primary antibodies against k ca 3 1  (Alomone Labs)


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    Alomone Labs primary antibodies against k ca 3 1
    Functional expression of K Ca 3.1 in THP-1-derived M 2 macrophages. ( A , B ): Simultaneous measurements of changes in the membrane potential ( A ) and [Ca 2+ ] i ( B ) in the three different cells [a (black), b (blue), and c (red)], following the application of a selective K Ca 3.1 activator, SKA-121 (1 μM), and/or the K Ca 3.1 inhibitor, TRAM-34 (1 μM) using DiBAC 4 (3) and Fura 2, respectively. The relative time-course changes in fluorescence intensities (1.0 at 0 s) from three different THP-1-derived M 2 macrophages are shown. ( C ): Real-time PCR examination of K Ca 3.1 expression in THP-1-derived M 0 macrophages (‘M 0 ’) and M 2 macrophages (‘M 2 ’). Expression levels are shown as a ratio to ACTB ( p > 0.05, n = 4 for each). ( D , E ): Protein expression levels of K Ca 3.1 in the ‘M 0 ’ and ‘M 2 ’ groups were determined by Western blot. Specific band signals for K Ca 3.1 were observed at approximately 50 kDa ( D , upper panel). After compensation with the optical density of the ACTB signal (43 kDa) ( D , lower panel), the expression level in the ‘M 0 ’ group was expressed as 1.0 ( p > 0.05, n = 4 for each) ( E ). ( F ): SKA-121 (1 μM)-induced relative hyperpolarizing responses in the ‘M 0 ’ and ‘M 2 ’ groups ( p > 0.05, n = 37 and 29, respectively).
    Primary Antibodies Against K Ca 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Downregulation of IL-8 and IL-10 by the Activation of Ca 2+ -Activated K + Channel K Ca 3.1 in THP-1-Derived M 2 Macrophages"

    Article Title: Downregulation of IL-8 and IL-10 by the Activation of Ca 2+ -Activated K + Channel K Ca 3.1 in THP-1-Derived M 2 Macrophages

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23158603

    Functional expression of K Ca 3.1 in THP-1-derived M 2 macrophages. ( A , B ): Simultaneous measurements of changes in the membrane potential ( A ) and [Ca 2+ ] i ( B ) in the three different cells [a (black), b (blue), and c (red)], following the application of a selective K Ca 3.1 activator, SKA-121 (1 μM), and/or the K Ca 3.1 inhibitor, TRAM-34 (1 μM) using DiBAC 4 (3) and Fura 2, respectively. The relative time-course changes in fluorescence intensities (1.0 at 0 s) from three different THP-1-derived M 2 macrophages are shown. ( C ): Real-time PCR examination of K Ca 3.1 expression in THP-1-derived M 0 macrophages (‘M 0 ’) and M 2 macrophages (‘M 2 ’). Expression levels are shown as a ratio to ACTB ( p > 0.05, n = 4 for each). ( D , E ): Protein expression levels of K Ca 3.1 in the ‘M 0 ’ and ‘M 2 ’ groups were determined by Western blot. Specific band signals for K Ca 3.1 were observed at approximately 50 kDa ( D , upper panel). After compensation with the optical density of the ACTB signal (43 kDa) ( D , lower panel), the expression level in the ‘M 0 ’ group was expressed as 1.0 ( p > 0.05, n = 4 for each) ( E ). ( F ): SKA-121 (1 μM)-induced relative hyperpolarizing responses in the ‘M 0 ’ and ‘M 2 ’ groups ( p > 0.05, n = 37 and 29, respectively).
    Figure Legend Snippet: Functional expression of K Ca 3.1 in THP-1-derived M 2 macrophages. ( A , B ): Simultaneous measurements of changes in the membrane potential ( A ) and [Ca 2+ ] i ( B ) in the three different cells [a (black), b (blue), and c (red)], following the application of a selective K Ca 3.1 activator, SKA-121 (1 μM), and/or the K Ca 3.1 inhibitor, TRAM-34 (1 μM) using DiBAC 4 (3) and Fura 2, respectively. The relative time-course changes in fluorescence intensities (1.0 at 0 s) from three different THP-1-derived M 2 macrophages are shown. ( C ): Real-time PCR examination of K Ca 3.1 expression in THP-1-derived M 0 macrophages (‘M 0 ’) and M 2 macrophages (‘M 2 ’). Expression levels are shown as a ratio to ACTB ( p > 0.05, n = 4 for each). ( D , E ): Protein expression levels of K Ca 3.1 in the ‘M 0 ’ and ‘M 2 ’ groups were determined by Western blot. Specific band signals for K Ca 3.1 were observed at approximately 50 kDa ( D , upper panel). After compensation with the optical density of the ACTB signal (43 kDa) ( D , lower panel), the expression level in the ‘M 0 ’ group was expressed as 1.0 ( p > 0.05, n = 4 for each) ( E ). ( F ): SKA-121 (1 μM)-induced relative hyperpolarizing responses in the ‘M 0 ’ and ‘M 2 ’ groups ( p > 0.05, n = 37 and 29, respectively).

    Techniques Used: Functional Assay, Expressing, Derivative Assay, Fluorescence, Real-time Polymerase Chain Reaction, Western Blot

    110 cs gluconate  (Alomone Labs)


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    Alomone Labs 110 cs gluconate
    110 Cs Gluconate, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    primary antibodies against k ca 3 1  (Alomone Labs)


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    Alomone Labs primary antibodies against k ca 3 1
    Functional expression of K Ca 3.1 in THP-1-derived M 2 macrophages. ( A , B ): Simultaneous measurements of changes in the membrane potential ( A ) and [Ca 2+ ] i ( B ) in the three different cells [a (black), b (blue), and c (red)], following the application of a selective K Ca 3.1 activator, SKA-121 (1 μM), and/or the K Ca 3.1 inhibitor, TRAM-34 (1 μM) using DiBAC 4 (3) and Fura 2, respectively. The relative time-course changes in fluorescence intensities (1.0 at 0 s) from three different THP-1-derived M 2 macrophages are shown. ( C ): Real-time PCR examination of K Ca 3.1 expression in THP-1-derived M 0 macrophages (‘M 0 ’) and M 2 macrophages (‘M 2 ’). Expression levels are shown as a ratio to ACTB ( p > 0.05, n = 4 for each). ( D , E ): Protein expression levels of K Ca 3.1 in the ‘M 0 ’ and ‘M 2 ’ groups were determined by Western blot. Specific band signals for K Ca 3.1 were observed at approximately 50 kDa ( D , upper panel). After compensation with the optical density of the ACTB signal (43 kDa) ( D , lower panel), the expression level in the ‘M 0 ’ group was expressed as 1.0 ( p > 0.05, n = 4 for each) ( E ). ( F ): SKA-121 (1 μM)-induced relative hyperpolarizing responses in the ‘M 0 ’ and ‘M 2 ’ groups ( p > 0.05, n = 37 and 29, respectively).
    Primary Antibodies Against K Ca 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    94/100 stars

    Images

    1) Product Images from "Downregulation of IL-8 and IL-10 by the Activation of Ca 2+ -Activated K + Channel K Ca 3.1 in THP-1-Derived M 2 Macrophages"

    Article Title: Downregulation of IL-8 and IL-10 by the Activation of Ca 2+ -Activated K + Channel K Ca 3.1 in THP-1-Derived M 2 Macrophages

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23158603

    Functional expression of K Ca 3.1 in THP-1-derived M 2 macrophages. ( A , B ): Simultaneous measurements of changes in the membrane potential ( A ) and [Ca 2+ ] i ( B ) in the three different cells [a (black), b (blue), and c (red)], following the application of a selective K Ca 3.1 activator, SKA-121 (1 μM), and/or the K Ca 3.1 inhibitor, TRAM-34 (1 μM) using DiBAC 4 (3) and Fura 2, respectively. The relative time-course changes in fluorescence intensities (1.0 at 0 s) from three different THP-1-derived M 2 macrophages are shown. ( C ): Real-time PCR examination of K Ca 3.1 expression in THP-1-derived M 0 macrophages (‘M 0 ’) and M 2 macrophages (‘M 2 ’). Expression levels are shown as a ratio to ACTB ( p > 0.05, n = 4 for each). ( D , E ): Protein expression levels of K Ca 3.1 in the ‘M 0 ’ and ‘M 2 ’ groups were determined by Western blot. Specific band signals for K Ca 3.1 were observed at approximately 50 kDa ( D , upper panel). After compensation with the optical density of the ACTB signal (43 kDa) ( D , lower panel), the expression level in the ‘M 0 ’ group was expressed as 1.0 ( p > 0.05, n = 4 for each) ( E ). ( F ): SKA-121 (1 μM)-induced relative hyperpolarizing responses in the ‘M 0 ’ and ‘M 2 ’ groups ( p > 0.05, n = 37 and 29, respectively).
    Figure Legend Snippet: Functional expression of K Ca 3.1 in THP-1-derived M 2 macrophages. ( A , B ): Simultaneous measurements of changes in the membrane potential ( A ) and [Ca 2+ ] i ( B ) in the three different cells [a (black), b (blue), and c (red)], following the application of a selective K Ca 3.1 activator, SKA-121 (1 μM), and/or the K Ca 3.1 inhibitor, TRAM-34 (1 μM) using DiBAC 4 (3) and Fura 2, respectively. The relative time-course changes in fluorescence intensities (1.0 at 0 s) from three different THP-1-derived M 2 macrophages are shown. ( C ): Real-time PCR examination of K Ca 3.1 expression in THP-1-derived M 0 macrophages (‘M 0 ’) and M 2 macrophages (‘M 2 ’). Expression levels are shown as a ratio to ACTB ( p > 0.05, n = 4 for each). ( D , E ): Protein expression levels of K Ca 3.1 in the ‘M 0 ’ and ‘M 2 ’ groups were determined by Western blot. Specific band signals for K Ca 3.1 were observed at approximately 50 kDa ( D , upper panel). After compensation with the optical density of the ACTB signal (43 kDa) ( D , lower panel), the expression level in the ‘M 0 ’ group was expressed as 1.0 ( p > 0.05, n = 4 for each) ( E ). ( F ): SKA-121 (1 μM)-induced relative hyperpolarizing responses in the ‘M 0 ’ and ‘M 2 ’ groups ( p > 0.05, n = 37 and 29, respectively).

    Techniques Used: Functional Assay, Expressing, Derivative Assay, Fluorescence, Real-time Polymerase Chain Reaction, Western Blot

    rabbit polyclonal ca v 1 3  (Alomone Labs)


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    Alomone Labs rabbit polyclonal ca v 1 3
    (A) Representative western blots for Ca V 1.2 and Ca V 1.3 channels and total protein stains from pacemaker tissue explants from young and old mice. (B) Fold change of Ca V 1.2 and Ca V 1.3 channel total expression in old animals relative to young. Expression was normalized to total protein and each data point represents an animal. Statistical comparisons used a two-tail Mann-Whitney test. (C-F) Small insets to the right are representative AiryScan high-resolution images of the footprint of young and old pacemaker cells labeled against Ca V 1.2 (magenta) or Ca V 1.3 (orange). Magnified panels to the left are 5 by 5 µm footprint regions from each of the cells shown to the right. (G) Comparison of Ca V 1.2 and Ca V 1.3 particle density between young (Ca V 1.2, n = 22, N = 3; Ca V 1.3, n = 16, N = 3) and old (Ca V 1.2, n= 33, N = 3; Ca V 1.3, n = 23, N = 3) cells. Statistical comparisons used a two-tail t-test.
    Rabbit Polyclonal Ca V 1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Aging impairs the clustering and reduces the activity of L-type calcium channels in cardiac pacemaker cells"

    Article Title: Aging impairs the clustering and reduces the activity of L-type calcium channels in cardiac pacemaker cells

    Journal: bioRxiv

    doi: 10.1101/2022.06.22.497267

    (A) Representative western blots for Ca V 1.2 and Ca V 1.3 channels and total protein stains from pacemaker tissue explants from young and old mice. (B) Fold change of Ca V 1.2 and Ca V 1.3 channel total expression in old animals relative to young. Expression was normalized to total protein and each data point represents an animal. Statistical comparisons used a two-tail Mann-Whitney test. (C-F) Small insets to the right are representative AiryScan high-resolution images of the footprint of young and old pacemaker cells labeled against Ca V 1.2 (magenta) or Ca V 1.3 (orange). Magnified panels to the left are 5 by 5 µm footprint regions from each of the cells shown to the right. (G) Comparison of Ca V 1.2 and Ca V 1.3 particle density between young (Ca V 1.2, n = 22, N = 3; Ca V 1.3, n = 16, N = 3) and old (Ca V 1.2, n= 33, N = 3; Ca V 1.3, n = 23, N = 3) cells. Statistical comparisons used a two-tail t-test.
    Figure Legend Snippet: (A) Representative western blots for Ca V 1.2 and Ca V 1.3 channels and total protein stains from pacemaker tissue explants from young and old mice. (B) Fold change of Ca V 1.2 and Ca V 1.3 channel total expression in old animals relative to young. Expression was normalized to total protein and each data point represents an animal. Statistical comparisons used a two-tail Mann-Whitney test. (C-F) Small insets to the right are representative AiryScan high-resolution images of the footprint of young and old pacemaker cells labeled against Ca V 1.2 (magenta) or Ca V 1.3 (orange). Magnified panels to the left are 5 by 5 µm footprint regions from each of the cells shown to the right. (G) Comparison of Ca V 1.2 and Ca V 1.3 particle density between young (Ca V 1.2, n = 22, N = 3; Ca V 1.3, n = 16, N = 3) and old (Ca V 1.2, n= 33, N = 3; Ca V 1.3, n = 23, N = 3) cells. Statistical comparisons used a two-tail t-test.

    Techniques Used: Western Blot, Expressing, MANN-WHITNEY, Labeling

    (A, B) Representative super-resolution (GSD) images of Ca V 1.2 and Ca V 1.3 channels in young and old pacemaker cells. Panels to the right of each image are zoom in. (C) Average cluster area for Ca V 1.2 and Ca V 1.3 channel clusters in young and old pacemaker cells. (D) Comparison of Ca V 1.2 and Ca V 1.3 cluster density between young and old cells. (E , F) Comparison of the frequency distributions of the area of Ca V 1.2 and Ca V 1.3 channel clusters between young and old cells. In all the scattered plots bars represent the mean and error bars the SEM. Statistical comparisons used a two-tail t-test comparing a population of n = 8 cells, N = 3 mice for Ca V 1.2 young; n = 12 cells, N = 4 mice for Ca V 1.3 young; n = 6 cells, N = 3 mice for Ca V 1.2 old; and n = 8 cells, N = 3 mice for Ca V 1.3 old.
    Figure Legend Snippet: (A, B) Representative super-resolution (GSD) images of Ca V 1.2 and Ca V 1.3 channels in young and old pacemaker cells. Panels to the right of each image are zoom in. (C) Average cluster area for Ca V 1.2 and Ca V 1.3 channel clusters in young and old pacemaker cells. (D) Comparison of Ca V 1.2 and Ca V 1.3 cluster density between young and old cells. (E , F) Comparison of the frequency distributions of the area of Ca V 1.2 and Ca V 1.3 channel clusters between young and old cells. In all the scattered plots bars represent the mean and error bars the SEM. Statistical comparisons used a two-tail t-test comparing a population of n = 8 cells, N = 3 mice for Ca V 1.2 young; n = 12 cells, N = 4 mice for Ca V 1.3 young; n = 6 cells, N = 3 mice for Ca V 1.2 old; and n = 8 cells, N = 3 mice for Ca V 1.3 old.

    Techniques Used:

    antisyntaxin3 rabbit polyclonal antibody  (Alomone Labs)


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    Alomone Labs antisyntaxin3 rabbit polyclonal antibody
    Antisyntaxin3 Rabbit Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    andkv12 1  (Alomone Labs)


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    Alomone Labs andkv12 1
    Andkv12 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    antibody against kv12 1  (Alomone Labs)


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    Alomone Labs antibody against kv12 1
    Antibody Against Kv12 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    alx 215 053 r100 rabbit polyclonal  (Alomone Labs)


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    Alomone Labs alx 215 053 r100 rabbit polyclonal
    Alx 215 053 R100 Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti kv12 1  (Alomone Labs)


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    Alomone Labs anti kv12 1
    Anti Kv12 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal anti neuregulin 1 nrg1 type iii  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti neuregulin 1 nrg1 type iii
    Y172 immunoreactivity colocalizes with S1R and Kv2.1 in C-boutons. (A1–D4) Representative confocal images of adult (P75) mouse spinal cord MNs double immunostained with antibodies against either S1R or Kv2.1 (red) and Y172 (green), as indicated in the panels; sections were counterstained with fluorescent Nissl stain (blue) for MN visualization. The areas delimited by dotted-line squares in (A4,C4) are shown at higher magnification in (B1–B4,D1–D4) , respectively. (E–H) Pixel profile analysis (F,H ) along the lines depicted in (E,G) , which were obtained from MNs immunolabeled with Y172 (green) and anti-S1R or Kv2.1 (red) antibodies; fluorescent Nissl staining (blue) for MN visualization can be seen in (E) . Note the overlap between the Y172 and S1R (F) or Kv2.1 (H) signals. (I,J) Squashed MNs immunolabeled with <t>anti-NRG1</t> (I) and Y172 (J) antibodies; the circled profiles are shown at higher magnification in the insets; note that Y172-positive profiles were smaller and displayed more compact patterns than NRG1 clusters. Scale bars: A4,C4 = 10 μm (valid for A1–A3,C1–C3 ); B4,D4 = 1 μm (valid for B1–B3,D1–D3 ); G = 1 μm (valid for E ); and J = 20 μm (valid for I ).
    Rabbit Polyclonal Anti Neuregulin 1 Nrg1 Type Iii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "The Y172 Monoclonal Antibody Against p-c-Jun (Ser63) Is a Marker of the Postsynaptic Compartment of C-Type Cholinergic Afferent Synapses on Motoneurons"

    Article Title: The Y172 Monoclonal Antibody Against p-c-Jun (Ser63) Is a Marker of the Postsynaptic Compartment of C-Type Cholinergic Afferent Synapses on Motoneurons

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2019.00582

    Y172 immunoreactivity colocalizes with S1R and Kv2.1 in C-boutons. (A1–D4) Representative confocal images of adult (P75) mouse spinal cord MNs double immunostained with antibodies against either S1R or Kv2.1 (red) and Y172 (green), as indicated in the panels; sections were counterstained with fluorescent Nissl stain (blue) for MN visualization. The areas delimited by dotted-line squares in (A4,C4) are shown at higher magnification in (B1–B4,D1–D4) , respectively. (E–H) Pixel profile analysis (F,H ) along the lines depicted in (E,G) , which were obtained from MNs immunolabeled with Y172 (green) and anti-S1R or Kv2.1 (red) antibodies; fluorescent Nissl staining (blue) for MN visualization can be seen in (E) . Note the overlap between the Y172 and S1R (F) or Kv2.1 (H) signals. (I,J) Squashed MNs immunolabeled with anti-NRG1 (I) and Y172 (J) antibodies; the circled profiles are shown at higher magnification in the insets; note that Y172-positive profiles were smaller and displayed more compact patterns than NRG1 clusters. Scale bars: A4,C4 = 10 μm (valid for A1–A3,C1–C3 ); B4,D4 = 1 μm (valid for B1–B3,D1–D3 ); G = 1 μm (valid for E ); and J = 20 μm (valid for I ).
    Figure Legend Snippet: Y172 immunoreactivity colocalizes with S1R and Kv2.1 in C-boutons. (A1–D4) Representative confocal images of adult (P75) mouse spinal cord MNs double immunostained with antibodies against either S1R or Kv2.1 (red) and Y172 (green), as indicated in the panels; sections were counterstained with fluorescent Nissl stain (blue) for MN visualization. The areas delimited by dotted-line squares in (A4,C4) are shown at higher magnification in (B1–B4,D1–D4) , respectively. (E–H) Pixel profile analysis (F,H ) along the lines depicted in (E,G) , which were obtained from MNs immunolabeled with Y172 (green) and anti-S1R or Kv2.1 (red) antibodies; fluorescent Nissl staining (blue) for MN visualization can be seen in (E) . Note the overlap between the Y172 and S1R (F) or Kv2.1 (H) signals. (I,J) Squashed MNs immunolabeled with anti-NRG1 (I) and Y172 (J) antibodies; the circled profiles are shown at higher magnification in the insets; note that Y172-positive profiles were smaller and displayed more compact patterns than NRG1 clusters. Scale bars: A4,C4 = 10 μm (valid for A1–A3,C1–C3 ); B4,D4 = 1 μm (valid for B1–B3,D1–D3 ); G = 1 μm (valid for E ); and J = 20 μm (valid for I ).

    Techniques Used: Staining, Immunolabeling

    Changes in Y172 immunoreactivity in MNs from mutant mice (P60) overexpressing NRG1 type III. (A–F) The density (per 100 μm 2 MN soma, A,B ) and size (in μm 2 , C,D ) of total (A,C) and peripheral (periph., B,D ) Y172-positive profiles and the percentage of these profiles showing a spatial association with VAChT-positive C-boutons (E) and NRG1 type III-positive spots (F) in MNs from WT and NRG1 type III-overexpressing mice. Note that NRG1 type III overexpression was associated with a prominent decrease in the density of total Y172-positive profiles (A) and a significant increase in the number of those located peripherally (B) in MNs; the area of both total and peripheral Y172-positive profiles was dramatically increased in MNs from NRG1 type III-overexpressing animals (C,D) . Additionally, the percentage of Y172-positive profiles showing a close association with VAChT- (E) or NRG1 type III-positive (F) spots significantly increased or decreased, respectively, in MNs from NRG1 type III-overexpressing animals; 10–15 randomly selected MNs from 3 to 4 mice per condition were analyzed; * p < 0.05 and *** p < 0.001 vs. WT; student’s t -test). ( G1–G4) Representative confocal micrographs of an NRG1 type III-overexpressing MN immunostained with Y172 (green) and anti-NRG1 type III (red) antibodies and counterstained with fluorescent Nissl stain (blue) for neuron visualization. Note that, compared to MNs of adult CD1 mice (see, for instance, or ), MNs overexpressing NRG1 type III exhibit an enlargement of Y172-positive profiles located in the periphery of the cell body, which correlated with the redundant and expanded SSCs previously described in Salvany et al. ; note also the expansion of NRG1 type III immunolabeling peripherally located in MN soma. (H1–H3) A higher magnification image of the area delimited in (G4) by the dotted-line rectangle corresponding to Y172, NRG1 type III and merged channels, as indicated, is shown. Scale bars: G4 = 10 μm (valid for G1-G3 ); H3 = 2.5 μm (valid for H1, H2 ).
    Figure Legend Snippet: Changes in Y172 immunoreactivity in MNs from mutant mice (P60) overexpressing NRG1 type III. (A–F) The density (per 100 μm 2 MN soma, A,B ) and size (in μm 2 , C,D ) of total (A,C) and peripheral (periph., B,D ) Y172-positive profiles and the percentage of these profiles showing a spatial association with VAChT-positive C-boutons (E) and NRG1 type III-positive spots (F) in MNs from WT and NRG1 type III-overexpressing mice. Note that NRG1 type III overexpression was associated with a prominent decrease in the density of total Y172-positive profiles (A) and a significant increase in the number of those located peripherally (B) in MNs; the area of both total and peripheral Y172-positive profiles was dramatically increased in MNs from NRG1 type III-overexpressing animals (C,D) . Additionally, the percentage of Y172-positive profiles showing a close association with VAChT- (E) or NRG1 type III-positive (F) spots significantly increased or decreased, respectively, in MNs from NRG1 type III-overexpressing animals; 10–15 randomly selected MNs from 3 to 4 mice per condition were analyzed; * p < 0.05 and *** p < 0.001 vs. WT; student’s t -test). ( G1–G4) Representative confocal micrographs of an NRG1 type III-overexpressing MN immunostained with Y172 (green) and anti-NRG1 type III (red) antibodies and counterstained with fluorescent Nissl stain (blue) for neuron visualization. Note that, compared to MNs of adult CD1 mice (see, for instance, or ), MNs overexpressing NRG1 type III exhibit an enlargement of Y172-positive profiles located in the periphery of the cell body, which correlated with the redundant and expanded SSCs previously described in Salvany et al. ; note also the expansion of NRG1 type III immunolabeling peripherally located in MN soma. (H1–H3) A higher magnification image of the area delimited in (G4) by the dotted-line rectangle corresponding to Y172, NRG1 type III and merged channels, as indicated, is shown. Scale bars: G4 = 10 μm (valid for G1-G3 ); H3 = 2.5 μm (valid for H1, H2 ).

    Techniques Used: Mutagenesis, Over Expression, Staining, Immunolabeling

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    Structured Review

    Alomone Labs ca v 1 3 rabbit
    Ca V 1 3 Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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