cx43  (Alomone Labs)


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    Alomone Labs cx43
    ( A ): Representative images depicting ethidium bromide (EtBr) uptake via hemichannels in hypothalamic (ARC) slices treated with either artificial cerebrospinal fluid (aCSF), 200 μM carbenoxolone (Cbx) or 100 µM the connexin-43 <t>(Cx43)</t> hemichannel blocker TAT-GAP19 for 15 min, then exposed to EtBr for 10 min. After fixation, slices were submitted to Glial Fibrillary Acidic Protein (GFAP) immunostaining and Hoechst labelling. Scale bar: 15 µm. ( B ): Quantification of EtBr fluorescence measured in GFAP+ astrocytes. n = 5, with each n representing the average of three replicates. ** p
    Cx43, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cx43/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cx43 - by Bioz Stars, 2022-08
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    Images

    1) Product Images from "Blockade of Glial Connexin 43 Hemichannels Reduces Food Intake"

    Article Title: Blockade of Glial Connexin 43 Hemichannels Reduces Food Intake

    Journal: Cells

    doi: 10.3390/cells9112387

    ( A ): Representative images depicting ethidium bromide (EtBr) uptake via hemichannels in hypothalamic (ARC) slices treated with either artificial cerebrospinal fluid (aCSF), 200 μM carbenoxolone (Cbx) or 100 µM the connexin-43 (Cx43) hemichannel blocker TAT-GAP19 for 15 min, then exposed to EtBr for 10 min. After fixation, slices were submitted to Glial Fibrillary Acidic Protein (GFAP) immunostaining and Hoechst labelling. Scale bar: 15 µm. ( B ): Quantification of EtBr fluorescence measured in GFAP+ astrocytes. n = 5, with each n representing the average of three replicates. ** p
    Figure Legend Snippet: ( A ): Representative images depicting ethidium bromide (EtBr) uptake via hemichannels in hypothalamic (ARC) slices treated with either artificial cerebrospinal fluid (aCSF), 200 μM carbenoxolone (Cbx) or 100 µM the connexin-43 (Cx43) hemichannel blocker TAT-GAP19 for 15 min, then exposed to EtBr for 10 min. After fixation, slices were submitted to Glial Fibrillary Acidic Protein (GFAP) immunostaining and Hoechst labelling. Scale bar: 15 µm. ( B ): Quantification of EtBr fluorescence measured in GFAP+ astrocytes. n = 5, with each n representing the average of three replicates. ** p

    Techniques Used: Immunostaining, Fluorescence

    ( A , B ): Connexin-43 (Cx43) and Glial Fibrillary Acidic Protein (GFAP) mRNA expression (normalized with Glyceraldehyde-3-phosphate dehydrogenase gene, GAPDH) was assessed by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) from the hypothalamus ( A ) and dorsal vagal complex (DVC) ( B ) of control ad libitum ( n = 14), 24 h fasted ( n = 13), 24 h fasted plus 2 h refeed ( n = 8) or plus 4 h refeed ( n = 8) mice. ( C ): Representative Western blot analysis of phosphorylated Cx43 (pS368) and Cx43 expression in the hypothalamus of control ad libitum ( n = 5) and 24 h fasted ( n = 5) mice. ( D , E ): Cx43 and GFAP mRNA expressions (normalized with GAPDH) were assessed by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) from the hypothalamus ( D ) and dorsal vagal complex (DVC) ( D ) of Normal Chow (NC) ( n = 9) and High Fat Diet (HFD) fed ( n = 9) mice. ( F ): Representative Western blot analysis and quantification of phosphorylated Cx43 (pS368) and Cx43 expression in the hypothalamus of NC ( n = 9) and HFD-fed ( n = 9) mice. A two-way analysis of variance test was performed between different experimental groups * p
    Figure Legend Snippet: ( A , B ): Connexin-43 (Cx43) and Glial Fibrillary Acidic Protein (GFAP) mRNA expression (normalized with Glyceraldehyde-3-phosphate dehydrogenase gene, GAPDH) was assessed by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) from the hypothalamus ( A ) and dorsal vagal complex (DVC) ( B ) of control ad libitum ( n = 14), 24 h fasted ( n = 13), 24 h fasted plus 2 h refeed ( n = 8) or plus 4 h refeed ( n = 8) mice. ( C ): Representative Western blot analysis of phosphorylated Cx43 (pS368) and Cx43 expression in the hypothalamus of control ad libitum ( n = 5) and 24 h fasted ( n = 5) mice. ( D , E ): Cx43 and GFAP mRNA expressions (normalized with GAPDH) were assessed by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) from the hypothalamus ( D ) and dorsal vagal complex (DVC) ( D ) of Normal Chow (NC) ( n = 9) and High Fat Diet (HFD) fed ( n = 9) mice. ( F ): Representative Western blot analysis and quantification of phosphorylated Cx43 (pS368) and Cx43 expression in the hypothalamus of NC ( n = 9) and HFD-fed ( n = 9) mice. A two-way analysis of variance test was performed between different experimental groups * p

