cx26  (Alomone Labs)


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    Alomone Labs cx26
    Expression profile of CX45, CX36, and <t>CX26</t> transcripts in the human fetal cortex, from 17 to 23 GW. ( A ) Log10 FPKM values derived by the HISAT-StringTie pathway of data analysis. ( B ) Log10 normalized counts derived by the STAR-HTSeq-DESeq2 data analysis pathway. ( C ) Expression profile of 2 predominant transcripts of Cx45 (ENST00000592524.5 [CX45_524.5] and ENST00000426548.5 [Cx45_548.5]) in comparison with Cx36 and Cx26 with HISAT-StringTie pathway of analysis from 17 to 23 GW in the human fetal cortex. In panels A – C , n = 4, 17 GW; n = 2, 18 GW; n = 5, 19 GW; and n = 1, 23 GW. ( D ) Log10 normalized counts of the Cx and pannexin isoforms expressed in the human fetal cortex, age range of 17–23 GW (STAR-HTSeq-DESeq2 data analysis pathway) ( n = 7). ( E ) Differential expression analysis of human fetal samples at 17 and 19 GW, by the STAR-HTSeq-DESeq2 method ( n = 3, 17 GW; n = 2, 19 GW). ( F ) Sex determination based on XIST expression and ratio of X-to-Y coverage in each human subject used in the study ( n = 7). ( G ) Differential expression analysis of male ( n = 4) and female ( n = 3) human fetal samples from 17 to 19 GW, by the STAR-HTSeq-DESeq2 method. ( H ) Differential expression analysis of cortex ( n = 3) and full brain ( n = 3) human fetal samples at 17 GW by the STAR-HTSeq-DESeq2 method. * P adj
    Cx26, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cx26/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cx26 - by Bioz Stars, 2022-11
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    Images

    1) Product Images from "Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex"

    Article Title: Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex

    Journal: Cerebral Cortex (New York, NY)

    doi: 10.1093/cercor/bhz163

    Expression profile of CX45, CX36, and CX26 transcripts in the human fetal cortex, from 17 to 23 GW. ( A ) Log10 FPKM values derived by the HISAT-StringTie pathway of data analysis. ( B ) Log10 normalized counts derived by the STAR-HTSeq-DESeq2 data analysis pathway. ( C ) Expression profile of 2 predominant transcripts of Cx45 (ENST00000592524.5 [CX45_524.5] and ENST00000426548.5 [Cx45_548.5]) in comparison with Cx36 and Cx26 with HISAT-StringTie pathway of analysis from 17 to 23 GW in the human fetal cortex. In panels A – C , n = 4, 17 GW; n = 2, 18 GW; n = 5, 19 GW; and n = 1, 23 GW. ( D ) Log10 normalized counts of the Cx and pannexin isoforms expressed in the human fetal cortex, age range of 17–23 GW (STAR-HTSeq-DESeq2 data analysis pathway) ( n = 7). ( E ) Differential expression analysis of human fetal samples at 17 and 19 GW, by the STAR-HTSeq-DESeq2 method ( n = 3, 17 GW; n = 2, 19 GW). ( F ) Sex determination based on XIST expression and ratio of X-to-Y coverage in each human subject used in the study ( n = 7). ( G ) Differential expression analysis of male ( n = 4) and female ( n = 3) human fetal samples from 17 to 19 GW, by the STAR-HTSeq-DESeq2 method. ( H ) Differential expression analysis of cortex ( n = 3) and full brain ( n = 3) human fetal samples at 17 GW by the STAR-HTSeq-DESeq2 method. * P adj
    Figure Legend Snippet: Expression profile of CX45, CX36, and CX26 transcripts in the human fetal cortex, from 17 to 23 GW. ( A ) Log10 FPKM values derived by the HISAT-StringTie pathway of data analysis. ( B ) Log10 normalized counts derived by the STAR-HTSeq-DESeq2 data analysis pathway. ( C ) Expression profile of 2 predominant transcripts of Cx45 (ENST00000592524.5 [CX45_524.5] and ENST00000426548.5 [Cx45_548.5]) in comparison with Cx36 and Cx26 with HISAT-StringTie pathway of analysis from 17 to 23 GW in the human fetal cortex. In panels A – C , n = 4, 17 GW; n = 2, 18 GW; n = 5, 19 GW; and n = 1, 23 GW. ( D ) Log10 normalized counts of the Cx and pannexin isoforms expressed in the human fetal cortex, age range of 17–23 GW (STAR-HTSeq-DESeq2 data analysis pathway) ( n = 7). ( E ) Differential expression analysis of human fetal samples at 17 and 19 GW, by the STAR-HTSeq-DESeq2 method ( n = 3, 17 GW; n = 2, 19 GW). ( F ) Sex determination based on XIST expression and ratio of X-to-Y coverage in each human subject used in the study ( n = 7). ( G ) Differential expression analysis of male ( n = 4) and female ( n = 3) human fetal samples from 17 to 19 GW, by the STAR-HTSeq-DESeq2 method. ( H ) Differential expression analysis of cortex ( n = 3) and full brain ( n = 3) human fetal samples at 17 GW by the STAR-HTSeq-DESeq2 method. * P adj

