cardiomyocytes (Worthington Biochemical)
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Worthington Biochemical
cardiomyocytes
Cardiomyocytes, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cardiomyocytes/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Cardiomyocytes, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cardiomyocytes/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cardiomyocytes - by Bioz Stars,
2021-07
86/100 stars
Images
1) Product Images from "The Role of SUMO-1 in Cardiac Oxidative Stress and Hypertrophy"
Article Title: The Role of SUMO-1 in Cardiac Oxidative Stress and Hypertrophy
Journal: Antioxidants & Redox Signaling
doi: 10.1089/ars.2014.5983
Figure Legend Snippet: SUMO-1 overexpression protects SERCA2a activity from oxidative stress. (A) Cardiomyocytes were infected with adenoviruses carrying SUMO-1 or β-gal genes for 24 h and then, the cells were exposed to 0.5 μ M of H 2 O 2 for 6 h. SERCA2a SUMOylation was determined by IP with anti-SUMO-1 followed by WB with anti-SERCA2a antibody. Tyrosine nitration of SERCA2a was measured by IP with anti-SERCA2a followed by WB with anti-3-nitrotyrosine antibody. The cell extracts were subjected to ATPase activity assay of SERCA2a. (B) Cardiac-specific Nox4 knockout mice were subjected to TAC operation for 4 weeks. The expression levels of SERCA2a, SUMO-1, and SUMOylated SERCA2a in cardiac tissues of Nox4 knockout mice are shown. Data indicate the mean±SD of three independent experiments. * p
Techniques Used: Over Expression, Activity Assay, Infection, Western Blot, Nitration, Knock-Out, Mouse Assay, Expressing
Figure Legend Snippet: SUMO-1 overexpression inhibits PE-induced hypertrophic response in cardiomyocytes. (A) The neonatal cardiomyocytes were infected with the indicated adenoviruses and further stimulated with PE (100 μ M ) for 24 h. The relative expression levels of hypertrophic association genes such as ANF, BNP, and myh7 were determined by semi-quantitative RT-PCR ( n =3). (B) The protein synthesis rates after PE stimulation were evaluated by measuring 3 H-leucine incorporation. Data indicate the mean value±SD of three independent experiments. (C) Cell size measurement. (D) MAPK activities after the stimulation of neonatal cardiomyocytes with PE. Cardiomyocytes were infected with indicated adenoviruses for 24 h and then treated with PE (100 μ M ) for 24 h. Cell extracts were examined for amounts of phosphorylated ERK, p38, JNK, and c-Jun using phospho-specific antibodies. Total protein levels of ERK, p38, JNK, and c-Jun were also analyzed. The relative intensity was quantitated using ImageJ software. Data indicate the mean±SD of three independent experiments. # p
Techniques Used: Over Expression, Infection, Expressing, Quantitative RT-PCR, Software
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