cardiomyocytes (Worthington Biochemical)

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Neonatal Cardiomyocyte Isolation System

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Catalog Number:

LK003300

Price:

256

Size:

1 kt

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Worthington Biochemical cardiomyocytes

SUMO-1 overexpression protects SERCA2a activity from oxidative stress. (A) <t>Cardiomyocytes</t> were infected with adenoviruses carrying SUMO-1 or β-gal genes for 24 h and then, the cells were exposed to 0.5 μ M of H 2 O 2 for 6 h. SERCA2a SUMOylation was determined by IP with anti-SUMO-1 followed by WB with anti-SERCA2a antibody. Tyrosine nitration of SERCA2a was measured by IP with anti-SERCA2a followed by WB with anti-3-nitrotyrosine antibody. The cell extracts were subjected to ATPase activity assay of SERCA2a. (B) Cardiac-specific Nox4 knockout mice were subjected to TAC operation for 4 weeks. The expression levels of SERCA2a, SUMO-1, and SUMOylated SERCA2a in cardiac tissues of Nox4 knockout mice are shown. Data indicate the mean±SD of three independent experiments. * p

Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
https://www.bioz.com/result/cardiomyocytes/product/Worthington Biochemical
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99

cardiomyocytes - by Bioz Stars, 2021-04

98/100 stars

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1) Product Images from "The Role of SUMO-1 in Cardiac Oxidative Stress and Hypertrophy"

Article Title: The Role of SUMO-1 in Cardiac Oxidative Stress and Hypertrophy

Journal: Antioxidants & Redox Signaling

doi: 10.1089/ars.2014.5983

SUMO-1 overexpression protects SERCA2a activity from oxidative stress. (A) Cardiomyocytes were infected with adenoviruses carrying SUMO-1 or β-gal genes for 24 h and then, the cells were exposed to 0.5 μ M of H 2 O 2 for 6 h. SERCA2a SUMOylation was determined by IP with anti-SUMO-1 followed by WB with anti-SERCA2a antibody. Tyrosine nitration of SERCA2a was measured by IP with anti-SERCA2a followed by WB with anti-3-nitrotyrosine antibody. The cell extracts were subjected to ATPase activity assay of SERCA2a. (B) Cardiac-specific Nox4 knockout mice were subjected to TAC operation for 4 weeks. The expression levels of SERCA2a, SUMO-1, and SUMOylated SERCA2a in cardiac tissues of Nox4 knockout mice are shown. Data indicate the mean±SD of three independent experiments. * p

Figure Legend Snippet: SUMO-1 overexpression protects SERCA2a activity from oxidative stress. (A) Cardiomyocytes were infected with adenoviruses carrying SUMO-1 or β-gal genes for 24 h and then, the cells were exposed to 0.5 μ M of H 2 O 2 for 6 h. SERCA2a SUMOylation was determined by IP with anti-SUMO-1 followed by WB with anti-SERCA2a antibody. Tyrosine nitration of SERCA2a was measured by IP with anti-SERCA2a followed by WB with anti-3-nitrotyrosine antibody. The cell extracts were subjected to ATPase activity assay of SERCA2a. (B) Cardiac-specific Nox4 knockout mice were subjected to TAC operation for 4 weeks. The expression levels of SERCA2a, SUMO-1, and SUMOylated SERCA2a in cardiac tissues of Nox4 knockout mice are shown. Data indicate the mean±SD of three independent experiments. * p

Techniques Used: Over Expression, Activity Assay, Infection, Western Blot, Nitration, Knock-Out, Mouse Assay, Expressing

SUMO-1 overexpression inhibits PE-induced hypertrophic response in cardiomyocytes. (A) The neonatal cardiomyocytes were infected with the indicated adenoviruses and further stimulated with PE (100 μ M ) for 24 h. The relative expression levels of hypertrophic association genes such as ANF, BNP, and myh7 were determined by semi-quantitative RT-PCR ( n =3). (B) The protein synthesis rates after PE stimulation were evaluated by measuring 3 H-leucine incorporation. Data indicate the mean value±SD of three independent experiments. (C) Cell size measurement. (D) MAPK activities after the stimulation of neonatal cardiomyocytes with PE. Cardiomyocytes were infected with indicated adenoviruses for 24 h and then treated with PE (100 μ M ) for 24 h. Cell extracts were examined for amounts of phosphorylated ERK, p38, JNK, and c-Jun using phospho-specific antibodies. Total protein levels of ERK, p38, JNK, and c-Jun were also analyzed. The relative intensity was quantitated using ImageJ software. Data indicate the mean±SD of three independent experiments. # p

