bdnf  (Alomone Labs)


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    Alomone Labs bdnf
    Up-regulation of <t>BDNF-induced</t> astroglial protein synthesis depends on the presence of neurons. (A) Schematic drawing of the lentiviral expression vector LVGFAPEGFP that expresses EGFP cell type-specifically in astrocytes under control of the simplified GFAP promoter version gfaABC 1 D. (B) Astrocytes expressed EGFP cell type-specifically after infection with LVGFAPEGFP in a neuron-glia coculture (DIV 22). Cultures were treated for 4 h with either 50 ng/ml BDNF or 2 μM <t>TTX</t> in the presence of 4 mM AHA and processed for 'click reaction' with a TAMRA-alkyne tag in the FUNCAT procedure. BDNF application led to an upregulation of TAMRA signal intensities up to 150%. TAMRA intensities are color coded in the lower panel (scale bar = 10 μm). (C) GFAP-positive astrocytes in monoculture (DIV 20–22) expressing EGFP show fibroblast-like morphology. No differences in TAMRA signal intensities were observed when cultures were treated analogous to (B) (scale bar = 10 μm). (D) Cultures were manipulated as described in (B). Mean TAMRA signal intensities were determined within the EGFP mask. A significant increase in TAMRA signal intensities was detected after BDNF treatment. Numbers at the X-axis indicate the number of cells included in the quantification (3 independent experiments; represented data are mean +/- SEM; ONE-way ANOVA, ***: p
    Bdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling"

    Article Title: Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0145451

    Up-regulation of BDNF-induced astroglial protein synthesis depends on the presence of neurons. (A) Schematic drawing of the lentiviral expression vector LVGFAPEGFP that expresses EGFP cell type-specifically in astrocytes under control of the simplified GFAP promoter version gfaABC 1 D. (B) Astrocytes expressed EGFP cell type-specifically after infection with LVGFAPEGFP in a neuron-glia coculture (DIV 22). Cultures were treated for 4 h with either 50 ng/ml BDNF or 2 μM TTX in the presence of 4 mM AHA and processed for 'click reaction' with a TAMRA-alkyne tag in the FUNCAT procedure. BDNF application led to an upregulation of TAMRA signal intensities up to 150%. TAMRA intensities are color coded in the lower panel (scale bar = 10 μm). (C) GFAP-positive astrocytes in monoculture (DIV 20–22) expressing EGFP show fibroblast-like morphology. No differences in TAMRA signal intensities were observed when cultures were treated analogous to (B) (scale bar = 10 μm). (D) Cultures were manipulated as described in (B). Mean TAMRA signal intensities were determined within the EGFP mask. A significant increase in TAMRA signal intensities was detected after BDNF treatment. Numbers at the X-axis indicate the number of cells included in the quantification (3 independent experiments; represented data are mean +/- SEM; ONE-way ANOVA, ***: p
    Figure Legend Snippet: Up-regulation of BDNF-induced astroglial protein synthesis depends on the presence of neurons. (A) Schematic drawing of the lentiviral expression vector LVGFAPEGFP that expresses EGFP cell type-specifically in astrocytes under control of the simplified GFAP promoter version gfaABC 1 D. (B) Astrocytes expressed EGFP cell type-specifically after infection with LVGFAPEGFP in a neuron-glia coculture (DIV 22). Cultures were treated for 4 h with either 50 ng/ml BDNF or 2 μM TTX in the presence of 4 mM AHA and processed for 'click reaction' with a TAMRA-alkyne tag in the FUNCAT procedure. BDNF application led to an upregulation of TAMRA signal intensities up to 150%. TAMRA intensities are color coded in the lower panel (scale bar = 10 μm). (C) GFAP-positive astrocytes in monoculture (DIV 20–22) expressing EGFP show fibroblast-like morphology. No differences in TAMRA signal intensities were observed when cultures were treated analogous to (B) (scale bar = 10 μm). (D) Cultures were manipulated as described in (B). Mean TAMRA signal intensities were determined within the EGFP mask. A significant increase in TAMRA signal intensities was detected after BDNF treatment. Numbers at the X-axis indicate the number of cells included in the quantification (3 independent experiments; represented data are mean +/- SEM; ONE-way ANOVA, ***: p

    Techniques Used: Expressing, Plasmid Preparation, Infection

    2) Product Images from "Prefrontal BDNF Levels After Anodal Epidural Direct Current Stimulation in Rats"

