anti mouse human synapsin1 2  (Synaptic Systems)


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    Synaptic Systems anti mouse human synapsin1 2
    Anti Mouse Human Synapsin1 2, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse human synapsin1 2/product/Synaptic Systems
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse human synapsin1 2 - by Bioz Stars, 2022-12
    90/100 stars

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    Synaptic Systems anti mouse human synapsin1 2
    Anti Mouse Human Synapsin1 2, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse human synapsin1 2/product/Synaptic Systems
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse human synapsin1 2 - by Bioz Stars, 2022-12
    90/100 stars
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    Alomone Labs apr 106
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    Alomone Labs anti p2y6 receptor fitc
    Decrease of <t>P2Y6</t> Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a <t>FITC-conjugated</t> antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p
    Anti P2y6 Receptor Fitc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p2y6 receptor fitc/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p2y6 receptor fitc - by Bioz Stars, 2022-12
    93/100 stars
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    Decrease of P2Y6 Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a FITC-conjugated antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p

    Journal: Frontiers in Immunology

    Article Title: The Inhibitory Effect of Curosurf® and Alveofact® on the Formation of Neutrophil Extracellular Traps

    doi: 10.3389/fimmu.2020.582895

    Figure Lengend Snippet: Decrease of P2Y6 Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a FITC-conjugated antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p

    Article Snippet: Cells were washed twice with PBS (Thermo Fischer, Waltham, MA, USA) and labeled with propidium iodide (PI) and Annexin-V-FITC (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) or anti-CD11b-VioBlue (mAb REA713, IgG1) and anti-CD66b-APC (mAb REA306, IgG1) antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) or anti-P2Y6 Receptor-FITC (Alomone Labs, Jerusalem, Israel) as described in the manufacturer’s protocols.

    Techniques: Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay