α orai1  (Alomone Labs)


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    Alomone Labs α orai1
    SOC channel proteins in mouse cortical neurons. (A) Real time PCR (RT-PCR) of DNA complementary from primary cortical neurons obtained from E-15 wild-type mouse embryo with Orai 1–3, Stim 1, 2 and Trpc 1–7 specific primers. The experiment was repeated four times with similar results. (B) Western Blot analysis of ORAI 1, ORAI 2, STIM 2, TRPC 1 and TRPC 4 levels. Protein extracts were obtained from SH-SY5Y cells and 8–9 day in vitro (DIV) cortical neurons. Primary antibodies used were <t>α-Orai1,</t> α-Orai2, α-Trpc1, α-Trpc4, α-Stim2 and α -β Actin as a control.
    α Orai1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α orai1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α orai1 - by Bioz Stars, 2022-05
    94/100 stars

    Images

    1) Product Images from "Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons"

    Article Title: Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2017.00363

    SOC channel proteins in mouse cortical neurons. (A) Real time PCR (RT-PCR) of DNA complementary from primary cortical neurons obtained from E-15 wild-type mouse embryo with Orai 1–3, Stim 1, 2 and Trpc 1–7 specific primers. The experiment was repeated four times with similar results. (B) Western Blot analysis of ORAI 1, ORAI 2, STIM 2, TRPC 1 and TRPC 4 levels. Protein extracts were obtained from SH-SY5Y cells and 8–9 day in vitro (DIV) cortical neurons. Primary antibodies used were α-Orai1, α-Orai2, α-Trpc1, α-Trpc4, α-Stim2 and α -β Actin as a control.
    Figure Legend Snippet: SOC channel proteins in mouse cortical neurons. (A) Real time PCR (RT-PCR) of DNA complementary from primary cortical neurons obtained from E-15 wild-type mouse embryo with Orai 1–3, Stim 1, 2 and Trpc 1–7 specific primers. The experiment was repeated four times with similar results. (B) Western Blot analysis of ORAI 1, ORAI 2, STIM 2, TRPC 1 and TRPC 4 levels. Protein extracts were obtained from SH-SY5Y cells and 8–9 day in vitro (DIV) cortical neurons. Primary antibodies used were α-Orai1, α-Orai2, α-Trpc1, α-Trpc4, α-Stim2 and α -β Actin as a control.

    Techniques Used: Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Western Blot, In Vitro

    2) Product Images from "The calcium channel Orai1 is required for osteoblast development: studies in a chimeric mouse with variable in vivo Runx-cre deletion of Orai-1"

    Article Title: The calcium channel Orai1 is required for osteoblast development: studies in a chimeric mouse with variable in vivo Runx-cre deletion of Orai-1