    Article Title: Prefrontal BDNF Levels After Anodal Epidural Direct Current Stimulation in Rats

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2018.00755

    Western blot analysis of brain-derived neurotrophic factor (BDNF) in the prefrontal cortex (PFC) after five (once a day) consecutive sessions of sham (R-sham, n = 4) or epidural direct current stimulation (R-eDCS, n = 4) at 400 μA current intensity during 11 min through a 5-mm round electrode implanted over the left medial Prefrontal Cortex (mPFC). A diagram of general procedures is shown at the top. (A) Bands of the immunoreactivity related to the migration of the proBDNF and mBDNF shown in two animals – biological replicate – from each group; Densitometry analysis for (B) Total BDNF (intensity of proBDNF plus mBDNF), (C) proBDNF, and (D) mBDNF. Densitometry analyses were normalized to GAPDH and values are presented as the mean ± SEM. ∗∗ p
    Figure Legend Snippet: Western blot analysis of brain-derived neurotrophic factor (BDNF) in the prefrontal cortex (PFC) after five (once a day) consecutive sessions of sham (R-sham, n = 4) or epidural direct current stimulation (R-eDCS, n = 4) at 400 μA current intensity during 11 min through a 5-mm round electrode implanted over the left medial Prefrontal Cortex (mPFC). A diagram of general procedures is shown at the top. (A) Bands of the immunoreactivity related to the migration of the proBDNF and mBDNF shown in two animals – biological replicate – from each group; Densitometry analysis for (B) Total BDNF (intensity of proBDNF plus mBDNF), (C) proBDNF, and (D) mBDNF. Densitometry analyses were normalized to GAPDH and values are presented as the mean ± SEM. ∗∗ p

    Techniques Used: Western Blot, Derivative Assay, Migration

    Western blot analysis of brain-derived neurotrophic factor (BDNF) in the prefrontal cortex (PFC) 15 ( n = 4), 30 ( n = 4) or 60 ( n = 4) minutes after a single session of epidural direct current stimulation (S-eDCS) at 400 μA current intensity during 11 min through a 5-mm round electrode implanted over the left medial Prefrontal Cortex (mPFC) or sham procedure (S-sham). A diagram of general procedures is shown at the top. (A) Bands of the immunoreactivity related to the migration of the proBDNF and mBDNF shown in two animals – biological replicate – from each group. Densitometry analysis: (B) Total BDNF (intensity of proBDNF plus mBDNF), (C) proBDNF, and, (D) mBDNF. Densitometry analyses were normalized to GAPDH and values are presented as the mean ± SEM. ∗ p
    Figure Legend Snippet: Western blot analysis of brain-derived neurotrophic factor (BDNF) in the prefrontal cortex (PFC) 15 ( n = 4), 30 ( n = 4) or 60 ( n = 4) minutes after a single session of epidural direct current stimulation (S-eDCS) at 400 μA current intensity during 11 min through a 5-mm round electrode implanted over the left medial Prefrontal Cortex (mPFC) or sham procedure (S-sham). A diagram of general procedures is shown at the top. (A) Bands of the immunoreactivity related to the migration of the proBDNF and mBDNF shown in two animals – biological replicate – from each group. Densitometry analysis: (B) Total BDNF (intensity of proBDNF plus mBDNF), (C) proBDNF, and, (D) mBDNF. Densitometry analyses were normalized to GAPDH and values are presented as the mean ± SEM. ∗ p

    Techniques Used: Western Blot, Derivative Assay, Migration

    3) Product Images from "Brain-Derived Neurotrophic Factor-Dependent cdk1 Inhibition Prevents G2/M Progression in Differentiating Tetraploid Neurons"

    Article Title: Brain-Derived Neurotrophic Factor-Dependent cdk1 Inhibition Prevents G2/M Progression in Differentiating Tetraploid Neurons

