anti pmca1 antibody  (Alomone Labs)


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    Alomone Labs anti pmca1 antibody
    Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using <t>anti-PMCA1</t> and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale in (A) = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Images in (A), (B), and (C) taken from the mid-apical region of the cochlea. Scale in (B) and (C) = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. Mouse 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. Mouse 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for Neuroplastin also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent Neuroplastin was correlated with a weak PMCA signal (*).
    Anti Pmca1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pmca1 antibody/product/Alomone Labs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti pmca1 antibody - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing"

    Article Title: Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1009937

    Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using anti-PMCA1 and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale in (A) = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Images in (A), (B), and (C) taken from the mid-apical region of the cochlea. Scale in (B) and (C) = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. Mouse 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. Mouse 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for Neuroplastin also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent Neuroplastin was correlated with a weak PMCA signal (*).
    Figure Legend Snippet: Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using anti-PMCA1 and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale in (A) = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Images in (A), (B), and (C) taken from the mid-apical region of the cochlea. Scale in (B) and (C) = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. Mouse 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. Mouse 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for Neuroplastin also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent Neuroplastin was correlated with a weak PMCA signal (*).

    Techniques Used: Mouse Assay, Staining

    2) Product Images from "Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing"

    Article Title: Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing

    Journal: bioRxiv

    doi: 10.1101/2021.11.10.468016

    Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using anti-PMCA1 and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Scale = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. individual 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. individual 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for NEUROPLASTIN also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent NEUROPLASTIN was correlated with a weak PMCA signal (*).
    Figure Legend Snippet: Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using anti-PMCA1 and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Scale = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. individual 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. individual 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for NEUROPLASTIN also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent NEUROPLASTIN was correlated with a weak PMCA signal (*).

    Techniques Used: Mouse Assay, Staining

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    Alomone Labs anti pmca1 antibody
    Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using <t>anti-PMCA1</t> and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale in (A) = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Images in (A), (B), and (C) taken from the mid-apical region of the cochlea. Scale in (B) and (C) = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. Mouse 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. Mouse 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for Neuroplastin also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent Neuroplastin was correlated with a weak PMCA signal (*).
    Anti Pmca1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pmca1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pmca1 antibody - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using anti-PMCA1 and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale in (A) = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Images in (A), (B), and (C) taken from the mid-apical region of the cochlea. Scale in (B) and (C) = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. Mouse 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. Mouse 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for Neuroplastin also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent Neuroplastin was correlated with a weak PMCA signal (*).

    Journal: PLoS Genetics

    Article Title: Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing

    doi: 10.1371/journal.pgen.1009937

    Figure Lengend Snippet: Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using anti-PMCA1 and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale in (A) = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Images in (A), (B), and (C) taken from the mid-apical region of the cochlea. Scale in (B) and (C) = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. Mouse 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. Mouse 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for Neuroplastin also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent Neuroplastin was correlated with a weak PMCA signal (*).

    Article Snippet: Primary antibodies: sheep anti-Np (1:200, R and D Systems Cat# AF7818, RRID:AB_2715517), goat anti-Np65 (1:100, R and D Systems Cat# AF5360, RRID:AB_2155920), mouse anti-PMCA 5F10 (1:200, Thermo Fisher Scientific Cat# MA3-914, RRID:AB_2061566), rabbit anti-PMCA1 (1:200, Alomone Labs Cat# ACP-005, RRID:AB_2756567), rabbit anti-PMCA2 (1:200, Abcam Cat# ab3529, RRID:AB_303878), rabbit anti-Neurofilament 200 (1:200, Sigma-Aldrich Cat# N4142, RRID:AB_477272).

    Techniques: Mouse Assay, Staining

    Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using anti-PMCA1 and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Scale = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. individual 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. individual 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for NEUROPLASTIN also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent NEUROPLASTIN was correlated with a weak PMCA signal (*).

    Journal: bioRxiv

    Article Title: Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing

    doi: 10.1101/2021.11.10.468016

    Figure Lengend Snippet: Hearing impairment is inversely correlated with Nptn-dependent localisation of PMCA in outer hair cell stereocilia. (A) Immunolabelling of cochlear cryosections using anti-PMCA1 and anti-PMCA2 antibodies. In wild type tissue, PMCA1 is predominantly localised to the membrane of IHCs and OHCs, whereas PMCA2 is localised to OHC stereocilia and lateral supporting cells. Labelling of the PMCAs at these locations was substantially reduced in Nptn tm1b/tm1b cochleae. Scale = 20 μm. (B) The anti-pan-PMCA antibody detects PMCA1-4. Maximum intensity projections of whole-mount cochlear immunolabelling using the anti-pan-PMCA antibody showed labelling of OHC stereocilia and membrane of IHCs in Nptn +/+ mice, which were substantially reduced, but not absent, in Nptn tm1b/tm1b cochleae. (C) Using the anti-pan-PMCA antibody, no labelling differences were seen in Nptn Δexon2/Δexon2 cochleae compared to wild-type. Scale = 10 μm. (D) ABR threshold and DPOAE response measures from two Nptn fl/fl ;Prestin-CreER T2 + mice following tamoxifen-induced cre-mediated recombination, with corresponding anti-pan-Np and anti-pan-PMCA immunolabelled mid-apical cochlear whole mounts (maximum intensity projections). Data from the individual mice are shown (red triangles) compared against wild type controls (black circles, mean ± SD). Due to varying levels of induction, the auditory phenotype exhibited was variable with some mice showing no significant phenotype (e.g. individual 1), while others showed increased ABR thresholds and a decreased DPOAE response (e.g. individual 2). The auditory phenotype was directly correlated to the level of Nptn recombination as demonstrated by immunolabelling using the anti-pan-Np antibody. Bundles that were strongly stained for NEUROPLASTIN also had strong labelling for anti-pan-PMCA antibody (arrowhead), whereas absent NEUROPLASTIN was correlated with a weak PMCA signal (*).

    Article Snippet: Primary antibodies: sheep anti-Np (1:200, R and D Systems Cat# AF7818, RRID:AB_2715517), goat anti-Np65 (1:100, R and D Systems Cat# AF5360, RRID:AB_2155920), mouse anti-PMCA 5F10 (1:200, Thermo Fisher Scientific Cat# MA3-914, RRID:AB_2061566), rabbit anti-PMCA1 (1:200, Alomone Labs Cat# ACP-005, RRID:AB_2756567), rabbit anti-PMCA2 (1:200, Abcam Cat# ab3529, RRID:AB_303878), rabbit anti-Neurofilament 200 (1:200, Sigma-Aldrich Cat# N4142, RRID:AB_477272).

    Techniques: Mouse Assay, Staining