apc101  (Alomone Labs)


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    Name:
    Anti Kv1 3 KCNA3 extracellular Antibody
    Description:
    Anti KV1 3 KCNA3 extracellular Antibody APC 101 is a highly specific antibody directed against an extracellular epitope of the human protein The antibody can be used in western blot immunohistochemistry live cell imaging and indirect flow cytometry applications It has been designed to recognize KV1 3 potassium channel from human mouse and rat samples
    Catalog Number:
    APC-101
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs apc101
    Anti Kv1 3 KCNA3 extracellular Antibody
    Anti KV1 3 KCNA3 extracellular Antibody APC 101 is a highly specific antibody directed against an extracellular epitope of the human protein The antibody can be used in western blot immunohistochemistry live cell imaging and indirect flow cytometry applications It has been designed to recognize KV1 3 potassium channel from human mouse and rat samples
    https://www.bioz.com/result/apc101/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apc101 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia
    Article Snippet: .. Sample immunofluorescence staining of 14‐μM thick Cx3CR1+/EGFP (green) MCAO brain coronal brain sections from the 6‐mm depth showing no immunoreactivity with the rabbit a polyclonal rabbit anti‐Kv1.3 antibody (Alomone, APC‐101, 1:1000) in the ipsilateral brain after preincubation with the blocking peptide for 45 minutes at RT in contrast to clear staining without the blocking peptide. ..

    Article Title: Contribution of the Potassium Channels KV1.3 and KCa3.1 to Smooth Muscle Cell Proliferation in Growing Collateral Arteries
    Article Snippet: .. For immunofluorescence staining, cryofixed tissue sections (10 μm) were stained with a rabbit anti-KV 1.3 (catalog number APC-101) or a rabbit anti-KCa 3.1 (catalog number APC-064) antibody (both from Alomone Labs) followed by a goat anti-rabbit IgG Alexa fluor 488-conjugated antibody (catalog number 711-545-153, Jackson ImmunoResearch) together with a Cy3-conjugated mouse anti-αSM-actin antibody (catalog number C6198, Sigma-Aldrich) and an Alexa fluor 647-conjugated rat anti-CD31 antibody (catalog number 102515, BioLegend), followed by DAPI counter staining (catalog number 62248, Thermo Fisher Scientific). ..

    Staining:

    Article Title: Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia
    Article Snippet: .. Sample immunofluorescence staining of 14‐μM thick Cx3CR1+/EGFP (green) MCAO brain coronal brain sections from the 6‐mm depth showing no immunoreactivity with the rabbit a polyclonal rabbit anti‐Kv1.3 antibody (Alomone, APC‐101, 1:1000) in the ipsilateral brain after preincubation with the blocking peptide for 45 minutes at RT in contrast to clear staining without the blocking peptide. ..

    Article Title: Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia
    Article Snippet: .. Kv1.3 was stained with a polyclonal rabbit anti‐Kv1.3 antibody (Alomone, APC‐101, 1:1,000). ..

    Article Title: Contribution of the Potassium Channels KV1.3 and KCa3.1 to Smooth Muscle Cell Proliferation in Growing Collateral Arteries
    Article Snippet: .. For immunofluorescence staining, cryofixed tissue sections (10 μm) were stained with a rabbit anti-KV 1.3 (catalog number APC-101) or a rabbit anti-KCa 3.1 (catalog number APC-064) antibody (both from Alomone Labs) followed by a goat anti-rabbit IgG Alexa fluor 488-conjugated antibody (catalog number 711-545-153, Jackson ImmunoResearch) together with a Cy3-conjugated mouse anti-αSM-actin antibody (catalog number C6198, Sigma-Aldrich) and an Alexa fluor 647-conjugated rat anti-CD31 antibody (catalog number 102515, BioLegend), followed by DAPI counter staining (catalog number 62248, Thermo Fisher Scientific). ..

    Blocking Assay:

    Article Title: Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4‐mediated calcium entry in microglia
    Article Snippet: .. Sample immunofluorescence staining of 14‐μM thick Cx3CR1+/EGFP (green) MCAO brain coronal brain sections from the 6‐mm depth showing no immunoreactivity with the rabbit a polyclonal rabbit anti‐Kv1.3 antibody (Alomone, APC‐101, 1:1000) in the ipsilateral brain after preincubation with the blocking peptide for 45 minutes at RT in contrast to clear staining without the blocking peptide. ..