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064890

    BDNF reduces cdk1 protein levels in DCRNs. ( A ) Lysates from DCRNs cultured for 20 h in the presence of different combinations of 100 ng/m NGF and 2 ng/ml BDNF were subjected to western blot with antibodies specific for either cdk1 (upper panel) or β-actin (lower panel). ( B ) Normalized cdk1/β-actin ratio. ( C ) Semiquantitative RT-PCR analysis of mRNA samples obtained from DCRNs cultured for 20 h in the presence of either vehicle (Control) or 2 ng/ml BDNF (BDNF). The levels of Cdk1 expression were normalized to Gapdh . N.S. non-significant; *p
    Figure Legend Snippet: BDNF reduces cdk1 protein levels in DCRNs. ( A ) Lysates from DCRNs cultured for 20 h in the presence of different combinations of 100 ng/m NGF and 2 ng/ml BDNF were subjected to western blot with antibodies specific for either cdk1 (upper panel) or β-actin (lower panel). ( B ) Normalized cdk1/β-actin ratio. ( C ) Semiquantitative RT-PCR analysis of mRNA samples obtained from DCRNs cultured for 20 h in the presence of either vehicle (Control) or 2 ng/ml BDNF (BDNF). The levels of Cdk1 expression were normalized to Gapdh . N.S. non-significant; *p

    Techniques Used: Cell Culture, Western Blot, Reverse Transcription Polymerase Chain Reaction, Expressing

    Post-transductional effects of BDNF on cdk1 function. ( A ) E6 retinal cells electroporated with either EGFP (-) or cdk1 plus cyclin B1 (CC) and EGFP (+) were cultured under neurogenic conditions for 20 h in the presence of different combinations of 100 ng/ml NGF and 2 ng/ml BDNF. The percentage of mitotic figures was evaluated in the EGFP-positive cells. Lower panels show an example of a mitotic figure (pH3; red) in an EGFP-transfected cell (EGFP) (arrow). ( B ) E6 retinal cells were electroporated and cultured as above. The percentage of pyknotic nuclei was evaluated in the EGFP-positive cells. Lower panels show an example of a pyknotic nucleus in an EGFP-transfected cell (EGFP) (arrow). Bisb.: bisbenzimide. *p
    Figure Legend Snippet: Post-transductional effects of BDNF on cdk1 function. ( A ) E6 retinal cells electroporated with either EGFP (-) or cdk1 plus cyclin B1 (CC) and EGFP (+) were cultured under neurogenic conditions for 20 h in the presence of different combinations of 100 ng/ml NGF and 2 ng/ml BDNF. The percentage of mitotic figures was evaluated in the EGFP-positive cells. Lower panels show an example of a mitotic figure (pH3; red) in an EGFP-transfected cell (EGFP) (arrow). ( B ) E6 retinal cells were electroporated and cultured as above. The percentage of pyknotic nuclei was evaluated in the EGFP-positive cells. Lower panels show an example of a pyknotic nucleus in an EGFP-transfected cell (EGFP) (arrow). Bisb.: bisbenzimide. *p

    Techniques Used: Cell Culture, Transfection

    Post-transductional effects of BDNF on cdk1 function are specific for Tyr15. E6 retinal cells electroporated with either EGFP (-) or the Tyr15Phe mutant form of cdk1 plus cyclin B1 (Mut CC) and EGFP (+) were cultured for 20 h under neurogenic conditions in the presence of different combinations of 100 ng/ml NGF and 2 ng/m BDNF. The percentage of pyknotic nuclei was evaluated in EGFP-positive cells. *p
    Figure Legend Snippet: Post-transductional effects of BDNF on cdk1 function are specific for Tyr15. E6 retinal cells electroporated with either EGFP (-) or the Tyr15Phe mutant form of cdk1 plus cyclin B1 (Mut CC) and EGFP (+) were cultured for 20 h under neurogenic conditions in the presence of different combinations of 100 ng/ml NGF and 2 ng/m BDNF. The percentage of pyknotic nuclei was evaluated in EGFP-positive cells. *p

    Techniques Used: Mutagenesis, Cell Culture

    BDNF induces Tyr15 phosphorylation in cdk1. ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or K252a were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p
    Figure Legend Snippet: BDNF induces Tyr15 phosphorylation in cdk1. ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or K252a were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p

    Techniques Used: Sandwich ELISA, Sequencing, Cell Culture, Incubation

    Cdk1 protein kinase activity measured from DCRNs in either the presence or absence of BDNF. ( A ) General cdk protein kinase activity, based on the phosphorylation of a Rb-derived peptide by the cdk activity present in cell extracts from DCRNs cultured for 20 h in the presence of 100 ng/ml NGF and treated for 30 min with either vehicle (Control) or 2 ng/ml BDNF. Equal amount of cell extracts were used in both cases. ( B ) Left panel, a representative western blot performed with an anti-cdk1 antibody using the cell extracts described above prior to immunoprecipitation (INPUT). Right panel, protein kinase activity immunoprecipitated with an anti cdk1 antibody (i.e. cdk1-specific kinase activity) from the cell extracts described above, normalized to the relative amount of cdk1 present in these extracts (see input in left panel). *p
    Figure Legend Snippet: Cdk1 protein kinase activity measured from DCRNs in either the presence or absence of BDNF. ( A ) General cdk protein kinase activity, based on the phosphorylation of a Rb-derived peptide by the cdk activity present in cell extracts from DCRNs cultured for 20 h in the presence of 100 ng/ml NGF and treated for 30 min with either vehicle (Control) or 2 ng/ml BDNF. Equal amount of cell extracts were used in both cases. ( B ) Left panel, a representative western blot performed with an anti-cdk1 antibody using the cell extracts described above prior to immunoprecipitation (INPUT). Right panel, protein kinase activity immunoprecipitated with an anti cdk1 antibody (i.e. cdk1-specific kinase activity) from the cell extracts described above, normalized to the relative amount of cdk1 present in these extracts (see input in left panel). *p

    Techniques Used: Activity Assay, Derivative Assay, Cell Culture, Western Blot, Immunoprecipitation

    4) Product Images from "A Chinese herbal decoction, reformulated from Kai-Xin-San, relieves the depression-like symptoms in stressed rats and induces neurogenesis in cultured neurons"

    Article Title: A Chinese herbal decoction, reformulated from Kai-Xin-San, relieves the depression-like symptoms in stressed rats and induces neurogenesis in cultured neurons

    Journal: Scientific Reports

    doi: 10.1038/srep30014

    KXS 2012 promotes neurogenesis in cultured neurons. ( A ) KXS 2012 (0.3 to 3 μg/mL) was applied onto cultured rat cortical neurons (DIV 5) for 96 h. The protein levels of PSD-95 (~95 kDa) and synaptotagmin (~60 kDa) were measured by Western blot assay (upper panel). α-Tubulin (~55 kDa) served as a loading control. Forskolin (Fsk, 50 nM) was used as a positive control. The amount of protein level was quantified (lower panel). (B ) KXS 2012 (3 μg/mL) and BDNF (15 ng/mL) were applied onto cultured hippocampal neurons (DIV 15) for 96 h before pEGFP-N1 transfection. The treatment significantly increased the spine density of control neurons (9–16 dendrites from 6–10 neurons per 10 μm were measured for each condition). The quantification plot was shown in lower right. (C) Representative images show that KXS 2012 (3 μg/mL) and BDNF (15 ng/mL) increased the spine density compared to control. The counted spine density was indicated by asterisk. Scale bars = 10 μm. Values are expressed the fold of change as compared to control (x Basal) or number, Mean ± SEM, n = 4–10. * p
    Figure Legend Snippet: KXS 2012 promotes neurogenesis in cultured neurons. ( A ) KXS 2012 (0.3 to 3 μg/mL) was applied onto cultured rat cortical neurons (DIV 5) for 96 h. The protein levels of PSD-95 (~95 kDa) and synaptotagmin (~60 kDa) were measured by Western blot assay (upper panel). α-Tubulin (~55 kDa) served as a loading control. Forskolin (Fsk, 50 nM) was used as a positive control. The amount of protein level was quantified (lower panel). (B ) KXS 2012 (3 μg/mL) and BDNF (15 ng/mL) were applied onto cultured hippocampal neurons (DIV 15) for 96 h before pEGFP-N1 transfection. The treatment significantly increased the spine density of control neurons (9–16 dendrites from 6–10 neurons per 10 μm were measured for each condition). The quantification plot was shown in lower right. (C) Representative images show that KXS 2012 (3 μg/mL) and BDNF (15 ng/mL) increased the spine density compared to control. The counted spine density was indicated by asterisk. Scale bars = 10 μm. Values are expressed the fold of change as compared to control (x Basal) or number, Mean ± SEM, n = 4–10. * p

    Techniques Used: Cell Culture, Western Blot, Positive Control, Transfection

    5) Product Images from "Positive Feedback Regulation between ?-Aminobutyric Acid Type A (GABAA) Receptor Signaling and Brain-derived Neurotrophic Factor (BDNF) Release in Developing Neurons *"

