Anti Megalin Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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1) Product Images from "Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells"
Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells
Journal: PLoS ONE
Figure Legend Snippet: Immunohistochemistry and immunocytochemistry was performed using our original anti-KSP antibody. (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).
Techniques Used: Immunohistochemistry, Immunocytochemistry, Staining
Figure Legend Snippet: Differentiation of KSP-positive cells into each segment of renal tubular cells. (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.
Techniques Used: Polymerase Chain Reaction, Expressing, Purification, Flow Cytometry, Cytometry, Cell Culture, FACS, Immunofluorescence