trpa1  (Alomone Labs)


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    Structured Review

    Alomone Labs trpa1
    Effect of WTD on the activities of TRPV1 (a), <t>TRPA1</t> (b), and TRPM8 (c) ion channels in mice. (a) Effect of WTD (6.30 g/kg, p.o.) and the TRPV1 antagonist AMG9810 (30 mg/kg, i.p.) on capsaicin-induced (2 μ g/paw) nociception. (b) Effect of WTD (6.30 g/kg, p.o.) and the TRPA1 antagonist camphor (7.6 mg/kg, s.c.) on cinnamaldehyde-induced (1.3 μ g/paw) nociception. (c) Effect of WTD (6.30 g/kg, p.o.) on icilin-induced (50 mg/kg, i.p.) jumping and WDS behaviors. Data are represented as the mean ± SEM ( n = 6). *** P
    Trpa1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpa1/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpa1 - by Bioz Stars, 2022-05
    95/100 stars

    Images

    1) Product Images from "Wu-Tou Decoction Inhibits Chronic Inflammatory Pain in Mice: Participation of TRPV1 and TRPA1 Ion Channels"

    Article Title: Wu-Tou Decoction Inhibits Chronic Inflammatory Pain in Mice: Participation of TRPV1 and TRPA1 Ion Channels

    Journal: BioMed Research International

    doi: 10.1155/2015/328707

    Effect of WTD on the activities of TRPV1 (a), TRPA1 (b), and TRPM8 (c) ion channels in mice. (a) Effect of WTD (6.30 g/kg, p.o.) and the TRPV1 antagonist AMG9810 (30 mg/kg, i.p.) on capsaicin-induced (2 μ g/paw) nociception. (b) Effect of WTD (6.30 g/kg, p.o.) and the TRPA1 antagonist camphor (7.6 mg/kg, s.c.) on cinnamaldehyde-induced (1.3 μ g/paw) nociception. (c) Effect of WTD (6.30 g/kg, p.o.) on icilin-induced (50 mg/kg, i.p.) jumping and WDS behaviors. Data are represented as the mean ± SEM ( n = 6). *** P
    Figure Legend Snippet: Effect of WTD on the activities of TRPV1 (a), TRPA1 (b), and TRPM8 (c) ion channels in mice. (a) Effect of WTD (6.30 g/kg, p.o.) and the TRPV1 antagonist AMG9810 (30 mg/kg, i.p.) on capsaicin-induced (2 μ g/paw) nociception. (b) Effect of WTD (6.30 g/kg, p.o.) and the TRPA1 antagonist camphor (7.6 mg/kg, s.c.) on cinnamaldehyde-induced (1.3 μ g/paw) nociception. (c) Effect of WTD (6.30 g/kg, p.o.) on icilin-induced (50 mg/kg, i.p.) jumping and WDS behaviors. Data are represented as the mean ± SEM ( n = 6). *** P

    Techniques Used: Mouse Assay

    Effect of WTD on the expression of TRPV1, TRPA1, and TRPM8 in DRGs of inflammatory pain mice by immunohistochemical staining. Mice were orally administrated with WTD (1.58, 3.15, and 6.30 g/kg, resp.) or water daily for 15 days. (a) Localization of positive TRPV1, TRPA1, and TRPM8 neurons in DRGs of mice from control, CFA, and WTD groups, respectively. ((b) and (c)) The numbers of TRPV1- and TRPA1-positive neurons significantly increased in DRGs in CFA group, while WTD significantly reduced their expression. (c) No significant difference of the number of TRPM8-positive neurons was observed among the five groups. Data are represented as the mean ± SEM ( n = 8). ## P
    Figure Legend Snippet: Effect of WTD on the expression of TRPV1, TRPA1, and TRPM8 in DRGs of inflammatory pain mice by immunohistochemical staining. Mice were orally administrated with WTD (1.58, 3.15, and 6.30 g/kg, resp.) or water daily for 15 days. (a) Localization of positive TRPV1, TRPA1, and TRPM8 neurons in DRGs of mice from control, CFA, and WTD groups, respectively. ((b) and (c)) The numbers of TRPV1- and TRPA1-positive neurons significantly increased in DRGs in CFA group, while WTD significantly reduced their expression. (c) No significant difference of the number of TRPM8-positive neurons was observed among the five groups. Data are represented as the mean ± SEM ( n = 8). ## P