    Electrophoresis:

    Article Title: Novel Mitochondria-Targeted Furocoumarin Derivatives as Possible Anti-Cancer Agents
    Article Snippet: .. After separation by electrophoresis, gels were blotted overnight at 4°C onto polyvinylidene fluoride membranes and then membranes were blocked with a 10% solution of defatted milk and were incubated with the following primary antibodies overnight at 4°C: anti-Kv1.3 (1:200, rabbit polyclonal, Alomone Labs APC-101); anti-GAPDH (1:1,000, mouse monoclonal, Millipore MAB374). ..

    Incubation:

    Article Title: Novel Mitochondria-Targeted Furocoumarin Derivatives as Possible Anti-Cancer Agents
    Article Snippet: .. After separation by electrophoresis, gels were blotted overnight at 4°C onto polyvinylidene fluoride membranes and then membranes were blocked with a 10% solution of defatted milk and were incubated with the following primary antibodies overnight at 4°C: anti-Kv1.3 (1:200, rabbit polyclonal, Alomone Labs APC-101); anti-GAPDH (1:1,000, mouse monoclonal, Millipore MAB374). ..

    Article Title: Loureirin B Exerts its Immunosuppressive Effects by Inhibiting STIM1/Orai1 and KV1.3 Channels
    Article Snippet: .. The membranes were blocked with 10% nonfat milk and incubated at 4°C overnight with the following antibodies: rabbit polyclonal antibody against KV 1.3 (1:1,000, Cat. APC101; alomone labs, Jerusalem, Israel) and rabbit polyclonal antibody against β-actin (1:1,000, Cat. AC006; Abclonal, Boston, United States). ..

    Article Title: Insight into the mechanism of cytotoxicity of membrane-permeant psoralenic Kv1.3 channel inhibitors by chemical dissection of a novel member of the family
    Article Snippet: .. Membranes were incubated overnight at 4 °C with the following primary antibodies: anti-Kv1.3 (1:200 in TBS, rabbit polyclonal, Alomone Labs APC-101); anti-NDUFA9 (1:1000 in 5% skim milk, mouse monoclonal, Abcam ab14713). ..

    Article Title: KCNE4-dependent functional consequences of Kv1.3-related leukocyte physiology
    Article Snippet: .. Next, the cells were incubated with anti-KCNE4 (1/50, Proteintech) or anti-Kv1.3 extracellular (1/150, Alomone) antibodies with 10% goat serum in 0.05% Triton X-100 PBS-K+ for 1 h 45 min at RT. ..

