cardiomyocytes  (Worthington Biochemical)


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    Name:
    Neonatal Cardiomyocyte Isolation System
    Description:
    Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
    Catalog Number:
    LK003300
    Price:
    256
    Size:
    1 kt
    Cas Number:
    see components
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    Structured Review

    Worthington Biochemical cardiomyocytes
    SUMO-1 overexpression protects SERCA2a activity from oxidative stress. (A) <t>Cardiomyocytes</t> were infected with adenoviruses carrying SUMO-1 or β-gal genes for 24 h and then, the cells were exposed to 0.5 μ M of H 2 O 2 for 6 h. SERCA2a SUMOylation was determined by IP with anti-SUMO-1 followed by WB with anti-SERCA2a antibody. Tyrosine nitration of SERCA2a was measured by IP with anti-SERCA2a followed by WB with anti-3-nitrotyrosine antibody. The cell extracts were subjected to ATPase activity assay of SERCA2a. (B) Cardiac-specific Nox4 knockout mice were subjected to TAC operation for 4 weeks. The expression levels of SERCA2a, SUMO-1, and SUMOylated SERCA2a in cardiac tissues of Nox4 knockout mice are shown. Data indicate the mean±SD of three independent experiments. * p
    Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
    https://www.bioz.com/result/cardiomyocytes/product/Worthington Biochemical
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cardiomyocytes - by Bioz Stars, 2021-04
    98/100 stars

    Images

    1) Product Images from "The Role of SUMO-1 in Cardiac Oxidative Stress and Hypertrophy"

    Article Title: The Role of SUMO-1 in Cardiac Oxidative Stress and Hypertrophy

    Journal: Antioxidants & Redox Signaling

    doi: 10.1089/ars.2014.5983

    SUMO-1 overexpression protects SERCA2a activity from oxidative stress. (A) Cardiomyocytes were infected with adenoviruses carrying SUMO-1 or β-gal genes for 24 h and then, the cells were exposed to 0.5 μ M of H 2 O 2 for 6 h. SERCA2a SUMOylation was determined by IP with anti-SUMO-1 followed by WB with anti-SERCA2a antibody. Tyrosine nitration of SERCA2a was measured by IP with anti-SERCA2a followed by WB with anti-3-nitrotyrosine antibody. The cell extracts were subjected to ATPase activity assay of SERCA2a. (B) Cardiac-specific Nox4 knockout mice were subjected to TAC operation for 4 weeks. The expression levels of SERCA2a, SUMO-1, and SUMOylated SERCA2a in cardiac tissues of Nox4 knockout mice are shown. Data indicate the mean±SD of three independent experiments. * p
    Figure Legend Snippet: SUMO-1 overexpression protects SERCA2a activity from oxidative stress. (A) Cardiomyocytes were infected with adenoviruses carrying SUMO-1 or β-gal genes for 24 h and then, the cells were exposed to 0.5 μ M of H 2 O 2 for 6 h. SERCA2a SUMOylation was determined by IP with anti-SUMO-1 followed by WB with anti-SERCA2a antibody. Tyrosine nitration of SERCA2a was measured by IP with anti-SERCA2a followed by WB with anti-3-nitrotyrosine antibody. The cell extracts were subjected to ATPase activity assay of SERCA2a. (B) Cardiac-specific Nox4 knockout mice were subjected to TAC operation for 4 weeks. The expression levels of SERCA2a, SUMO-1, and SUMOylated SERCA2a in cardiac tissues of Nox4 knockout mice are shown. Data indicate the mean±SD of three independent experiments. * p

    Techniques Used: Over Expression, Activity Assay, Infection, Western Blot, Nitration, Knock-Out, Mouse Assay, Expressing

    SUMO-1 overexpression inhibits PE-induced hypertrophic response in cardiomyocytes. (A) The neonatal cardiomyocytes were infected with the indicated adenoviruses and further stimulated with PE (100 μ M ) for 24 h. The relative expression levels of hypertrophic association genes such as ANF, BNP, and myh7 were determined by semi-quantitative RT-PCR ( n =3). (B) The protein synthesis rates after PE stimulation were evaluated by measuring 3 H-leucine incorporation. Data indicate the mean value±SD of three independent experiments. (C) Cell size measurement. (D) MAPK activities after the stimulation of neonatal cardiomyocytes with PE. Cardiomyocytes were infected with indicated adenoviruses for 24 h and then treated with PE (100 μ M ) for 24 h. Cell extracts were examined for amounts of phosphorylated ERK, p38, JNK, and c-Jun using phospho-specific antibodies. Total protein levels of ERK, p38, JNK, and c-Jun were also analyzed. The relative intensity was quantitated using ImageJ software. Data indicate the mean±SD of three independent experiments. # p
    Figure Legend Snippet: SUMO-1 overexpression inhibits PE-induced hypertrophic response in cardiomyocytes. (A) The neonatal cardiomyocytes were infected with the indicated adenoviruses and further stimulated with PE (100 μ M ) for 24 h. The relative expression levels of hypertrophic association genes such as ANF, BNP, and myh7 were determined by semi-quantitative RT-PCR ( n =3). (B) The protein synthesis rates after PE stimulation were evaluated by measuring 3 H-leucine incorporation. Data indicate the mean value±SD of three independent experiments. (C) Cell size measurement. (D) MAPK activities after the stimulation of neonatal cardiomyocytes with PE. Cardiomyocytes were infected with indicated adenoviruses for 24 h and then treated with PE (100 μ M ) for 24 h. Cell extracts were examined for amounts of phosphorylated ERK, p38, JNK, and c-Jun using phospho-specific antibodies. Total protein levels of ERK, p38, JNK, and c-Jun were also analyzed. The relative intensity was quantitated using ImageJ software. Data indicate the mean±SD of three independent experiments. # p

    Techniques Used: Over Expression, Infection, Expressing, Quantitative RT-PCR, Software

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    Article Snippet: Molecular Probes (Eugene, OR) was the source of cholera toxin subunit B, horseradish peroxidase conjugate. .. The radiolabeled fatty acids [14 C]DHA and [3 H]OA were obtained from PerkinElmer (Boston, MA) The cardiomyocyte isolation kit was purchased from Worthington Biochemical (Lakewood, NJ). .. Horse and fetal bovine serums were from Hyclone (Logan, UT).

    Article Title: Exercise induces new cardiomyocyte generation in the adult mammalian heart
    Article Snippet: RNA of 1 μg was added to reverse transcription reactions (Applied Bioscience) and quantitative PCR was carried out using SYBR-green (Bio-Rad). .. Cardiomyocyte isolation and culture and gene expression Primary NRVMs were prepared from 1- to 2-day-old rats by use of the neonatal cardiomyocyte isolation system (Worthington Biochemical Corp.). .. Isolated NRVMs were purified by Percoll gradient centrifugation and plated in 60 mm dishes at 1 × 106 cells per well and cultured in DMEM/5% FBS/10% horse serum for 24 h. Before treatment, NRVM were synchronized, and cultured in 0.2% FBS DMEM media. miRNA-222 as well as control precursors (pre-miR) were purchased from Invitrogen.

    Article Title: Delivery of an engineered HGF fragment in an extracellular matrix-derived hydrogel prevents negative LV remodeling post-myocardial infarction
    Article Snippet: NP-40 lysis buffer was 20 mM Tris pH 8.0, 137 mM NaCl, 10% glycerol, and 1% IGEPAL/NP40. .. Neonatal cardiomyocytes were isolated from 1 to 2 day old Sprague-Dawley rats using the Neonatal Cardiomyocyte Isolation System (Worthington Biomedical Corporation, Lakewood, NJ). .. The cardiomyocytes were cultured in DMEM, 10% fetal bovine serum (Hyclone), and 0.5% Penicillin/Streptomycin (P/S, Invitrogen, Carlsbad, CA) antibiotic.

    Article Title: Cardiac Niche Influences the Direct Reprogramming of Canine Fibroblasts into Cardiomyocyte-Like Cells
    Article Snippet: .. Neonatal rat cardiomyocytes were isolated using the Neonatal Cardiomyocyte isolation kit from Worthington Biochemical Corporation (Lakewood, NJ, USA) and cultured in DMEM 10% FBS (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Overexpression of miR-223 Tips the Balance of Pro- and Anti-hypertrophic Signaling Cascades toward Physiologic Cardiac Hypertrophy *
    Article Snippet: The primer sequences of these genes are attached in . .. Hearts of rat neonates (1–3 days old) were removed after anesthetizing by ice-water bath, and cardiomyocytes were isolated using the Worthington Neonatal Cardiomyocytes Isolation System (Worthington Biochemical Corp., Lakewood, NJ) following the manufacturer's protocol. .. Neonatal cardiomyocytes were then cultured in DMEM medium supplemented with 2% fetal bovine serum (FBS) and 1% antibiotics (penicillin and streptomycin).

