orai3  (Alomone Labs)


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    Alomone Labs orai3
    Orai3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orai3/product/Alomone Labs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    orai3 - by Bioz Stars, 2022-08
    94/100 stars

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    Alomone Labs orai3 antibodies
    Orai1, Orai2, and <t>Orai3</t> contribute to SP-Induced Cytokines Generation in LAD2 Cells. LAD2 cells with shRNA-mediated knockdown of each Orai channel or non-targeted (control) cells were exposed to SP (1 µM, for 24 h). Culture supernatants were used to quantitate (A) TNF-α, (B) IL-8 and (C) CCL-3 by ELISA. Data presented are mean ± SEM of N = 3 experiments. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons at a value ***p
    Orai3 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orai3 antibodies/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai3 antibodies - by Bioz Stars, 2022-08
    94/100 stars
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    Orai1, Orai2, and Orai3 contribute to SP-Induced Cytokines Generation in LAD2 Cells. LAD2 cells with shRNA-mediated knockdown of each Orai channel or non-targeted (control) cells were exposed to SP (1 µM, for 24 h). Culture supernatants were used to quantitate (A) TNF-α, (B) IL-8 and (C) CCL-3 by ELISA. Data presented are mean ± SEM of N = 3 experiments. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons at a value ***p

    Journal: Frontiers in Immunology

    Article Title: Inhibition of Orai Channel Function Regulates Mas-Related G Protein-Coupled Receptor-Mediated Responses in Mast Cells

    doi: 10.3389/fimmu.2021.803335

    Figure Lengend Snippet: Orai1, Orai2, and Orai3 contribute to SP-Induced Cytokines Generation in LAD2 Cells. LAD2 cells with shRNA-mediated knockdown of each Orai channel or non-targeted (control) cells were exposed to SP (1 µM, for 24 h). Culture supernatants were used to quantitate (A) TNF-α, (B) IL-8 and (C) CCL-3 by ELISA. Data presented are mean ± SEM of N = 3 experiments. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons at a value ***p

    Article Snippet: Rabbit anti-Orai1, Orai2 and Orai3 antibodies from Alomone lab (Rockville, MD, USA), anti-ERK1/2, anti-phospho-ERK1/2 (Thr-202/Tyr-204), anti-phospho-Akt (Ser-473), anti-Akt, β-Actin and goat anti-rabbit IgG-HRP were obtained from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: shRNA, Enzyme-linked Immunosorbent Assay

    Lentiviral shRNA-Mediated Knockdown of Orai1, Orai2, and Orai3 Reduces SP-Induced Responses in LAD2 Cells. (A) Knockdown efficiency of each Orai channel was determined by western blotting in cells transduced with non-targeted (control) or Orai-specific shRNA and a representative blot is shown. (B) Quantification of Orai knockdown is represented as bar graph of relative intensities of Orai protein bands normalized to respective β-Actin. (C, E, G) Cells were loaded with Fura-2AM, resuspended in Ca 2+ -free buffer (1.5 ml) and measurement of intracellular Ca 2+ mobilization was initiated via the addition of SP (0.3 µM, at 100s). At 400 s, Ca 2+ concentration of the buffer was increased to 1 mM via the addition of 1.5 µl of 1 M calcium and influx was measured for additional 18 min. (D, F, H) Effects of Orai knockdown on SP-induced degranulation, as measured by β-hexosaminidase release. Data presented are mean ± SEM of N = 4 experiments. Statistical significance was determined by two-way ANOVA with Tukey’s multiple comparisons at a value ***p

    Journal: Frontiers in Immunology

    Article Title: Inhibition of Orai Channel Function Regulates Mas-Related G Protein-Coupled Receptor-Mediated Responses in Mast Cells

    doi: 10.3389/fimmu.2021.803335

    Figure Lengend Snippet: Lentiviral shRNA-Mediated Knockdown of Orai1, Orai2, and Orai3 Reduces SP-Induced Responses in LAD2 Cells. (A) Knockdown efficiency of each Orai channel was determined by western blotting in cells transduced with non-targeted (control) or Orai-specific shRNA and a representative blot is shown. (B) Quantification of Orai knockdown is represented as bar graph of relative intensities of Orai protein bands normalized to respective β-Actin. (C, E, G) Cells were loaded with Fura-2AM, resuspended in Ca 2+ -free buffer (1.5 ml) and measurement of intracellular Ca 2+ mobilization was initiated via the addition of SP (0.3 µM, at 100s). At 400 s, Ca 2+ concentration of the buffer was increased to 1 mM via the addition of 1.5 µl of 1 M calcium and influx was measured for additional 18 min. (D, F, H) Effects of Orai knockdown on SP-induced degranulation, as measured by β-hexosaminidase release. Data presented are mean ± SEM of N = 4 experiments. Statistical significance was determined by two-way ANOVA with Tukey’s multiple comparisons at a value ***p

    Article Snippet: Rabbit anti-Orai1, Orai2 and Orai3 antibodies from Alomone lab (Rockville, MD, USA), anti-ERK1/2, anti-phospho-ERK1/2 (Thr-202/Tyr-204), anti-phospho-Akt (Ser-473), anti-Akt, β-Actin and goat anti-rabbit IgG-HRP were obtained from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: shRNA, Western Blot, Transduction, Concentration Assay

    RNA interference against Orai1, but not Orai2 or Orai3, diminishes intracellular calcium mobilization triggered by thapsigargin or histamine in HUVECs. A–H , averaged cytosolic Ca 2+ concentrations were obtained from HUVECs treated with vehicle

    Journal: The Journal of Biological Chemistry

    Article Title: Stromal Interaction Molecule 1 (STIM1) and Orai1 Mediate Histamine-evoked Calcium Entry and Nuclear Factor of Activated T-cells (NFAT) Signaling in Human Umbilical Vein Endothelial Cells

    doi: 10.1074/jbc.M114.578492

    Figure Lengend Snippet: RNA interference against Orai1, but not Orai2 or Orai3, diminishes intracellular calcium mobilization triggered by thapsigargin or histamine in HUVECs. A–H , averaged cytosolic Ca 2+ concentrations were obtained from HUVECs treated with vehicle

    Article Snippet: Immunoblots were incubated with the primary antibodies indicated, including rabbit anti-STIM1 (1:1000) , mouse anti-GAPDH (1:10,000, Fitzgerald clone 6C5), rabbit anti-Orai1 (1:500, Alomone Labs), rabbit anti-Orai2 (1:500, Alomone Labs), rabbit anti-Orai3 (1:500, Alomone Labs), mouse anti-HDAC1 (1:500, Upstate clone 2E10), mouse anti-α-tubulin (1:500, ThermoFisher Scientific clone TU-01), and mouse anti-GFP-HRP (1:1000, Miltenyi Biotec).

    Techniques: