anti cannabinoid receptor 1 extracellular antibody (Alomone Labs)

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Alomone Labs anti cannabinoid receptor 1 extracellular antibody

Effect of <t>CB1-RNAi-LV</t> on activation and ECM production of primary hepatic stellate cells. ( A ) The statistical results of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) shows representative graphs of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I protein expression by Western blot analysis and ( C ) shows the statistical results. Results demonstrated that the mRNA and protein expressions of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I in HSCs were all increased significantly after 2-AG stimulation, which were all decreased significantly when stimulated with AM251 or transfected with CB1-RNAi-LV. Furthermore, the mRNA expression of α-SMA, TGF-β1, TGF-β RII and fibronectin in HSCs stimulated by 2-AG and AM251 was higher significantly than that in HSCs stimulated by 2-AG and transfected with CB1-RNAi-LV. “#” p

Anti Cannabinoid Receptor 1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cannabinoid receptor 1 extracellular antibody/product/Alomone Labs
Average 93 stars, based on 2 article reviews
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anti cannabinoid receptor 1 extracellular antibody - by Bioz Stars, 2022-12

93/100 stars

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1) Product Images from "Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats"

Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats

Journal: PLoS ONE

doi: 10.1371/journal.pone.0050850

Effect of CB1-RNAi-LV on activation and ECM production of primary hepatic stellate cells. ( A ) The statistical results of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) shows representative graphs of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I protein expression by Western blot analysis and ( C ) shows the statistical results. Results demonstrated that the mRNA and protein expressions of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I in HSCs were all increased significantly after 2-AG stimulation, which were all decreased significantly when stimulated with AM251 or transfected with CB1-RNAi-LV. Furthermore, the mRNA expression of α-SMA, TGF-β1, TGF-β RII and fibronectin in HSCs stimulated by 2-AG and AM251 was higher significantly than that in HSCs stimulated by 2-AG and transfected with CB1-RNAi-LV. “#” p

Figure Legend Snippet: Effect of CB1-RNAi-LV on activation and ECM production of primary hepatic stellate cells. ( A ) The statistical results of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) shows representative graphs of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I protein expression by Western blot analysis and ( C ) shows the statistical results. Results demonstrated that the mRNA and protein expressions of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I in HSCs were all increased significantly after 2-AG stimulation, which were all decreased significantly when stimulated with AM251 or transfected with CB1-RNAi-LV. Furthermore, the mRNA expression of α-SMA, TGF-β1, TGF-β RII and fibronectin in HSCs stimulated by 2-AG and AM251 was higher significantly than that in HSCs stimulated by 2-AG and transfected with CB1-RNAi-LV. “#” p

Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection

Effect of CB1-RNAi-LV on serum aminotransferase in DMN induced hepatic fibrosis. The statistical results of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in normal rats, rats with DMN treatment and Ringer's solution (RS) injected, rats with DMN treatment and NC-LV injected, rats with DMN treatment and CB1-RNAi-LV injected. “#” p

Figure Legend Snippet: Effect of CB1-RNAi-LV on serum aminotransferase in DMN induced hepatic fibrosis. The statistical results of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in normal rats, rats with DMN treatment and Ringer's solution (RS) injected, rats with DMN treatment and NC-LV injected, rats with DMN treatment and CB1-RNAi-LV injected. “#” p

Techniques Used: AST Assay, Injection

Effect of CB1-RNAi-LV on the proliferation of primary hepatic stellate cells. ( A ) The statistical results of the optical density (OD) value of BrdU incorporative cells in HSCs (named Con), HSCs with PDGF (named Con+PDGF), HSCs with NC-LV and PDGF (named NC+PDGF), HSCs with CB1-RNAi-LV and PDGF (named siRNA+PDGF) by ELISA method. “# #” p

Figure Legend Snippet: Effect of CB1-RNAi-LV on the proliferation of primary hepatic stellate cells. ( A ) The statistical results of the optical density (OD) value of BrdU incorporative cells in HSCs (named Con), HSCs with PDGF (named Con+PDGF), HSCs with NC-LV and PDGF (named NC+PDGF), HSCs with CB1-RNAi-LV and PDGF (named siRNA+PDGF) by ELISA method. “# #” p

Techniques Used: Enzyme-linked Immunosorbent Assay

Effect of shRNA expressing lentivirus against CB1 on CB1 expression in primary hepatic stellate cells. ( A ) The statistical results of CB1 mRNA expression in primary hepatic stellate cells (HSCs) transfected with 4 pairs of shRNA expressing lentivirus named KD1, KD2, KD3 and KD4 by RT-PCR detection. The pGCSIL/U6 mock vector named Con was used as a negative control. KD4 displayed the highest gene-silencing efficacy among all the 4 pairs of lentivirus. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) The CB1 protein expression in HSCs transfected with 4 pairs of shRNA expressing lentivirus and the negative control lentivirus, respectively. The results demonstrated that KD4 deduced CB1 mRNA expression by about 75%, and protein expression by about 80%, which had the highest gene-silencing efficacy among all the four pairs of lentivirus vectors.

Figure Legend Snippet: Effect of shRNA expressing lentivirus against CB1 on CB1 expression in primary hepatic stellate cells. ( A ) The statistical results of CB1 mRNA expression in primary hepatic stellate cells (HSCs) transfected with 4 pairs of shRNA expressing lentivirus named KD1, KD2, KD3 and KD4 by RT-PCR detection. The pGCSIL/U6 mock vector named Con was used as a negative control. KD4 displayed the highest gene-silencing efficacy among all the 4 pairs of lentivirus. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) The CB1 protein expression in HSCs transfected with 4 pairs of shRNA expressing lentivirus and the negative control lentivirus, respectively. The results demonstrated that KD4 deduced CB1 mRNA expression by about 75%, and protein expression by about 80%, which had the highest gene-silencing efficacy among all the four pairs of lentivirus vectors.

Techniques Used: shRNA, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Plasmid Preparation, Negative Control

Effect of CB1-RNAi-LV on hepatic fibrosis and epithelial- mesenchymal transitions in livers of DMN rats. ( A ) The first line showed the representative graph of Masson's trichrome staining (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). The second, the third and the fourth line showed the representative graph of CB1, α-SMA and E-cadherin immunohistochemical examination (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). ( B ) Shows statistical results of CB1, α-SMA and E-cadherin immunohistochemical staining. Masson's trichrome staining of liver sections in the DMN rats and the NC-LV treatment rats showed periportal fibrosis with short septa extending into lobules or porto-portal septa, and severe cholestasis and bile duct hyperplasia were also observed, whereas hepatic fibrosis was significantly ameliorated in the CB1-RNAi-LV treatment rats as compared with the NC-LV treatment rats. Immunohistochemical examination revealed that CB1 and α-SMA protein expressions increased significantly in the DMN rats, which were suppressed by CB1-RNAi-LV treatment. Immunochemical examination also showed that DMN treatment declined E-cadherin expression significantly compared with normal rats, while E-cadherin expression elevated significantly in DMN rats transfected with CB1-RNAi-LV, compared with that in DMN rats transfected with NC-LV. “# #” p

Figure Legend Snippet: Effect of CB1-RNAi-LV on hepatic fibrosis and epithelial- mesenchymal transitions in livers of DMN rats. ( A ) The first line showed the representative graph of Masson's trichrome staining (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). The second, the third and the fourth line showed the representative graph of CB1, α-SMA and E-cadherin immunohistochemical examination (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). ( B ) Shows statistical results of CB1, α-SMA and E-cadherin immunohistochemical staining. Masson's trichrome staining of liver sections in the DMN rats and the NC-LV treatment rats showed periportal fibrosis with short septa extending into lobules or porto-portal septa, and severe cholestasis and bile duct hyperplasia were also observed, whereas hepatic fibrosis was significantly ameliorated in the CB1-RNAi-LV treatment rats as compared with the NC-LV treatment rats. Immunohistochemical examination revealed that CB1 and α-SMA protein expressions increased significantly in the DMN rats, which were suppressed by CB1-RNAi-LV treatment. Immunochemical examination also showed that DMN treatment declined E-cadherin expression significantly compared with normal rats, while E-cadherin expression elevated significantly in DMN rats transfected with CB1-RNAi-LV, compared with that in DMN rats transfected with NC-LV. “# #” p

Techniques Used: Staining, Injection, Immunohistochemistry, Expressing, Transfection

Effect of CB1-RNAi-LV on Mesenchymal-Epithelial Transitions of primary hepatic stellate cells. ( A ) The statistical results of E-cadherin, Snail and Vimentin mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. “# #” p

Figure Legend Snippet: Effect of CB1-RNAi-LV on Mesenchymal-Epithelial Transitions of primary hepatic stellate cells. ( A ) The statistical results of E-cadherin, Snail and Vimentin mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. “# #” p

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction

2) Product Images from "Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks"

Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

doi: 10.1523/JNEUROSCI.1229-12.2012

Figure Legend Snippet: Increasing CB1 receptor activation reduces AMPA mPSC frequency

Techniques Used: Activation Assay

Figure Legend Snippet: Blocking CB1 receptors increased AMPA mPSC frequency

Techniques Used: Blocking Assay

Figure Legend Snippet: CB1 receptors are present in the developing chick spinal cord

Techniques Used:

Figure Legend Snippet: Chronic blockade of CB1 receptors increased embryonic limb movements in ovo

Techniques Used: In Ovo

Figure Legend Snippet: The CB1 agonist ACEA decreased AMPA mPSC frequency

Techniques Used:

Figure Legend Snippet: CB1 signaling does not influence evoked glutamatergic responses

Techniques Used:

Chronic blockade of CB1 receptors induce compensatory reductions of AMPA- and GABA mPSC amplitude in embryonic spinal motoneurons

Figure Legend Snippet: Chronic blockade of CB1 receptors induce compensatory reductions of AMPA- and GABA mPSC amplitude in embryonic spinal motoneurons

Techniques Used:

anti cannabinoid receptor 1 extracellular antibody (Alomone Labs)

Structured Review

Images

1) Product Images from "Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats"

2) Product Images from "Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks"

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