trpc5  (Alomone Labs)


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    Structured Review

    Alomone Labs trpc5
    The <t>TRPC5</t> antibody occludes the effect of FFA in hcrt/orx neurons. (A) Transient (2 min) bath-application of 100 µM flufenamic acid (FFA), a non specific blocker of cationic currents, produces a strong membrane hyperpolarization and cessation of firing; inset: in voltage-clamp this hyperpolarization corresponds to an outward current. (B) Voltage-clamp ramps in absence (Ctrl) and presence (FFA) of flufenamic acid. The subtraction of voltage-clamp ramps shown in the inset suggests the presence of a voltage-dependent cationic current. (C–D) In hcrt/orx neurons loaded with a TRPC5 antibody, bath-application of FFA had only a small additional effect either in current-clamp (C) or in voltage-clamp (D).
    Trpc5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpc5/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpc5 - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Rat Hypocretin/Orexin Neurons Are Maintained in a Depolarized State by TRPC Channels"

    Article Title: Rat Hypocretin/Orexin Neurons Are Maintained in a Depolarized State by TRPC Channels

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0015673

    The TRPC5 antibody occludes the effect of FFA in hcrt/orx neurons. (A) Transient (2 min) bath-application of 100 µM flufenamic acid (FFA), a non specific blocker of cationic currents, produces a strong membrane hyperpolarization and cessation of firing; inset: in voltage-clamp this hyperpolarization corresponds to an outward current. (B) Voltage-clamp ramps in absence (Ctrl) and presence (FFA) of flufenamic acid. The subtraction of voltage-clamp ramps shown in the inset suggests the presence of a voltage-dependent cationic current. (C–D) In hcrt/orx neurons loaded with a TRPC5 antibody, bath-application of FFA had only a small additional effect either in current-clamp (C) or in voltage-clamp (D).
    Figure Legend Snippet: The TRPC5 antibody occludes the effect of FFA in hcrt/orx neurons. (A) Transient (2 min) bath-application of 100 µM flufenamic acid (FFA), a non specific blocker of cationic currents, produces a strong membrane hyperpolarization and cessation of firing; inset: in voltage-clamp this hyperpolarization corresponds to an outward current. (B) Voltage-clamp ramps in absence (Ctrl) and presence (FFA) of flufenamic acid. The subtraction of voltage-clamp ramps shown in the inset suggests the presence of a voltage-dependent cationic current. (C–D) In hcrt/orx neurons loaded with a TRPC5 antibody, bath-application of FFA had only a small additional effect either in current-clamp (C) or in voltage-clamp (D).

    Techniques Used:

    Dual immunohistochemical staining for TRPC5 and hcrt/orx. (A–B) Photomicrographs from a dual-immunostained section combining an hcrt/orx antibody from Santa Cruz (A) and a TRPC5 antibody from Alomone Labs (B). Examples of double labelled hcrt/orx-TRPC5 cells are indicated by white arrowheads and one such cell by a double circle (enlarged in the corresponding small panels to the right). Some hcrt/orx cells do not express TRPC5, as shown by an example indicated with a single circle. It is noteworthy that many hcrt/orx-negative cells also expressed TRPC5, as shown by an example indicated with a downward arrow (in panels A and B). (C–D) Same as in A and B with an hcrt/orx antibody from Phoenix Pharmaceuticals (C) and a TRPC5 antibody from NeuroMab (D). (Scale bars: 20 µm).
    Figure Legend Snippet: Dual immunohistochemical staining for TRPC5 and hcrt/orx. (A–B) Photomicrographs from a dual-immunostained section combining an hcrt/orx antibody from Santa Cruz (A) and a TRPC5 antibody from Alomone Labs (B). Examples of double labelled hcrt/orx-TRPC5 cells are indicated by white arrowheads and one such cell by a double circle (enlarged in the corresponding small panels to the right). Some hcrt/orx cells do not express TRPC5, as shown by an example indicated with a single circle. It is noteworthy that many hcrt/orx-negative cells also expressed TRPC5, as shown by an example indicated with a downward arrow (in panels A and B). (C–D) Same as in A and B with an hcrt/orx antibody from Phoenix Pharmaceuticals (C) and a TRPC5 antibody from NeuroMab (D). (Scale bars: 20 µm).