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Mouse Assay, Western Blot

    ( A ): Confocal images of connexin-43 (Cx43) (red) and bassoon (green) double labeling immunohistochemistry performed within the nucleus tractus solitarius (NTS). Scale bar: 5 μm. Inset: Arrows show Cx43 labelling in close vicinity of synapses labelled by presynaptic bassoon protein. Scale bar 1 µm. ( B ): An example of a voxel (4 × 4 µm) used for the quantification of Cx43/bassoon appositions. Scale bar: 0.5 µm. ( C ): Twelve z-stack images defining the voxel illustrated in B and allowing the identification of Cx43/bassoon appositions. ( D ): Stereological estimation of Cx43/bassoon appositions in the NTS (85.13%) and arcuate nucleus of the hypothalamus (ARC) (80.46%) nuclei. ( E ): Table gathering the different stereological values acquired or estimated for each studied animal.
    Figure Legend Snippet: ( A ): Confocal images of connexin-43 (Cx43) (red) and bassoon (green) double labeling immunohistochemistry performed within the nucleus tractus solitarius (NTS). Scale bar: 5 μm. Inset: Arrows show Cx43 labelling in close vicinity of synapses labelled by presynaptic bassoon protein. Scale bar 1 µm. ( B ): An example of a voxel (4 × 4 µm) used for the quantification of Cx43/bassoon appositions. Scale bar: 0.5 µm. ( C ): Twelve z-stack images defining the voxel illustrated in B and allowing the identification of Cx43/bassoon appositions. ( D ): Stereological estimation of Cx43/bassoon appositions in the NTS (85.13%) and arcuate nucleus of the hypothalamus (ARC) (80.46%) nuclei. ( E ): Table gathering the different stereological values acquired or estimated for each studied animal.

    Techniques Used: Labeling, Immunohistochemistry

    ( A ): Cumulative food intake (g), measured over a 24 h period, of mice having received an i.c.v. injection of either vehicle (Sodium Chloride (NaCl) 0.9%) or TAT-GAP19 (12.5 or 25 µg). The TAT-GAP19-I14A, which includes a point mutation making it ineffective on connexin 43 hemichannels (Cx43 HCs) activity was tested on food intake at the dose of 25 µg. ( B ): Graph showing meal size over 24 h after i.c.v. injection of vehicle or TAT-GAP19 25 µg. Note that each bar represents a meal and its width the meal duration. The dark period is represented by the shaded box. ( C – F ): Meal microstructure analysis performed during the dark period for mice that received either vehicle or TAT-GAP19 25 µg allowed quantification of meals number ( C ), meals size (in mg of food, ( D )), meal duration (in min, ( E )) and interprandial period (in min, average intervals between meals, ( F – G )): Measure of energy expenditure (in Kcal/day-1/kg 0.75 ) within 12 h after injection of 0.9% NaCl vehicle ( n = 7) or 50 µg TAT-GAP19 ( n = 7). ( H , I ): Measure of total spontaneous activity (in Arbitrary Unit, UA; ( H ) and cumulative spontaneous activity ( I ) within 12 h for the 2 groups. ns: no significant difference; * p
    Figure Legend Snippet: ( A ): Cumulative food intake (g), measured over a 24 h period, of mice having received an i.c.v. injection of either vehicle (Sodium Chloride (NaCl) 0.9%) or TAT-GAP19 (12.5 or 25 µg). The TAT-GAP19-I14A, which includes a point mutation making it ineffective on connexin 43 hemichannels (Cx43 HCs) activity was tested on food intake at the dose of 25 µg. ( B ): Graph showing meal size over 24 h after i.c.v. injection of vehicle or TAT-GAP19 25 µg. Note that each bar represents a meal and its width the meal duration. The dark period is represented by the shaded box. ( C – F ): Meal microstructure analysis performed during the dark period for mice that received either vehicle or TAT-GAP19 25 µg allowed quantification of meals number ( C ), meals size (in mg of food, ( D )), meal duration (in min, ( E )) and interprandial period (in min, average intervals between meals, ( F – G )): Measure of energy expenditure (in Kcal/day-1/kg 0.75 ) within 12 h after injection of 0.9% NaCl vehicle ( n = 7) or 50 µg TAT-GAP19 ( n = 7). ( H , I ): Measure of total spontaneous activity (in Arbitrary Unit, UA; ( H ) and cumulative spontaneous activity ( I ) within 12 h for the 2 groups. ns: no significant difference; * p

    Techniques Used: Mouse Assay, Injection, Mutagenesis, Activity Assay

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    Alomone Labs anti connexin 43
    Diminished anterograde transport in a sample of DBA/2 J nerves.  a  Top Left: Coronal section through superior colliculus of DBA/2 J mouse (between dashed white lines) following intravitreal injection of CTB (green). Bottom Left: Corresponding retinotopic map shows nearly depleted anterograde transport of CTB. Top Right: Coronal section through superior colliculus of D2 control mouse prepared as on left. Bottom Right: Corresponding retinotopic map shows a full complement of anterogradely transported CTB.  b  Transport of CTB from DBA/2 J eyes was near 20% (22.8 ± 5.5), significantly reduced from D2 control which is near 75% (74.896 ± 4.328) ( p  = 0.0015).  c  Confocal micrographs of proximal (left) and distal (right) DBA/2 J (top) and D2 control (bottom) optic nerves. Connexin 43 (Cx43, blue) and GFAP (red) colocalize, and both are elevated in proximal optic nerve.  d . Density of Cx43 (puncta/μm 2 ) in proximal segment of DBA/2 J nerves is significantly elevated compared to both distal DBA/2 J (*;  p  = 0.043) and proximal D2 nerves (#;  p  = 0.032)
    Anti Connexin 43, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti connexin 43/product/Alomone Labs
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    Price from $9.99 to $1999.99
    anti connexin 43 - by Bioz Stars, 2022-08
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    Diminished anterograde transport in a sample of DBA/2 J nerves.  a  Top Left: Coronal section through superior colliculus of DBA/2 J mouse (between dashed white lines) following intravitreal injection of CTB (green). Bottom Left: Corresponding retinotopic map shows nearly depleted anterograde transport of CTB. Top Right: Coronal section through superior colliculus of D2 control mouse prepared as on left. Bottom Right: Corresponding retinotopic map shows a full complement of anterogradely transported CTB.  b  Transport of CTB from DBA/2 J eyes was near 20% (22.8 ± 5.5), significantly reduced from D2 control which is near 75% (74.896 ± 4.328) ( p  = 0.0015).  c  Confocal micrographs of proximal (left) and distal (right) DBA/2 J (top) and D2 control (bottom) optic nerves. Connexin 43 (Cx43, blue) and GFAP (red) colocalize, and both are elevated in proximal optic nerve.  d . Density of Cx43 (puncta/μm 2 ) in proximal segment of DBA/2 J nerves is significantly elevated compared to both distal DBA/2 J (*;  p  = 0.043) and proximal D2 nerves (#;  p  = 0.032)

    Journal: Acta Neuropathologica Communications

    Article Title: Astrocyte remodeling without gliosis precedes optic nerve Axonopathy

    doi: 10.1186/s40478-018-0542-0

    Figure Lengend Snippet: Diminished anterograde transport in a sample of DBA/2 J nerves. a Top Left: Coronal section through superior colliculus of DBA/2 J mouse (between dashed white lines) following intravitreal injection of CTB (green). Bottom Left: Corresponding retinotopic map shows nearly depleted anterograde transport of CTB. Top Right: Coronal section through superior colliculus of D2 control mouse prepared as on left. Bottom Right: Corresponding retinotopic map shows a full complement of anterogradely transported CTB. b Transport of CTB from DBA/2 J eyes was near 20% (22.8 ± 5.5), significantly reduced from D2 control which is near 75% (74.896 ± 4.328) ( p  = 0.0015). c Confocal micrographs of proximal (left) and distal (right) DBA/2 J (top) and D2 control (bottom) optic nerves. Connexin 43 (Cx43, blue) and GFAP (red) colocalize, and both are elevated in proximal optic nerve. d . Density of Cx43 (puncta/μm 2 ) in proximal segment of DBA/2 J nerves is significantly elevated compared to both distal DBA/2 J (*; p  = 0.043) and proximal D2 nerves (#; p  = 0.032)

    Article Snippet: We labeled 10 μm cryosections with the following antibodies: anti-glial fibrillary acidic protein (GFAP; EMD Millipore, Billerica, MA, 1:500), and anti-Connexin-43 (Cx43; Alomone Labs, Jerusalem, Israel, 1:250).

    Techniques: Injection, CtB Assay

    ( A ): Representative images depicting ethidium bromide (EtBr) uptake via hemichannels in hypothalamic (ARC) slices treated with either artificial cerebrospinal fluid (aCSF), 200 μM carbenoxolone (Cbx) or 100 µM the connexin-43 (Cx43) hemichannel blocker TAT-GAP19 for 15 min, then exposed to EtBr for 10 min. After fixation, slices were submitted to Glial Fibrillary Acidic Protein (GFAP) immunostaining and Hoechst labelling. Scale bar: 15 µm. ( B ): Quantification of EtBr fluorescence measured in GFAP+ astrocytes. n = 5, with each n representing the average of three replicates. ** p

    Journal: Cells

    Article Title: Blockade of Glial Connexin 43 Hemichannels Reduces Food Intake

    doi: 10.3390/cells9112387

    Figure Lengend Snippet: ( A ): Representative images depicting ethidium bromide (EtBr) uptake via hemichannels in hypothalamic (ARC) slices treated with either artificial cerebrospinal fluid (aCSF), 200 μM carbenoxolone (Cbx) or 100 µM the connexin-43 (Cx43) hemichannel blocker TAT-GAP19 for 15 min, then exposed to EtBr for 10 min. After fixation, slices were submitted to Glial Fibrillary Acidic Protein (GFAP) immunostaining and Hoechst labelling. Scale bar: 15 µm. ( B ): Quantification of EtBr fluorescence measured in GFAP+ astrocytes. n = 5, with each n representing the average of three replicates. ** p

    Article Snippet: Once again, the same saturation solution was used before the Cx43 rabbit primary antibody (#ACC-201 Alomone Labs 1/3000, Jerusalem, Israel) and goat anti-rabbit-IgG secondary antibody (1/400) on the same conditions.

    Techniques: Immunostaining, Fluorescence

    ( A , B ): Connexin-43 (Cx43) and Glial Fibrillary Acidic Protein (GFAP) mRNA expression (normalized with Glyceraldehyde-3-phosphate dehydrogenase gene, GAPDH) was assessed by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) from the hypothalamus ( A ) and dorsal vagal complex (DVC) ( B ) of control ad libitum ( n = 14), 24 h fasted ( n = 13), 24 h fasted plus 2 h refeed ( n = 8) or plus 4 h refeed ( n = 8) mice. ( C ): Representative Western blot analysis of phosphorylated Cx43 (pS368) and Cx43 expression in the hypothalamus of control ad libitum ( n = 5) and 24 h fasted ( n = 5) mice. ( D , E ): Cx43 and GFAP mRNA expressions (normalized with GAPDH) were assessed by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) from the hypothalamus ( D ) and dorsal vagal complex (DVC) ( D ) of Normal Chow (NC) ( n = 9) and High Fat Diet (HFD) fed ( n = 9) mice. ( F ): Representative Western blot analysis and quantification of phosphorylated Cx43 (pS368) and Cx43 expression in the hypothalamus of NC ( n = 9) and HFD-fed ( n = 9) mice. A two-way analysis of variance test was performed between different experimental groups * p

    Journal: Cells

    Article Title: Blockade of Glial Connexin 43 Hemichannels Reduces Food Intake

    doi: 10.3390/cells9112387

    Figure Lengend Snippet: ( A , B ): Connexin-43 (Cx43) and Glial Fibrillary Acidic Protein (GFAP) mRNA expression (normalized with Glyceraldehyde-3-phosphate dehydrogenase gene, GAPDH) was assessed by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) from the hypothalamus ( A ) and dorsal vagal complex (DVC) ( B ) of control ad libitum ( n = 14), 24 h fasted ( n = 13), 24 h fasted plus 2 h refeed ( n = 8) or plus 4 h refeed ( n = 8) mice. ( C ): Representative Western blot analysis of phosphorylated Cx43 (pS368) and Cx43 expression in the hypothalamus of control ad libitum ( n = 5) and 24 h fasted ( n = 5) mice. ( D , E ): Cx43 and GFAP mRNA expressions (normalized with GAPDH) were assessed by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) from the hypothalamus ( D ) and dorsal vagal complex (DVC) ( D ) of Normal Chow (NC) ( n = 9) and High Fat Diet (HFD) fed ( n = 9) mice. ( F ): Representative Western blot analysis and quantification of phosphorylated Cx43 (pS368) and Cx43 expression in the hypothalamus of NC ( n = 9) and HFD-fed ( n = 9) mice. A two-way analysis of variance test was performed between different experimental groups * p

    Article Snippet: Once again, the same saturation solution was used before the Cx43 rabbit primary antibody (#ACC-201 Alomone Labs 1/3000, Jerusalem, Israel) and goat anti-rabbit-IgG secondary antibody (1/400) on the same conditions.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Mouse Assay, Western Blot

    ( A ): Confocal images of connexin-43 (Cx43) (red) and bassoon (green) double labeling immunohistochemistry performed within the nucleus tractus solitarius (NTS). Scale bar: 5 μm. Inset: Arrows show Cx43 labelling in close vicinity of synapses labelled by presynaptic bassoon protein. Scale bar 1 µm. ( B ): An example of a voxel (4 × 4 µm) used for the quantification of Cx43/bassoon appositions. Scale bar: 0.5 µm. ( C ): Twelve z-stack images defining the voxel illustrated in B and allowing the identification of Cx43/bassoon appositions. ( D ): Stereological estimation of Cx43/bassoon appositions in the NTS (85.13%) and arcuate nucleus of the hypothalamus (ARC) (80.46%) nuclei. ( E ): Table gathering the different stereological values acquired or estimated for each studied animal.

    Journal: Cells

    Article Title: Blockade of Glial Connexin 43 Hemichannels Reduces Food Intake

    doi: 10.3390/cells9112387

    Figure Lengend Snippet: ( A ): Confocal images of connexin-43 (Cx43) (red) and bassoon (green) double labeling immunohistochemistry performed within the nucleus tractus solitarius (NTS). Scale bar: 5 μm. Inset: Arrows show Cx43 labelling in close vicinity of synapses labelled by presynaptic bassoon protein. Scale bar 1 µm. ( B ): An example of a voxel (4 × 4 µm) used for the quantification of Cx43/bassoon appositions. Scale bar: 0.5 µm. ( C ): Twelve z-stack images defining the voxel illustrated in B and allowing the identification of Cx43/bassoon appositions. ( D ): Stereological estimation of Cx43/bassoon appositions in the NTS (85.13%) and arcuate nucleus of the hypothalamus (ARC) (80.46%) nuclei. ( E ): Table gathering the different stereological values acquired or estimated for each studied animal.

    Article Snippet: Once again, the same saturation solution was used before the Cx43 rabbit primary antibody (#ACC-201 Alomone Labs 1/3000, Jerusalem, Israel) and goat anti-rabbit-IgG secondary antibody (1/400) on the same conditions.

    Techniques: Labeling, Immunohistochemistry

    ( A ): Cumulative food intake (g), measured over a 24 h period, of mice having received an i.c.v. injection of either vehicle (Sodium Chloride (NaCl) 0.9%) or TAT-GAP19 (12.5 or 25 µg). The TAT-GAP19-I14A, which includes a point mutation making it ineffective on connexin 43 hemichannels (Cx43 HCs) activity was tested on food intake at the dose of 25 µg. ( B ): Graph showing meal size over 24 h after i.c.v. injection of vehicle or TAT-GAP19 25 µg. Note that each bar represents a meal and its width the meal duration. The dark period is represented by the shaded box. ( C – F ): Meal microstructure analysis performed during the dark period for mice that received either vehicle or TAT-GAP19 25 µg allowed quantification of meals number ( C ), meals size (in mg of food, ( D )), meal duration (in min, ( E )) and interprandial period (in min, average intervals between meals, ( F – G )): Measure of energy expenditure (in Kcal/day-1/kg 0.75 ) within 12 h after injection of 0.9% NaCl vehicle ( n = 7) or 50 µg TAT-GAP19 ( n = 7). ( H , I ): Measure of total spontaneous activity (in Arbitrary Unit, UA; ( H ) and cumulative spontaneous activity ( I ) within 12 h for the 2 groups. ns: no significant difference; * p