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    Alomone Labs xe991 dihydrochloride
    Kv7.4 is a regulation target of 5-HT 1A auto-inhibition. (A1) 5-HT 1A receptor agonist 8-OH-DPAT increased the Kv7 current in DRN 5-HT neurons of WT mice and the effect was reversed by <t>XE991.</t> (A2) Voltage clamping protocol and the corresponding traces recorded from (A1) . (A3) Summarized data for experiments shown in (A1) (paired samples test, ** P
    Xe991 Dihydrochloride, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xe991 dihydrochloride/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xe991 dihydrochloride - by Bioz Stars, 2021-09
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    94
    Alomone Labs anti kv1 3
    Inhibition of mitochondrial <t>Kv1.3</t> kills PANC-1 cells. (A) PANC-1 cells were treated or left untreated with different membrane permeant Kv1.3 inhibitors (PAPTP 10 µM, PCARBTP 10 µM, and clofazimine 20 µM) for 24 h. Cell death was determined by staining with an Alexa568 coupled Annexin V by fluorescent microscopy. The images are representative of three different replicates. (B) Quantification of Annexin V-positive cells from the experiments shown in (A) : represented is the percentage of Annexin V-positive cells ± SEM ( n = 3; ** p
    Anti Kv1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv1 3 - by Bioz Stars, 2021-09
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    90
    Alomone Labs retigabine dihydrochloride
    KCNQ activation increases flurothyl-induced seizure latencies in Kcnq1 mice and decreases spontaneous seizure frequency in Kcna1 −/− mice. ( A ) Administration of RTG (10 mg·kg −1 , ip) had no significant effect on latency to flurothyl-induced myoclonic jerks (MJ) in Tac:N:NIHS-BC Kcna1 +/+ and Kcna1 −/− mice. However, RTG decreased latency to myoclonic jerk in Kcnq1 A340E/A340E mutant mice ( B ). ( C ) Administration of RTG (10 mg·kg −1 , ip) had no significant effect on latency to flurothyl-induced generalized tonic-clonic seizures (GTCS) in Tac:N:NIHS-BC Kcna1 +/+ and Kcna1 −/− mice. However, RTG significantly increased the latency to GTCS in Kcnq1 strain (C57BL/6) mice ( D ). ( E ) EEG traces showing representative spontaneous seizure activity in Kcna1 −/− mice after receiving either VEH or RTG (10 mg·kg −1 ). ( F ) Analysis of spontaneous seizure frequency in Kcna1 −/− mice (n = 6) showed a significant decrease after treatment with 10 mg·kg −1 RTG. ( G ) Average spontaneous seizure durations were similar in Kcna1 −/− mice that exhibited seizures after VEH and 10 mg·kg −1 RTG administration (n = 3). For ( A-D ): *, P ≤ 0.05; **, P ≤ 0.01 (two-tailed unpaired Student’s t -test; n = 6–11 per treatment). For ( F ) and ( G ): *, P ≤ 0.05 (two-tailed paired Student’s t -test). Abbreviations: LT, left temporal; VEH, vehicle; RTG, <t>retigabine;</t> NS, not significant compared to VEH control.
    Retigabine Dihydrochloride, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/retigabine dihydrochloride/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    Kv7.4 is a regulation target of 5-HT 1A auto-inhibition. (A1) 5-HT 1A receptor agonist 8-OH-DPAT increased the Kv7 current in DRN 5-HT neurons of WT mice and the effect was reversed by XE991. (A2) Voltage clamping protocol and the corresponding traces recorded from (A1) . (A3) Summarized data for experiments shown in (A1) (paired samples test, ** P

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Selective Modulation of K+ Channel Kv7.4 Significantly Affects the Excitability of DRN 5-HT Neurons

    doi: 10.3389/fncel.2017.00405

    Figure Lengend Snippet: Kv7.4 is a regulation target of 5-HT 1A auto-inhibition. (A1) 5-HT 1A receptor agonist 8-OH-DPAT increased the Kv7 current in DRN 5-HT neurons of WT mice and the effect was reversed by XE991. (A2) Voltage clamping protocol and the corresponding traces recorded from (A1) . (A3) Summarized data for experiments shown in (A1) (paired samples test, ** P

    Article Snippet: XE991 dihydrochloride was from the Alomone Labs (Jerusalem, Israel).

    Techniques: Inhibition, Mouse Assay

    Fasudil potently augments Kv7.4 currents in DRN 5-HT neurons. (A1) Sample time course shows that fasudil (30 μM) augments Kv7 current, which is completely blocked by XE991 (3 μM) in neurons from WT mice. (A2) Voltage clamping protocol and the corresponding current traces recorded from (A1) . (A3) Summarized data for the experiment shown in (A1) (Wilcoxon Singed Ranks Test, * P

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Selective Modulation of K+ Channel Kv7.4 Significantly Affects the Excitability of DRN 5-HT Neurons

    doi: 10.3389/fncel.2017.00405

    Figure Lengend Snippet: Fasudil potently augments Kv7.4 currents in DRN 5-HT neurons. (A1) Sample time course shows that fasudil (30 μM) augments Kv7 current, which is completely blocked by XE991 (3 μM) in neurons from WT mice. (A2) Voltage clamping protocol and the corresponding current traces recorded from (A1) . (A3) Summarized data for the experiment shown in (A1) (Wilcoxon Singed Ranks Test, * P

    Article Snippet: XE991 dihydrochloride was from the Alomone Labs (Jerusalem, Israel).

    Techniques: Mouse Assay

    Kv7.4 activity modulates the excitability of the DRN 5-HT neurons. (A,B) Effects of fasudil on the elicited firing activity of DRN 5-HT neurons from WT mice and Kv7.4 −/− mice. (A1) Fasudil (30 μM) significantly hyperpolarized resting membrane potential (RMP) and the effect completely reversed by XE991 (3 μM) in WT mice. (A2) Typical traces with firing activity elicited by depolarizing 250 pA current injection, recorded from a neuron of WT mice. (A3) Summary of RMP in response to fasudil (30 μM) in WT DRN 5-HT neurons (paired samples test, *** P

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Selective Modulation of K+ Channel Kv7.4 Significantly Affects the Excitability of DRN 5-HT Neurons

    doi: 10.3389/fncel.2017.00405

    Figure Lengend Snippet: Kv7.4 activity modulates the excitability of the DRN 5-HT neurons. (A,B) Effects of fasudil on the elicited firing activity of DRN 5-HT neurons from WT mice and Kv7.4 −/− mice. (A1) Fasudil (30 μM) significantly hyperpolarized resting membrane potential (RMP) and the effect completely reversed by XE991 (3 μM) in WT mice. (A2) Typical traces with firing activity elicited by depolarizing 250 pA current injection, recorded from a neuron of WT mice. (A3) Summary of RMP in response to fasudil (30 μM) in WT DRN 5-HT neurons (paired samples test, *** P

    Article Snippet: XE991 dihydrochloride was from the Alomone Labs (Jerusalem, Israel).

    Techniques: Activity Assay, Mouse Assay, Injection

    5-HT augments Kv7.4 currents in DRN 5-HT neurons. (A1) Sample time course shows that 5-HT (30 μM) induced a sharp augmentation of Kv7 current and the augmentation was completely reversed by XE991 (3 μM) in DRN 5-HT neurons of WT mice. (A2) Voltage clamping protocol and representative current traces recorded from (A1) . (A3) Summarized data for experiments shown in (A1) (Wilcoxon Signed Ranks Test, *** P

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Selective Modulation of K+ Channel Kv7.4 Significantly Affects the Excitability of DRN 5-HT Neurons

    doi: 10.3389/fncel.2017.00405

    Figure Lengend Snippet: 5-HT augments Kv7.4 currents in DRN 5-HT neurons. (A1) Sample time course shows that 5-HT (30 μM) induced a sharp augmentation of Kv7 current and the augmentation was completely reversed by XE991 (3 μM) in DRN 5-HT neurons of WT mice. (A2) Voltage clamping protocol and representative current traces recorded from (A1) . (A3) Summarized data for experiments shown in (A1) (Wilcoxon Signed Ranks Test, *** P

    Article Snippet: XE991 dihydrochloride was from the Alomone Labs (Jerusalem, Israel).

    Techniques: Mouse Assay

    Inhibition of mitochondrial Kv1.3 kills PANC-1 cells. (A) PANC-1 cells were treated or left untreated with different membrane permeant Kv1.3 inhibitors (PAPTP 10 µM, PCARBTP 10 µM, and clofazimine 20 µM) for 24 h. Cell death was determined by staining with an Alexa568 coupled Annexin V by fluorescent microscopy. The images are representative of three different replicates. (B) Quantification of Annexin V-positive cells from the experiments shown in (A) : represented is the percentage of Annexin V-positive cells ± SEM ( n = 3; ** p

    Journal: Frontiers in Oncology

    Article Title: Regulation of Proliferation by a Mitochondrial Potassium Channel in Pancreatic Ductal Adenocarcinoma Cells

    doi: 10.3389/fonc.2017.00239

    Figure Lengend Snippet: Inhibition of mitochondrial Kv1.3 kills PANC-1 cells. (A) PANC-1 cells were treated or left untreated with different membrane permeant Kv1.3 inhibitors (PAPTP 10 µM, PCARBTP 10 µM, and clofazimine 20 µM) for 24 h. Cell death was determined by staining with an Alexa568 coupled Annexin V by fluorescent microscopy. The images are representative of three different replicates. (B) Quantification of Annexin V-positive cells from the experiments shown in (A) : represented is the percentage of Annexin V-positive cells ± SEM ( n = 3; ** p

    Article Snippet: After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-Kv1.3 (1:200, rabbit polyclonal; Alomone Labs APC-101); anti-GAPDH (1:1,000, mouse monoclonal; Millipore MAB374).

    Techniques: Inhibition, Staining, Microscopy

    Sublethal inhibition of mitochondrial Kv1.3 leads to cell cycle alterations. (A) FACS analysis of cell cycle of PANC-1 cells untreated or treated with clofazimine 1 µM and ShK 100 nM for 24 h. The distribution was determined by staining cells with 50 µg/mL Propidium Iodide and the acquisition was performed by a FACSantoII (Beckton Dickson). The plots are representative of three separated determinations. Light blue peaks represent apoptotic cells, while red peaks represent cells in G1 and G2/M phases. S phase is represented by the area with blue straight lines. A sub-G1 peak is due to dead cells. In the graph of untreated cells the labels and the arrows identify the different populations that are reported. (B) Cell cycle distribution in PANC-1 cells pretreated (lower panels) or not (upper panels) with 50 µM Mitotempo or 20 mM N-acetylcysteine (NAC) for 1 h, before the addition of mitochondrial Kv1.3 inhibitors PAPTP and PCARBTP (both at 100 nM) for 24 h. The experiment and the analysis have been performed as in (A) . The plots are representative of three separate measurements.

    Journal: Frontiers in Oncology

    Article Title: Regulation of Proliferation by a Mitochondrial Potassium Channel in Pancreatic Ductal Adenocarcinoma Cells

    doi: 10.3389/fonc.2017.00239

    Figure Lengend Snippet: Sublethal inhibition of mitochondrial Kv1.3 leads to cell cycle alterations. (A) FACS analysis of cell cycle of PANC-1 cells untreated or treated with clofazimine 1 µM and ShK 100 nM for 24 h. The distribution was determined by staining cells with 50 µg/mL Propidium Iodide and the acquisition was performed by a FACSantoII (Beckton Dickson). The plots are representative of three separated determinations. Light blue peaks represent apoptotic cells, while red peaks represent cells in G1 and G2/M phases. S phase is represented by the area with blue straight lines. A sub-G1 peak is due to dead cells. In the graph of untreated cells the labels and the arrows identify the different populations that are reported. (B) Cell cycle distribution in PANC-1 cells pretreated (lower panels) or not (upper panels) with 50 µM Mitotempo or 20 mM N-acetylcysteine (NAC) for 1 h, before the addition of mitochondrial Kv1.3 inhibitors PAPTP and PCARBTP (both at 100 nM) for 24 h. The experiment and the analysis have been performed as in (A) . The plots are representative of three separate measurements.

    Article Snippet: After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-Kv1.3 (1:200, rabbit polyclonal; Alomone Labs APC-101); anti-GAPDH (1:1,000, mouse monoclonal; Millipore MAB374).

    Techniques: Inhibition, FACS, Staining

    Potassium channel Kv1.3 is expressed in PANC-1 cells. (A) Kv1.3 expression was determined by Western Blot in PANC-1 cells. 60 µg of total protein extract were loaded into a SDS gel and blotted onto a polyvinylidene fluoride (PVDF) membrane. Kv1.3 band was evaluated by immunoblotting with a specific antibody. GAPDH was used as loading control. The blot is a representative image of three different observations. (B) Inhibition of mitochondrial Kv1.3 by different concentration of membrane permeant blockers resulted in a reduction of the MTS signal from PANC-1 cells. Values are reported as percentage respect to untreated sample ± SEM. All compounds were added for 24 h. Staurosporine was used as positive control ( n = 3; *** p

    Journal: Frontiers in Oncology

    Article Title: Regulation of Proliferation by a Mitochondrial Potassium Channel in Pancreatic Ductal Adenocarcinoma Cells

    doi: 10.3389/fonc.2017.00239

    Figure Lengend Snippet: Potassium channel Kv1.3 is expressed in PANC-1 cells. (A) Kv1.3 expression was determined by Western Blot in PANC-1 cells. 60 µg of total protein extract were loaded into a SDS gel and blotted onto a polyvinylidene fluoride (PVDF) membrane. Kv1.3 band was evaluated by immunoblotting with a specific antibody. GAPDH was used as loading control. The blot is a representative image of three different observations. (B) Inhibition of mitochondrial Kv1.3 by different concentration of membrane permeant blockers resulted in a reduction of the MTS signal from PANC-1 cells. Values are reported as percentage respect to untreated sample ± SEM. All compounds were added for 24 h. Staurosporine was used as positive control ( n = 3; *** p

    Article Snippet: After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-Kv1.3 (1:200, rabbit polyclonal; Alomone Labs APC-101); anti-GAPDH (1:1,000, mouse monoclonal; Millipore MAB374).