Figure Legend Snippet: SUMO-1 overexpression inhibits PE-induced hypertrophic response in cardiomyocytes. (A) The neonatal cardiomyocytes were infected with the indicated adenoviruses and further stimulated with PE (100 μ M ) for 24 h. The relative expression levels of hypertrophic association genes such as ANF, BNP, and myh7 were determined by semi-quantitative RT-PCR ( n =3). (B) The protein synthesis rates after PE stimulation were evaluated by measuring 3 H-leucine incorporation. Data indicate the mean value±SD of three independent experiments. (C) Cell size measurement. (D) MAPK activities after the stimulation of neonatal cardiomyocytes with PE. Cardiomyocytes were infected with indicated adenoviruses for 24 h and then treated with PE (100 μ M ) for 24 h. Cell extracts were examined for amounts of phosphorylated ERK, p38, JNK, and c-Jun using phospho-specific antibodies. Total protein levels of ERK, p38, JNK, and c-Jun were also analyzed. The relative intensity was quantitated using ImageJ software. Data indicate the mean±SD of three independent experiments. # p

Techniques Used: Over Expression, Infection, Expressing, Quantitative RT-PCR, Software

Isolation:

Article Title: Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study
Article Snippet: Molecular Probes (Eugene, OR) was the source of cholera toxin subunit B, horseradish peroxidase conjugate. .. The radiolabeled fatty acids [14 C]DHA and [3 H]OA were obtained from PerkinElmer (Boston, MA) The cardiomyocyte isolation kit was purchased from Worthington Biochemical (Lakewood, NJ). .. Horse and fetal bovine serums were from Hyclone (Logan, UT).

Article Title: Exercise induces new cardiomyocyte generation in the adult mammalian heart
Article Snippet: RNA of 1 μg was added to reverse transcription reactions (Applied Bioscience) and quantitative PCR was carried out using SYBR-green (Bio-Rad). .. Cardiomyocyte isolation and culture and gene expression Primary NRVMs were prepared from 1- to 2-day-old rats by use of the neonatal cardiomyocyte isolation system (Worthington Biochemical Corp.). .. Isolated NRVMs were purified by Percoll gradient centrifugation and plated in 60 mm dishes at 1 × 106 cells per well and cultured in DMEM/5% FBS/10% horse serum for 24 h. Before treatment, NRVM were synchronized, and cultured in 0.2% FBS DMEM media. miRNA-222 as well as control precursors (pre-miR) were purchased from Invitrogen.

Article Title: Delivery of an engineered HGF fragment in an extracellular matrix-derived hydrogel prevents negative LV remodeling post-myocardial infarction
Article Snippet: NP-40 lysis buffer was 20 mM Tris pH 8.0, 137 mM NaCl, 10% glycerol, and 1% IGEPAL/NP40. .. Neonatal cardiomyocytes were isolated from 1 to 2 day old Sprague-Dawley rats using the Neonatal Cardiomyocyte Isolation System (Worthington Biomedical Corporation, Lakewood, NJ). .. The cardiomyocytes were cultured in DMEM, 10% fetal bovine serum (Hyclone), and 0.5% Penicillin/Streptomycin (P/S, Invitrogen, Carlsbad, CA) antibiotic.

Article Title: Cardiac Niche Influences the Direct Reprogramming of Canine Fibroblasts into Cardiomyocyte-Like Cells
Article Snippet: .. Neonatal rat cardiomyocytes were isolated using the Neonatal Cardiomyocyte isolation kit from Worthington Biochemical Corporation (Lakewood, NJ, USA) and cultured in DMEM 10% FBS (Invitrogen, Carlsbad, CA, USA). ..

Article Title: Overexpression of miR-223 Tips the Balance of Pro- and Anti-hypertrophic Signaling Cascades toward Physiologic Cardiac Hypertrophy *
Article Snippet: The primer sequences of these genes are attached in . .. Hearts of rat neonates (1–3 days old) were removed after anesthetizing by ice-water bath, and cardiomyocytes were isolated using the Worthington Neonatal Cardiomyocytes Isolation System (Worthington Biochemical Corp., Lakewood, NJ) following the manufacturer's protocol. .. Neonatal cardiomyocytes were then cultured in DMEM medium supplemented with 2% fetal bovine serum (FBS) and 1% antibiotics (penicillin and streptomycin).

Article Title: Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R
Article Snippet: .. NRCMs were isolated using the Worthington Neonatal Cardiomyocytes Isolation System (Worthington Biochemical) according to the manufacturer’s procedures. ..

Article Title: Heart Failure and MEF2 Transcriptome Dynamics in Response to β-Blockers
Article Snippet: .. Primary cardiomyocyte isolation Primary neonatal rat cardiomyocytes were prepared from 1- to 3-day old rats (Sprague Dawley) using the Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corp). ..

Expressing:

Cell Culture:

other:

Article Title: Radixin Relocalization and Nonmuscle α-Actinin Expression Are Features of Remodeling Cardiomyocytes in Adult Patients with Dilated Cardiomyopathy
Article Snippet: Neonatal cardiomyocytes were plated at a density of 1 × 104 cells/cm2 and adult cardiomyocytes at 0.5 (low density) or at 1.5 × 104 cells/cm2 (high density) as indicated in the figures.