    Journal: Cells

    Article Title: Blockade of Glial Connexin 43 Hemichannels Reduces Food Intake

    doi: 10.3390/cells9112387

    Figure Lengend Snippet: ( A ): Cumulative food intake (g), measured over a 24 h period, of mice having received an i.c.v. injection of either vehicle (Sodium Chloride (NaCl) 0.9%) or TAT-GAP19 (12.5 or 25 µg). The TAT-GAP19-I14A, which includes a point mutation making it ineffective on connexin 43 hemichannels (Cx43 HCs) activity was tested on food intake at the dose of 25 µg. ( B ): Graph showing meal size over 24 h after i.c.v. injection of vehicle or TAT-GAP19 25 µg. Note that each bar represents a meal and its width the meal duration. The dark period is represented by the shaded box. ( C – F ): Meal microstructure analysis performed during the dark period for mice that received either vehicle or TAT-GAP19 25 µg allowed quantification of meals number ( C ), meals size (in mg of food, ( D )), meal duration (in min, ( E )) and interprandial period (in min, average intervals between meals, ( F – G )): Measure of energy expenditure (in Kcal/day-1/kg 0.75 ) within 12 h after injection of 0.9% NaCl vehicle ( n = 7) or 50 µg TAT-GAP19 ( n = 7). ( H , I ): Measure of total spontaneous activity (in Arbitrary Unit, UA; ( H ) and cumulative spontaneous activity ( I ) within 12 h for the 2 groups. ns: no significant difference; * p

    Article Snippet: Once again, the same saturation solution was used before the Cx43 rabbit primary antibody (#ACC-201 Alomone Labs 1/3000, Jerusalem, Israel) and goat anti-rabbit-IgG secondary antibody (1/400) on the same conditions.

    Techniques: Mouse Assay, Injection, Mutagenesis, Activity Assay

    GJA1 is expressed by type I fibrocytes in the human fetal cochlea. (A) The lower basal turn of a cochlea at W14 immunostained for the gap junction protein GJA1 (green) and DAPI (blue). The dotted line outlines the group of spiral ligament fibrocytes expressing GJA1. The GJA1 signal is shown separately in white in (A′). Scale bar = 50 µm. (B) The lower basal turn of a cochlea at W18 immunostained for GJA1 (green) and DAPI (blue). The dotted line outlines the group of spiral ligament fibrocytes expressing GJA1. The GJA1 signal is shown separately in white in (B′). Scale bar = 50 µm. (C) Hematoxylin and eosin staining of a similar section as shown in (B). The dotted line represents the area of GJA1‐expressing fibrocytes in (B). Scale bar = 50 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com .]

    Journal: Developmental Neurobiology

    Article Title: Development of the stria vascularis and potassium regulation in the human fetal cochlea: Insights into hereditary sensorineural hearing loss

    doi: 10.1002/dneu.22279

    Figure Lengend Snippet: GJA1 is expressed by type I fibrocytes in the human fetal cochlea. (A) The lower basal turn of a cochlea at W14 immunostained for the gap junction protein GJA1 (green) and DAPI (blue). The dotted line outlines the group of spiral ligament fibrocytes expressing GJA1. The GJA1 signal is shown separately in white in (A′). Scale bar = 50 µm. (B) The lower basal turn of a cochlea at W18 immunostained for GJA1 (green) and DAPI (blue). The dotted line outlines the group of spiral ligament fibrocytes expressing GJA1. The GJA1 signal is shown separately in white in (B′). Scale bar = 50 µm. (C) Hematoxylin and eosin staining of a similar section as shown in (B). The dotted line represents the area of GJA1‐expressing fibrocytes in (B). Scale bar = 50 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com .]

    Article Snippet: The primary antibodies used in this study were mouse anti‐acetylated tubulin (aceTUBA, 1:500, T6793, Sigma), rabbit anti‐collagen type IV (COL4, 1:200, AB748, Chemicon), rabbit anti‐fibronectin (FN, 1:400, F3648, Sigma‐Aldrich), rabbit anti‐GJA1 (1:1000, C6219, Sigma), rabbit anti‐GJA1 (1:2000, ACC‐201, Alomone labs), rabbit anti‐GJB2 (1:100, ACC‐212, Alomone labs), rabbit anti‐GJB6 (1:100, PA511640, Thermo Scientific), rabbit anti‐GJE1 (1:1000, NBP2–14051, Novus biologicals), goat anti‐KCNJ10 (1:100, NBP1–70371), rabbit anti‐KCNQ1 (1:200, ab65092, Abcam), rabbit anti‐KCNQ1 (1:100, APC‐022, Alomone labs), rabbit anti‐KIT (1:100, A4502, Dako), rabbit anti‐laminin (LAM, 1:200, Z009701, Dako), rabbit anti‐melan‐A (1:500, NBP1–30151, Novus), mouse anti‐microphthalmia‐associated transcription factor (MITF, 1:100, M3621, Dako), mouse anti‐Na+ /K+ ‐ATPase α1 (ATP1A1, 1:200, α6F, Developmental Studies Hybridoma Bank), rabbit anti‐solute family carrier 2, member 1 (SLC2A1, 1:500, ab15309, Abcam), and goat anti‐SOX10 (1:50, sc‐17342, Santa Cruz Biotechnology).

    Techniques: Expressing, Staining

    Diminished anterograde transport in a sample of DBA/2 J nerves.  a  Top Left: Coronal section through superior colliculus of DBA/2 J mouse (between dashed white lines) following intravitreal injection of CTB (green). Bottom Left: Corresponding retinotopic map shows nearly depleted anterograde transport of CTB. Top Right: Coronal section through superior colliculus of D2 control mouse prepared as on left. Bottom Right: Corresponding retinotopic map shows a full complement of anterogradely transported CTB.  b  Transport of CTB from DBA/2 J eyes was near 20% (22.8 ± 5.5), significantly reduced from D2 control which is near 75% (74.896 ± 4.328) ( p  = 0.0015).  c  Confocal micrographs of proximal (left) and distal (right) DBA/2 J (top) and D2 control (bottom) optic nerves. Connexin 43 (Cx43, blue) and GFAP (red) colocalize, and both are elevated in proximal optic nerve.  d . Density of Cx43 (puncta/μm 2 ) in proximal segment of DBA/2 J nerves is significantly elevated compared to both distal DBA/2 J (*;  p  = 0.043) and proximal D2 nerves (#;  p  = 0.032)

    Journal: Acta Neuropathologica Communications

    Article Title: Astrocyte remodeling without gliosis precedes optic nerve Axonopathy

    doi: 10.1186/s40478-018-0542-0

    Figure Lengend Snippet: Diminished anterograde transport in a sample of DBA/2 J nerves. a Top Left: Coronal section through superior colliculus of DBA/2 J mouse (between dashed white lines) following intravitreal injection of CTB (green). Bottom Left: Corresponding retinotopic map shows nearly depleted anterograde transport of CTB. Top Right: Coronal section through superior colliculus of D2 control mouse prepared as on left. Bottom Right: Corresponding retinotopic map shows a full complement of anterogradely transported CTB. b Transport of CTB from DBA/2 J eyes was near 20% (22.8 ± 5.5), significantly reduced from D2 control which is near 75% (74.896 ± 4.328) ( p  = 0.0015). c Confocal micrographs of proximal (left) and distal (right) DBA/2 J (top) and D2 control (bottom) optic nerves. Connexin 43 (Cx43, blue) and GFAP (red) colocalize, and both are elevated in proximal optic nerve. d . Density of Cx43 (puncta/μm 2 ) in proximal segment of DBA/2 J nerves is significantly elevated compared to both distal DBA/2 J (*; p  = 0.043) and proximal D2 nerves (#; p  = 0.032)

    Article Snippet: We labeled 10 μm cryosections with the following antibodies: anti-glial fibrillary acidic protein (GFAP; EMD Millipore, Billerica, MA, 1:500), and anti-Connexin-43 (Cx43; Alomone Labs, Jerusalem, Israel, 1:250).

    Techniques: Injection, CtB Assay