    Techniques Used: Immunohistochemistry, Staining

    The depolarized state of hcrt/orx neurons is blocked by a TRPC5 antibody. (A–C) In voltage-clamp configuration, intracellular application of either a TRPC1 (A) or a TRPC3 (B) or a TRPC4 (C) antibody (AbTRPC1, AbTRPC3 and AbTRPC4 respectively, all from Alomone Labs) has no effect on the current needed to clamp the cell at −50 mV. (D–E) Intracellular application of a TRPC5 antibody (Alomone Labs), in contrast, induces an outward current (D) that is blocked by co-application of a TRPC5 antigen (E). (F–G) In current-clamp configuration, intracellular application of a TRPC5 antibody alone (from either Alomone Labs (F) or NeuroMab (inset in F) induces an important membrane hyperpolarization and cessation of firing. In contrast, intracellular co-application of the TRPC5 antibody (Alomone Labs) together with its appropriate antigen (AgTRPC5) has no effect (G, to compare with F). (H) Voltage-clamp ramps in absence (Ctrl) and presence of a TRPC5 antibody (AbTRPC5, Alomone Labs). In the inset, subtraction of voltage-clamp ramps (Ctrl - AbTRPC5) suggests the presence of a voltage-dependent TRPC5 current.
    Figure Legend Snippet: The depolarized state of hcrt/orx neurons is blocked by a TRPC5 antibody. (A–C) In voltage-clamp configuration, intracellular application of either a TRPC1 (A) or a TRPC3 (B) or a TRPC4 (C) antibody (AbTRPC1, AbTRPC3 and AbTRPC4 respectively, all from Alomone Labs) has no effect on the current needed to clamp the cell at −50 mV. (D–E) Intracellular application of a TRPC5 antibody (Alomone Labs), in contrast, induces an outward current (D) that is blocked by co-application of a TRPC5 antigen (E). (F–G) In current-clamp configuration, intracellular application of a TRPC5 antibody alone (from either Alomone Labs (F) or NeuroMab (inset in F) induces an important membrane hyperpolarization and cessation of firing. In contrast, intracellular co-application of the TRPC5 antibody (Alomone Labs) together with its appropriate antigen (AgTRPC5) has no effect (G, to compare with F). (H) Voltage-clamp ramps in absence (Ctrl) and presence of a TRPC5 antibody (AbTRPC5, Alomone Labs). In the inset, subtraction of voltage-clamp ramps (Ctrl - AbTRPC5) suggests the presence of a voltage-dependent TRPC5 current.

    Techniques Used:

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    Alomone Labs trpc5
    The <t>TRPC5</t> antibody occludes the effect of FFA in hcrt/orx neurons. (A) Transient (2 min) bath-application of 100 µM flufenamic acid (FFA), a non specific blocker of cationic currents, produces a strong membrane hyperpolarization and cessation of firing; inset: in voltage-clamp this hyperpolarization corresponds to an outward current. (B) Voltage-clamp ramps in absence (Ctrl) and presence (FFA) of flufenamic acid. The subtraction of voltage-clamp ramps shown in the inset suggests the presence of a voltage-dependent cationic current. (C–D) In hcrt/orx neurons loaded with a TRPC5 antibody, bath-application of FFA had only a small additional effect either in current-clamp (C) or in voltage-clamp (D).
    Trpc5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpc5/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpc5 - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

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    The TRPC5 antibody occludes the effect of FFA in hcrt/orx neurons. (A) Transient (2 min) bath-application of 100 µM flufenamic acid (FFA), a non specific blocker of cationic currents, produces a strong membrane hyperpolarization and cessation of firing; inset: in voltage-clamp this hyperpolarization corresponds to an outward current. (B) Voltage-clamp ramps in absence (Ctrl) and presence (FFA) of flufenamic acid. The subtraction of voltage-clamp ramps shown in the inset suggests the presence of a voltage-dependent cationic current. (C–D) In hcrt/orx neurons loaded with a TRPC5 antibody, bath-application of FFA had only a small additional effect either in current-clamp (C) or in voltage-clamp (D).

    Journal: PLoS ONE

    Article Title: Rat Hypocretin/Orexin Neurons Are Maintained in a Depolarized State by TRPC Channels

    doi: 10.1371/journal.pone.0015673

    Figure Lengend Snippet: The TRPC5 antibody occludes the effect of FFA in hcrt/orx neurons. (A) Transient (2 min) bath-application of 100 µM flufenamic acid (FFA), a non specific blocker of cationic currents, produces a strong membrane hyperpolarization and cessation of firing; inset: in voltage-clamp this hyperpolarization corresponds to an outward current. (B) Voltage-clamp ramps in absence (Ctrl) and presence (FFA) of flufenamic acid. The subtraction of voltage-clamp ramps shown in the inset suggests the presence of a voltage-dependent cationic current. (C–D) In hcrt/orx neurons loaded with a TRPC5 antibody, bath-application of FFA had only a small additional effect either in current-clamp (C) or in voltage-clamp (D).

    Article Snippet: The primers used for TRPC1 (363 bp), TRPC5 (129 bp) and TRPC6 (114 bp) amplifications were from .

    Techniques:

    Dual immunohistochemical staining for TRPC5 and hcrt/orx. (A–B) Photomicrographs from a dual-immunostained section combining an hcrt/orx antibody from Santa Cruz (A) and a TRPC5 antibody from Alomone Labs (B). Examples of double labelled hcrt/orx-TRPC5 cells are indicated by white arrowheads and one such cell by a double circle (enlarged in the corresponding small panels to the right). Some hcrt/orx cells do not express TRPC5, as shown by an example indicated with a single circle. It is noteworthy that many hcrt/orx-negative cells also expressed TRPC5, as shown by an example indicated with a downward arrow (in panels A and B). (C–D) Same as in A and B with an hcrt/orx antibody from Phoenix Pharmaceuticals (C) and a TRPC5 antibody from NeuroMab (D). (Scale bars: 20 µm).

    Journal: PLoS ONE

    Article Title: Rat Hypocretin/Orexin Neurons Are Maintained in a Depolarized State by TRPC Channels

    doi: 10.1371/journal.pone.0015673

    Figure Lengend Snippet: Dual immunohistochemical staining for TRPC5 and hcrt/orx. (A–B) Photomicrographs from a dual-immunostained section combining an hcrt/orx antibody from Santa Cruz (A) and a TRPC5 antibody from Alomone Labs (B). Examples of double labelled hcrt/orx-TRPC5 cells are indicated by white arrowheads and one such cell by a double circle (enlarged in the corresponding small panels to the right). Some hcrt/orx cells do not express TRPC5, as shown by an example indicated with a single circle. It is noteworthy that many hcrt/orx-negative cells also expressed TRPC5, as shown by an example indicated with a downward arrow (in panels A and B). (C–D) Same as in A and B with an hcrt/orx antibody from Phoenix Pharmaceuticals (C) and a TRPC5 antibody from NeuroMab (D). (Scale bars: 20 µm).

    Article Snippet: The primers used for TRPC1 (363 bp), TRPC5 (129 bp) and TRPC6 (114 bp) amplifications were from .

    Techniques: Immunohistochemistry, Staining

    The depolarized state of hcrt/orx neurons is blocked by a TRPC5 antibody. (A–C) In voltage-clamp configuration, intracellular application of either a TRPC1 (A) or a TRPC3 (B) or a TRPC4 (C) antibody (AbTRPC1, AbTRPC3 and AbTRPC4 respectively, all from Alomone Labs) has no effect on the current needed to clamp the cell at −50 mV. (D–E) Intracellular application of a TRPC5 antibody (Alomone Labs), in contrast, induces an outward current (D) that is blocked by co-application of a TRPC5 antigen (E). (F–G) In current-clamp configuration, intracellular application of a TRPC5 antibody alone (from either Alomone Labs (F) or NeuroMab (inset in F) induces an important membrane hyperpolarization and cessation of firing. In contrast, intracellular co-application of the TRPC5 antibody (Alomone Labs) together with its appropriate antigen (AgTRPC5) has no effect (G, to compare with F). (H) Voltage-clamp ramps in absence (Ctrl) and presence of a TRPC5 antibody (AbTRPC5, Alomone Labs). In the inset, subtraction of voltage-clamp ramps (Ctrl - AbTRPC5) suggests the presence of a voltage-dependent TRPC5 current.

    Journal: PLoS ONE

    Article Title: Rat Hypocretin/Orexin Neurons Are Maintained in a Depolarized State by TRPC Channels

    doi: 10.1371/journal.pone.0015673

    Figure Lengend Snippet: The depolarized state of hcrt/orx neurons is blocked by a TRPC5 antibody. (A–C) In voltage-clamp configuration, intracellular application of either a TRPC1 (A) or a TRPC3 (B) or a TRPC4 (C) antibody (AbTRPC1, AbTRPC3 and AbTRPC4 respectively, all from Alomone Labs) has no effect on the current needed to clamp the cell at −50 mV. (D–E) Intracellular application of a TRPC5 antibody (Alomone Labs), in contrast, induces an outward current (D) that is blocked by co-application of a TRPC5 antigen (E). (F–G) In current-clamp configuration, intracellular application of a TRPC5 antibody alone (from either Alomone Labs (F) or NeuroMab (inset in F) induces an important membrane hyperpolarization and cessation of firing. In contrast, intracellular co-application of the TRPC5 antibody (Alomone Labs) together with its appropriate antigen (AgTRPC5) has no effect (G, to compare with F). (H) Voltage-clamp ramps in absence (Ctrl) and presence of a TRPC5 antibody (AbTRPC5, Alomone Labs). In the inset, subtraction of voltage-clamp ramps (Ctrl - AbTRPC5) suggests the presence of a voltage-dependent TRPC5 current.

    Article Snippet: The primers used for TRPC1 (363 bp), TRPC5 (129 bp) and TRPC6 (114 bp) amplifications were from .

    Techniques: