α Trpc4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons"
Article Title: Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons
Journal: Frontiers in Cellular Neuroscience
Figure Legend Snippet: SOC channel proteins in mouse cortical neurons. (A) Real time PCR (RT-PCR) of DNA complementary from primary cortical neurons obtained from E-15 wild-type mouse embryo with Orai 1–3, Stim 1, 2 and Trpc 1–7 specific primers. The experiment was repeated four times with similar results. (B) Western Blot analysis of ORAI 1, ORAI 2, STIM 2, TRPC 1 and TRPC 4 levels. Protein extracts were obtained from SH-SY5Y cells and 8–9 day in vitro (DIV) cortical neurons. Primary antibodies used were α-Orai1, α-Orai2, α-Trpc1, α-Trpc4, α-Stim2 and α -β Actin as a control.
Techniques Used: Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Western Blot, In Vitro