    Article Title: Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R
    Article Snippet: .. NRCMs were isolated using the Worthington Neonatal Cardiomyocytes Isolation System (Worthington Biochemical) according to the manufacturer’s procedures. ..

    Article Title: Heart Failure and MEF2 Transcriptome Dynamics in Response to β-Blockers
    Article Snippet: .. Primary cardiomyocyte isolation Primary neonatal rat cardiomyocytes were prepared from 1- to 3-day old rats (Sprague Dawley) using the Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corp). ..

    Expressing:

    Article Title: Exercise induces new cardiomyocyte generation in the adult mammalian heart
    Article Snippet: RNA of 1 μg was added to reverse transcription reactions (Applied Bioscience) and quantitative PCR was carried out using SYBR-green (Bio-Rad). .. Cardiomyocyte isolation and culture and gene expression Primary NRVMs were prepared from 1- to 2-day-old rats by use of the neonatal cardiomyocyte isolation system (Worthington Biochemical Corp.). .. Isolated NRVMs were purified by Percoll gradient centrifugation and plated in 60 mm dishes at 1 × 106 cells per well and cultured in DMEM/5% FBS/10% horse serum for 24 h. Before treatment, NRVM were synchronized, and cultured in 0.2% FBS DMEM media. miRNA-222 as well as control precursors (pre-miR) were purchased from Invitrogen.

    Cell Culture:

    Article Title: Cardiac Niche Influences the Direct Reprogramming of Canine Fibroblasts into Cardiomyocyte-Like Cells
    Article Snippet: .. Neonatal rat cardiomyocytes were isolated using the Neonatal Cardiomyocyte isolation kit from Worthington Biochemical Corporation (Lakewood, NJ, USA) and cultured in DMEM 10% FBS (Invitrogen, Carlsbad, CA, USA). ..

    other:

    Article Title: Radixin Relocalization and Nonmuscle α-Actinin Expression Are Features of Remodeling Cardiomyocytes in Adult Patients with Dilated Cardiomyopathy
    Article Snippet: Neonatal cardiomyocytes were plated at a density of 1 × 104 cells/cm2 and adult cardiomyocytes at 0.5 (low density) or at 1.5 × 104 cells/cm2 (high density) as indicated in the figures.

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    Worthington Biochemical fluorescent activated cell sorting neurospheres
    Neurosphere differentiation and composition. ( A and B ) 10 days-post ES cell differentiation <t>neurospheres</t> were dissociated and cell composition was quantified using FACS. Plots of V3, V2a, and V1 interneurons and motor neurons from Sim1:Cre;R26/C:LSL:Tomato, Chx10:Cre;R26/C:LSL:Tomato, En1:Cre;R26/C:LSL:Tomato, and Hb9:GFP ES cell reporter lines, respectively are shown after differentiation with ( A ) 1000 nM and ( B ) 5 nM SAG. ( C ) Two Chx:10:Cre;R26/C:LSL:Tomato ES cell lines were differentiated 10 days under a range of SAG concentrations. Tomato+V2a neuron number was quantified using FACS. ( D ) Chx:10:Cre;R26/C:LSL:Tomato lines #1 and #2 were differentiated with 15 nM and 40 nM SAG, respectively. 5 μM DAPT was applied days 6–10. Standardized tomato + V2a neurons relative to no DAPT control. DOI: http://dx.doi.org/10.7554/eLife.21540.004
    Fluorescent Activated Cell Sorting Neurospheres, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Worthington Biochemical cerebellar glucose 6 phosphate dehydrogenase
    Glucose metabolism in Cu− cerebellum. Cerebellar concentrations of glucose ( n = 5) and <t>glucose-6-phosphate</t> (G6P) ( n = 5) were found to be higher in Cu− rats without a corresponding increase in serum glucose ( n = 5) (Panel A). Levels of cerebellar glucose transporters Glut1 and Glut3 were not altered in Cu− rats (−) compared to Cu+ controls (+) (Panel B; n = 4). Values are means ± SEM; * P
    Cerebellar Glucose 6 Phosphate Dehydrogenase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Worthington Biochemical α amylase inhibitory activity
    <t>α‐Amylase</t> inhibitory activities of butternut squash residues (seed and skin extract). Values are means of duplicate determinations
    α Amylase Inhibitory Activity, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Neurosphere differentiation and composition. ( A and B ) 10 days-post ES cell differentiation neurospheres were dissociated and cell composition was quantified using FACS. Plots of V3, V2a, and V1 interneurons and motor neurons from Sim1:Cre;R26/C:LSL:Tomato, Chx10:Cre;R26/C:LSL:Tomato, En1:Cre;R26/C:LSL:Tomato, and Hb9:GFP ES cell reporter lines, respectively are shown after differentiation with ( A ) 1000 nM and ( B ) 5 nM SAG. ( C ) Two Chx:10:Cre;R26/C:LSL:Tomato ES cell lines were differentiated 10 days under a range of SAG concentrations. Tomato+V2a neuron number was quantified using FACS. ( D ) Chx:10:Cre;R26/C:LSL:Tomato lines #1 and #2 were differentiated with 15 nM and 40 nM SAG, respectively. 5 μM DAPT was applied days 6–10. Standardized tomato + V2a neurons relative to no DAPT control. DOI: http://dx.doi.org/10.7554/eLife.21540.004

    Journal: eLife

    Article Title: Speed and segmentation control mechanisms characterized in rhythmically-active circuits created from spinal neurons produced from genetically-tagged embryonic stem cells

    doi: 10.7554/eLife.21540

    Figure Lengend Snippet: Neurosphere differentiation and composition. ( A and B ) 10 days-post ES cell differentiation neurospheres were dissociated and cell composition was quantified using FACS. Plots of V3, V2a, and V1 interneurons and motor neurons from Sim1:Cre;R26/C:LSL:Tomato, Chx10:Cre;R26/C:LSL:Tomato, En1:Cre;R26/C:LSL:Tomato, and Hb9:GFP ES cell reporter lines, respectively are shown after differentiation with ( A ) 1000 nM and ( B ) 5 nM SAG. ( C ) Two Chx:10:Cre;R26/C:LSL:Tomato ES cell lines were differentiated 10 days under a range of SAG concentrations. Tomato+V2a neuron number was quantified using FACS. ( D ) Chx:10:Cre;R26/C:LSL:Tomato lines #1 and #2 were differentiated with 15 nM and 40 nM SAG, respectively. 5 μM DAPT was applied days 6–10. Standardized tomato + V2a neurons relative to no DAPT control. DOI: http://dx.doi.org/10.7554/eLife.21540.004

    Article Snippet: Fluorescent-activated cell sorting Neurospheres, 6–11 days from ES cells, were dissociated (Papain, Worthington), and then counted with a BD FACScan to determine the percentage of neurons composing neurospheres at different SAG concentrations.

    Techniques: Cell Differentiation, FACS

    Glucose metabolism in Cu− cerebellum. Cerebellar concentrations of glucose ( n = 5) and glucose-6-phosphate (G6P) ( n = 5) were found to be higher in Cu− rats without a corresponding increase in serum glucose ( n = 5) (Panel A). Levels of cerebellar glucose transporters Glut1 and Glut3 were not altered in Cu− rats (−) compared to Cu+ controls (+) (Panel B; n = 4). Values are means ± SEM; * P

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    Article Title: Fructose-2,6-Bisphosphate Is Lower in Copper Deficient Rat Cerebellum Despite Higher Content of Phosphorylated AMP-Activated Protein Kinase

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    Figure Lengend Snippet: Glucose metabolism in Cu− cerebellum. Cerebellar concentrations of glucose ( n = 5) and glucose-6-phosphate (G6P) ( n = 5) were found to be higher in Cu− rats without a corresponding increase in serum glucose ( n = 5) (Panel A). Levels of cerebellar glucose transporters Glut1 and Glut3 were not altered in Cu− rats (−) compared to Cu+ controls (+) (Panel B; n = 4). Values are means ± SEM; * P

    Article Snippet: Cerebellar glucose 6-phosphate dehydrogenase was assayed in 50 mM Tris pH 7.8 containing 3 mM MgCl2 (Worthington Biochemical Corp. Manual).

    Techniques:

    α‐Amylase inhibitory activities of butternut squash residues (seed and skin extract). Values are means of duplicate determinations

    Journal: Food Science & Nutrition

    Article Title: Evaluation of bioactivity of butternut squash (Cucurbita moschata D.) seeds and skin. Evaluation of bioactivity of butternut squash (Cucurbita moschata D.) seeds and skin

    doi: 10.1002/fsn3.1602

    Figure Lengend Snippet: α‐Amylase inhibitory activities of butternut squash residues (seed and skin extract). Values are means of duplicate determinations

    Article Snippet: The α‐amylase inhibitory activity was expressed as percentage inhibition (Worthington, Weddell, & Neilson, ).

    Techniques: