orai2  (Alomone Labs)


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    Alomone Labs orai2
    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between <t>ORAI2</t> and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value
    Orai2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orai2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai2 - by Bioz Stars, 2021-12
    94/100 stars

    Images

    1) Product Images from "Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes"

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0208981

    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value
    Figure Legend Snippet: Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value

    Techniques Used: Expressing

    Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p
    Figure Legend Snippet: Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p

    Techniques Used: Expressing

    2) Product Images from "Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells"

    Article Title: Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells

    Journal: Cancers

    doi: 10.3390/cancers11010083

    Effects of DFMO on the expression of proteins involved in SOCE in colon cancer HT29 cells. HT29 cells were treated with vehicle (control) or DFMO 5 mM, and then cells were lysed and subjected to Western blotting with antibodies against TRPC1, STIM1, STIM2, ORAI1, ORAI2 and ORAI3, followed by reprobing with anti-β-actin antibody for protein loading control. Bar graphs represent specific protein expression normalized to the β-actin content. Data are from n = 3 experiments (* p
    Figure Legend Snippet: Effects of DFMO on the expression of proteins involved in SOCE in colon cancer HT29 cells. HT29 cells were treated with vehicle (control) or DFMO 5 mM, and then cells were lysed and subjected to Western blotting with antibodies against TRPC1, STIM1, STIM2, ORAI1, ORAI2 and ORAI3, followed by reprobing with anti-β-actin antibody for protein loading control. Bar graphs represent specific protein expression normalized to the β-actin content. Data are from n = 3 experiments (* p

    Techniques Used: Expressing, Western Blot

    Effects of DFMO on the expression of genes coding for SOCE molecular players in HT29 cells. mRNA expression levels of selected genes were determined using qRT-PCR of extracts from control and DFMO-treated HT29 cells. β-actin was used as a reference. Data results are mean ± SEM from DFMO-treated cells relative to untreated cells Data are from n = 7, 7, 6, 6, 6, and 5 experiments for TRPC1 , STIM1 , STIM2 , ORAI1 , ORAI2 , and ORAI3 , respectively * p
    Figure Legend Snippet: Effects of DFMO on the expression of genes coding for SOCE molecular players in HT29 cells. mRNA expression levels of selected genes were determined using qRT-PCR of extracts from control and DFMO-treated HT29 cells. β-actin was used as a reference. Data results are mean ± SEM from DFMO-treated cells relative to untreated cells Data are from n = 7, 7, 6, 6, 6, and 5 experiments for TRPC1 , STIM1 , STIM2 , ORAI1 , ORAI2 , and ORAI3 , respectively * p

    Techniques Used: Expressing, Quantitative RT-PCR

    3) Product Images from "A Reciprocal Shift in Transient Receptor Potential Channel 1 (TRPC1) and Stromal Interaction Molecule 2 (STIM2) Contributes to Ca2+ Remodeling and Cancer Hallmarks in Colorectal Carcinoma Cells"

    Article Title: A Reciprocal Shift in Transient Receptor Potential Channel 1 (TRPC1) and Stromal Interaction Molecule 2 (STIM2) Contributes to Ca2+ Remodeling and Cancer Hallmarks in Colorectal Carcinoma Cells

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.581678

    Effects of ORAI2 and ORAI3 knockdown on SOCE in colon carcinoma (HT29) cells. A, HT29 cells were transfected with scramble siRNA or siRNA for ORAI2 or ORAI3 , and levels of corresponding mRNAs were estimated by quantitative RT-PCR. B, SOCE was estimated
    Figure Legend Snippet: Effects of ORAI2 and ORAI3 knockdown on SOCE in colon carcinoma (HT29) cells. A, HT29 cells were transfected with scramble siRNA or siRNA for ORAI2 or ORAI3 , and levels of corresponding mRNAs were estimated by quantitative RT-PCR. B, SOCE was estimated

    Techniques Used: Transfection, Quantitative RT-PCR

    4) Product Images from "Orai/CRACM1 and KCa3.1 ion channels interact in the human lung mast cell plasma membrane"

