rabbit anti pkr1 antibody  (Alomone Labs)


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    Name:
    Anti Prokineticin Receptor 1 extracellular Antibody
    Description:
    Anti Prokineticin Receptor 1 extracellular Antibody is directed against an epitope located at the extracellular N terminal domain of the rat Prokineticin receptor 1 The epitope is specific for PROKR1 and will not cross react with the closely related PROKR2 Anti Prokineticin Receptor 1 extracellular Antibody APR 041 can be used in western blot immunohistochemistry and flow cytometry applications and recognizes PROKR1 from rat and mouse samples
    Catalog Number:
    APR-041
    Price:
    495.0
    Category:
    Primary Antibody
    Applications:
    Indirect Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs rabbit anti pkr1 antibody
    Anti Prokineticin Receptor 1 extracellular Antibody
    Anti Prokineticin Receptor 1 extracellular Antibody is directed against an epitope located at the extracellular N terminal domain of the rat Prokineticin receptor 1 The epitope is specific for PROKR1 and will not cross react with the closely related PROKR2 Anti Prokineticin Receptor 1 extracellular Antibody APR 041 can be used in western blot immunohistochemistry and flow cytometry applications and recognizes PROKR1 from rat and mouse samples
    https://www.bioz.com/result/rabbit anti pkr1 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti pkr1 antibody - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats"

    Article Title: Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats

    Journal: Asian Journal of Andrology

    doi: 10.4103/aja.aja_109_19

    Expression of PK2 and PKR1 in varicocele. ( a ) Immunohistochemical staining of PK2 and PKR1 in the rat testes. Control testes display a weak PK2 signal. The immunoreactivity of PK2 is increased in the VC group. Control testes display a weak PKR1 signal. The immunoreactivity of PKR1 is increased in the VC group. The scale bar represents 50 μm or 100 μm. ( b ) Alteration of PK2 and PKR1 mRNA in the testes was analyzed by qPCR. ( c ) The protein level of PK2 was determined by Western blot. Sample sizes are n = 3 for each group. Each treatment group was compared with the control group, ** P
    Figure Legend Snippet: Expression of PK2 and PKR1 in varicocele. ( a ) Immunohistochemical staining of PK2 and PKR1 in the rat testes. Control testes display a weak PK2 signal. The immunoreactivity of PK2 is increased in the VC group. Control testes display a weak PKR1 signal. The immunoreactivity of PKR1 is increased in the VC group. The scale bar represents 50 μm or 100 μm. ( b ) Alteration of PK2 and PKR1 mRNA in the testes was analyzed by qPCR. ( c ) The protein level of PK2 was determined by Western blot. Sample sizes are n = 3 for each group. Each treatment group was compared with the control group, ** P

    Techniques Used: Expressing, Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction, Western Blot

    PK2 expression and oxidative stress in GC-2 cells. GC-2 cells were cocultured with various concentrations of H 2 O 2 (0, 200, 400, and 600 μmol l −1 ) for 6 h. ( a ) The basal expression levels of PK2 and PKR1 in GC-2 cells were determined by immunofluorescence. The scale bars represent 100 μm. ( b ) The viability of GC-2 cells in the different groups was determined by CCK8 assay. ( c ) Alterations in the mRNA expression of PK2 in GC-2 cells in the different groups were determined by qPCR. ( d ) Alteration of the protein expression of PK2 in GC-2 cells in the different groups was analyzed by Western blot. All experiments were replicated in three independent experiments from different cell samples. Each treatment group was compared with the control group, * P
    Figure Legend Snippet: PK2 expression and oxidative stress in GC-2 cells. GC-2 cells were cocultured with various concentrations of H 2 O 2 (0, 200, 400, and 600 μmol l −1 ) for 6 h. ( a ) The basal expression levels of PK2 and PKR1 in GC-2 cells were determined by immunofluorescence. The scale bars represent 100 μm. ( b ) The viability of GC-2 cells in the different groups was determined by CCK8 assay. ( c ) Alterations in the mRNA expression of PK2 in GC-2 cells in the different groups were determined by qPCR. ( d ) Alteration of the protein expression of PK2 in GC-2 cells in the different groups was analyzed by Western blot. All experiments were replicated in three independent experiments from different cell samples. Each treatment group was compared with the control group, * P

