antibody against cb1 receptor  (Alomone Labs)


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    Name:
    Anti Cannabinoid Receptor 1 extracellular Antibody
    Description:
    Anti Cannabinoid Receptor 1 extracellular Antibody ACR 001 is a highly specific antibody directed against an epitope of the rat CB1 receptor The antibody can be used in western blot immunohistochemistry and live cell imaging applications It has been designed to recognize CB1 in human mouse and rat samples
    Catalog Number:
    ACR-001
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs antibody against cb1 receptor
    Anti Cannabinoid Receptor 1 extracellular Antibody
    Anti Cannabinoid Receptor 1 extracellular Antibody ACR 001 is a highly specific antibody directed against an epitope of the rat CB1 receptor The antibody can be used in western blot immunohistochemistry and live cell imaging applications It has been designed to recognize CB1 in human mouse and rat samples
    https://www.bioz.com/result/antibody against cb1 receptor/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against cb1 receptor - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks"

    Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    doi: 10.1523/JNEUROSCI.1229-12.2012

    Increasing CB1 receptor activation reduces AMPA mPSC frequency
    Figure Legend Snippet: Increasing CB1 receptor activation reduces AMPA mPSC frequency

    Techniques Used: Activation Assay

    Blocking CB1 receptors increased AMPA mPSC frequency
    Figure Legend Snippet: Blocking CB1 receptors increased AMPA mPSC frequency

    Techniques Used: Blocking Assay

    CB1 receptors are present in the developing chick spinal cord
    Figure Legend Snippet: CB1 receptors are present in the developing chick spinal cord

    Techniques Used:

    Chronic blockade of CB1 receptors increased embryonic limb movements in ovo
    Figure Legend Snippet: Chronic blockade of CB1 receptors increased embryonic limb movements in ovo

    Techniques Used: In Ovo

    The CB1 agonist ACEA decreased AMPA mPSC frequency
    Figure Legend Snippet: The CB1 agonist ACEA decreased AMPA mPSC frequency

    Techniques Used:

    CB1 signaling does not influence evoked glutamatergic responses
    Figure Legend Snippet: CB1 signaling does not influence evoked glutamatergic responses

    Techniques Used:

    Chronic blockade of CB1 receptors induce compensatory reductions of AMPA- and GABA mPSC amplitude in embryonic spinal motoneurons
    Figure Legend Snippet: Chronic blockade of CB1 receptors induce compensatory reductions of AMPA- and GABA mPSC amplitude in embryonic spinal motoneurons

    Techniques Used:

    2) Product Images from "Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats"

    Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050850

    Effect of CB1-RNAi-LV on activation and ECM production of primary hepatic stellate cells. ( A ) The statistical results of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) shows representative graphs of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I protein expression by Western blot analysis and ( C ) shows the statistical results. Results demonstrated that the mRNA and protein expressions of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I in HSCs were all increased significantly after 2-AG stimulation, which were all decreased significantly when stimulated with AM251 or transfected with CB1-RNAi-LV. Furthermore, the mRNA expression of α-SMA, TGF-β1, TGF-β RII and fibronectin in HSCs stimulated by 2-AG and AM251 was higher significantly than that in HSCs stimulated by 2-AG and transfected with CB1-RNAi-LV. “#” p
    Figure Legend Snippet: Effect of CB1-RNAi-LV on activation and ECM production of primary hepatic stellate cells. ( A ) The statistical results of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) shows representative graphs of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I protein expression by Western blot analysis and ( C ) shows the statistical results. Results demonstrated that the mRNA and protein expressions of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I in HSCs were all increased significantly after 2-AG stimulation, which were all decreased significantly when stimulated with AM251 or transfected with CB1-RNAi-LV. Furthermore, the mRNA expression of α-SMA, TGF-β1, TGF-β RII and fibronectin in HSCs stimulated by 2-AG and AM251 was higher significantly than that in HSCs stimulated by 2-AG and transfected with CB1-RNAi-LV. “#” p

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection

    Effect of CB1-RNAi-LV on serum aminotransferase in DMN induced hepatic fibrosis. The statistical results of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in normal rats, rats with DMN treatment and Ringer's solution (RS) injected, rats with DMN treatment and NC-LV injected, rats with DMN treatment and CB1-RNAi-LV injected. “#” p
    Figure Legend Snippet: Effect of CB1-RNAi-LV on serum aminotransferase in DMN induced hepatic fibrosis. The statistical results of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in normal rats, rats with DMN treatment and Ringer's solution (RS) injected, rats with DMN treatment and NC-LV injected, rats with DMN treatment and CB1-RNAi-LV injected. “#” p

    Techniques Used: AST Assay, Injection

    Effect of CB1-RNAi-LV on the proliferation of primary hepatic stellate cells. ( A ) The statistical results of the optical density (OD) value of BrdU incorporative cells in HSCs (named Con), HSCs with PDGF (named Con+PDGF), HSCs with NC-LV and PDGF (named NC+PDGF), HSCs with CB1-RNAi-LV and PDGF (named siRNA+PDGF) by ELISA method. “# #” p
    Figure Legend Snippet: Effect of CB1-RNAi-LV on the proliferation of primary hepatic stellate cells. ( A ) The statistical results of the optical density (OD) value of BrdU incorporative cells in HSCs (named Con), HSCs with PDGF (named Con+PDGF), HSCs with NC-LV and PDGF (named NC+PDGF), HSCs with CB1-RNAi-LV and PDGF (named siRNA+PDGF) by ELISA method. “# #” p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Effect of shRNA expressing lentivirus against CB1 on CB1 expression in primary hepatic stellate cells. ( A ) The statistical results of CB1 mRNA expression in primary hepatic stellate cells (HSCs) transfected with 4 pairs of shRNA expressing lentivirus named KD1, KD2, KD3 and KD4 by RT-PCR detection. The pGCSIL/U6 mock vector named Con was used as a negative control. KD4 displayed the highest gene-silencing efficacy among all the 4 pairs of lentivirus. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) The CB1 protein expression in HSCs transfected with 4 pairs of shRNA expressing lentivirus and the negative control lentivirus, respectively. The results demonstrated that KD4 deduced CB1 mRNA expression by about 75%, and protein expression by about 80%, which had the highest gene-silencing efficacy among all the four pairs of lentivirus vectors.
    Figure Legend Snippet: Effect of shRNA expressing lentivirus against CB1 on CB1 expression in primary hepatic stellate cells. ( A ) The statistical results of CB1 mRNA expression in primary hepatic stellate cells (HSCs) transfected with 4 pairs of shRNA expressing lentivirus named KD1, KD2, KD3 and KD4 by RT-PCR detection. The pGCSIL/U6 mock vector named Con was used as a negative control. KD4 displayed the highest gene-silencing efficacy among all the 4 pairs of lentivirus. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) The CB1 protein expression in HSCs transfected with 4 pairs of shRNA expressing lentivirus and the negative control lentivirus, respectively. The results demonstrated that KD4 deduced CB1 mRNA expression by about 75%, and protein expression by about 80%, which had the highest gene-silencing efficacy among all the four pairs of lentivirus vectors.

    Techniques Used: shRNA, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Plasmid Preparation, Negative Control

    Effect of CB1-RNAi-LV on hepatic fibrosis and epithelial- mesenchymal transitions in livers of DMN rats. ( A ) The first line showed the representative graph of Masson's trichrome staining (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). The second, the third and the fourth line showed the representative graph of CB1, α-SMA and E-cadherin immunohistochemical examination (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). ( B ) Shows statistical results of CB1, α-SMA and E-cadherin immunohistochemical staining. Masson's trichrome staining of liver sections in the DMN rats and the NC-LV treatment rats showed periportal fibrosis with short septa extending into lobules or porto-portal septa, and severe cholestasis and bile duct hyperplasia were also observed, whereas hepatic fibrosis was significantly ameliorated in the CB1-RNAi-LV treatment rats as compared with the NC-LV treatment rats. Immunohistochemical examination revealed that CB1 and α-SMA protein expressions increased significantly in the DMN rats, which were suppressed by CB1-RNAi-LV treatment. Immunochemical examination also showed that DMN treatment declined E-cadherin expression significantly compared with normal rats, while E-cadherin expression elevated significantly in DMN rats transfected with CB1-RNAi-LV, compared with that in DMN rats transfected with NC-LV. “# #” p
    Figure Legend Snippet: Effect of CB1-RNAi-LV on hepatic fibrosis and epithelial- mesenchymal transitions in livers of DMN rats. ( A ) The first line showed the representative graph of Masson's trichrome staining (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). The second, the third and the fourth line showed the representative graph of CB1, α-SMA and E-cadherin immunohistochemical examination (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). ( B ) Shows statistical results of CB1, α-SMA and E-cadherin immunohistochemical staining. Masson's trichrome staining of liver sections in the DMN rats and the NC-LV treatment rats showed periportal fibrosis with short septa extending into lobules or porto-portal septa, and severe cholestasis and bile duct hyperplasia were also observed, whereas hepatic fibrosis was significantly ameliorated in the CB1-RNAi-LV treatment rats as compared with the NC-LV treatment rats. Immunohistochemical examination revealed that CB1 and α-SMA protein expressions increased significantly in the DMN rats, which were suppressed by CB1-RNAi-LV treatment. Immunochemical examination also showed that DMN treatment declined E-cadherin expression significantly compared with normal rats, while E-cadherin expression elevated significantly in DMN rats transfected with CB1-RNAi-LV, compared with that in DMN rats transfected with NC-LV. “# #” p

    Techniques Used: Staining, Injection, Immunohistochemistry, Expressing, Transfection

    Effect of CB1-RNAi-LV on Mesenchymal-Epithelial Transitions of primary hepatic stellate cells. ( A ) The statistical results of E-cadherin, Snail and Vimentin mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. “# #” p
    Figure Legend Snippet: Effect of CB1-RNAi-LV on Mesenchymal-Epithelial Transitions of primary hepatic stellate cells. ( A ) The statistical results of E-cadherin, Snail and Vimentin mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. “# #” p

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction

    Related Articles

    Incubation:

    Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats
    Article Snippet: .. Immunoblotting was performed with primary antibodies against CB1 (Alomone labs, CA, USA), αSMA (Boster, Wuhan, China), TGF-β1 (CST, Beverly, USA), transforming growth factor β receptor II (TGF-β RII, CST, Beverly, USA), fibronectin (Santa Cruz, Santa Cruz, USA), procollagen Type I (Santa Cruz, Santa Cruz, USA), E-cadherin (Santa Cruz, Santa Cruz, USA), snail (CST, Beverly, USA), vimentin (CST, Beverly, USA) and GAPDH (Santa Cruz, Santa Cruz, USA), followed by incubation with horseradish peroxidase-conjugated anti-mouse- or anti-rabbit IgG (Santa Cruz, Santa Cruz, CA, USA) as secondary antibodies. ..

    Staining:

    Article Title: Dual inhibition of cannabinoid CB1 receptor and inducible NOS attenuates obesity‐induced chronic kidney disease, et al. Dual inhibition of cannabinoid CB1 receptors and inducible NOS attenuates obesity‐induced chronic kidney disease
    Article Snippet: .. Human kidney biopsies were stained with rabbit anti‐human CB1 receptor (1:100; Alomone, Cat# ACR‐001, RRID:AB_2039795) and iNOS (1:100; Abcam, Cat# ab3523, RRID:AB_303872), followed by an HRP One‐Step Polymer Detection System: anti‐Mouse–Rabbit–Rat ZyoChem. ..

    Article Title: SGIP1 alters internalization and modulates signaling of activated cannabinoid receptor 1 in a biased manner.
    Article Snippet: .. Homemade affinity purified polyclonal antibodies: guinea pig anti-SGIP1 1:500, rabbit anti-Bassoon (KK06/A63) 1:2000 (kind gift from Eckart D. Gundelfinger and Anna Fejtova from Leibniz Institute for Neurobiology, Germany) and commercial polyclonal rabbit anti-CB1R 1:200 (Alomone Labs, Israel), mouse monoclonal anti-MAP2 1:2000, mouse monoclonal anti-Tau1 (Millipore, Czech Republic) 1:500 and anti-Flag M2 antibody (Sigma Aldrich, Czech Republic) 1:500 were used for tandem staining of endogenous proteins as indicated. ..

    Affinity Purification:

    Article Title: SGIP1 alters internalization and modulates signaling of activated cannabinoid receptor 1 in a biased manner.
    Article Snippet: .. Homemade affinity purified polyclonal antibodies: guinea pig anti-SGIP1 1:500, rabbit anti-Bassoon (KK06/A63) 1:2000 (kind gift from Eckart D. Gundelfinger and Anna Fejtova from Leibniz Institute for Neurobiology, Germany) and commercial polyclonal rabbit anti-CB1R 1:200 (Alomone Labs, Israel), mouse monoclonal anti-MAP2 1:2000, mouse monoclonal anti-Tau1 (Millipore, Czech Republic) 1:500 and anti-Flag M2 antibody (Sigma Aldrich, Czech Republic) 1:500 were used for tandem staining of endogenous proteins as indicated. ..