    Article Title: Orai/CRACM1 and KCa3.1 ion channels interact in the human lung mast cell plasma membrane

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-015-0112-z

    Orai2 and K Ca 3.1 proteins do not co-immunoprecipitate under the conditions used to co-immunoprecipitate Orai1 and K Ca 3.1. a Western blots using either an antibody recognising the myc epitope (left) or an antibody recognising the FLAG epitope (right) of HEK293 cell lysates. Lysates expressed either myc epitope tagged Orai2, FLAG epitope-tagged K Ca 3.1, or both as indicated in the panel above. b HEK293 cell lysates expressing the proteins indicated in the panel above were immunoprecipitated with an anti-myc antibody. Immunoprecipitates were then Western blotted using either an anti-Orai2 antibody (left) or an anti-FLAG antibody (right). Control HEK293 cell lysate expressing K Ca 3.1-FLAG protein. c As ( b ) except cell lysates were immunoprecipitated with an anti-FLAG antibody and then Western blotted with an anti-Orai2 antibody (left) or an anti-FLAG antibody (right). Control HEK293 cell lysate expressing Orai2-myc protein. Blots shown are representative of 3 independent experiments
    Figure Legend Snippet: Orai2 and K Ca 3.1 proteins do not co-immunoprecipitate under the conditions used to co-immunoprecipitate Orai1 and K Ca 3.1. a Western blots using either an antibody recognising the myc epitope (left) or an antibody recognising the FLAG epitope (right) of HEK293 cell lysates. Lysates expressed either myc epitope tagged Orai2, FLAG epitope-tagged K Ca 3.1, or both as indicated in the panel above. b HEK293 cell lysates expressing the proteins indicated in the panel above were immunoprecipitated with an anti-myc antibody. Immunoprecipitates were then Western blotted using either an anti-Orai2 antibody (left) or an anti-FLAG antibody (right). Control HEK293 cell lysate expressing K Ca 3.1-FLAG protein. c As ( b ) except cell lysates were immunoprecipitated with an anti-FLAG antibody and then Western blotted with an anti-Orai2 antibody (left) or an anti-FLAG antibody (right). Control HEK293 cell lysate expressing Orai2-myc protein. Blots shown are representative of 3 independent experiments

    Techniques Used: Western Blot, FLAG-tag, Expressing, Immunoprecipitation

    Orai1 and K Ca 3.1 co-localise in the plasma membrane. a HEK293 cells, dually transfected with FLAG-tagged K Ca 3.1 and myc-tagged Orai1 and then immunostained, show co-localisation in the plasma membrane by single plane confocal microscopy (top panels). Dually transfected HEK293 show negative staining for appropriate isotype controls (bottom panels): rabbit IgG control, dual stained with anti-myc, and mouse IgG1 control dual stained with anti-FLAG. b Fluorescence intensity plot shows increased fluorescence at the plasma membrane. myc-Orai1 is shown in green and FLAG-K Ca 3.1 in red. Arrows indicate increased fluorescence where the region of interest (ROI) intersects the plasma membrane. c HEK293 cells, dually transfected with FLAG-tagged K Ca 3.1 and myc-tagged Orai2 and then immunostained, show poor co-localisation in the plasma membrane by single plane confocal microscopy (top panels). Dually transfected HEK293 show negative staining for appropriate isotype controls (bottom panels): rabbit IgG control, dual stained with anti-myc, and mouse IgG1 control dual stained with anti-FLAG. d Fluorescence intensity plot shows poor co-localisation of K Ca 3.1 and Orai2 signals. myc-Orai2 is shown in green and FLAG-K Ca 3.1 in red. Scale bars are 10 μm
    Figure Legend Snippet: Orai1 and K Ca 3.1 co-localise in the plasma membrane. a HEK293 cells, dually transfected with FLAG-tagged K Ca 3.1 and myc-tagged Orai1 and then immunostained, show co-localisation in the plasma membrane by single plane confocal microscopy (top panels). Dually transfected HEK293 show negative staining for appropriate isotype controls (bottom panels): rabbit IgG control, dual stained with anti-myc, and mouse IgG1 control dual stained with anti-FLAG. b Fluorescence intensity plot shows increased fluorescence at the plasma membrane. myc-Orai1 is shown in green and FLAG-K Ca 3.1 in red. Arrows indicate increased fluorescence where the region of interest (ROI) intersects the plasma membrane. c HEK293 cells, dually transfected with FLAG-tagged K Ca 3.1 and myc-tagged Orai2 and then immunostained, show poor co-localisation in the plasma membrane by single plane confocal microscopy (top panels). Dually transfected HEK293 show negative staining for appropriate isotype controls (bottom panels): rabbit IgG control, dual stained with anti-myc, and mouse IgG1 control dual stained with anti-FLAG. d Fluorescence intensity plot shows poor co-localisation of K Ca 3.1 and Orai2 signals. myc-Orai2 is shown in green and FLAG-K Ca 3.1 in red. Scale bars are 10 μm

    Techniques Used: Transfection, Confocal Microscopy, Negative Staining, Staining, Fluorescence

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    Alomone Labs orai2
    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between <t>ORAI2</t> and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value
    Orai2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orai2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai2 - by Bioz Stars, 2021-12
    94/100 stars
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    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value

    Journal: PLoS ONE

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

    doi: 10.1371/journal.pone.0208981

    Figure Lengend Snippet: Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value

    Article Snippet: The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16).

    Techniques: Expressing

    Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p

    Journal: PLoS ONE

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

    doi: 10.1371/journal.pone.0208981

    Figure Lengend Snippet: Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p

    Article Snippet: The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16).

    Techniques: Expressing

    Effects of ginsenoside Rb1 on SOCE complex expression in PAs derived from MCT-induced PH rats. (A), (B) and (C) show mRNA relative expression, representative western blotting and relative protein intensity of STIM2, Orai1 and Orai2. (D), (E) and (F) show mRNA relative expression, representative western blotting and relative protein intensity of TRPC1, TRPC4. Data are presented as mean ± SD ( n = 5 each for qPCR, n = 6 each for Western blotting). * p

    Journal: Pharmaceutical Biology

    Article Title: Preventive treatment with ginsenoside Rb1 ameliorates monocrotaline-induced pulmonary arterial hypertension in rats and involves store-operated calcium entry inhibition

    doi: 10.1080/13880209.2020.1831026

    Figure Lengend Snippet: Effects of ginsenoside Rb1 on SOCE complex expression in PAs derived from MCT-induced PH rats. (A), (B) and (C) show mRNA relative expression, representative western blotting and relative protein intensity of STIM2, Orai1 and Orai2. (D), (E) and (F) show mRNA relative expression, representative western blotting and relative protein intensity of TRPC1, TRPC4. Data are presented as mean ± SD ( n = 5 each for qPCR, n = 6 each for Western blotting). * p

    Article Snippet: Following blockade with 5% BSA buffer for 2 h, membranes were probed with anti-STIM2 (1:200; Alomone Labs, Jerusalem, Israel), anti-Orai1 (1:200; Alomone Labs, Jerusalem, Israel), anti-Orai2 (1:200; Alomone Labs, Jerusalem, Israel), anti-TRPC1 (1:300; Abcam, MA), anti-TRPC4 (1:200; Alomone Labs, Jerusalem, Israel), anti-β-actin (1:500; Cell Signalling Technology, MA) primary antibodies overnight and horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; Cell Signalling Technology, MA) for 1 h. The bands were visualized by enhanced chemiluminescence reagent (Thermo Fisher Scientific, CA).

