Cftr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 94 stars, based on 1 article reviews
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1) Product Images from "CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury"
Article Title: CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury
Journal: Scientific Reports
Figure Legend Snippet: CFTR mediates heat-induced activation of MAPK/NF-κB/COX-2/PGE 2 in 16HBE14o- cells. ( a ) QRT-PCR analysis of CFTR and COX-2 in cells transfected with CFTR-silencing ribosome vectors (rib) or empty pEF6/V5-His vectors as negative control (pEF). ***P
Techniques Used: Activation Assay, Quantitative RT-PCR, Transfection, Negative Control
Figure Legend Snippet: IL-8 production as a result of MAPK/NF-κB/COX-2/PGE 2 activation by heat or CFTR knockdown in 16HBE14o- cells. ( a,b ) QRT-PCR analysis and ELISA detection of IL-8 production in cells with (+) or without (−) heat-treatment ( a ), transfection of pEF/rib ( b ) in the presence (+) or absence (−) of BAY11 (40 μM), PD98059 (20 μM), SP600125 (40 μM) or DMSO as vehicle control. ### P
Techniques Used: Activation Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Transfection
Figure Legend Snippet: Curcumin mitigates heat-induced inflammation by upregulating CFTR in airway epithelial cells in vitro and in vivo . ( a,b ) Western blotting for CFTR and COX-2 ( a ), and ELISA detection of PGE 2 and IL-8 ( b ) in 16HBE14o- cells before (−) and after (+) heat-treatment in the presence (+) or absence (−) of curcumin (Cur, 10 μM)or DMSO as vehicle control. Curcumin was administrated either 4 hours before (Pre), 0.5 hour after (Post, 0.5 h), or 8 hours after (Post, 8 h) the heat treatment. Tubulin was used as loading control. *P
Techniques Used: In Vitro, In Vivo, Western Blot, Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: Heat-induced temporal changes in CFTR and COX-2 expression in airway epithelial cells in vitro and in vivo . ( a,b ) 16HBE14o- cells were cultured at 37 °C till 80% confluence before incubated at 52 °C for 5 min as heat-treatment and subsequently back at 37 °C for recovery. Cells were collected before (Ctrl), after heat-treatment (0 h), or after recovery for 8 hours (8 h) to 5 days (5d) for QRT-PCR ( a ) and western blot ( b ) analysis of CFTR and COX-2. Data are means ± SEM from at least three independent experiments. ***P
Techniques Used: Expressing, In Vitro, In Vivo, Cell Culture, Incubation, Quantitative RT-PCR, Western Blot
2) Product Images from "Cystic Fibrosis Transmembrane Conductance Regulator: A Possible New Target for Photodynamic Therapy Enhances Wound Healing"
Article Title: Cystic Fibrosis Transmembrane Conductance Regulator: A Possible New Target for Photodynamic Therapy Enhances Wound Healing
Journal: Advances in Wound Care
Figure Legend Snippet: Expression of CFTR, phospho-FAK Tyr861, and phospho-paxillin in HaCaT cells after treatment with ICG-PDT-conditioned medium. (A) Immunofluorescence images of HaCaT cells treated with ICG-PDT-conditioned medium (5 J/cm 2 and 100 μg/mL ICG) at different time points. The cells were fixed and stained with the primary antibodies anti-CFTR ( green ) and phospho-FAK Tyr861 (p-FAK; red ), and DAPI ( blue ). The figures show representative images from three separated experiments. (B) Representative immunoblots from three separate experiments revealed dynamic expressions of CFTR, phospho-FAK Tyr861 (p-FAK), total FAK (t-FAK), phospho-paxillin Tyr 118 (p-paxillin), and β-actin in HaCaT cells treated with the same conditioned medium. (C) The protein expression levels were quantified with ImageJ software (* p
Techniques Used: Expressing, Immunofluorescence, Staining, Western Blot, Software
Figure Legend Snippet: Colocalization of CFTR and phosphorylated FAK Tyr861. (A) Representative confocal images from HaCaT cells expressing CFTR ( green ) in two connected cells from three separated experiments. (B) Phospho-FAK Tyr861 (p-FAK Tyr861; red ) and (C) merge were examined 6 h after incubation with ICG-PDT-treated (5 J/cm 2 and 100 μg/mL ICG) conditioned medium. (D) Line scan of fluorescence intensity (arbitrary unit, a.u.) showing high colocalization of CFTR and phospho-FAK Tyr861. Scale bar = 10 μm. CFTR, cystic fibrosis transmembrane conductance regulator; FAK, focal adhesion kinase.
Techniques Used: Expressing, Incubation, Fluorescence