acsf buffer  (Worthington Biochemical)


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    Name:
    Collagenase Type 4
    Description:
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    Catalog Number:
    ls004186
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical acsf buffer
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/acsf buffer/product/Worthington Biochemical
    Average 88 stars, based on 2115 article reviews
    Price from $9.99 to $1999.99
    acsf buffer - by Bioz Stars, 2020-08
    88/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Incubation:

    Article Title: Cyclic AMP-dependent protein kinase and Epac mediate cyclic AMP responses in pancreatic acini
    Article Snippet: .. The pancreas was minced in buffer containing 40 mM Tris (pH 7.4), 95 mM NaCl, 4.7 mM KCl, 0.6 mM MgCl2 , 1.3 mM CaCl2 , 1 mM NaH2 PO4 , 10 mM glucose, 2 mM glutamine, plus 0.1% BSA, 1 × MEM-nones-sential amino acids (GIBCO-BRL, San Jose, CA), and 50 U/ml of type-4 collagenase (Worthington, Freehold, NJ) and then incubated for 1 h at 37°C. .. The digest was filtered through a 300–400 μm mesh (Sefar American, Depew, NY) and distributed in a 24-well Falcon tissue culture plate (Becton Dickinson, Franklin Lakes, NJ).

    Article Title: Effects of the antifungal antibiotic clotrimazole on human cardiac repolarization potassium currents
    Article Snippet: .. After 15 min (5 min at a time in fresh solutions), the chunks were incubated for 50 min in a similar solution containing 150–200 U ml−1 collagenase (CLS II, Worthington Biochemical, Freehold, NJ, U.S.A.), 1.2 U ml−1 protease (type XXIV, Sigma Chemical, St Louis, MO, U.S.A.), and 1 mg ml−1 bovine serum albumin (Sigma). ..

    Article Title: P2X2 knockout mice and P2X2/P2X3 double knockout mice reveal a role for the P2X2 receptor subunit in mediating multiple sensory effects of ATP
    Article Snippet: .. Ganglia were desheathed, cut and incubated in 4 ml Ca2+ /Mg2+ -free Hanks' balanced salt solution with 10 m m Hepes buffer (pH 7.0) (HBSS) (Life Technologies) containing 1.5 mg ml−1 collagenase (Class-II; Worthington Biochemical Corporation, Reading, UK) and 6 mg ml−1 bovine serum albumin (Sigma) at 37°C for 40 min. Ganglia were then incubated with 4 ml HBSS containing 1 mg ml−1 trypsin (Sigma) at 37°C for 20 min. ..

    Purification:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Homogenization:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

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