    Article Title: Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20110363

    Characteristics of anti–HIV-1 gp41 Env clonal lineages. (A) Analysis of the reactivity of inferred RUA, intermediates, and observed antibodies in clonal lineage 558 with HIV-1 rgp41, autologous Env gp140, and CON-S Env gp140. The RUA (triangle) and reverted intermediates 1, 6, and 9 (square boxes), as well as observed antibody H828-K616, were assayed for reactivity with rgp41, autologous 684–6 gp140, and the group M consensus gp140 CON-S by Luminex assays. (B) V H sequences of clonal lineages identified by 454 deep sequencing. Shown are the numbers of clonal sequences (on the y axis) with the percentage of nucleotide change from germline sequences (on the x axis) for each of HIV-1 Env-reactive clonal lineage m (indicated by clonal lineage number with number of sequences found in parentheses) identified by 454 deep sequencing from genomic DNA of B cells of acute HIV-1 subjects. Sequences with identical V, D, and J segment usage and identical V-D and D-J junctions were considered to be members of an individual B cell clone. Data are representative of three separate experiments.
    Figure Legend Snippet: Characteristics of anti–HIV-1 gp41 Env clonal lineages. (A) Analysis of the reactivity of inferred RUA, intermediates, and observed antibodies in clonal lineage 558 with HIV-1 rgp41, autologous Env gp140, and CON-S Env gp140. The RUA (triangle) and reverted intermediates 1, 6, and 9 (square boxes), as well as observed antibody H828-K616, were assayed for reactivity with rgp41, autologous 684–6 gp140, and the group M consensus gp140 CON-S by Luminex assays. (B) V H sequences of clonal lineages identified by 454 deep sequencing. Shown are the numbers of clonal sequences (on the y axis) with the percentage of nucleotide change from germline sequences (on the x axis) for each of HIV-1 Env-reactive clonal lineage m (indicated by clonal lineage number with number of sequences found in parentheses) identified by 454 deep sequencing from genomic DNA of B cells of acute HIV-1 subjects. Sequences with identical V, D, and J segment usage and identical V-D and D-J junctions were considered to be members of an individual B cell clone. Data are representative of three separate experiments.

    Techniques Used: Luminex, Sequencing

    Plasma cell response in AHI. (A) Sorting of plasma cells from AHI subjects (681–7, 684–6, 001–4, 0689 and 065–0). Dot plots were gated on CD3 − CD14 − CD16 − CD235a − CD19 + B cells (cells were also gated as CD20 lo/− during sorting). Ovals designate the populations that were sorted as plasma cells (CD27 hi/+ and CD38 hi/+ ). Numbers indicate percentage of cells in the sort gate. LKP, leukapheresis cells. (B) Specificity of antibodies isolated from plasma cells from each subject, or from all five AHI subjects pooled together. Total numbers of antibodies were indicated at the center of each pie chart. Percentage of antibodies binding to gp41, gp120, AT-2 inactivated HIV-1 clade B virion, and non–HIV-1/nondefined antigens are indicated in different colors. (C and D) Frequency of somatic mutations in V H gene segments of antibodies isolated from all five AHI subjects or from individuals 14 d after the second immunization (42 d after first dose) or 14 d after the fourth immunization (182 d after first dose) with gp120/NefTat/AS01B (D).
    Figure Legend Snippet: Plasma cell response in AHI. (A) Sorting of plasma cells from AHI subjects (681–7, 684–6, 001–4, 0689 and 065–0). Dot plots were gated on CD3 − CD14 − CD16 − CD235a − CD19 + B cells (cells were also gated as CD20 lo/− during sorting). Ovals designate the populations that were sorted as plasma cells (CD27 hi/+ and CD38 hi/+ ). Numbers indicate percentage of cells in the sort gate. LKP, leukapheresis cells. (B) Specificity of antibodies isolated from plasma cells from each subject, or from all five AHI subjects pooled together. Total numbers of antibodies were indicated at the center of each pie chart. Percentage of antibodies binding to gp41, gp120, AT-2 inactivated HIV-1 clade B virion, and non–HIV-1/nondefined antigens are indicated in different colors. (C and D) Frequency of somatic mutations in V H gene segments of antibodies isolated from all five AHI subjects or from individuals 14 d after the second immunization (42 d after first dose) or 14 d after the fourth immunization (182 d after first dose) with gp120/NefTat/AS01B (D).

    Techniques Used: Isolation, Binding Assay

    Related Articles

    Enzymatic Assay:

    Article Title: "REVERSE" CARBOCYCLIC FLEXIMERS: SYNTHESIS OF A NEW CLASS OF ADENOSINE DEAMINASE INHIBITORS
    Article Snippet: .. Enzyme assay solution typically contained 50 mM potassium phosphate at pH 7.4, 0.39 units of adenosine deaminase (Worthington Biochemical Corp., Lakewood, NJ, USA), 0.132 nM of SAHase (provided by Dr. Lynne Howell), 10 μM of SAH and various concentrations of inhibitors in a total volume of 1000 μL. ..

    other:

    Article Title: GPCR Ligand Dendrimer (GLiDe) Conjugates: Adenosine Receptor Interactions of a Series of Multivalent Xanthine Antagonists
    Article Snippet: Adenosine deaminase (25.3 U/mg) was purchased from Worthington Biochemical Corporation (Lakewood, NJ.)

    Article Title: Multi-Class Carcinogenic DNA Adduct Quantification in Formalin-Fixed Paraffin-Embedded Tissues by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry
    Article Snippet: Phosphodiesterase I ( Crotalus adamanteus venom) and adenosine deaminase were purchased from Worthington Biochemicals Corp. (Newark, NJ).

    Article Title: Evidence that adenosine contributes to Leao’s spreading depression in vivo
    Article Snippet: Lyophilized adenosine deaminase (ADA) from bovine spleen (lot X4C14821, Worthington Biochemical, Lakewood, NJ) was reconstituted in phosphate-buffered saline (in mM, NaCl 137, KCl 2.7, Na2 HPO4 10, KH2 PO4 1.8 (pH 7.4)) and used at 100 U/mL.

    Article Title: Real-time monitoring of extracellular adenosine using enzyme-linked microelectrode arrays
    Article Snippet: The distal recording sites were coated with an ADA enzyme solution that consisted of 1% bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO), 0.125% glutaraldehyde (Sigma-Aldrich), 0.5 units of xanthine oxidase (XO) (Sigma-Aldrich) and 0.5 units of nucleoside phosphorylase (NP) (Sigma-Aldrich), and 2 units of adenosine deaminase (ADA) (Worthington Biochem, Lakewood, NJ).

    Article Title: Allosteric Antagonism of the A2A Adenosine Receptor by a Series of Bitopic Ligands
    Article Snippet: Adenosine deaminase was from Worthington Biochemical Corp. (Lakewood, NJ, USA).

    Incubation:

    Article Title: N6-Substituted-5′-N-Methylcarbamoyl-4′-selenoadenosines as Potent and Selective A3 Adenosine Receptor Agonists with Unusual Sugar Puckering and Nucleobase Orientation
    Article Snippet: .. The resultant pellets were resuspended in buffer containing 3 Units/mL adenosine deaminase (from bovine spleen, Worthington Biochemical Corp., Lakewood, NJ) and incubated at 37 °C for 30 min. .. The aliquots of membrane preparation were stored at -80 °C until the binding experiments.

    Cycling Probe Technology:

    Article Title: Evidence for constitutively-active adenosine receptors at mammalian motor nerve endings
    Article Snippet: .. 8-cyclopentyl-1,3,dipropylxanthine (CPX); 8-cyclopentyltheophylline (CPT) and (+)-tubocurarine chloride were obtained from Sigma-Aldrich, St Louis, MO, USA; adenosine deaminase was obtained from Worthington Biochemical Corporation, Freehold, NJ, USA. ..

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