    Article Title: Positive Feedback Regulation between ?-Aminobutyric Acid Type A (GABAA) Receptor Signaling and Brain-derived Neurotrophic Factor (BDNF) Release in Developing Neurons *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.201582

    GABA A R activation induces BDNF/TrkB-dependent phosphorylation of CREB. A , immunofluorescence of CREB ( left panels ; red ) and pCREB ( middle panels ; green ) in cortical neurons in control conditions ( Ctrl ) or treated with muscimol alone (50 μ m ; Musc
    Figure Legend Snippet: GABA A R activation induces BDNF/TrkB-dependent phosphorylation of CREB. A , immunofluorescence of CREB ( left panels ; red ) and pCREB ( middle panels ; green ) in cortical neurons in control conditions ( Ctrl ) or treated with muscimol alone (50 μ m ; Musc

    Techniques Used: Activation Assay, Immunofluorescence

    GABA A R-dependent release of endogenous BDNF mediates increase in cell surface expression. A , cultured cerebrocortical neurons (6 DIV) in control conditions ( Ctrl ) or treated with muscimol in the absence ( Musc ) or presence of TrkB-IgG ( Musc + TrkB-IgG
    Figure Legend Snippet: GABA A R-dependent release of endogenous BDNF mediates increase in cell surface expression. A , cultured cerebrocortical neurons (6 DIV) in control conditions ( Ctrl ) or treated with muscimol in the absence ( Musc ) or presence of TrkB-IgG ( Musc + TrkB-IgG

    Techniques Used: Expressing, Cell Culture

    6) Product Images from "Postsynaptic Spiking Homeostatically Induces Cell-Autonomous Regulation of Inhibitory Inputs via Retrograde Signaling"

    Article Title: Postsynaptic Spiking Homeostatically Induces Cell-Autonomous Regulation of Inhibitory Inputs via Retrograde Signaling

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.3085-10.2010

    In vivo kainic acid injection increased neuronal spiking, BDNF level and inhibitory synaptic transmission in juvenile rats. A , Representative recordings of spontaneous firing activity from CA1 pyramidal neurons in hippocampal slices for conditions as indicated, the scale bars are 20 mV and 5 s. B , Average firing rates from Ctrl (1.42 ± 0.40 Hz) rats, and those injected with KA for 1 h (2.58 ± 0.54 Hz, p > 0.05) or 4 h (4.05 ± 0.69 Hz, p
    Figure Legend Snippet: In vivo kainic acid injection increased neuronal spiking, BDNF level and inhibitory synaptic transmission in juvenile rats. A , Representative recordings of spontaneous firing activity from CA1 pyramidal neurons in hippocampal slices for conditions as indicated, the scale bars are 20 mV and 5 s. B , Average firing rates from Ctrl (1.42 ± 0.40 Hz) rats, and those injected with KA for 1 h (2.58 ± 0.54 Hz, p > 0.05) or 4 h (4.05 ± 0.69 Hz, p

    Techniques Used: In Vivo, Injection, Transmission Assay, Activity Assay

    7) Product Images from "NMDA-Dependent Switch of proBDNF Actions on Developing GABAergic Synapses"

    Article Title: NMDA-Dependent Switch of proBDNF Actions on Developing GABAergic Synapses

    Journal: Cerebral Cortex (New York, NY)

    doi: 10.1093/cercor/bhs071

    The mBDNF-TrkB receptor signaling is not impaired in the presence of aprotinin. ( A, B ) Mean native BDNF mRNA ( A , 4 intact hippocampi for each condition) and protein ( B , 4 slices for each condition) levels in control ACSF or aprotinin-treated tissue. (
    Figure Legend Snippet: The mBDNF-TrkB receptor signaling is not impaired in the presence of aprotinin. ( A, B ) Mean native BDNF mRNA ( A , 4 intact hippocampi for each condition) and protein ( B , 4 slices for each condition) levels in control ACSF or aprotinin-treated tissue. (

    Techniques Used:

    8) Product Images from "Brain-derived neurotrophic factor increases expression of MnSOD in human circulating angiogenic cells"

    Article Title: Brain-derived neurotrophic factor increases expression of MnSOD in human circulating angiogenic cells

    Journal: Microvascular Research

    doi: 10.1016/j.mvr.2012.01.001

    BDNF increased MnSOD expression in EPCs. A and B: Human EPCs were treated with BDNF (50 or 100 ng/ml) in EBM2 for 24 h. Protein samples were subjected to Western blotting. Blots are representative of 3 independent experiments. B: Optical density analysis of MnSOD protein levels; n=3, *P
    Figure Legend Snippet: BDNF increased MnSOD expression in EPCs. A and B: Human EPCs were treated with BDNF (50 or 100 ng/ml) in EBM2 for 24 h. Protein samples were subjected to Western blotting. Blots are representative of 3 independent experiments. B: Optical density analysis of MnSOD protein levels; n=3, *P

    Techniques Used: Expressing, Western Blot

    Phenotyping of EPCs. Mononuclear cells were cultured on fibronectin-coated plates in EGM2 for 4 days. A: Phase contrast image of day 4 EPCs (× 20 magnification). B–E: FACS analysis of cell surface markers on EPCs. Shown are representative data from at least 3 independent experiments for each marker. The open black-lined histograms represent tested antibodies, and filled histograms represent the control IgG. F: Human EPCs expressed receptors that bind to BDNF, TrkB and p75 NTR . Positive controls (Post) for p75NTR and TrkB are SK-N-MC cell lysate and mouse brain, respectively. G: mRNA expressions of TrkB and p75 NTR in day 4 EPCs and circulating mononuclear cells (MNC).
    Figure Legend Snippet: Phenotyping of EPCs. Mononuclear cells were cultured on fibronectin-coated plates in EGM2 for 4 days. A: Phase contrast image of day 4 EPCs (× 20 magnification). B–E: FACS analysis of cell surface markers on EPCs. Shown are representative data from at least 3 independent experiments for each marker. The open black-lined histograms represent tested antibodies, and filled histograms represent the control IgG. F: Human EPCs expressed receptors that bind to BDNF, TrkB and p75 NTR . Positive controls (Post) for p75NTR and TrkB are SK-N-MC cell lysate and mouse brain, respectively. G: mRNA expressions of TrkB and p75 NTR in day 4 EPCs and circulating mononuclear cells (MNC).

    Techniques Used: Cell Culture, FACS, Marker

    BDNF induced phosphorylation of IKKα/β, and JNK. A–C: EPCs were cultured in EBM-2 for 20 h, then were treated with BDNF for indicated periods. All blots are representative of at least 3 independent experiments. A: n=6–9, P
    Figure Legend Snippet: BDNF induced phosphorylation of IKKα/β, and JNK. A–C: EPCs were cultured in EBM-2 for 20 h, then were treated with BDNF for indicated periods. All blots are representative of at least 3 independent experiments. A: n=6–9, P

    Techniques Used: Cell Culture

    BDNF protected EPCs from oxidative stress. A and B: EPCs were incubated with indicated treatments, then were subjected to TUNEL assay (A, n=5–8, *P
    Figure Legend Snippet: BDNF protected EPCs from oxidative stress. A and B: EPCs were incubated with indicated treatments, then were subjected to TUNEL assay (A, n=5–8, *P

    Techniques Used: Incubation, TUNEL Assay

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    • mature bdnf  (Alomone Labs)
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    Alomone Labs mature bdnf
    Line-blot for qualitative analysis of <t>anti-BDNF</t> antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D <t>System-Quantikine</t> ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.
    Mature Bdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mature bdnf/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    recombinant human bdnf protein  (Alomone Labs)
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    Alomone Labs recombinant human bdnf protein
    Rearing larvae in dim light reduces production of <t>BDNF</t> in the medial PGZ by 10 <t>dpf</t> ( A–C ). Scale bar, 15 μm. Injecting 5 dpf larvae with 100 ng/ml recombinant human BDNF increased the amount of BDNF protein detected by immunohistochemistry
    Recombinant Human Bdnf Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
    recombinant human bdnf protein - by Bioz Stars, 2022-08
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    Line-blot for qualitative analysis of anti-BDNF antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.

    Journal: Scientific Reports

    Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

    doi: 10.1038/srep17989

    Figure Lengend Snippet: Line-blot for qualitative analysis of anti-BDNF antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.

    Article Snippet: Of note, Aviscera-Bioscience and R & D System-Quantikine® kits were claimed to be specific for mature BDNF and to have some cross-reactivity with the human pro-BDNF.

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Dot Blot

    Effects of TrkB-Fc and pro-BDNF on PTZ-induced kindling in wild-type and MMP-9 (−/−) mice. A , B , Mice received microinjections of TrkB-Fc or IgG 1 -Fc into the right ventricle 30 min before each PTZ treatment (25 mg/kg). Values are means ± SE ( n = 10 for A ; n = 8 for B ). * p

    Journal: The Journal of Neuroscience

    Article Title: Matrix Metalloproteinase-9 Contributes to Kindled Seizure Development in Pentylenetetrazole-Treated Mice by Converting Pro-BDNF to Mature BDNF in the Hippocampus

    doi: 10.1523/JNEUROSCI.3118-11.2011

    Figure Lengend Snippet: Effects of TrkB-Fc and pro-BDNF on PTZ-induced kindling in wild-type and MMP-9 (−/−) mice. A , B , Mice received microinjections of TrkB-Fc or IgG 1 -Fc into the right ventricle 30 min before each PTZ treatment (25 mg/kg). Values are means ± SE ( n = 10 for A ; n = 8 for B ). * p

    Article Snippet: Our findings suggest that MMP-9 plays a role in kindling development by converting pro-BDNF to mature BDNF in the hippocampus, whereas other factors mediate BDNF maturation during the later kindling stage.

    Techniques: Mouse Assay

    Rearing larvae in dim light reduces production of BDNF in the medial PGZ by 10 dpf ( A–C ). Scale bar, 15 μm. Injecting 5 dpf larvae with 100 ng/ml recombinant human BDNF increased the amount of BDNF protein detected by immunohistochemistry

    Journal: The Journal of Neuroscience

    Article Title: Visual Experience Facilitates BDNF-Dependent Adaptive Recruitment of New Neurons in the Postembryonic Optic Tectum

    doi: 10.1523/JNEUROSCI.1962-17.2018

    Figure Lengend Snippet: Rearing larvae in dim light reduces production of BDNF in the medial PGZ by 10 dpf ( A–C ). Scale bar, 15 μm. Injecting 5 dpf larvae with 100 ng/ml recombinant human BDNF increased the amount of BDNF protein detected by immunohistochemistry

    Article Snippet: To test whether exogenous BDNF was detectable in whole-brain lysates following ICV injections, we performed a Western blot using 5 dpf larvae injected with either 100 ng/ml recombinant human BDNF protein (#B-250, Alomone Labs) or a vehicle control.

    Techniques: Recombinant, Immunohistochemistry

    Structural plasticity induced by nicotine in mesencephalic DA neurons from wild-type (WT) mice and α6 or α4/α6 nAChR subunit null mutant mice. Morphological effects of nicotine on DA neurons from (A–C) α6KO and WT mice and (D–F) α4/α6KO and WT mice on maximal dendrite length (A,D) , number of primary dendrites (B,E) and soma area (C,F) measured 72 h after exposure to nicotine (10 μM), or BDNF (10 ng/ml) here used as active control group. Two-way ANOVA was used to analyze data in panels (A–C) and (D–F) , respectively. Data are expressed as mean ± SEM ( ∗∗∗ p

    Journal: Frontiers in Pharmacology

    Article Title: Alpha6-Containing Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Structural Plasticity in Mouse and Human iPSC-Derived Dopaminergic Neurons

    doi: 10.3389/fphar.2018.00572

    Figure Lengend Snippet: Structural plasticity induced by nicotine in mesencephalic DA neurons from wild-type (WT) mice and α6 or α4/α6 nAChR subunit null mutant mice. Morphological effects of nicotine on DA neurons from (A–C) α6KO and WT mice and (D–F) α4/α6KO and WT mice on maximal dendrite length (A,D) , number of primary dendrites (B,E) and soma area (C,F) measured 72 h after exposure to nicotine (10 μM), or BDNF (10 ng/ml) here used as active control group. Two-way ANOVA was used to analyze data in panels (A–C) and (D–F) , respectively. Data are expressed as mean ± SEM ( ∗∗∗ p

    Article Snippet: The (-)-nicotine ditartrate (Tocris Bioscience, Bristol, United Kingdom), brain-derived neurotrophic factor (BDNF) (Alomone Labs Ltd., Jerusalem, Israel) and nAChR antagonists α-conotoxin MII (α-CTX MII), α-conotoxin PIA (α-CTX PIA), mecamylamine (MEC), dihydro-β-erythroidine (DHβE), and methyllycaconitine (MLA) (Tocris Bioscience) were used in the present study and are detailed in Supplementary Table .

    Techniques: Mouse Assay, Mutagenesis