    Journal: bioRxiv

    doi: 10.1101/2022.02.14.480443

    Variable efficacy of Runx2-cre in deleting flox/flox Orai1 in osteoblasts. It is often assumed that promoter-cre constructs uniformly delete the target in all cells of an organ. Recent findings suggest that for reasons that are not clear this is sometimes not the case (see Text), we tested this by antibody labeling of Orai1 in wild type and Orai1fl/fl-Runx2cre bone. A . Rabbit antibody detects Orai1 in osteoblasts (upper left panel). Absence of antibody eliminates labeling (lower left panel). All sections are from one wild type animal. Left and right panels are of the same section. Osteoblasts are shown independently with phalloidin rhodamine (right panels). Fields are 200 µm across. B . In the wild type animal, osteoblasts are shown with the antibody at high power (fields 200 microns wide) and in phase of the same field (right). In the lower panels, the same field of a conditional KO animal. Some of the conditional KO cells (Orai1fl/fl-Runx2cre) do not label (arrows, phase and antibody label, lower panels). C . Lower power fields, 350 µm, of Orai1 labeled osteoblasts (surrounding tissue fluorescence is an artifact) in wild type bone (top) and conditional KO bone (bottom). Osteoblasts in the wild type label strongly, including surface cells of the bone (arrows, top). Some, but not all, of the osteoblasts in the conditional KO label (arrows, bottom). D . Western blot for Orai1 in cells expressing or not expressing the protein (See Fig 5 ). Thirty-five µg loads of cell protein from the isolates indicated were run on denaturing SDS-PAGE and blotted. This blot using the very specific Alomone antibody shows a trace of Orai1 even in the osteoblast KO preparation (left lane) and reduced, but not absent, Orai1 in the MSC KO (upper panel). The beta actin re-blot (lower panel) confirms similar protein loads.
    Figure Legend Snippet: Variable efficacy of Runx2-cre in deleting flox/flox Orai1 in osteoblasts. It is often assumed that promoter-cre constructs uniformly delete the target in all cells of an organ. Recent findings suggest that for reasons that are not clear this is sometimes not the case (see Text), we tested this by antibody labeling of Orai1 in wild type and Orai1fl/fl-Runx2cre bone. A . Rabbit antibody detects Orai1 in osteoblasts (upper left panel). Absence of antibody eliminates labeling (lower left panel). All sections are from one wild type animal. Left and right panels are of the same section. Osteoblasts are shown independently with phalloidin rhodamine (right panels). Fields are 200 µm across. B . In the wild type animal, osteoblasts are shown with the antibody at high power (fields 200 microns wide) and in phase of the same field (right). In the lower panels, the same field of a conditional KO animal. Some of the conditional KO cells (Orai1fl/fl-Runx2cre) do not label (arrows, phase and antibody label, lower panels). C . Lower power fields, 350 µm, of Orai1 labeled osteoblasts (surrounding tissue fluorescence is an artifact) in wild type bone (top) and conditional KO bone (bottom). Osteoblasts in the wild type label strongly, including surface cells of the bone (arrows, top). Some, but not all, of the osteoblasts in the conditional KO label (arrows, bottom). D . Western blot for Orai1 in cells expressing or not expressing the protein (See Fig 5 ). Thirty-five µg loads of cell protein from the isolates indicated were run on denaturing SDS-PAGE and blotted. This blot using the very specific Alomone antibody shows a trace of Orai1 even in the osteoblast KO preparation (left lane) and reduced, but not absent, Orai1 in the MSC KO (upper panel). The beta actin re-blot (lower panel) confirms similar protein loads.

    Techniques Used: Construct, Antibody Labeling, Labeling, Fluorescence, Western Blot, Expressing, SDS Page

    Key features of WT and conditional KO animals on static and dynamic histomorphometry. A . Bone volume/total volume (BV/TV) of vertebrae. Vertebral bone is significantly reduced (N=7, p=0.01) in the Orai1fl/fl-Runx2cre (Orai1 cKO) mice compared to controls. The greater variability in the conditional-knockout bone is thought to reflect variable cre excision; this variability is also apparent in the cortical section thickness ( Fig 2 ; see text). B . Trabecular thickness is significantly decreased in the Orai1 conditional knockout mice (N=7, p
    Figure Legend Snippet: Key features of WT and conditional KO animals on static and dynamic histomorphometry. A . Bone volume/total volume (BV/TV) of vertebrae. Vertebral bone is significantly reduced (N=7, p=0.01) in the Orai1fl/fl-Runx2cre (Orai1 cKO) mice compared to controls. The greater variability in the conditional-knockout bone is thought to reflect variable cre excision; this variability is also apparent in the cortical section thickness ( Fig 2 ; see text). B . Trabecular thickness is significantly decreased in the Orai1 conditional knockout mice (N=7, p

    Techniques Used: Mouse Assay, Knock-Out

    Cross sections of vertebral cortex from wild-type (A) and Orai1 f/f -Runx2-cre conditional KO (B) animals. Images of H E stained histologic sections from upper lumbar vertebrae (top panels) are 1 mm wide; images from microCT scans of lower lumbar vertebrae (middle and lower panels) are 1.4 mm wide, with trabecular bone deleted. A . Wild type cortex with typical smooth bone showed relatively uniform thickness. B . The Orai1fl/fl-Runx2cre (abbreviated Orai1 cKO) animals had irregularly thinned regions (arrow) but no other distinguishing features. This is in keeping with the appearance of the bone surface in the three-dimensional reconstructions (see Fig 1). C . Cortical bone thickness, though variable, is reduced on average in Orai1fl/fl-Runx2cre (Orai1 cKO) animals (p = 0.009, N=8).
    Figure Legend Snippet: Cross sections of vertebral cortex from wild-type (A) and Orai1 f/f -Runx2-cre conditional KO (B) animals. Images of H E stained histologic sections from upper lumbar vertebrae (top panels) are 1 mm wide; images from microCT scans of lower lumbar vertebrae (middle and lower panels) are 1.4 mm wide, with trabecular bone deleted. A . Wild type cortex with typical smooth bone showed relatively uniform thickness. B . The Orai1fl/fl-Runx2cre (abbreviated Orai1 cKO) animals had irregularly thinned regions (arrow) but no other distinguishing features. This is in keeping with the appearance of the bone surface in the three-dimensional reconstructions (see Fig 1). C . Cortical bone thickness, though variable, is reduced on average in Orai1fl/fl-Runx2cre (Orai1 cKO) animals (p = 0.009, N=8).

    Techniques Used: Staining

    Surface of wild type and Orai1fl/fl-Runx2cre fourth lumbar vertebrae. Bruker CTvox software-generated three-dimensional images of vertebrae reconstructed from microCT scans at 5 µm resolution. All animals were homozygous for floxed Orai1; the conditional knockouts (lower panels) are Runx2-cre positive. A . Representative vertebrae from control animals. Apart from sites of blood vessel entry, the surface of the bone is smooth, typical for mice at four months of age. B . Representative vertebrae from Orai1fl/fl-Runx2cre animals. In contrast to the control vertebrae, the bone surface appears irregular with patchy darker areas representing regions of reduced bone.
    Figure Legend Snippet: Surface of wild type and Orai1fl/fl-Runx2cre fourth lumbar vertebrae. Bruker CTvox software-generated three-dimensional images of vertebrae reconstructed from microCT scans at 5 µm resolution. All animals were homozygous for floxed Orai1; the conditional knockouts (lower panels) are Runx2-cre positive. A . Representative vertebrae from control animals. Apart from sites of blood vessel entry, the surface of the bone is smooth, typical for mice at four months of age. B . Representative vertebrae from Orai1fl/fl-Runx2cre animals. In contrast to the control vertebrae, the bone surface appears irregular with patchy darker areas representing regions of reduced bone.

    Techniques Used: Software, Generated, Mouse Assay

    Elimination of Orai1 results in profoundly reduces OB differentiation and mineralization from OB precursors. Osteoblasts isolated as described in the methods section from control or Runx2-cre floxed (Orai1 f/f -Runx2-cre) conditional knock-out animals, were incubated 24 days in osteoblast mineralization medium and analyzed by histologic staining. Each well illustrated is 2 cm across. A . Von Kossa staining for mineral. Wild type cells made mineral nodules, but there were only rare and small nodules in Orai1 knockout cell cultures. Representative culture wells for control (WT) and conditional knockout (cKO) cells are shown on the left. Staining was quantified for four samples of each genotype. Mineralized matrix production appeared significantly reduced in cultures of Orai1-deficient osteoblasts (p
    Figure Legend Snippet: Elimination of Orai1 results in profoundly reduces OB differentiation and mineralization from OB precursors. Osteoblasts isolated as described in the methods section from control or Runx2-cre floxed (Orai1 f/f -Runx2-cre) conditional knock-out animals, were incubated 24 days in osteoblast mineralization medium and analyzed by histologic staining. Each well illustrated is 2 cm across. A . Von Kossa staining for mineral. Wild type cells made mineral nodules, but there were only rare and small nodules in Orai1 knockout cell cultures. Representative culture wells for control (WT) and conditional knockout (cKO) cells are shown on the left. Staining was quantified for four samples of each genotype. Mineralized matrix production appeared significantly reduced in cultures of Orai1-deficient osteoblasts (p

    Techniques Used: Isolation, Knock-Out, Incubation, Staining

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    Alomone Labs α orai1
    SOC channel proteins in mouse cortical neurons. (A) Real time PCR (RT-PCR) of DNA complementary from primary cortical neurons obtained from E-15 wild-type mouse embryo with Orai 1–3, Stim 1, 2 and Trpc 1–7 specific primers. The experiment was repeated four times with similar results. (B) Western Blot analysis of ORAI 1, ORAI 2, STIM 2, TRPC 1 and TRPC 4 levels. Protein extracts were obtained from SH-SY5Y cells and 8–9 day in vitro (DIV) cortical neurons. Primary antibodies used were <t>α-Orai1,</t> α-Orai2, α-Trpc1, α-Trpc4, α-Stim2 and α -β Actin as a control.
    α Orai1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α orai1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α orai1 - by Bioz Stars, 2022-05
    94/100 stars
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    SOC channel proteins in mouse cortical neurons. (A) Real time PCR (RT-PCR) of DNA complementary from primary cortical neurons obtained from E-15 wild-type mouse embryo with Orai 1–3, Stim 1, 2 and Trpc 1–7 specific primers. The experiment was repeated four times with similar results. (B) Western Blot analysis of ORAI 1, ORAI 2, STIM 2, TRPC 1 and TRPC 4 levels. Protein extracts were obtained from SH-SY5Y cells and 8–9 day in vitro (DIV) cortical neurons. Primary antibodies used were α-Orai1, α-Orai2, α-Trpc1, α-Trpc4, α-Stim2 and α -β Actin as a control.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons

    doi: 10.3389/fncel.2017.00363

    Figure Lengend Snippet: SOC channel proteins in mouse cortical neurons. (A) Real time PCR (RT-PCR) of DNA complementary from primary cortical neurons obtained from E-15 wild-type mouse embryo with Orai 1–3, Stim 1, 2 and Trpc 1–7 specific primers. The experiment was repeated four times with similar results. (B) Western Blot analysis of ORAI 1, ORAI 2, STIM 2, TRPC 1 and TRPC 4 levels. Protein extracts were obtained from SH-SY5Y cells and 8–9 day in vitro (DIV) cortical neurons. Primary antibodies used were α-Orai1, α-Orai2, α-Trpc1, α-Trpc4, α-Stim2 and α -β Actin as a control.

    Article Snippet: Primary antibodies used were α-ORAI1 (1:200) rabbit polyclonal, α-ORAI2 (1:200) rabbit polyclonal, α-STIM2 (1:500) rabbit polyclonal, α-TRPC1 (1:500) rabbit polyclonal, α-TRPC4 (1:500) rabbit polyclonal, (all from Alomone Labs, Jerusalem, Israel) and α−βActin (1:5000) mouse monoclonal (Sigma-Aldrich, St. Louis, MO, USA).

    Techniques: Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Western Blot, In Vitro

    Variable efficacy of Runx2-cre in deleting flox/flox Orai1 in osteoblasts. It is often assumed that promoter-cre constructs uniformly delete the target in all cells of an organ. Recent findings suggest that for reasons that are not clear this is sometimes not the case (see Text), we tested this by antibody labeling of Orai1 in wild type and Orai1fl/fl-Runx2cre bone. A . Rabbit antibody detects Orai1 in osteoblasts (upper left panel). Absence of antibody eliminates labeling (lower left panel). All sections are from one wild type animal. Left and right panels are of the same section. Osteoblasts are shown independently with phalloidin rhodamine (right panels). Fields are 200 µm across. B . In the wild type animal, osteoblasts are shown with the antibody at high power (fields 200 microns wide) and in phase of the same field (right). In the lower panels, the same field of a conditional KO animal. Some of the conditional KO cells (Orai1fl/fl-Runx2cre) do not label (arrows, phase and antibody label, lower panels). C . Lower power fields, 350 µm, of Orai1 labeled osteoblasts (surrounding tissue fluorescence is an artifact) in wild type bone (top) and conditional KO bone (bottom). Osteoblasts in the wild type label strongly, including surface cells of the bone (arrows, top). Some, but not all, of the osteoblasts in the conditional KO label (arrows, bottom). D . Western blot for Orai1 in cells expressing or not expressing the protein (See Fig 5 ). Thirty-five µg loads of cell protein from the isolates indicated were run on denaturing SDS-PAGE and blotted. This blot using the very specific Alomone antibody shows a trace of Orai1 even in the osteoblast KO preparation (left lane) and reduced, but not absent, Orai1 in the MSC KO (upper panel). The beta actin re-blot (lower panel) confirms similar protein loads.

    Journal: bioRxiv

    Article Title: The calcium channel Orai1 is required for osteoblast development: studies in a chimeric mouse with variable in vivo Runx-cre deletion of Orai-1

    doi: 10.1101/2022.02.14.480443

    Figure Lengend Snippet: Variable efficacy of Runx2-cre in deleting flox/flox Orai1 in osteoblasts. It is often assumed that promoter-cre constructs uniformly delete the target in all cells of an organ. Recent findings suggest that for reasons that are not clear this is sometimes not the case (see Text), we tested this by antibody labeling of Orai1 in wild type and Orai1fl/fl-Runx2cre bone. A . Rabbit antibody detects Orai1 in osteoblasts (upper left panel). Absence of antibody eliminates labeling (lower left panel). All sections are from one wild type animal. Left and right panels are of the same section. Osteoblasts are shown independently with phalloidin rhodamine (right panels). Fields are 200 µm across. B . In the wild type animal, osteoblasts are shown with the antibody at high power (fields 200 microns wide) and in phase of the same field (right). In the lower panels, the same field of a conditional KO animal. Some of the conditional KO cells (Orai1fl/fl-Runx2cre) do not label (arrows, phase and antibody label, lower panels). C . Lower power fields, 350 µm, of Orai1 labeled osteoblasts (surrounding tissue fluorescence is an artifact) in wild type bone (top) and conditional KO bone (bottom). Osteoblasts in the wild type label strongly, including surface cells of the bone (arrows, top). Some, but not all, of the osteoblasts in the conditional KO label (arrows, bottom). D . Western blot for Orai1 in cells expressing or not expressing the protein (See Fig 5 ). Thirty-five µg loads of cell protein from the isolates indicated were run on denaturing SDS-PAGE and blotted. This blot using the very specific Alomone antibody shows a trace of Orai1 even in the osteoblast KO preparation (left lane) and reduced, but not absent, Orai1 in the MSC KO (upper panel). The beta actin re-blot (lower panel) confirms similar protein loads.

    Article Snippet: Orai1 labelingRabbit polyclonal-anti Orai1 was used for tissue labeling [ ]; rabbit anti-Orai1 (extracellular) antibody was from Alomone Labs (ACC-062, Jerusalem, Israel).

    Techniques: Construct, Antibody Labeling, Labeling, Fluorescence, Western Blot, Expressing, SDS Page

    Key features of WT and conditional KO animals on static and dynamic histomorphometry. A . Bone volume/total volume (BV/TV) of vertebrae. Vertebral bone is significantly reduced (N=7, p=0.01) in the Orai1fl/fl-Runx2cre (Orai1 cKO) mice compared to controls. The greater variability in the conditional-knockout bone is thought to reflect variable cre excision; this variability is also apparent in the cortical section thickness ( Fig 2 ; see text). B . Trabecular thickness is significantly decreased in the Orai1 conditional knockout mice (N=7, p

    Journal: bioRxiv

    Article Title: The calcium channel Orai1 is required for osteoblast development: studies in a chimeric mouse with variable in vivo Runx-cre deletion of Orai-1

    doi: 10.1101/2022.02.14.480443

    Figure Lengend Snippet: Key features of WT and conditional KO animals on static and dynamic histomorphometry. A . Bone volume/total volume (BV/TV) of vertebrae. Vertebral bone is significantly reduced (N=7, p=0.01) in the Orai1fl/fl-Runx2cre (Orai1 cKO) mice compared to controls. The greater variability in the conditional-knockout bone is thought to reflect variable cre excision; this variability is also apparent in the cortical section thickness ( Fig 2 ; see text). B . Trabecular thickness is significantly decreased in the Orai1 conditional knockout mice (N=7, p

    Article Snippet: Orai1 labelingRabbit polyclonal-anti Orai1 was used for tissue labeling [ ]; rabbit anti-Orai1 (extracellular) antibody was from Alomone Labs (ACC-062, Jerusalem, Israel).

    Techniques: Mouse Assay, Knock-Out

    Cross sections of vertebral cortex from wild-type (A) and Orai1 f/f -Runx2-cre conditional KO (B) animals. Images of H E stained histologic sections from upper lumbar vertebrae (top panels) are 1 mm wide; images from microCT scans of lower lumbar vertebrae (middle and lower panels) are 1.4 mm wide, with trabecular bone deleted. A . Wild type cortex with typical smooth bone showed relatively uniform thickness. B . The Orai1fl/fl-Runx2cre (abbreviated Orai1 cKO) animals had irregularly thinned regions (arrow) but no other distinguishing features. This is in keeping with the appearance of the bone surface in the three-dimensional reconstructions (see Fig 1). C . Cortical bone thickness, though variable, is reduced on average in Orai1fl/fl-Runx2cre (Orai1 cKO) animals (p = 0.009, N=8).

    Journal: bioRxiv

    Article Title: The calcium channel Orai1 is required for osteoblast development: studies in a chimeric mouse with variable in vivo Runx-cre deletion of Orai-1

    doi: 10.1101/2022.02.14.480443

    Figure Lengend Snippet: Cross sections of vertebral cortex from wild-type (A) and Orai1 f/f -Runx2-cre conditional KO (B) animals. Images of H E stained histologic sections from upper lumbar vertebrae (top panels) are 1 mm wide; images from microCT scans of lower lumbar vertebrae (middle and lower panels) are 1.4 mm wide, with trabecular bone deleted. A . Wild type cortex with typical smooth bone showed relatively uniform thickness. B . The Orai1fl/fl-Runx2cre (abbreviated Orai1 cKO) animals had irregularly thinned regions (arrow) but no other distinguishing features. This is in keeping with the appearance of the bone surface in the three-dimensional reconstructions (see Fig 1). C . Cortical bone thickness, though variable, is reduced on average in Orai1fl/fl-Runx2cre (Orai1 cKO) animals (p = 0.009, N=8).

    Article Snippet: Orai1 labelingRabbit polyclonal-anti Orai1 was used for tissue labeling [ ]; rabbit anti-Orai1 (extracellular) antibody was from Alomone Labs (ACC-062, Jerusalem, Israel).

    Techniques: Staining

    Surface of wild type and Orai1fl/fl-Runx2cre fourth lumbar vertebrae. Bruker CTvox software-generated three-dimensional images of vertebrae reconstructed from microCT scans at 5 µm resolution. All animals were homozygous for floxed Orai1; the conditional knockouts (lower panels) are Runx2-cre positive. A . Representative vertebrae from control animals. Apart from sites of blood vessel entry, the surface of the bone is smooth, typical for mice at four months of age. B . Representative vertebrae from Orai1fl/fl-Runx2cre animals. In contrast to the control vertebrae, the bone surface appears irregular with patchy darker areas representing regions of reduced bone.

    Journal: bioRxiv

    Article Title: The calcium channel Orai1 is required for osteoblast development: studies in a chimeric mouse with variable in vivo Runx-cre deletion of Orai-1

    doi: 10.1101/2022.02.14.480443

    Figure Lengend Snippet: Surface of wild type and Orai1fl/fl-Runx2cre fourth lumbar vertebrae. Bruker CTvox software-generated three-dimensional images of vertebrae reconstructed from microCT scans at 5 µm resolution. All animals were homozygous for floxed Orai1; the conditional knockouts (lower panels) are Runx2-cre positive. A . Representative vertebrae from control animals. Apart from sites of blood vessel entry, the surface of the bone is smooth, typical for mice at four months of age. B . Representative vertebrae from Orai1fl/fl-Runx2cre animals. In contrast to the control vertebrae, the bone surface appears irregular with patchy darker areas representing regions of reduced bone.

    Article Snippet: Orai1 labelingRabbit polyclonal-anti Orai1 was used for tissue labeling [ ]; rabbit anti-Orai1 (extracellular) antibody was from Alomone Labs (ACC-062, Jerusalem, Israel).

    Techniques: Software, Generated, Mouse Assay

    Elimination of Orai1 results in profoundly reduces OB differentiation and mineralization from OB precursors. Osteoblasts isolated as described in the methods section from control or Runx2-cre floxed (Orai1 f/f -Runx2-cre) conditional knock-out animals, were incubated 24 days in osteoblast mineralization medium and analyzed by histologic staining. Each well illustrated is 2 cm across. A . Von Kossa staining for mineral. Wild type cells made mineral nodules, but there were only rare and small nodules in Orai1 knockout cell cultures. Representative culture wells for control (WT) and conditional knockout (cKO) cells are shown on the left. Staining was quantified for four samples of each genotype. Mineralized matrix production appeared significantly reduced in cultures of Orai1-deficient osteoblasts (p

    Journal: bioRxiv

    Article Title: The calcium channel Orai1 is required for osteoblast development: studies in a chimeric mouse with variable in vivo Runx-cre deletion of Orai-1

    doi: 10.1101/2022.02.14.480443

    Figure Lengend Snippet: Elimination of Orai1 results in profoundly reduces OB differentiation and mineralization from OB precursors. Osteoblasts isolated as described in the methods section from control or Runx2-cre floxed (Orai1 f/f -Runx2-cre) conditional knock-out animals, were incubated 24 days in osteoblast mineralization medium and analyzed by histologic staining. Each well illustrated is 2 cm across. A . Von Kossa staining for mineral. Wild type cells made mineral nodules, but there were only rare and small nodules in Orai1 knockout cell cultures. Representative culture wells for control (WT) and conditional knockout (cKO) cells are shown on the left. Staining was quantified for four samples of each genotype. Mineralized matrix production appeared significantly reduced in cultures of Orai1-deficient osteoblasts (p

    Article Snippet: Orai1 labelingRabbit polyclonal-anti Orai1 was used for tissue labeling [ ]; rabbit anti-Orai1 (extracellular) antibody was from Alomone Labs (ACC-062, Jerusalem, Israel).

    Techniques: Isolation, Knock-Out, Incubation, Staining