    Techniques Used: Expressing, Immunofluorescence, CCK-8 Assay, Real-time Polymerase Chain Reaction, Western Blot

    2) Product Images from "Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats"

    Article Title: Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats

    Journal: Asian Journal of Andrology

    doi: 10.4103/aja.aja_109_19

    Expression of PK2 and PKR1 in varicocele. ( a ) Immunohistochemical staining of PK2 and PKR1 in the rat testes. Control testes display a weak PK2 signal. The immunoreactivity of PK2 is increased in the VC group. Control testes display a weak PKR1 signal. The immunoreactivity of PKR1 is increased in the VC group. The scale bar represents 50 μm or 100 μm. ( b ) Alteration of PK2 and PKR1 mRNA in the testes was analyzed by qPCR. ( c ) The protein level of PK2 was determined by Western blot. Sample sizes are n = 3 for each group. Each treatment group was compared with the control group, ** P
    Figure Legend Snippet: Expression of PK2 and PKR1 in varicocele. ( a ) Immunohistochemical staining of PK2 and PKR1 in the rat testes. Control testes display a weak PK2 signal. The immunoreactivity of PK2 is increased in the VC group. Control testes display a weak PKR1 signal. The immunoreactivity of PKR1 is increased in the VC group. The scale bar represents 50 μm or 100 μm. ( b ) Alteration of PK2 and PKR1 mRNA in the testes was analyzed by qPCR. ( c ) The protein level of PK2 was determined by Western blot. Sample sizes are n = 3 for each group. Each treatment group was compared with the control group, ** P

    Techniques Used: Expressing, Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction, Western Blot

    PK2 expression and oxidative stress in GC-2 cells. GC-2 cells were cocultured with various concentrations of H 2 O 2 (0, 200, 400, and 600 μmol l −1 ) for 6 h. ( a ) The basal expression levels of PK2 and PKR1 in GC-2 cells were determined by immunofluorescence. The scale bars represent 100 μm. ( b ) The viability of GC-2 cells in the different groups was determined by CCK8 assay. ( c ) Alterations in the mRNA expression of PK2 in GC-2 cells in the different groups were determined by qPCR. ( d ) Alteration of the protein expression of PK2 in GC-2 cells in the different groups was analyzed by Western blot. All experiments were replicated in three independent experiments from different cell samples. Each treatment group was compared with the control group, * P
    Figure Legend Snippet: PK2 expression and oxidative stress in GC-2 cells. GC-2 cells were cocultured with various concentrations of H 2 O 2 (0, 200, 400, and 600 μmol l −1 ) for 6 h. ( a ) The basal expression levels of PK2 and PKR1 in GC-2 cells were determined by immunofluorescence. The scale bars represent 100 μm. ( b ) The viability of GC-2 cells in the different groups was determined by CCK8 assay. ( c ) Alterations in the mRNA expression of PK2 in GC-2 cells in the different groups were determined by qPCR. ( d ) Alteration of the protein expression of PK2 in GC-2 cells in the different groups was analyzed by Western blot. All experiments were replicated in three independent experiments from different cell samples. Each treatment group was compared with the control group, * P

    Techniques Used: Expressing, Immunofluorescence, CCK-8 Assay, Real-time Polymerase Chain Reaction, Western Blot

    Related Articles

    Incubation:

    Article Title: Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats
    Article Snippet: .. Then, the testicular sections were blocked with 2.5% (w/v) nonfat milk for 10 min and incubated with the primary antibodies: rabbit anti-PK2 antibody (1:200, Abcam) and rabbit anti-PKR1 antibody (1:500, Alomone, Jerusalem, Israel) overnight at 4°C. ..

