Journal: BMC Cancer
Article Title: Opening large-conductance potassium channels selectively induced cell death of triple-negative breast cancer
Figure Lengend Snippet: KCNMA1 gene expression in five molecular subtypes of breast cancer. Each dot represents one patient. Gene expression levels are presented as FPKM in log2 expression. Red dots are basal-like triple-negative (TN) breast cancer patients. Blue dotted line indicates KCNMA1 gene expression in normal breast cells. FPKM: Fragments Per Kilobase of transcript per Million
Article Snippet: Next, blots were blocked in Licor blocking buffer for one hour, and incubated for 12 h at 4 degrees with primary antibody for either anti-KCNMA1 for epitope 199–213 (Alomone cat# APC-151), anti-caspase-3 (Cell Signaling Technology), or anti-Slo1 for c-terminus segment 9–10 (Millipore) at 1:500 dilution.