anti megalin antibody  (Alomone Labs)


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    Name:
    Anti Aquaporin 2 Antibody
    Description:
    Anti Aquaporin 2 Antibody AQP 002 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize the AQP2 channel from rat mouse and human samples
    Catalog Number:
    AQP-002
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs anti megalin antibody
    Anti Aquaporin 2 Antibody
    Anti Aquaporin 2 Antibody AQP 002 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize the AQP2 channel from rat mouse and human samples
    https://www.bioz.com/result/anti megalin antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti megalin antibody - by Bioz Stars, 2021-09
    95/100 stars

    Images

    1) Product Images from "Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells"

    Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064843

    Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).
    Figure Legend Snippet: Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).

    Techniques Used: Immunohistochemistry, Immunocytochemistry, Staining

    Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.
    Figure Legend Snippet: Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.

    Techniques Used: Polymerase Chain Reaction, Expressing, Purification, Flow Cytometry, Cytometry, Cell Culture, FACS, Immunofluorescence

    Related Articles

    Incubation:

    Article Title: Angiotensin II increases activity of the ClC-K2 Cl− channel in collecting duct intercalated cells by stimulating production of reactive oxygen species
    Article Snippet: .. The samples were incubated overnight at +4 °C with anti-Aquaporin 2 antibody (1:4000 dilution; Alomone Labs, Cat. # AQP-002). ..

    Article Title: Deficiency of peroxisomal L-bifunctional protein (EHHADH) causes male-specific kidney hypertrophy and proximal tubular injury in mice
    Article Snippet: .. The incubation with primary antibody was performed in 0.1% Triton X-100 in 3% donkey normal serum in PBS, overnight at 4°C, using the following antibodies: anti-KIM-1 (AF1817, RD Systems), anti-SOX-9 (NBP1-8551, Novus Bio), anti-LRP2 (ab76969, Abcam), anti-SGLT1 (ab14685, Abcam), biotinylated-LTL (B-1325, Vector Laboratories), anti-EHHADH (GTX81126, Genetex), anti-Ki67 (MA5-14520, Invitrogen), anti-AQP2 (AQP-002, Alomone labs), anti-CALB (PA5-85669, Thermo Fisher), and anti-Slc34a3 (NPT2c, 20603, BiCell Scientific). ..

    Article Title: Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury
    Article Snippet: .. After blocking sections with PBS containing 10% normal rabbit serum, sections were incubated with 1:100 anti-PEG antibody (ab94764, Abcam) specific to the PEG backbone plus PHA lectin antibody (proximal tubule–specific marker, Molecular Probes) or plus aquaporin-2 antibody (collecting duct–specific marker, AQP-002, Alomone Labs). ..

    Article Title: Angiotensin II increases activity of the ClC-K2 Cl− channel in collecting duct intercalated cells by stimulating production of reactive oxygen species
    Article Snippet: .. For experiments with double staining, the samples were incubated with anti-Aquaporin 2 antibody (1:4000 dilution; Alomone Labs, Cat # AQP-002) overnight at 4 °C. ..

    Article Title: Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise
    Article Snippet: .. After blocking with a blocking solution (AQPs, 5% skimmed milk; caspase‐3, Blocking One [Nacalai Tesque]), the membranes were incubated overnight with the rabbit primary antibody anti‐AQP2 (1:5,000 dilution) (Alomone Labs, Jerusalem, Israel), anti‐AQP3 (1:10,000 dilution) (Alomone Labs), anti‐AQP4 (1:10,000 dilution) (Alomone Labs), or anti‐caspase‐3 (1:2000) (Cell Signaling Technology, Beverly, MA, USA) at 4°C with gentle shaking. ..

    Blocking Assay:

    Article Title: Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury
    Article Snippet: .. After blocking sections with PBS containing 10% normal rabbit serum, sections were incubated with 1:100 anti-PEG antibody (ab94764, Abcam) specific to the PEG backbone plus PHA lectin antibody (proximal tubule–specific marker, Molecular Probes) or plus aquaporin-2 antibody (collecting duct–specific marker, AQP-002, Alomone Labs). ..

    Article Title: Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise
    Article Snippet: .. After blocking with a blocking solution (AQPs, 5% skimmed milk; caspase‐3, Blocking One [Nacalai Tesque]), the membranes were incubated overnight with the rabbit primary antibody anti‐AQP2 (1:5,000 dilution) (Alomone Labs, Jerusalem, Israel), anti‐AQP3 (1:10,000 dilution) (Alomone Labs), anti‐AQP4 (1:10,000 dilution) (Alomone Labs), or anti‐caspase‐3 (1:2000) (Cell Signaling Technology, Beverly, MA, USA) at 4°C with gentle shaking. ..

    Marker:

    Article Title: Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury
    Article Snippet: .. After blocking sections with PBS containing 10% normal rabbit serum, sections were incubated with 1:100 anti-PEG antibody (ab94764, Abcam) specific to the PEG backbone plus PHA lectin antibody (proximal tubule–specific marker, Molecular Probes) or plus aquaporin-2 antibody (collecting duct–specific marker, AQP-002, Alomone Labs). ..

    Double Staining:

    Article Title: Angiotensin II increases activity of the ClC-K2 Cl− channel in collecting duct intercalated cells by stimulating production of reactive oxygen species
    Article Snippet: .. For experiments with double staining, the samples were incubated with anti-Aquaporin 2 antibody (1:4000 dilution; Alomone Labs, Cat # AQP-002) overnight at 4 °C. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Alomone Labs anti megalin antibody
    Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, <t>anti-Megalin</t> antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).
    Anti Megalin Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti megalin antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti megalin antibody - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).

    Journal: PLoS ONE

    Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells

    doi: 10.1371/journal.pone.0064843

    Figure Lengend Snippet: Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).

    Article Snippet: Immunofluorescent staining of mouse neonatal kidney tissue was then performed using the original anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody.

    Techniques: Immunohistochemistry, Immunocytochemistry, Staining

    Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.

    Journal: PLoS ONE

    Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells

    doi: 10.1371/journal.pone.0064843

    Figure Lengend Snippet: Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.

    Article Snippet: Immunofluorescent staining of mouse neonatal kidney tissue was then performed using the original anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody.

    Techniques: Polymerase Chain Reaction, Expressing, Purification, Flow Cytometry, Cytometry, Cell Culture, FACS, Immunofluorescence