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  • 92
    Alomone Labs antibody against cb1 receptor
    Increasing <t>CB1</t> receptor activation reduces AMPA mPSC frequency
    Antibody Against Cb1 Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against cb1 receptor/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against cb1 receptor - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Increasing CB1 receptor activation reduces AMPA mPSC frequency

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

    doi: 10.1523/JNEUROSCI.1229-12.2012

    Figure Lengend Snippet: Increasing CB1 receptor activation reduces AMPA mPSC frequency

    Article Snippet: The primary antibody against CB1 receptor was from Alomone Labs (rabbit anti-CB1R ACR-001).

    Techniques: Activation Assay

    Blocking CB1 receptors increased AMPA mPSC frequency

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

    doi: 10.1523/JNEUROSCI.1229-12.2012

    Figure Lengend Snippet: Blocking CB1 receptors increased AMPA mPSC frequency

    Article Snippet: The primary antibody against CB1 receptor was from Alomone Labs (rabbit anti-CB1R ACR-001).

    Techniques: Blocking Assay

    CB1 receptors are present in the developing chick spinal cord

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

    doi: 10.1523/JNEUROSCI.1229-12.2012

    Figure Lengend Snippet: CB1 receptors are present in the developing chick spinal cord

    Article Snippet: The primary antibody against CB1 receptor was from Alomone Labs (rabbit anti-CB1R ACR-001).

    Techniques:

    Chronic blockade of CB1 receptors increased embryonic limb movements in ovo

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

    doi: 10.1523/JNEUROSCI.1229-12.2012

    Figure Lengend Snippet: Chronic blockade of CB1 receptors increased embryonic limb movements in ovo

    Article Snippet: The primary antibody against CB1 receptor was from Alomone Labs (rabbit anti-CB1R ACR-001).

    Techniques: In Ovo

    The CB1 agonist ACEA decreased AMPA mPSC frequency

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

    doi: 10.1523/JNEUROSCI.1229-12.2012

    Figure Lengend Snippet: The CB1 agonist ACEA decreased AMPA mPSC frequency

    Article Snippet: The primary antibody against CB1 receptor was from Alomone Labs (rabbit anti-CB1R ACR-001).

    Techniques:

    CB1 signaling does not influence evoked glutamatergic responses

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

    doi: 10.1523/JNEUROSCI.1229-12.2012

    Figure Lengend Snippet: CB1 signaling does not influence evoked glutamatergic responses

    Article Snippet: The primary antibody against CB1 receptor was from Alomone Labs (rabbit anti-CB1R ACR-001).

    Techniques:

    Chronic blockade of CB1 receptors induce compensatory reductions of AMPA- and GABA mPSC amplitude in embryonic spinal motoneurons

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Tonic and transient endocannabinoid regulation of AMPAergic mPSCs and homeostatic plasticity in embryonic motor networks

    doi: 10.1523/JNEUROSCI.1229-12.2012

    Figure Lengend Snippet: Chronic blockade of CB1 receptors induce compensatory reductions of AMPA- and GABA mPSC amplitude in embryonic spinal motoneurons

    Article Snippet: The primary antibody against CB1 receptor was from Alomone Labs (rabbit anti-CB1R ACR-001).

    Techniques:

    Effect of CB1-RNAi-LV on activation and ECM production of primary hepatic stellate cells. ( A ) The statistical results of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) shows representative graphs of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I protein expression by Western blot analysis and ( C ) shows the statistical results. Results demonstrated that the mRNA and protein expressions of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I in HSCs were all increased significantly after 2-AG stimulation, which were all decreased significantly when stimulated with AM251 or transfected with CB1-RNAi-LV. Furthermore, the mRNA expression of α-SMA, TGF-β1, TGF-β RII and fibronectin in HSCs stimulated by 2-AG and AM251 was higher significantly than that in HSCs stimulated by 2-AG and transfected with CB1-RNAi-LV. “#” p