    Article Title: Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2/PKR1 Pathway
    Article Snippet: .. Membranes were blocked in 5% skimmed milk/PBS for 1 h at 4°C and incubated with the following primary antibodies: rabbit polyclonal anti-PK2 (cat. no. ab76747, 1 : 200, Abcam), rabbit polyclonal anti-PKR1 (cat. no. APR-041, 1 : 200, Alomone Labs), and mouse monoclonal anti-GAPDH (cat no. WB2197, 1 : 2000, Well Biotech Co., Ltd.) at 4°C overnight and then incubated with horseradish peroxidase- (HRP-) labeled goat anti-rabbit secondary antibody (cat. no. 111-035-003, 1 : 5000, Jackson ImmunoResearch Laboratories, Inc.) at 4°C for 1 h. ..

    Article Title: Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats
    Article Snippet: .. Immunofluorescence The GC-2 cells were seeded on a cover slip and fixed with cooled 4% (w/v ) formaldehyde at 4°C for 20 min. After blocking with 5% (v/v) normal goat serum (Beyotime) at room temperature for 1 h, the cells were incubated with the rabbit anti-PK2 antibody (1:200, Abcam) or rabbit anti-PKR1 antibody (1:500, Alomone) at 4°C overnight. ..

    Labeling:

    Article Title: Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2/PKR1 Pathway
    Article Snippet: .. Membranes were blocked in 5% skimmed milk/PBS for 1 h at 4°C and incubated with the following primary antibodies: rabbit polyclonal anti-PK2 (cat. no. ab76747, 1 : 200, Abcam), rabbit polyclonal anti-PKR1 (cat. no. APR-041, 1 : 200, Alomone Labs), and mouse monoclonal anti-GAPDH (cat no. WB2197, 1 : 2000, Well Biotech Co., Ltd.) at 4°C overnight and then incubated with horseradish peroxidase- (HRP-) labeled goat anti-rabbit secondary antibody (cat. no. 111-035-003, 1 : 5000, Jackson ImmunoResearch Laboratories, Inc.) at 4°C for 1 h. ..

    Immunofluorescence:

    Article Title: Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats
    Article Snippet: .. Immunofluorescence The GC-2 cells were seeded on a cover slip and fixed with cooled 4% (w/v ) formaldehyde at 4°C for 20 min. After blocking with 5% (v/v) normal goat serum (Beyotime) at room temperature for 1 h, the cells were incubated with the rabbit anti-PK2 antibody (1:200, Abcam) or rabbit anti-PKR1 antibody (1:500, Alomone) at 4°C overnight. ..

    Blocking Assay:

    Article Title: Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats
    Article Snippet: .. Immunofluorescence The GC-2 cells were seeded on a cover slip and fixed with cooled 4% (w/v ) formaldehyde at 4°C for 20 min. After blocking with 5% (v/v) normal goat serum (Beyotime) at room temperature for 1 h, the cells were incubated with the rabbit anti-PK2 antibody (1:200, Abcam) or rabbit anti-PKR1 antibody (1:500, Alomone) at 4°C overnight. ..

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  • 93
    Alomone Labs rabbit anti pkr1 antibody
    Expression of PK2 and <t>PKR1</t> in varicocele. ( a ) Immunohistochemical staining of PK2 and PKR1 in the rat testes. Control testes display a weak PK2 signal. The immunoreactivity of PK2 is increased in the VC group. Control testes display a weak PKR1 signal. The immunoreactivity of PKR1 is increased in the VC group. The scale bar represents 50 μm or 100 μm. ( b ) Alteration of PK2 and PKR1 mRNA in the testes was analyzed by qPCR. ( c ) The protein level of PK2 was determined by Western blot. Sample sizes are n = 3 for each group. Each treatment group was compared with the control group, ** P
    Rabbit Anti Pkr1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti pkr1 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti pkr1 antibody - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Expression of PK2 and PKR1 in varicocele. ( a ) Immunohistochemical staining of PK2 and PKR1 in the rat testes. Control testes display a weak PK2 signal. The immunoreactivity of PK2 is increased in the VC group. Control testes display a weak PKR1 signal. The immunoreactivity of PKR1 is increased in the VC group. The scale bar represents 50 μm or 100 μm. ( b ) Alteration of PK2 and PKR1 mRNA in the testes was analyzed by qPCR. ( c ) The protein level of PK2 was determined by Western blot. Sample sizes are n = 3 for each group. Each treatment group was compared with the control group, ** P

    Journal: Asian Journal of Andrology

    Article Title: Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats

    doi: 10.4103/aja.aja_109_19

    Figure Lengend Snippet: Expression of PK2 and PKR1 in varicocele. ( a ) Immunohistochemical staining of PK2 and PKR1 in the rat testes. Control testes display a weak PK2 signal. The immunoreactivity of PK2 is increased in the VC group. Control testes display a weak PKR1 signal. The immunoreactivity of PKR1 is increased in the VC group. The scale bar represents 50 μm or 100 μm. ( b ) Alteration of PK2 and PKR1 mRNA in the testes was analyzed by qPCR. ( c ) The protein level of PK2 was determined by Western blot. Sample sizes are n = 3 for each group. Each treatment group was compared with the control group, ** P

    Article Snippet: Then, the testicular sections were blocked with 2.5% (w/v) nonfat milk for 10 min and incubated with the primary antibodies: rabbit anti-PK2 antibody (1:200, Abcam) and rabbit anti-PKR1 antibody (1:500, Alomone, Jerusalem, Israel) overnight at 4°C.

    Techniques: Expressing, Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction, Western Blot

    PK2 expression and oxidative stress in GC-2 cells. GC-2 cells were cocultured with various concentrations of H 2 O 2 (0, 200, 400, and 600 μmol l −1 ) for 6 h. ( a ) The basal expression levels of PK2 and PKR1 in GC-2 cells were determined by immunofluorescence. The scale bars represent 100 μm. ( b ) The viability of GC-2 cells in the different groups was determined by CCK8 assay. ( c ) Alterations in the mRNA expression of PK2 in GC-2 cells in the different groups were determined by qPCR. ( d ) Alteration of the protein expression of PK2 in GC-2 cells in the different groups was analyzed by Western blot. All experiments were replicated in three independent experiments from different cell samples. Each treatment group was compared with the control group, * P

    Journal: Asian Journal of Andrology

    Article Title: Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats

    doi: 10.4103/aja.aja_109_19

    Figure Lengend Snippet: PK2 expression and oxidative stress in GC-2 cells. GC-2 cells were cocultured with various concentrations of H 2 O 2 (0, 200, 400, and 600 μmol l −1 ) for 6 h. ( a ) The basal expression levels of PK2 and PKR1 in GC-2 cells were determined by immunofluorescence. The scale bars represent 100 μm. ( b ) The viability of GC-2 cells in the different groups was determined by CCK8 assay. ( c ) Alterations in the mRNA expression of PK2 in GC-2 cells in the different groups were determined by qPCR. ( d ) Alteration of the protein expression of PK2 in GC-2 cells in the different groups was analyzed by Western blot. All experiments were replicated in three independent experiments from different cell samples. Each treatment group was compared with the control group, * P

    Article Snippet: Then, the testicular sections were blocked with 2.5% (w/v) nonfat milk for 10 min and incubated with the primary antibodies: rabbit anti-PK2 antibody (1:200, Abcam) and rabbit anti-PKR1 antibody (1:500, Alomone, Jerusalem, Israel) overnight at 4°C.

    Techniques: Expressing, Immunofluorescence, CCK-8 Assay, Real-time Polymerase Chain Reaction, Western Blot