anti megalin antibody  (Alomone Labs)


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    Alomone Labs anti megalin antibody
    Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, <t>anti-Megalin</t> antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).
    Anti Megalin Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti megalin antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti megalin antibody - by Bioz Stars, 2022-05
    86/100 stars

    Images

    1) Product Images from "Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells"

    Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064843

    Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).
    Figure Legend Snippet: Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).

    Techniques Used: Immunohistochemistry, Immunocytochemistry, Staining

    Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.
    Figure Legend Snippet: Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.

    Techniques Used: Polymerase Chain Reaction, Expressing, Purification, Flow Cytometry, Cytometry, Cell Culture, FACS, Immunofluorescence

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    Alomone Labs anti megalin antibody
    Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, <t>anti-Megalin</t> antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).
    Anti Megalin Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti megalin antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti megalin antibody - by Bioz Stars, 2022-05
    86/100 stars
      Buy from Supplier

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    Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).

    Journal: PLoS ONE

    Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells

    doi: 10.1371/journal.pone.0064843

    Figure Lengend Snippet: Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).

    Article Snippet: Immunofluorescent staining of mouse neonatal kidney tissue was then performed using the original anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody.

    Techniques: Immunohistochemistry, Immunocytochemistry, Staining

    Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.

    Journal: PLoS ONE

    Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells

    doi: 10.1371/journal.pone.0064843

    Figure Lengend Snippet: Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.

    Article Snippet: Immunofluorescent staining of mouse neonatal kidney tissue was then performed using the original anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody.

    Techniques: Polymerase Chain Reaction, Expressing, Purification, Flow Cytometry, Cytometry, Cell Culture, FACS, Immunofluorescence