    Journal: PLoS ONE

    Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats

    doi: 10.1371/journal.pone.0050850

    Figure Lengend Snippet: Effect of CB1-RNAi-LV on activation and ECM production of primary hepatic stellate cells. ( A ) The statistical results of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) shows representative graphs of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I protein expression by Western blot analysis and ( C ) shows the statistical results. Results demonstrated that the mRNA and protein expressions of α-SMA, TGF-β1, TGF-β RII, fibronectin and procollagen type I in HSCs were all increased significantly after 2-AG stimulation, which were all decreased significantly when stimulated with AM251 or transfected with CB1-RNAi-LV. Furthermore, the mRNA expression of α-SMA, TGF-β1, TGF-β RII and fibronectin in HSCs stimulated by 2-AG and AM251 was higher significantly than that in HSCs stimulated by 2-AG and transfected with CB1-RNAi-LV. “#” p

    Article Snippet: Immunoblotting was performed with primary antibodies against CB1 (Alomone labs, CA, USA), αSMA (Boster, Wuhan, China), TGF-β1 (CST, Beverly, USA), transforming growth factor β receptor II (TGF-β RII, CST, Beverly, USA), fibronectin (Santa Cruz, Santa Cruz, USA), procollagen Type I (Santa Cruz, Santa Cruz, USA), E-cadherin (Santa Cruz, Santa Cruz, USA), snail (CST, Beverly, USA), vimentin (CST, Beverly, USA) and GAPDH (Santa Cruz, Santa Cruz, USA), followed by incubation with horseradish peroxidase-conjugated anti-mouse- or anti-rabbit IgG (Santa Cruz, Santa Cruz, CA, USA) as secondary antibodies.

    Techniques: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection

    Effect of CB1-RNAi-LV on serum aminotransferase in DMN induced hepatic fibrosis. The statistical results of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in normal rats, rats with DMN treatment and Ringer's solution (RS) injected, rats with DMN treatment and NC-LV injected, rats with DMN treatment and CB1-RNAi-LV injected. “#” p

    Journal: PLoS ONE

    Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats

    doi: 10.1371/journal.pone.0050850

    Figure Lengend Snippet: Effect of CB1-RNAi-LV on serum aminotransferase in DMN induced hepatic fibrosis. The statistical results of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in normal rats, rats with DMN treatment and Ringer's solution (RS) injected, rats with DMN treatment and NC-LV injected, rats with DMN treatment and CB1-RNAi-LV injected. “#” p

    Article Snippet: Immunoblotting was performed with primary antibodies against CB1 (Alomone labs, CA, USA), αSMA (Boster, Wuhan, China), TGF-β1 (CST, Beverly, USA), transforming growth factor β receptor II (TGF-β RII, CST, Beverly, USA), fibronectin (Santa Cruz, Santa Cruz, USA), procollagen Type I (Santa Cruz, Santa Cruz, USA), E-cadherin (Santa Cruz, Santa Cruz, USA), snail (CST, Beverly, USA), vimentin (CST, Beverly, USA) and GAPDH (Santa Cruz, Santa Cruz, USA), followed by incubation with horseradish peroxidase-conjugated anti-mouse- or anti-rabbit IgG (Santa Cruz, Santa Cruz, CA, USA) as secondary antibodies.

    Techniques: AST Assay, Injection

    Effect of CB1-RNAi-LV on the proliferation of primary hepatic stellate cells. ( A ) The statistical results of the optical density (OD) value of BrdU incorporative cells in HSCs (named Con), HSCs with PDGF (named Con+PDGF), HSCs with NC-LV and PDGF (named NC+PDGF), HSCs with CB1-RNAi-LV and PDGF (named siRNA+PDGF) by ELISA method. “# #” p

    Journal: PLoS ONE

    Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats

    doi: 10.1371/journal.pone.0050850

    Figure Lengend Snippet: Effect of CB1-RNAi-LV on the proliferation of primary hepatic stellate cells. ( A ) The statistical results of the optical density (OD) value of BrdU incorporative cells in HSCs (named Con), HSCs with PDGF (named Con+PDGF), HSCs with NC-LV and PDGF (named NC+PDGF), HSCs with CB1-RNAi-LV and PDGF (named siRNA+PDGF) by ELISA method. “# #” p

    Article Snippet: Immunoblotting was performed with primary antibodies against CB1 (Alomone labs, CA, USA), αSMA (Boster, Wuhan, China), TGF-β1 (CST, Beverly, USA), transforming growth factor β receptor II (TGF-β RII, CST, Beverly, USA), fibronectin (Santa Cruz, Santa Cruz, USA), procollagen Type I (Santa Cruz, Santa Cruz, USA), E-cadherin (Santa Cruz, Santa Cruz, USA), snail (CST, Beverly, USA), vimentin (CST, Beverly, USA) and GAPDH (Santa Cruz, Santa Cruz, USA), followed by incubation with horseradish peroxidase-conjugated anti-mouse- or anti-rabbit IgG (Santa Cruz, Santa Cruz, CA, USA) as secondary antibodies.

    Techniques: Enzyme-linked Immunosorbent Assay

    Effect of shRNA expressing lentivirus against CB1 on CB1 expression in primary hepatic stellate cells. ( A ) The statistical results of CB1 mRNA expression in primary hepatic stellate cells (HSCs) transfected with 4 pairs of shRNA expressing lentivirus named KD1, KD2, KD3 and KD4 by RT-PCR detection. The pGCSIL/U6 mock vector named Con was used as a negative control. KD4 displayed the highest gene-silencing efficacy among all the 4 pairs of lentivirus. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) The CB1 protein expression in HSCs transfected with 4 pairs of shRNA expressing lentivirus and the negative control lentivirus, respectively. The results demonstrated that KD4 deduced CB1 mRNA expression by about 75%, and protein expression by about 80%, which had the highest gene-silencing efficacy among all the four pairs of lentivirus vectors.

    Journal: PLoS ONE

    Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats

    doi: 10.1371/journal.pone.0050850

    Figure Lengend Snippet: Effect of shRNA expressing lentivirus against CB1 on CB1 expression in primary hepatic stellate cells. ( A ) The statistical results of CB1 mRNA expression in primary hepatic stellate cells (HSCs) transfected with 4 pairs of shRNA expressing lentivirus named KD1, KD2, KD3 and KD4 by RT-PCR detection. The pGCSIL/U6 mock vector named Con was used as a negative control. KD4 displayed the highest gene-silencing efficacy among all the 4 pairs of lentivirus. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. ( B ) The CB1 protein expression in HSCs transfected with 4 pairs of shRNA expressing lentivirus and the negative control lentivirus, respectively. The results demonstrated that KD4 deduced CB1 mRNA expression by about 75%, and protein expression by about 80%, which had the highest gene-silencing efficacy among all the four pairs of lentivirus vectors.

    Article Snippet: Immunoblotting was performed with primary antibodies against CB1 (Alomone labs, CA, USA), αSMA (Boster, Wuhan, China), TGF-β1 (CST, Beverly, USA), transforming growth factor β receptor II (TGF-β RII, CST, Beverly, USA), fibronectin (Santa Cruz, Santa Cruz, USA), procollagen Type I (Santa Cruz, Santa Cruz, USA), E-cadherin (Santa Cruz, Santa Cruz, USA), snail (CST, Beverly, USA), vimentin (CST, Beverly, USA) and GAPDH (Santa Cruz, Santa Cruz, USA), followed by incubation with horseradish peroxidase-conjugated anti-mouse- or anti-rabbit IgG (Santa Cruz, Santa Cruz, CA, USA) as secondary antibodies.

    Techniques: shRNA, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Plasmid Preparation, Negative Control

    Effect of CB1-RNAi-LV on hepatic fibrosis and epithelial- mesenchymal transitions in livers of DMN rats. ( A ) The first line showed the representative graph of Masson's trichrome staining (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). The second, the third and the fourth line showed the representative graph of CB1, α-SMA and E-cadherin immunohistochemical examination (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). ( B ) Shows statistical results of CB1, α-SMA and E-cadherin immunohistochemical staining. Masson's trichrome staining of liver sections in the DMN rats and the NC-LV treatment rats showed periportal fibrosis with short septa extending into lobules or porto-portal septa, and severe cholestasis and bile duct hyperplasia were also observed, whereas hepatic fibrosis was significantly ameliorated in the CB1-RNAi-LV treatment rats as compared with the NC-LV treatment rats. Immunohistochemical examination revealed that CB1 and α-SMA protein expressions increased significantly in the DMN rats, which were suppressed by CB1-RNAi-LV treatment. Immunochemical examination also showed that DMN treatment declined E-cadherin expression significantly compared with normal rats, while E-cadherin expression elevated significantly in DMN rats transfected with CB1-RNAi-LV, compared with that in DMN rats transfected with NC-LV. “# #” p

    Journal: PLoS ONE

    Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats

    doi: 10.1371/journal.pone.0050850

    Figure Lengend Snippet: Effect of CB1-RNAi-LV on hepatic fibrosis and epithelial- mesenchymal transitions in livers of DMN rats. ( A ) The first line showed the representative graph of Masson's trichrome staining (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). The second, the third and the fourth line showed the representative graph of CB1, α-SMA and E-cadherin immunohistochemical examination (magnification of 20×) in normal rats (Normal), rats with DMN treatment and Ringer's solution injected (DMN+RS), rats with DMN treatment and NC-LV injected (DMN+NC-LV), rats with DMN treatment and CB1-RNAi-LV injected (DMN+RNAi-LV). ( B ) Shows statistical results of CB1, α-SMA and E-cadherin immunohistochemical staining. Masson's trichrome staining of liver sections in the DMN rats and the NC-LV treatment rats showed periportal fibrosis with short septa extending into lobules or porto-portal septa, and severe cholestasis and bile duct hyperplasia were also observed, whereas hepatic fibrosis was significantly ameliorated in the CB1-RNAi-LV treatment rats as compared with the NC-LV treatment rats. Immunohistochemical examination revealed that CB1 and α-SMA protein expressions increased significantly in the DMN rats, which were suppressed by CB1-RNAi-LV treatment. Immunochemical examination also showed that DMN treatment declined E-cadherin expression significantly compared with normal rats, while E-cadherin expression elevated significantly in DMN rats transfected with CB1-RNAi-LV, compared with that in DMN rats transfected with NC-LV. “# #” p

    Article Snippet: Immunoblotting was performed with primary antibodies against CB1 (Alomone labs, CA, USA), αSMA (Boster, Wuhan, China), TGF-β1 (CST, Beverly, USA), transforming growth factor β receptor II (TGF-β RII, CST, Beverly, USA), fibronectin (Santa Cruz, Santa Cruz, USA), procollagen Type I (Santa Cruz, Santa Cruz, USA), E-cadherin (Santa Cruz, Santa Cruz, USA), snail (CST, Beverly, USA), vimentin (CST, Beverly, USA) and GAPDH (Santa Cruz, Santa Cruz, USA), followed by incubation with horseradish peroxidase-conjugated anti-mouse- or anti-rabbit IgG (Santa Cruz, Santa Cruz, CA, USA) as secondary antibodies.

    Techniques: Staining, Injection, Immunohistochemistry, Expressing, Transfection

    Effect of CB1-RNAi-LV on Mesenchymal-Epithelial Transitions of primary hepatic stellate cells. ( A ) The statistical results of E-cadherin, Snail and Vimentin mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. “# #” p

    Journal: PLoS ONE

    Article Title: Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats

    doi: 10.1371/journal.pone.0050850

    Figure Lengend Snippet: Effect of CB1-RNAi-LV on Mesenchymal-Epithelial Transitions of primary hepatic stellate cells. ( A ) The statistical results of E-cadherin, Snail and Vimentin mRNA expression in HSCs (named Con), HSCs with 2-AG (named Con+2AG), HSCs with NC-LV and 2-AG (named NC+2AG), HSCs with CB1-RNAi-LV and 2-AG (named siRNA+2AG), HSCs with 2-AG and AM251 (named Con+2AG+AM251) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. “# #” p

    Article Snippet: Immunoblotting was performed with primary antibodies against CB1 (Alomone labs, CA, USA), αSMA (Boster, Wuhan, China), TGF-β1 (CST, Beverly, USA), transforming growth factor β receptor II (TGF-β RII, CST, Beverly, USA), fibronectin (Santa Cruz, Santa Cruz, USA), procollagen Type I (Santa Cruz, Santa Cruz, USA), E-cadherin (Santa Cruz, Santa Cruz, USA), snail (CST, Beverly, USA), vimentin (CST, Beverly, USA) and GAPDH (Santa Cruz, Santa Cruz, USA), followed by incubation with horseradish peroxidase-conjugated anti-mouse- or anti-rabbit IgG (Santa Cruz, Santa Cruz, CA, USA) as secondary antibodies.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction