acsf buffer  (Worthington Biochemical)


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    Name:
    Papain Dissociation System
    Description:
    Set of five single use vials of papain and five single use vials of DNase 100 ml of Earle s balanced salt solution EBSS and an inhibitor vial for use in the tissue dissociation method of Huettner J and Baughman R J Neuroscience 6 3044 1986 Use tested by Worthington using new born rat pup spinal cord The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0 3 0 4 cm3 each
    Catalog Number:
    lk003150
    Price:
    240
    Size:
    1 bx
    Cas Number:
    see components
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    Structured Review

    Worthington Biochemical acsf buffer
    Set of five single use vials of papain and five single use vials of DNase 100 ml of Earle s balanced salt solution EBSS and an inhibitor vial for use in the tissue dissociation method of Huettner J and Baughman R J Neuroscience 6 3044 1986 Use tested by Worthington using new born rat pup spinal cord The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0 3 0 4 cm3 each
    https://www.bioz.com/result/acsf buffer/product/Worthington Biochemical
    Average 88 stars, based on 2242 article reviews
    Price from $9.99 to $1999.99
    acsf buffer - by Bioz Stars, 2020-10
    88/100 stars

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    Related Articles

    Gel Permeation Chromatography:

    Article Title: Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
    Article Snippet: .. The embryoid bodies were dissociated with papain (Papain dissociation system, Worthington), and the GPC were cultured and expanded in 10 μg/mL poly-L-ornithine (Sigma)/1 μg/mL laminin (Invitrogen)-coated plates in AM supplemented with 20 ng/mL fibroblast growth factor 2 (Joint Protein Central) and 20 ng/mL epidermal growth factor (Peprotech). .. Astrocytes were differentiated from low-confluent GPC cultures in DMEM/F12 Glutamax (Thermo Fisher Scientific) supplemented with N2 and B27 (both from Thermo Fisher Scientific) and 10% fetal bovine serum (FBS, Omega Scientific).

    Cell Culture:

    Article Title: Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
    Article Snippet: .. The embryoid bodies were dissociated with papain (Papain dissociation system, Worthington), and the GPC were cultured and expanded in 10 μg/mL poly-L-ornithine (Sigma)/1 μg/mL laminin (Invitrogen)-coated plates in AM supplemented with 20 ng/mL fibroblast growth factor 2 (Joint Protein Central) and 20 ng/mL epidermal growth factor (Peprotech). .. Astrocytes were differentiated from low-confluent GPC cultures in DMEM/F12 Glutamax (Thermo Fisher Scientific) supplemented with N2 and B27 (both from Thermo Fisher Scientific) and 10% fetal bovine serum (FBS, Omega Scientific).

    Fluorescence:

    Article Title: SNAP23 is selectively expressed in airway secretory cells and mediates baseline and stimulated mucin secretion
    Article Snippet: .. Fluorescence-activated sorting of airway epithelial cells A Papain Dissociation System (Worthington Biochemical Corporation) was used to dissociate airway epithelial cells from the trachea as described [ , ]. .. Cells from multiple mice were pooled and incubated with labelled antibodies to epithelial cell adhesion molecule (EpCAM-APC; eBiosciences) diluted 1:50 in 2.5% FBS in PBS for 30 min on ice, then sorted using a FACS Aria flow cytometer (BD Bioscences).

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