alomone  (Alomone Labs)


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    Alomone Labs alomone
    mCB 2 immunostaining in midbrain DA neurons using the <t>Alomone</t> rCB 2 antibody. a Representative IHC staining results illustrating that the Alomone rCB 2 antibody detected significantly lower CB 2 immunostaining in the Zimmer strain of CB 2 -KO mice. b , c Diagrams showing the receptor-deleted regions and the binding site of the Alomone rCB 2 antibody in both strains of CB 2 -KO mice. d The mean densities of mCB 2 staining in DA neurons (over 200–400 DA neurons from 2 to 3 mice per genotype) illustrating a significant reduction in mCB 2 immunostaining in the Zimmer and Deltagen CB 2 -KO mice compared to the other strains. *** P
    Alomone, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alomone/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alomone - by Bioz Stars, 2022-05
    93/100 stars

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    1) Product Images from "CB2 receptor antibody signal specificity: correlations with the use of partial CB2-knockout mice and anti-rat CB2 receptor antibodies"

    Article Title: CB2 receptor antibody signal specificity: correlations with the use of partial CB2-knockout mice and anti-rat CB2 receptor antibodies

    Journal: Acta Pharmacologica Sinica

    doi: 10.1038/s41401-018-0037-3

    mCB 2 immunostaining in midbrain DA neurons using the Alomone rCB 2 antibody. a Representative IHC staining results illustrating that the Alomone rCB 2 antibody detected significantly lower CB 2 immunostaining in the Zimmer strain of CB 2 -KO mice. b , c Diagrams showing the receptor-deleted regions and the binding site of the Alomone rCB 2 antibody in both strains of CB 2 -KO mice. d The mean densities of mCB 2 staining in DA neurons (over 200–400 DA neurons from 2 to 3 mice per genotype) illustrating a significant reduction in mCB 2 immunostaining in the Zimmer and Deltagen CB 2 -KO mice compared to the other strains. *** P
    Figure Legend Snippet: mCB 2 immunostaining in midbrain DA neurons using the Alomone rCB 2 antibody. a Representative IHC staining results illustrating that the Alomone rCB 2 antibody detected significantly lower CB 2 immunostaining in the Zimmer strain of CB 2 -KO mice. b , c Diagrams showing the receptor-deleted regions and the binding site of the Alomone rCB 2 antibody in both strains of CB 2 -KO mice. d The mean densities of mCB 2 staining in DA neurons (over 200–400 DA neurons from 2 to 3 mice per genotype) illustrating a significant reduction in mCB 2 immunostaining in the Zimmer and Deltagen CB 2 -KO mice compared to the other strains. *** P

    Techniques Used: Immunostaining, Immunohistochemistry, Staining, Mouse Assay, Binding Assay

    Western blot results in WT, CB 1 -KO, and Zimmer CB 2 -KO mice using four different CB 2 antibodies. a A diagram showing the binding sites of the four antibodies used in WB assays. b The Abcam rCB 2 -Ab detected an mCB 2 -like band at ~40 kD (mCB 2 monomer, 39 kD) in the striatum of all three mouse genotypes, but the other three antibodies (Alomone, NIH5633, Mackie) detected relatively lower densities of an mCB 2 -like band in Zimmer CB 2 -KO mice. c CB 2 -immunoreactive bands detected in the spleen using the same antibodies. More bands were detected in the striatum than the spleen by the multiple antibodies. Preabsorption of the CB 2 antibodies (Abcam, Alomone, NIH5633) with specific immune peptides blocked the immunoreactive bands
    Figure Legend Snippet: Western blot results in WT, CB 1 -KO, and Zimmer CB 2 -KO mice using four different CB 2 antibodies. a A diagram showing the binding sites of the four antibodies used in WB assays. b The Abcam rCB 2 -Ab detected an mCB 2 -like band at ~40 kD (mCB 2 monomer, 39 kD) in the striatum of all three mouse genotypes, but the other three antibodies (Alomone, NIH5633, Mackie) detected relatively lower densities of an mCB 2 -like band in Zimmer CB 2 -KO mice. c CB 2 -immunoreactive bands detected in the spleen using the same antibodies. More bands were detected in the striatum than the spleen by the multiple antibodies. Preabsorption of the CB 2 antibodies (Abcam, Alomone, NIH5633) with specific immune peptides blocked the immunoreactive bands

    Techniques Used: Western Blot, Mouse Assay, Binding Assay

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  • 93
    Alomone Labs alomone
    mCB 2 immunostaining in midbrain DA neurons using the <t>Alomone</t> rCB 2 antibody. a Representative IHC staining results illustrating that the Alomone rCB 2 antibody detected significantly lower CB 2 immunostaining in the Zimmer strain of CB 2 -KO mice. b , c Diagrams showing the receptor-deleted regions and the binding site of the Alomone rCB 2 antibody in both strains of CB 2 -KO mice. d The mean densities of mCB 2 staining in DA neurons (over 200–400 DA neurons from 2 to 3 mice per genotype) illustrating a significant reduction in mCB 2 immunostaining in the Zimmer and Deltagen CB 2 -KO mice compared to the other strains. *** P
    Alomone, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alomone/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alomone - by Bioz Stars, 2022-05
    93/100 stars
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    mCB 2 immunostaining in midbrain DA neurons using the Alomone rCB 2 antibody. a Representative IHC staining results illustrating that the Alomone rCB 2 antibody detected significantly lower CB 2 immunostaining in the Zimmer strain of CB 2 -KO mice. b , c Diagrams showing the receptor-deleted regions and the binding site of the Alomone rCB 2 antibody in both strains of CB 2 -KO mice. d The mean densities of mCB 2 staining in DA neurons (over 200–400 DA neurons from 2 to 3 mice per genotype) illustrating a significant reduction in mCB 2 immunostaining in the Zimmer and Deltagen CB 2 -KO mice compared to the other strains. *** P

    Journal: Acta Pharmacologica Sinica

    Article Title: CB2 receptor antibody signal specificity: correlations with the use of partial CB2-knockout mice and anti-rat CB2 receptor antibodies

    doi: 10.1038/s41401-018-0037-3

    Figure Lengend Snippet: mCB 2 immunostaining in midbrain DA neurons using the Alomone rCB 2 antibody. a Representative IHC staining results illustrating that the Alomone rCB 2 antibody detected significantly lower CB 2 immunostaining in the Zimmer strain of CB 2 -KO mice. b , c Diagrams showing the receptor-deleted regions and the binding site of the Alomone rCB 2 antibody in both strains of CB 2 -KO mice. d The mean densities of mCB 2 staining in DA neurons (over 200–400 DA neurons from 2 to 3 mice per genotype) illustrating a significant reduction in mCB 2 immunostaining in the Zimmer and Deltagen CB 2 -KO mice compared to the other strains. *** P

    Article Snippet: The epitopes of rat and mouse CB2 (mCB2 ) receptors differ by 5 amino acid residues. (2) The Alomone rCB2 polyclonal antibody was purchased from Alomone (ACR-002, Alomone Labs, Jerusalem, Israel), which recognizes the third intracellular loop.

    Techniques: Immunostaining, Immunohistochemistry, Staining, Mouse Assay, Binding Assay

    Western blot results in WT, CB 1 -KO, and Zimmer CB 2 -KO mice using four different CB 2 antibodies. a A diagram showing the binding sites of the four antibodies used in WB assays. b The Abcam rCB 2 -Ab detected an mCB 2 -like band at ~40 kD (mCB 2 monomer, 39 kD) in the striatum of all three mouse genotypes, but the other three antibodies (Alomone, NIH5633, Mackie) detected relatively lower densities of an mCB 2 -like band in Zimmer CB 2 -KO mice. c CB 2 -immunoreactive bands detected in the spleen using the same antibodies. More bands were detected in the striatum than the spleen by the multiple antibodies. Preabsorption of the CB 2 antibodies (Abcam, Alomone, NIH5633) with specific immune peptides blocked the immunoreactive bands

    Journal: Acta Pharmacologica Sinica

    Article Title: CB2 receptor antibody signal specificity: correlations with the use of partial CB2-knockout mice and anti-rat CB2 receptor antibodies

    doi: 10.1038/s41401-018-0037-3

    Figure Lengend Snippet: Western blot results in WT, CB 1 -KO, and Zimmer CB 2 -KO mice using four different CB 2 antibodies. a A diagram showing the binding sites of the four antibodies used in WB assays. b The Abcam rCB 2 -Ab detected an mCB 2 -like band at ~40 kD (mCB 2 monomer, 39 kD) in the striatum of all three mouse genotypes, but the other three antibodies (Alomone, NIH5633, Mackie) detected relatively lower densities of an mCB 2 -like band in Zimmer CB 2 -KO mice. c CB 2 -immunoreactive bands detected in the spleen using the same antibodies. More bands were detected in the striatum than the spleen by the multiple antibodies. Preabsorption of the CB 2 antibodies (Abcam, Alomone, NIH5633) with specific immune peptides blocked the immunoreactive bands

    Article Snippet: The epitopes of rat and mouse CB2 (mCB2 ) receptors differ by 5 amino acid residues. (2) The Alomone rCB2 polyclonal antibody was purchased from Alomone (ACR-002, Alomone Labs, Jerusalem, Israel), which recognizes the third intracellular loop.

    Techniques: Western Blot, Mouse Assay, Binding Assay

    Persistent inflammation does not change CB1 and CB2 mRNA levels but decreases CB1 protein levels. A , Relative quantities of mRNA for CB1 receptor expression in the RVM are not changed during persistent inflammation (unpaired t test, t (8) = 1.31). p =

    Journal: The Journal of Neuroscience

    Article Title: Compensatory Activation of Cannabinoid CB2 Receptor Inhibition of GABA Release in the Rostral Ventromedial Medulla in Inflammatory Pain

    doi: 10.1523/JNEUROSCI.1310-16.2016

    Figure Lengend Snippet: Persistent inflammation does not change CB1 and CB2 mRNA levels but decreases CB1 protein levels. A , Relative quantities of mRNA for CB1 receptor expression in the RVM are not changed during persistent inflammation (unpaired t test, t (8) = 1.31). p =

    Article Snippet: Blots were blocked with 5% milk (CB1) or 3% BSA (CB2) in PBS-T buffer (10.6 m m NaH2 PO4 , 56.5 m m Na2 HPO4 , 70 m m NaCl, 0.1% Tween 20, pH 7.4) at room temperature for 1 h. Blots were then incubated with rabbit CB1-L15 antibody (1:500; generous gift from Dr. Ken Mackie, Indiana University) or rabbit CB2 antibody (ACR-002, 1:200, Alomone Labs) overnight at 4°C.

    Techniques: Expressing

    inhibit GABA release in RVM neurons from CFA-treated rats. A , The CB2 agonist AM1241 (3 μ m ) significantly

    Journal: The Journal of Neuroscience

    Article Title: Compensatory Activation of Cannabinoid CB2 Receptor Inhibition of GABA Release in the Rostral Ventromedial Medulla in Inflammatory Pain

    doi: 10.1523/JNEUROSCI.1310-16.2016

    Figure Lengend Snippet: inhibit GABA release in RVM neurons from CFA-treated rats. A , The CB2 agonist AM1241 (3 μ m ) significantly

    Article Snippet: Blots were blocked with 5% milk (CB1) or 3% BSA (CB2) in PBS-T buffer (10.6 m m NaH2 PO4 , 56.5 m m Na2 HPO4 , 70 m m NaCl, 0.1% Tween 20, pH 7.4) at room temperature for 1 h. Blots were then incubated with rabbit CB1-L15 antibody (1:500; generous gift from Dr. Ken Mackie, Indiana University) or rabbit CB2 antibody (ACR-002, 1:200, Alomone Labs) overnight at 4°C.

    Techniques:

    Endocannabinoid tone is present in the RVM of CFA-treated rats. A , The CB2-selective antagonist SR144528 increases mIPSC frequency in CFA-treated rats but not naive rats (two-way repeated measure ANOVA, interaction, F (1,13) = 5.84, p = 0.03, Sidak's multiple

    Journal: The Journal of Neuroscience

    Article Title: Compensatory Activation of Cannabinoid CB2 Receptor Inhibition of GABA Release in the Rostral Ventromedial Medulla in Inflammatory Pain

    doi: 10.1523/JNEUROSCI.1310-16.2016

    Figure Lengend Snippet: Endocannabinoid tone is present in the RVM of CFA-treated rats. A , The CB2-selective antagonist SR144528 increases mIPSC frequency in CFA-treated rats but not naive rats (two-way repeated measure ANOVA, interaction, F (1,13) = 5.84, p = 0.03, Sidak's multiple

    Article Snippet: Blots were blocked with 5% milk (CB1) or 3% BSA (CB2) in PBS-T buffer (10.6 m m NaH2 PO4 , 56.5 m m Na2 HPO4 , 70 m m NaCl, 0.1% Tween 20, pH 7.4) at room temperature for 1 h. Blots were then incubated with rabbit CB1-L15 antibody (1:500; generous gift from Dr. Ken Mackie, Indiana University) or rabbit CB2 antibody (ACR-002, 1:200, Alomone Labs) overnight at 4°C.

    Techniques:

    The selective CB2 antagonist SR144528 blocks WIN55212 effects in CFA-treated but not naive rats. A , WIN55212 reduces mIPSC frequency in naive RVM neurons in the presence of the CB2 antagonist SR144528 (3 μ m ) (Wilcoxon matched-pairs signed rank

    Journal: The Journal of Neuroscience

    Article Title: Compensatory Activation of Cannabinoid CB2 Receptor Inhibition of GABA Release in the Rostral Ventromedial Medulla in Inflammatory Pain

    doi: 10.1523/JNEUROSCI.1310-16.2016

    Figure Lengend Snippet: The selective CB2 antagonist SR144528 blocks WIN55212 effects in CFA-treated but not naive rats. A , WIN55212 reduces mIPSC frequency in naive RVM neurons in the presence of the CB2 antagonist SR144528 (3 μ m ) (Wilcoxon matched-pairs signed rank

    Article Snippet: Blots were blocked with 5% milk (CB1) or 3% BSA (CB2) in PBS-T buffer (10.6 m m NaH2 PO4 , 56.5 m m Na2 HPO4 , 70 m m NaCl, 0.1% Tween 20, pH 7.4) at room temperature for 1 h. Blots were then incubated with rabbit CB1-L15 antibody (1:500; generous gift from Dr. Ken Mackie, Indiana University) or rabbit CB2 antibody (ACR-002, 1:200, Alomone Labs) overnight at 4°C.

    Techniques: