Rabbit Polyclonal Anti Neuregulin 1 Nrg1 Type Iii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "The Y172 Monoclonal Antibody Against p-c-Jun (Ser63) Is a Marker of the Postsynaptic Compartment of C-Type Cholinergic Afferent Synapses on Motoneurons"
Article Title: The Y172 Monoclonal Antibody Against p-c-Jun (Ser63) Is a Marker of the Postsynaptic Compartment of C-Type Cholinergic Afferent Synapses on Motoneurons
Journal: Frontiers in Cellular Neuroscience
Figure Legend Snippet: Changes in Y172 immunoreactivity in MNs from mutant mice (P60) overexpressing NRG1 type III. (A–F) The density (per 100 μm 2 MN soma, A,B ) and size (in μm 2 , C,D ) of total (A,C) and peripheral (periph., B,D ) Y172-positive profiles and the percentage of these profiles showing a spatial association with VAChT-positive C-boutons (E) and NRG1 type III-positive spots (F) in MNs from WT and NRG1 type III-overexpressing mice. Note that NRG1 type III overexpression was associated with a prominent decrease in the density of total Y172-positive profiles (A) and a significant increase in the number of those located peripherally (B) in MNs; the area of both total and peripheral Y172-positive profiles was dramatically increased in MNs from NRG1 type III-overexpressing animals (C,D) . Additionally, the percentage of Y172-positive profiles showing a close association with VAChT- (E) or NRG1 type III-positive (F) spots significantly increased or decreased, respectively, in MNs from NRG1 type III-overexpressing animals; 10–15 randomly selected MNs from 3 to 4 mice per condition were analyzed; * p
Techniques Used: Mutagenesis, Mouse Assay, Over Expression
Figure Legend Snippet: Y172 immunoreactivity colocalizes with S1R and Kv2.1 in C-boutons. (A1–D4) Representative confocal images of adult (P75) mouse spinal cord MNs double immunostained with antibodies against either S1R or Kv2.1 (red) and Y172 (green), as indicated in the panels; sections were counterstained with fluorescent Nissl stain (blue) for MN visualization. The areas delimited by dotted-line squares in (A4,C4) are shown at higher magnification in (B1–B4,D1–D4) , respectively. (E–H) Pixel profile analysis (F,H ) along the lines depicted in (E,G) , which were obtained from MNs immunolabeled with Y172 (green) and anti-S1R or Kv2.1 (red) antibodies; fluorescent Nissl staining (blue) for MN visualization can be seen in (E) . Note the overlap between the Y172 and S1R (F) or Kv2.1 (H) signals. (I,J) Squashed MNs immunolabeled with anti-NRG1 (I) and Y172 (J) antibodies; the circled profiles are shown at higher magnification in the insets; note that Y172-positive profiles were smaller and displayed more compact patterns than NRG1 clusters. Scale bars: A4,C4 = 10 μm (valid for A1–A3,C1–C3 ); B4,D4 = 1 μm (valid for B1–B3,D1–D3 ); G = 1 μm (valid for E ); and J = 20 μm (valid for I ).
Techniques Used: Staining, Immunolabeling