    Techniques: Expressing, Western Blot, SDS-Gel, Inhibition, Concentration Assay, Positive Control

    Characterization of the Kv1.3 and Kv1.5 mutant channels containing the YS segment. A , average normalized activation and inactivation curves are shown as conductance-voltage relationships for Kv1.3, Kv1.5, the truncated Kv1.3-YS channel, and the chimeras Kv1.5-YS 532 and Kv1.5-YS 613 . All datasets were fitted to Boltzmann functions. Each data point is the mean ± S.E. of 6–11 cells. B , confocal images of non-permeabilized cells transfected with Kv1.3-YS-Cherry, Kv1.5-YS 532 -EGFP, and Kv1.5-YS 613 -EGFP. An extracellular anti-Kv1.3 antibody was used to label Kv1.3-YS ( green ), whereas the extracellular anti-Kv1.5 antibody was used for Kv1.5-YS 532 and Kv1.5-YS 613 chimeras ( red ). Nuclei were stained by Hoechst ( blue ). C , proliferation rate of the indicated channels or GFP-transfected cells (control) was determined by measuring EdU incorporation. Significant differences when comparing to Kv1.3 (*) or to control (#) are indicated. Statistical analysis was performed with one-way ANOVA followed by a Tukey's HSD multiple comparison. Each bar is the average of 9–15 determinations from 5 different assays. D , the average peak current amplitude obtained in cell-attached experiments for Kv1.5 channels and all the Kv1.5 chimeras was plotted against the % of the channels expressed at the plasma membrane ( upper graph ) or their normalized effect on proliferation (taking 100% as the proliferation rate of GFP-transfected HEK cells, lower graph ). The correlation between expression and current was fit to a linear regression curve ( y = 18.54 + 0.0066x, R 2 = 0.85, p = 0.008), but there was no correlation between proliferation and current amplitude ( R 2 = 0.23, p = 0.19).

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular Determinants of Kv1.3 Potassium Channels-induced Proliferation *

    doi: 10.1074/jbc.M115.678995

    Figure Lengend Snippet: Characterization of the Kv1.3 and Kv1.5 mutant channels containing the YS segment. A , average normalized activation and inactivation curves are shown as conductance-voltage relationships for Kv1.3, Kv1.5, the truncated Kv1.3-YS channel, and the chimeras Kv1.5-YS 532 and Kv1.5-YS 613 . All datasets were fitted to Boltzmann functions. Each data point is the mean ± S.E. of 6–11 cells. B , confocal images of non-permeabilized cells transfected with Kv1.3-YS-Cherry, Kv1.5-YS 532 -EGFP, and Kv1.5-YS 613 -EGFP. An extracellular anti-Kv1.3 antibody was used to label Kv1.3-YS ( green ), whereas the extracellular anti-Kv1.5 antibody was used for Kv1.5-YS 532 and Kv1.5-YS 613 chimeras ( red ). Nuclei were stained by Hoechst ( blue ). C , proliferation rate of the indicated channels or GFP-transfected cells (control) was determined by measuring EdU incorporation. Significant differences when comparing to Kv1.3 (*) or to control (#) are indicated. Statistical analysis was performed with one-way ANOVA followed by a Tukey's HSD multiple comparison. Each bar is the average of 9–15 determinations from 5 different assays. D , the average peak current amplitude obtained in cell-attached experiments for Kv1.5 channels and all the Kv1.5 chimeras was plotted against the % of the channels expressed at the plasma membrane ( upper graph ) or their normalized effect on proliferation (taking 100% as the proliferation rate of GFP-transfected HEK cells, lower graph ). The correlation between expression and current was fit to a linear regression curve ( y = 18.54 + 0.0066x, R 2 = 0.85, p = 0.008), but there was no correlation between proliferation and current amplitude ( R 2 = 0.23, p = 0.19).

    Article Snippet: Non-permeabilized cells were incubated with anti-Kv1.3 or anti Kv1.5 extracellular primary antibodies (APC101 or APC150, Alomone Labs), whereas permeabilized cells were incubated with anti-Kv1.3 COOH (75-009, NeuroMab) or anti-Kv1.5 COOH (APC004, Alomone Labs), all at a final concentration of 1:50.

    Techniques: Mutagenesis, Activation Assay, Transfection, Staining, Expressing

    KCNQ activation increases flurothyl-induced seizure latencies in Kcnq1 mice and decreases spontaneous seizure frequency in Kcna1 −/− mice. ( A ) Administration of RTG (10 mg·kg −1 , ip) had no significant effect on latency to flurothyl-induced myoclonic jerks (MJ) in Tac:N:NIHS-BC Kcna1 +/+ and Kcna1 −/− mice. However, RTG decreased latency to myoclonic jerk in Kcnq1 A340E/A340E mutant mice ( B ). ( C ) Administration of RTG (10 mg·kg −1 , ip) had no significant effect on latency to flurothyl-induced generalized tonic-clonic seizures (GTCS) in Tac:N:NIHS-BC Kcna1 +/+ and Kcna1 −/− mice. However, RTG significantly increased the latency to GTCS in Kcnq1 strain (C57BL/6) mice ( D ). ( E ) EEG traces showing representative spontaneous seizure activity in Kcna1 −/− mice after receiving either VEH or RTG (10 mg·kg −1 ). ( F ) Analysis of spontaneous seizure frequency in Kcna1 −/− mice (n = 6) showed a significant decrease after treatment with 10 mg·kg −1 RTG. ( G ) Average spontaneous seizure durations were similar in Kcna1 −/− mice that exhibited seizures after VEH and 10 mg·kg −1 RTG administration (n = 3). For ( A-D ): *, P ≤ 0.05; **, P ≤ 0.01 (two-tailed unpaired Student’s t -test; n = 6–11 per treatment). For ( F ) and ( G ): *, P ≤ 0.05 (two-tailed paired Student’s t -test). Abbreviations: LT, left temporal; VEH, vehicle; RTG, retigabine; NS, not significant compared to VEH control.

    Journal: Epilepsia

    Article Title: Pharmacogenetics of KCNQ channel activation in two potassium channelopathy mouse models of epilepsy

    doi: 10.1111/epi.13978

    Figure Lengend Snippet: KCNQ activation increases flurothyl-induced seizure latencies in Kcnq1 mice and decreases spontaneous seizure frequency in Kcna1 −/− mice. ( A ) Administration of RTG (10 mg·kg −1 , ip) had no significant effect on latency to flurothyl-induced myoclonic jerks (MJ) in Tac:N:NIHS-BC Kcna1 +/+ and Kcna1 −/− mice. However, RTG decreased latency to myoclonic jerk in Kcnq1 A340E/A340E mutant mice ( B ). ( C ) Administration of RTG (10 mg·kg −1 , ip) had no significant effect on latency to flurothyl-induced generalized tonic-clonic seizures (GTCS) in Tac:N:NIHS-BC Kcna1 +/+ and Kcna1 −/− mice. However, RTG significantly increased the latency to GTCS in Kcnq1 strain (C57BL/6) mice ( D ). ( E ) EEG traces showing representative spontaneous seizure activity in Kcna1 −/− mice after receiving either VEH or RTG (10 mg·kg −1 ). ( F ) Analysis of spontaneous seizure frequency in Kcna1 −/− mice (n = 6) showed a significant decrease after treatment with 10 mg·kg −1 RTG. ( G ) Average spontaneous seizure durations were similar in Kcna1 −/− mice that exhibited seizures after VEH and 10 mg·kg −1 RTG administration (n = 3). For ( A-D ): *, P ≤ 0.05; **, P ≤ 0.01 (two-tailed unpaired Student’s t -test; n = 6–11 per treatment). For ( F ) and ( G ): *, P ≤ 0.05 (two-tailed paired Student’s t -test). Abbreviations: LT, left temporal; VEH, vehicle; RTG, retigabine; NS, not significant compared to VEH control.

    Article Snippet: Retigabine dihydrochloride (RTG; Alomone Labs, Jerusalem, Israel) was administered at a concentration of 5, 10, or 20 mg·kg−1 , as specified in the text.

    Techniques: Activation Assay, Mouse Assay, Mutagenesis, Activity Assay, Two Tailed Test