    Techniques: Expressing, Derivative Assay, Western Blot, Real-time Polymerase Chain Reaction

    Expression of Orai proteins in DCs. Western blots showing the expression of (A) Orai1, (B) Orai2, and (C) Orai3 in DCs and T cells. Whole cell lysates (30 μg) were used for each sample. Whole mouse brain, T cells, and Jurkat T cells were used as positive controls. The blots were stripped and reprobed with β-actin as a loading control. *Monomer and dimer of Orai proteins.

    Journal: Journal of Leukocyte Biology

    Article Title: Store-operated Ca2+ signaling in dendritic cells occurs independently of STIM1

    doi: 10.1189/jlb.0610381

    Figure Lengend Snippet: Expression of Orai proteins in DCs. Western blots showing the expression of (A) Orai1, (B) Orai2, and (C) Orai3 in DCs and T cells. Whole cell lysates (30 μg) were used for each sample. Whole mouse brain, T cells, and Jurkat T cells were used as positive controls. The blots were stripped and reprobed with β-actin as a loading control. *Monomer and dimer of Orai proteins.

    Article Snippet: Further, we saw similar bands with a different commercial anti-Orai2 antibody (Alomone Labs; data not shown).

    Techniques: Expressing, Western Blot

    Orai2 and STIM2 are localized at the IS. Representative confocal images of STIM2 and ORAI2 immunostaining in DCs stimulated with IgG (A)- or ICAM-1 (B and C)-coated beads. Circles indicate positions of beads clustered with DCs (original scale bars, 15 μm). Cells were colabeled with F-actin [phalloidin (PL), green] to reveal actin polarization toward contact sites. STIM2 (red) and ORAI2 (red) are polarized along with F-actin to the ICAM1-coated bead, as evident by the merged (yellow) staining. Immunostaining was assessed by a blinded scoring of > 40 random conjugates from three independent experiments.

    Journal: Journal of Leukocyte Biology

    Article Title: Store-operated Ca2+ signaling in dendritic cells occurs independently of STIM1

    doi: 10.1189/jlb.0610381

    Figure Lengend Snippet: Orai2 and STIM2 are localized at the IS. Representative confocal images of STIM2 and ORAI2 immunostaining in DCs stimulated with IgG (A)- or ICAM-1 (B and C)-coated beads. Circles indicate positions of beads clustered with DCs (original scale bars, 15 μm). Cells were colabeled with F-actin [phalloidin (PL), green] to reveal actin polarization toward contact sites. STIM2 (red) and ORAI2 (red) are polarized along with F-actin to the ICAM1-coated bead, as evident by the merged (yellow) staining. Immunostaining was assessed by a blinded scoring of > 40 random conjugates from three independent experiments.

    Article Snippet: Further, we saw similar bands with a different commercial anti-Orai2 antibody (Alomone Labs; data not shown).

    Techniques: Immunostaining, Staining

    Tg induces the association of STIM2 and Orai2 but not STIM1. (A and B) Coimmunoprecipitation showing the enhanced association between STIM2 with Orai2 upon Tg treatment. Although detected in the immunoprecipitation (IP) complex, Orai1 levels are not increased by Tg treatment. (C) STIM2 and Orai2 are not present in the coimmunoprecipitation using a STIM1 antibody. Moreover, Tg treatment does not alter levels of Orai1 in this immunoprecipitation complex.

    Journal: Journal of Leukocyte Biology

    Article Title: Store-operated Ca2+ signaling in dendritic cells occurs independently of STIM1

    doi: 10.1189/jlb.0610381

    Figure Lengend Snippet: Tg induces the association of STIM2 and Orai2 but not STIM1. (A and B) Coimmunoprecipitation showing the enhanced association between STIM2 with Orai2 upon Tg treatment. Although detected in the immunoprecipitation (IP) complex, Orai1 levels are not increased by Tg treatment. (C) STIM2 and Orai2 are not present in the coimmunoprecipitation using a STIM1 antibody. Moreover, Tg treatment does not alter levels of Orai1 in this immunoprecipitation complex.

    Article Snippet: Further, we saw similar bands with a different commercial anti-Orai2 antibody (Alomone Labs; data not shown).

    Techniques: Immunoprecipitation