anti ip3 r 2  (Alomone Labs)


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    Alomone Labs anti ip3 r 2
    Localization of CCR5 in PC12 cell lines. PC12 cells differentiated for 48 h with db-cAMP were fixed and immunostained with <t>antibodies</t> against CCR5 receptor (green) and Na + /K + ATPase (plasma membrane marker, red). Nuclei were stained with Hoechst 33342 (blue). Representative confocal images are presented. Values shown in merged images represent average fluorescence intensity ± SEM (n = 5) of pixels positive in green channel (CCR5) that colocalize with red channel positive pixels (Na + /K + ATPase). ∗ P
    Anti Ip3 R 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ip3 r 2/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ip3 r 2 - by Bioz Stars, 2022-07
    92/100 stars

    Images

    1) Product Images from "Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells"

    Article Title: Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells

    Journal: BioMed Research International

    doi: 10.1155/2019/9616248

    Localization of CCR5 in PC12 cell lines. PC12 cells differentiated for 48 h with db-cAMP were fixed and immunostained with antibodies against CCR5 receptor (green) and Na + /K + ATPase (plasma membrane marker, red). Nuclei were stained with Hoechst 33342 (blue). Representative confocal images are presented. Values shown in merged images represent average fluorescence intensity ± SEM (n = 5) of pixels positive in green channel (CCR5) that colocalize with red channel positive pixels (Na + /K + ATPase). ∗ P
    Figure Legend Snippet: Localization of CCR5 in PC12 cell lines. PC12 cells differentiated for 48 h with db-cAMP were fixed and immunostained with antibodies against CCR5 receptor (green) and Na + /K + ATPase (plasma membrane marker, red). Nuclei were stained with Hoechst 33342 (blue). Representative confocal images are presented. Values shown in merged images represent average fluorescence intensity ± SEM (n = 5) of pixels positive in green channel (CCR5) that colocalize with red channel positive pixels (Na + /K + ATPase). ∗ P

    Techniques Used: Marker, Staining, Fluorescence

    Western blot analysis of IP 3 receptors in PC12 cell lines. (a) Approximately 60–80 μ g of total protein was resolved on an 8% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with anti-IP 3 R1, anti-IP 3 R2, anti-IP 3 R3, and recognizing all isoforms anti-IP 3 R-T antibodies. Representative blots are shown and arrows indicate the main band of the receptors. (b) Band intensity was densitometrically analyzed and the results are expressed as % (± SD) of control PC12 cells obtained after normalization to endogenous β -actin level. ∗ P
    Figure Legend Snippet: Western blot analysis of IP 3 receptors in PC12 cell lines. (a) Approximately 60–80 μ g of total protein was resolved on an 8% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with anti-IP 3 R1, anti-IP 3 R2, anti-IP 3 R3, and recognizing all isoforms anti-IP 3 R-T antibodies. Representative blots are shown and arrows indicate the main band of the receptors. (b) Band intensity was densitometrically analyzed and the results are expressed as % (± SD) of control PC12 cells obtained after normalization to endogenous β -actin level. ∗ P

    Techniques Used: Western Blot, SDS Page

    Western blot analysis of CCRs protein in PC12 cell lines. (a) Approximately 40–60 μ g of total protein was resolved on a 10% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with polyclonal anti-CCR1, polyclonal anti-CCR3, or monoclonal anti-CCR5. Representative blots are shown and arrows indicate the main band of the receptors with apparent MW 41 kDa for CCR1, 40 kDa for CCR3, and 46 kDa for CCR5. (b) The bands intensity was densitometrically analyzed and the results are expressed as % of the control PC12 cells obtained after normalization to endogenous β -actin level (± SD). ∗ P
    Figure Legend Snippet: Western blot analysis of CCRs protein in PC12 cell lines. (a) Approximately 40–60 μ g of total protein was resolved on a 10% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with polyclonal anti-CCR1, polyclonal anti-CCR3, or monoclonal anti-CCR5. Representative blots are shown and arrows indicate the main band of the receptors with apparent MW 41 kDa for CCR1, 40 kDa for CCR3, and 46 kDa for CCR5. (b) The bands intensity was densitometrically analyzed and the results are expressed as % of the control PC12 cells obtained after normalization to endogenous β -actin level (± SD). ∗ P

    Techniques Used: Western Blot, SDS Page

    2) Product Images from "Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells"

    Article Title: Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells

    Journal: BioMed Research International

    doi: 10.1155/2019/9616248

    Localization of CCR5 in PC12 cell lines. PC12 cells differentiated for 48 h with db-cAMP were fixed and immunostained with antibodies against CCR5 receptor (green) and Na + /K + ATPase (plasma membrane marker, red). Nuclei were stained with Hoechst 33342 (blue). Representative confocal images are presented. Values shown in merged images represent average fluorescence intensity ± SEM (n = 5) of pixels positive in green channel (CCR5) that colocalize with red channel positive pixels (Na + /K + ATPase). ∗ P
    Figure Legend Snippet: Localization of CCR5 in PC12 cell lines. PC12 cells differentiated for 48 h with db-cAMP were fixed and immunostained with antibodies against CCR5 receptor (green) and Na + /K + ATPase (plasma membrane marker, red). Nuclei were stained with Hoechst 33342 (blue). Representative confocal images are presented. Values shown in merged images represent average fluorescence intensity ± SEM (n = 5) of pixels positive in green channel (CCR5) that colocalize with red channel positive pixels (Na + /K + ATPase). ∗ P

    Techniques Used: Marker, Staining, Fluorescence

    Western blot analysis of IP 3 receptors in PC12 cell lines. (a) Approximately 60–80 μ g of total protein was resolved on an 8% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with anti-IP 3 R1, anti-IP 3 R2, anti-IP 3 R3, and recognizing all isoforms anti-IP 3 R-T antibodies. Representative blots are shown and arrows indicate the main band of the receptors. (b) Band intensity was densitometrically analyzed and the results are expressed as % (± SD) of control PC12 cells obtained after normalization to endogenous β -actin level. ∗ P
    Figure Legend Snippet: Western blot analysis of IP 3 receptors in PC12 cell lines. (a) Approximately 60–80 μ g of total protein was resolved on an 8% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with anti-IP 3 R1, anti-IP 3 R2, anti-IP 3 R3, and recognizing all isoforms anti-IP 3 R-T antibodies. Representative blots are shown and arrows indicate the main band of the receptors. (b) Band intensity was densitometrically analyzed and the results are expressed as % (± SD) of control PC12 cells obtained after normalization to endogenous β -actin level. ∗ P

    Techniques Used: Western Blot, SDS Page

    Western blot analysis of CCRs protein in PC12 cell lines. (a) Approximately 40–60 μ g of total protein was resolved on a 10% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with polyclonal anti-CCR1, polyclonal anti-CCR3, or monoclonal anti-CCR5. Representative blots are shown and arrows indicate the main band of the receptors with apparent MW 41 kDa for CCR1, 40 kDa for CCR3, and 46 kDa for CCR5. (b) The bands intensity was densitometrically analyzed and the results are expressed as % of the control PC12 cells obtained after normalization to endogenous β -actin level (± SD). ∗ P
    Figure Legend Snippet: Western blot analysis of CCRs protein in PC12 cell lines. (a) Approximately 40–60 μ g of total protein was resolved on a 10% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with polyclonal anti-CCR1, polyclonal anti-CCR3, or monoclonal anti-CCR5. Representative blots are shown and arrows indicate the main band of the receptors with apparent MW 41 kDa for CCR1, 40 kDa for CCR3, and 46 kDa for CCR5. (b) The bands intensity was densitometrically analyzed and the results are expressed as % of the control PC12 cells obtained after normalization to endogenous β -actin level (± SD). ∗ P

    Techniques Used: Western Blot, SDS Page

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    Alomone Labs anti ip3 r 2
    Localization of CCR5 in PC12 cell lines. PC12 cells differentiated for 48 h with db-cAMP were fixed and immunostained with <t>antibodies</t> against CCR5 receptor (green) and Na + /K + ATPase (plasma membrane marker, red). Nuclei were stained with Hoechst 33342 (blue). Representative confocal images are presented. Values shown in merged images represent average fluorescence intensity ± SEM (n = 5) of pixels positive in green channel (CCR5) that colocalize with red channel positive pixels (Na + /K + ATPase). ∗ P
    Anti Ip3 R 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ip3 r 2/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ip3 r 2 - by Bioz Stars, 2022-07
    92/100 stars
      Buy from Supplier

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    Localization of CCR5 in PC12 cell lines. PC12 cells differentiated for 48 h with db-cAMP were fixed and immunostained with antibodies against CCR5 receptor (green) and Na + /K + ATPase (plasma membrane marker, red). Nuclei were stained with Hoechst 33342 (blue). Representative confocal images are presented. Values shown in merged images represent average fluorescence intensity ± SEM (n = 5) of pixels positive in green channel (CCR5) that colocalize with red channel positive pixels (Na + /K + ATPase). ∗ P

    Journal: BioMed Research International

    Article Title: Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells

    doi: 10.1155/2019/9616248

    Figure Lengend Snippet: Localization of CCR5 in PC12 cell lines. PC12 cells differentiated for 48 h with db-cAMP were fixed and immunostained with antibodies against CCR5 receptor (green) and Na + /K + ATPase (plasma membrane marker, red). Nuclei were stained with Hoechst 33342 (blue). Representative confocal images are presented. Values shown in merged images represent average fluorescence intensity ± SEM (n = 5) of pixels positive in green channel (CCR5) that colocalize with red channel positive pixels (Na + /K + ATPase). ∗ P

    Article Snippet: The following primary antibodies were used: anti-CCR1 (rabbit, polyclonal, 1:1000, Santa Cruz Biotechnology; rabbit, polyclonal, 1:1000, BosterBio), anti-CCR3 (rabbit, polyclonal, 1:1000, Santa Cruz Biotechnology; rabbit, monoclonal, 1:1000, BosterBio), anti-CCR5 (mouse, monoclonal, 1:1000, Santa Cruz Biotechnology), anti-IP3 R-1 (rabbit, polyclonal, 1:2000, Alomone Labs), anti-IP3 R-2 (rabbit, polyclonal, 1:200, Alomone Labs), monoclonal anti-IP3 R-3 (mouse, monoclonal, 1:2000, BD Biosciences), and anti-IP3 R-1/2/3 (mouse, monoclonal, 1:500, Santa Cruz Biotechnology).

    Techniques: Marker, Staining, Fluorescence

    Western blot analysis of IP 3 receptors in PC12 cell lines. (a) Approximately 60–80 μ g of total protein was resolved on an 8% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with anti-IP 3 R1, anti-IP 3 R2, anti-IP 3 R3, and recognizing all isoforms anti-IP 3 R-T antibodies. Representative blots are shown and arrows indicate the main band of the receptors. (b) Band intensity was densitometrically analyzed and the results are expressed as % (± SD) of control PC12 cells obtained after normalization to endogenous β -actin level. ∗ P

    Journal: BioMed Research International

    Article Title: Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells

    doi: 10.1155/2019/9616248

    Figure Lengend Snippet: Western blot analysis of IP 3 receptors in PC12 cell lines. (a) Approximately 60–80 μ g of total protein was resolved on an 8% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with anti-IP 3 R1, anti-IP 3 R2, anti-IP 3 R3, and recognizing all isoforms anti-IP 3 R-T antibodies. Representative blots are shown and arrows indicate the main band of the receptors. (b) Band intensity was densitometrically analyzed and the results are expressed as % (± SD) of control PC12 cells obtained after normalization to endogenous β -actin level. ∗ P

    Article Snippet: The following primary antibodies were used: anti-CCR1 (rabbit, polyclonal, 1:1000, Santa Cruz Biotechnology; rabbit, polyclonal, 1:1000, BosterBio), anti-CCR3 (rabbit, polyclonal, 1:1000, Santa Cruz Biotechnology; rabbit, monoclonal, 1:1000, BosterBio), anti-CCR5 (mouse, monoclonal, 1:1000, Santa Cruz Biotechnology), anti-IP3 R-1 (rabbit, polyclonal, 1:2000, Alomone Labs), anti-IP3 R-2 (rabbit, polyclonal, 1:200, Alomone Labs), monoclonal anti-IP3 R-3 (mouse, monoclonal, 1:2000, BD Biosciences), and anti-IP3 R-1/2/3 (mouse, monoclonal, 1:500, Santa Cruz Biotechnology).

    Techniques: Western Blot, SDS Page

    Western blot analysis of CCRs protein in PC12 cell lines. (a) Approximately 40–60 μ g of total protein was resolved on a 10% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with polyclonal anti-CCR1, polyclonal anti-CCR3, or monoclonal anti-CCR5. Representative blots are shown and arrows indicate the main band of the receptors with apparent MW 41 kDa for CCR1, 40 kDa for CCR3, and 46 kDa for CCR5. (b) The bands intensity was densitometrically analyzed and the results are expressed as % of the control PC12 cells obtained after normalization to endogenous β -actin level (± SD). ∗ P

    Journal: BioMed Research International

    Article Title: Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells

    doi: 10.1155/2019/9616248

    Figure Lengend Snippet: Western blot analysis of CCRs protein in PC12 cell lines. (a) Approximately 40–60 μ g of total protein was resolved on a 10% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with polyclonal anti-CCR1, polyclonal anti-CCR3, or monoclonal anti-CCR5. Representative blots are shown and arrows indicate the main band of the receptors with apparent MW 41 kDa for CCR1, 40 kDa for CCR3, and 46 kDa for CCR5. (b) The bands intensity was densitometrically analyzed and the results are expressed as % of the control PC12 cells obtained after normalization to endogenous β -actin level (± SD). ∗ P

    Article Snippet: The following primary antibodies were used: anti-CCR1 (rabbit, polyclonal, 1:1000, Santa Cruz Biotechnology; rabbit, polyclonal, 1:1000, BosterBio), anti-CCR3 (rabbit, polyclonal, 1:1000, Santa Cruz Biotechnology; rabbit, monoclonal, 1:1000, BosterBio), anti-CCR5 (mouse, monoclonal, 1:1000, Santa Cruz Biotechnology), anti-IP3 R-1 (rabbit, polyclonal, 1:2000, Alomone Labs), anti-IP3 R-2 (rabbit, polyclonal, 1:200, Alomone Labs), monoclonal anti-IP3 R-3 (mouse, monoclonal, 1:2000, BD Biosciences), and anti-IP3 R-1/2/3 (mouse, monoclonal, 1:500, Santa Cruz Biotechnology).

    Techniques: Western Blot, SDS Page