    Techniques Used: Expressing, Derivative Assay

    Cells expressing Cx26 in 3 zones of cortical mantle. ( A ) Human fetal cortex (20 GW, female). Hoechst stain. Composite (mosaic) of multiple images. ( B ) CP, ( C ) SP, and ( D ) SVZ. Green = neuronal marker, Neun; red = Cx isoform, Cx26; blue = nuclear stain by Hoechst. Scales in panels B – D , 20 μm.
    Figure Legend Snippet: Cells expressing Cx26 in 3 zones of cortical mantle. ( A ) Human fetal cortex (20 GW, female). Hoechst stain. Composite (mosaic) of multiple images. ( B ) CP, ( C ) SP, and ( D ) SVZ. Green = neuronal marker, Neun; red = Cx isoform, Cx26; blue = nuclear stain by Hoechst. Scales in panels B – D , 20 μm.

    Techniques Used: Expressing, Staining, Marker

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    Alomone Labs anti cx26 antibody
    Inhibition of dye uptake by the agents CVB2-61 or CVB4-57. The rate of dye uptake in <t>Cx26</t> ( a , b ) or Cx46 ( c , d ) expressing N2A cells was measured in Ca 2+ free external solution in the presence or absence of the indicated concentrations of CVB2-61 ( a , c ) and CVB4-57 ( b , d ). Representative experiments indicating the time course of the dye uptake in the presence of the compounds are given in Figure S3 . The data were normalised to the mean rate of dye uptake found in N2A cells expressing Cxs after removal of external Ca 2+ and in the absence of the agents. The data are given as mean ± SEM for at least three respective transfections. The number of cells used for each experiment is given as n. The lines were obtained by fitting the means to a single exponential function. One-way ANOVA was applied to estimate the statistical significance to the 0 µM treated cells ( p : *
    Anti Cx26 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 2 article reviews
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    anti cx26 antibody - by Bioz Stars, 2022-11
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    Inhibition of dye uptake by the agents CVB2-61 or CVB4-57. The rate of dye uptake in Cx26 ( a , b ) or Cx46 ( c , d ) expressing N2A cells was measured in Ca 2+ free external solution in the presence or absence of the indicated concentrations of CVB2-61 ( a , c ) and CVB4-57 ( b , d ). Representative experiments indicating the time course of the dye uptake in the presence of the compounds are given in Figure S3 . The data were normalised to the mean rate of dye uptake found in N2A cells expressing Cxs after removal of external Ca 2+ and in the absence of the agents. The data are given as mean ± SEM for at least three respective transfections. The number of cells used for each experiment is given as n. The lines were obtained by fitting the means to a single exponential function. One-way ANOVA was applied to estimate the statistical significance to the 0 µM treated cells ( p : *

    Journal: Pharmaceuticals

    Article Title: The Bioactive Phenolic Agents Diaryl Ether CVB2-61 and Diarylheptanoid CVB4-57 as Connexin Hemichannel Blockers

    doi: 10.3390/ph15101173

    Figure Lengend Snippet: Inhibition of dye uptake by the agents CVB2-61 or CVB4-57. The rate of dye uptake in Cx26 ( a , b ) or Cx46 ( c , d ) expressing N2A cells was measured in Ca 2+ free external solution in the presence or absence of the indicated concentrations of CVB2-61 ( a , c ) and CVB4-57 ( b , d ). Representative experiments indicating the time course of the dye uptake in the presence of the compounds are given in Figure S3 . The data were normalised to the mean rate of dye uptake found in N2A cells expressing Cxs after removal of external Ca 2+ and in the absence of the agents. The data are given as mean ± SEM for at least three respective transfections. The number of cells used for each experiment is given as n. The lines were obtained by fitting the means to a single exponential function. One-way ANOVA was applied to estimate the statistical significance to the 0 µM treated cells ( p : *

    Article Snippet: The primary anti-Cx46 antibody SantaCruz Biotechnology, Inc., Heidelberg, Germany, sc-365394) and anti-Cx26 antibody; (Alomone labs, Jerusalem, Israel ACC-212) were used.

    Techniques: Inhibition, Expressing, Transfection

    Dye uptake in N2A cells expressing connexins. ( a ) The time course of the fluorescence intensity of Etd in N2A cells transfected with GFP vector (Contr), hCx26IRES-GFP (Cx26) vector, or Cx46IRES-GFP vector (Cx46). The dots show the fluorescence intensity measured in single cells expressing only GFP (grey), Cx26 (black), and Cx46 (blue) in the presence and absence of external Ca 2+ . Removal of external Ca 2+ led to a rapid increase in the measured fluorescence intensity in the cells expressing Cxs, but not in the control cells, indicating an opening of the Cx hemichannels. The lines show a linear regression along the fluorescence intensity in the presence and absence of external Ca 2+ ; the slope was used to estimate the rate of Etd uptake. ( b ) Rate of Etd uptake in N2A cells transfected with the Cx isoforms compared to cells expressing GFP alone (Contr). The results are given as mean ± SD of at least three respective transfections. The number of analysed cells is given as n. One-way ANOVA was applied to estimate the statistical significance ( p : #

    Journal: Pharmaceuticals

    Article Title: The Bioactive Phenolic Agents Diaryl Ether CVB2-61 and Diarylheptanoid CVB4-57 as Connexin Hemichannel Blockers

    doi: 10.3390/ph15101173

    Figure Lengend Snippet: Dye uptake in N2A cells expressing connexins. ( a ) The time course of the fluorescence intensity of Etd in N2A cells transfected with GFP vector (Contr), hCx26IRES-GFP (Cx26) vector, or Cx46IRES-GFP vector (Cx46). The dots show the fluorescence intensity measured in single cells expressing only GFP (grey), Cx26 (black), and Cx46 (blue) in the presence and absence of external Ca 2+ . Removal of external Ca 2+ led to a rapid increase in the measured fluorescence intensity in the cells expressing Cxs, but not in the control cells, indicating an opening of the Cx hemichannels. The lines show a linear regression along the fluorescence intensity in the presence and absence of external Ca 2+ ; the slope was used to estimate the rate of Etd uptake. ( b ) Rate of Etd uptake in N2A cells transfected with the Cx isoforms compared to cells expressing GFP alone (Contr). The results are given as mean ± SD of at least three respective transfections. The number of analysed cells is given as n. One-way ANOVA was applied to estimate the statistical significance ( p : #

    Article Snippet: The primary anti-Cx46 antibody SantaCruz Biotechnology, Inc., Heidelberg, Germany, sc-365394) and anti-Cx26 antibody; (Alomone labs, Jerusalem, Israel ACC-212) were used.

    Techniques: Expressing, Fluorescence, Transfection, Plasmid Preparation

    Expression profile of CX45, CX36, and CX26 transcripts in the human fetal cortex, from 17 to 23 GW. ( A ) Log10 FPKM values derived by the HISAT-StringTie pathway of data analysis. ( B ) Log10 normalized counts derived by the STAR-HTSeq-DESeq2 data analysis pathway. ( C ) Expression profile of 2 predominant transcripts of Cx45 (ENST00000592524.5 [CX45_524.5] and ENST00000426548.5 [Cx45_548.5]) in comparison with Cx36 and Cx26 with HISAT-StringTie pathway of analysis from 17 to 23 GW in the human fetal cortex. In panels A – C , n = 4, 17 GW; n = 2, 18 GW; n = 5, 19 GW; and n = 1, 23 GW. ( D ) Log10 normalized counts of the Cx and pannexin isoforms expressed in the human fetal cortex, age range of 17–23 GW (STAR-HTSeq-DESeq2 data analysis pathway) ( n = 7). ( E ) Differential expression analysis of human fetal samples at 17 and 19 GW, by the STAR-HTSeq-DESeq2 method ( n = 3, 17 GW; n = 2, 19 GW). ( F ) Sex determination based on XIST expression and ratio of X-to-Y coverage in each human subject used in the study ( n = 7). ( G ) Differential expression analysis of male ( n = 4) and female ( n = 3) human fetal samples from 17 to 19 GW, by the STAR-HTSeq-DESeq2 method. ( H ) Differential expression analysis of cortex ( n = 3) and full brain ( n = 3) human fetal samples at 17 GW by the STAR-HTSeq-DESeq2 method. * P adj

    Journal: Cerebral Cortex (New York, NY)

    Article Title: Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex

    doi: 10.1093/cercor/bhz163

    Figure Lengend Snippet: Expression profile of CX45, CX36, and CX26 transcripts in the human fetal cortex, from 17 to 23 GW. ( A ) Log10 FPKM values derived by the HISAT-StringTie pathway of data analysis. ( B ) Log10 normalized counts derived by the STAR-HTSeq-DESeq2 data analysis pathway. ( C ) Expression profile of 2 predominant transcripts of Cx45 (ENST00000592524.5 [CX45_524.5] and ENST00000426548.5 [Cx45_548.5]) in comparison with Cx36 and Cx26 with HISAT-StringTie pathway of analysis from 17 to 23 GW in the human fetal cortex. In panels A – C , n = 4, 17 GW; n = 2, 18 GW; n = 5, 19 GW; and n = 1, 23 GW. ( D ) Log10 normalized counts of the Cx and pannexin isoforms expressed in the human fetal cortex, age range of 17–23 GW (STAR-HTSeq-DESeq2 data analysis pathway) ( n = 7). ( E ) Differential expression analysis of human fetal samples at 17 and 19 GW, by the STAR-HTSeq-DESeq2 method ( n = 3, 17 GW; n = 2, 19 GW). ( F ) Sex determination based on XIST expression and ratio of X-to-Y coverage in each human subject used in the study ( n = 7). ( G ) Differential expression analysis of male ( n = 4) and female ( n = 3) human fetal samples from 17 to 19 GW, by the STAR-HTSeq-DESeq2 method. ( H ) Differential expression analysis of cortex ( n = 3) and full brain ( n = 3) human fetal samples at 17 GW by the STAR-HTSeq-DESeq2 method. * P adj

    Article Snippet: Primary antibodies were purchased from Alomone Labs, Thermo Fisher Scientific, and Millipore (company, antigen [catalog number]: Alomone Labs, Cx26 [ACC-212], Cx36 [ACC-209], and Cx45 [ACC-207]; Thermo Fisher, Cx26 [512800], Cx36 [516200], Nurr1 [MA1-195], beta-3-tubulin [MA1-19582], and NeuN [MA5-33103]; Millipore, NeuN [MAB377]).

    Techniques: Expressing, Derivative Assay

    Cells expressing Cx26 in 3 zones of cortical mantle. ( A ) Human fetal cortex (20 GW, female). Hoechst stain. Composite (mosaic) of multiple images. ( B ) CP, ( C ) SP, and ( D ) SVZ. Green = neuronal marker, Neun; red = Cx isoform, Cx26; blue = nuclear stain by Hoechst. Scales in panels B – D , 20 μm.

    Journal: Cerebral Cortex (New York, NY)

    Article Title: Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex

    doi: 10.1093/cercor/bhz163

    Figure Lengend Snippet: Cells expressing Cx26 in 3 zones of cortical mantle. ( A ) Human fetal cortex (20 GW, female). Hoechst stain. Composite (mosaic) of multiple images. ( B ) CP, ( C ) SP, and ( D ) SVZ. Green = neuronal marker, Neun; red = Cx isoform, Cx26; blue = nuclear stain by Hoechst. Scales in panels B – D , 20 μm.

    Article Snippet: Primary antibodies were purchased from Alomone Labs, Thermo Fisher Scientific, and Millipore (company, antigen [catalog number]: Alomone Labs, Cx26 [ACC-212], Cx36 [ACC-209], and Cx45 [ACC-207]; Thermo Fisher, Cx26 [512800], Cx36 [516200], Nurr1 [MA1-195], beta-3-tubulin [MA1-19582], and NeuN [MA5-33103]; Millipore, NeuN [MAB377]).

    Techniques: Expressing, Staining, Marker

    Epithelial expression of GJB2 and GJB6 in the cochlear duct. (A‐B) The lower basal turn cochlea at W10.4 immunostained for gap junction proteins GJB2 [(A), green] and GJB6 [(B), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (A′) and (B′), respectively. The bracket outlines the prosensory domain. Scale bars = 50 µm. (C‐D) The lower basal turn cochlea at W12.2 immunostained for GJB2 [(C), green] and GJB6 [(D), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (C′) and (D′), respectively. The bracket outlines the prosensory domain. Scale bars = 50 µm. (E‐F) The lower basal turn cochlea at W14 immunostained for GJB2 ((E), green) and GJB6 ((F), green) and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (E′) and (F′), respectively. The bracket outlines the developing organ of Corti. KO = Kölliker's organ. Scale bars = 50 µm. (G–H) The lower basal turn cochlea at W14 immunostained for GJB2 [(G), green] and GJB6 [(H), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (G′) and (H′), respectively. The arrowheads points to the developing root cells. The bracket outlines the developing of Corti. KO = Kölliker's organ. Scale bars = 50 µm. (I‐J) The lower basal turn cochlea at W14 immunostained for GJB2 [(I), green] and GJB6 [(J), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (I′) and (J′), respectively. The arrowheads points to the developing root cells. The bracket outlines the organ of Corti. * = autofluorescent erythrocytes. Scale bars = 50 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com .]

    Journal: Developmental Neurobiology

    Article Title: Development of the stria vascularis and potassium regulation in the human fetal cochlea: Insights into hereditary sensorineural hearing loss

    doi: 10.1002/dneu.22279

    Figure Lengend Snippet: Epithelial expression of GJB2 and GJB6 in the cochlear duct. (A‐B) The lower basal turn cochlea at W10.4 immunostained for gap junction proteins GJB2 [(A), green] and GJB6 [(B), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (A′) and (B′), respectively. The bracket outlines the prosensory domain. Scale bars = 50 µm. (C‐D) The lower basal turn cochlea at W12.2 immunostained for GJB2 [(C), green] and GJB6 [(D), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (C′) and (D′), respectively. The bracket outlines the prosensory domain. Scale bars = 50 µm. (E‐F) The lower basal turn cochlea at W14 immunostained for GJB2 ((E), green) and GJB6 ((F), green) and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (E′) and (F′), respectively. The bracket outlines the developing organ of Corti. KO = Kölliker's organ. Scale bars = 50 µm. (G–H) The lower basal turn cochlea at W14 immunostained for GJB2 [(G), green] and GJB6 [(H), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (G′) and (H′), respectively. The arrowheads points to the developing root cells. The bracket outlines the developing of Corti. KO = Kölliker's organ. Scale bars = 50 µm. (I‐J) The lower basal turn cochlea at W14 immunostained for GJB2 [(I), green] and GJB6 [(J), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (I′) and (J′), respectively. The arrowheads points to the developing root cells. The bracket outlines the organ of Corti. * = autofluorescent erythrocytes. Scale bars = 50 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com .]

    Article Snippet: The primary antibodies used in this study were mouse anti‐acetylated tubulin (aceTUBA, 1:500, T6793, Sigma), rabbit anti‐collagen type IV (COL4, 1:200, AB748, Chemicon), rabbit anti‐fibronectin (FN, 1:400, F3648, Sigma‐Aldrich), rabbit anti‐GJA1 (1:1000, C6219, Sigma), rabbit anti‐GJA1 (1:2000, ACC‐201, Alomone labs), rabbit anti‐GJB2 (1:100, ACC‐212, Alomone labs), rabbit anti‐GJB6 (1:100, PA511640, Thermo Scientific), rabbit anti‐GJE1 (1:1000, NBP2–14051, Novus biologicals), goat anti‐KCNJ10 (1:100, NBP1–70371), rabbit anti‐KCNQ1 (1:200, ab65092, Abcam), rabbit anti‐KCNQ1 (1:100, APC‐022, Alomone labs), rabbit anti‐KIT (1:100, A4502, Dako), rabbit anti‐laminin (LAM, 1:200, Z009701, Dako), rabbit anti‐melan‐A (1:500, NBP1–30151, Novus), mouse anti‐microphthalmia‐associated transcription factor (MITF, 1:100, M3621, Dako), mouse anti‐Na+ /K+ ‐ATPase α1 (ATP1A1, 1:200, α6F, Developmental Studies Hybridoma Bank), rabbit anti‐solute family carrier 2, member 1 (SLC2A1, 1:500, ab15309, Abcam), and goat anti‐SOX10 (1:50, sc‐17342, Santa Cruz Biotechnology).

    Techniques: Expressing