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining

    Effect of WTD on the expression of TRPV1 (a), TRPA1 (b), and TRPM8 (c) in skins of injured paw of inflammatory pain mice by western blot. Mice were orally administrated with WTD (1.58, 3.15, and 6.30 g/kg, resp.), ibuprofen (0.14 g/kg), or water daily for 15 days. The protein expression of TRPV1 and TRPA1 significantly increased in CFA group, while WTD dose-dependently decreased their expression. No significant difference of TRPM8 protein expression was observed among the five groups. Data are represented as the mean ± SEM ( n = 4). ## P
    Figure Legend Snippet: Effect of WTD on the expression of TRPV1 (a), TRPA1 (b), and TRPM8 (c) in skins of injured paw of inflammatory pain mice by western blot. Mice were orally administrated with WTD (1.58, 3.15, and 6.30 g/kg, resp.), ibuprofen (0.14 g/kg), or water daily for 15 days. The protein expression of TRPV1 and TRPA1 significantly increased in CFA group, while WTD dose-dependently decreased their expression. No significant difference of TRPM8 protein expression was observed among the five groups. Data are represented as the mean ± SEM ( n = 4). ## P

    Techniques Used: Expressing, Mouse Assay, Western Blot

    2) Product Images from "Forsythoside A exerts antipyretic effect on yeast-induced pyrexia mice via inhibiting transient receptor potential vanilloid 1 function"

    Article Title: Forsythoside A exerts antipyretic effect on yeast-induced pyrexia mice via inhibiting transient receptor potential vanilloid 1 function

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.18045

    FT-Ainhibits TRPV1 expression while increases TRPA1 and TRPM8 expression in the hypothalamus and DRG of the mice with yeast-induced pyrexia. Expressions ofTRPV1, TRPA1 and TRPM8 were evaluated in the hypothalamus (A) and DRG (B) by Western blotting. Values are Mean ± SD of 5 mice per group. # P
    Figure Legend Snippet: FT-Ainhibits TRPV1 expression while increases TRPA1 and TRPM8 expression in the hypothalamus and DRG of the mice with yeast-induced pyrexia. Expressions ofTRPV1, TRPA1 and TRPM8 were evaluated in the hypothalamus (A) and DRG (B) by Western blotting. Values are Mean ± SD of 5 mice per group. # P

    Techniques Used: Expressing, Mouse Assay, Western Blot

    3) Product Images from "Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice"

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.576209

    Hypoxia induced activation of trigeminal ganglion neurons. (A) Experimental design. TRPA1-KO mice and WT mice were exposed to mild (13% O 2 ) or severe (10% O 2 ) hypoxia for 3 min, quickly euthanized, and then the trigeminal ganglion was sampled. Room air (21% O 2 ) was used as the control. (B) Typical example from WT mice that experienced 13% hypoxia. Note that p-ERK was positive in both neurons (arrows) and satellite cells (open triangles). (C) Ratio of p-ERK and NeuN double positive cells out of the total NeuN positive population. Each column represents mean and SEM in five animals. Two-way ANOVA revealed that there was a significant difference among O 2 concentrations ( F 2, 24 = 12.67, p = 0.0002) and interaction between O 2 concentration and genotype ( F 2, 24 = 4.882, p = 0.0166). Values of p in the figure were calculated by Sidak’s multiple comparison test.
    Figure Legend Snippet: Hypoxia induced activation of trigeminal ganglion neurons. (A) Experimental design. TRPA1-KO mice and WT mice were exposed to mild (13% O 2 ) or severe (10% O 2 ) hypoxia for 3 min, quickly euthanized, and then the trigeminal ganglion was sampled. Room air (21% O 2 ) was used as the control. (B) Typical example from WT mice that experienced 13% hypoxia. Note that p-ERK was positive in both neurons (arrows) and satellite cells (open triangles). (C) Ratio of p-ERK and NeuN double positive cells out of the total NeuN positive population. Each column represents mean and SEM in five animals. Two-way ANOVA revealed that there was a significant difference among O 2 concentrations ( F 2, 24 = 12.67, p = 0.0002) and interaction between O 2 concentration and genotype ( F 2, 24 = 4.882, p = 0.0166). Values of p in the figure were calculated by Sidak’s multiple comparison test.

    Techniques Used: Activation Assay, Mouse Assay, Concentration Assay

    Effect of genetic and pharmacological inhibition of TRPA1 on respiratory chemoreflex. (A) Representative tracing of pressure signals in whole body plethysmography. Respiration of WT and KO mice was measured using flow-through type whole body plethysmography. Every gas condition was maintained for 3 min, and the data were collected during the last 20-s period. Each stimulus was separated by intervals of 20 min or more of normal room air. Plethysmographic signal that is a pressure difference between the measuring chamber and the reference chamber, and O 2 concentration in the measuring chamber were continuously monitored. Data for baseline and recovery periods were obtained during the last 3 min before the next stimulation. (B) Group data obtained in whole body plethysmography. Data are shown as mean ± SEM. n = 8 for WT and n = 8 for KO mice. Two-way ANOVA revealed that there was a significant difference among gas conditions ( F 8, 112 = 101.7, p
    Figure Legend Snippet: Effect of genetic and pharmacological inhibition of TRPA1 on respiratory chemoreflex. (A) Representative tracing of pressure signals in whole body plethysmography. Respiration of WT and KO mice was measured using flow-through type whole body plethysmography. Every gas condition was maintained for 3 min, and the data were collected during the last 20-s period. Each stimulus was separated by intervals of 20 min or more of normal room air. Plethysmographic signal that is a pressure difference between the measuring chamber and the reference chamber, and O 2 concentration in the measuring chamber were continuously monitored. Data for baseline and recovery periods were obtained during the last 3 min before the next stimulation. (B) Group data obtained in whole body plethysmography. Data are shown as mean ± SEM. n = 8 for WT and n = 8 for KO mice. Two-way ANOVA revealed that there was a significant difference among gas conditions ( F 8, 112 = 101.7, p

    Techniques Used: Inhibition, Mouse Assay, Concentration Assay

    Distribution of Transient receptor potential ankyrin 1 (TRPA1) immunoreactivity in the nasal cavity of mouse. (A) Schematic representation of the lateral view of the mouse nasal area showing three levels of the coronal sections examined. (B) Photomicrographs taken from the areas indicated by squares (a–g) in (A) of a representative mouse. Arrows indicate TRPA1-positive structures (see text). (d’) was taken from a similar region to (d) and treated with a mixture of anti-TRPA1 antibody and an excess amount of antigen peptide. Similar results were obtained in three animals. et, ethmoturbinate; ms, maxillary sinus; mt, maxilloturbinate; nt, nasoturbinate; OB, olfactory bulb; ri, root of incisor tooth; se, septum; vn, vomeronasal organ.
    Figure Legend Snippet: Distribution of Transient receptor potential ankyrin 1 (TRPA1) immunoreactivity in the nasal cavity of mouse. (A) Schematic representation of the lateral view of the mouse nasal area showing three levels of the coronal sections examined. (B) Photomicrographs taken from the areas indicated by squares (a–g) in (A) of a representative mouse. Arrows indicate TRPA1-positive structures (see text). (d’) was taken from a similar region to (d) and treated with a mixture of anti-TRPA1 antibody and an excess amount of antigen peptide. Similar results were obtained in three animals. et, ethmoturbinate; ms, maxillary sinus; mt, maxilloturbinate; nt, nasoturbinate; OB, olfactory bulb; ri, root of incisor tooth; se, septum; vn, vomeronasal organ.

    Techniques Used:

    RNA expression of TRPA1 was undetectable in the mouse carotid body. RNAs are subjected to RT-PCR from the mouse carotid body and that from dorsal root ganglia (DRG) as a positive control of TRPA1 expression. Identification of carotid body is confirmed by the detection of tyrosine hydroxylase (TH) RNA, a specific marker for carotid body in the carotid artery region. A similar result was obtained in two mice.
    Figure Legend Snippet: RNA expression of TRPA1 was undetectable in the mouse carotid body. RNAs are subjected to RT-PCR from the mouse carotid body and that from dorsal root ganglia (DRG) as a positive control of TRPA1 expression. Identification of carotid body is confirmed by the detection of tyrosine hydroxylase (TH) RNA, a specific marker for carotid body in the carotid artery region. A similar result was obtained in two mice.

    Techniques Used: RNA Expression, Reverse Transcription Polymerase Chain Reaction, Positive Control, Expressing, Marker, Mouse Assay

    4) Product Images from "Wu-Tou Decoction Inhibits Chronic Inflammatory Pain in Mice: Participation of TRPV1 and TRPA1 Ion Channels"

    Article Title: Wu-Tou Decoction Inhibits Chronic Inflammatory Pain in Mice: Participation of TRPV1 and TRPA1 Ion Channels

    Journal: BioMed Research International

    doi: 10.1155/2015/328707

    Effect of WTD on the activities of TRPV1 (a), TRPA1 (b), and TRPM8 (c) ion channels in mice. (a) Effect of WTD (6.30 g/kg, p.o.) and the TRPV1 antagonist AMG9810 (30 mg/kg, i.p.) on capsaicin-induced (2 μ g/paw) nociception. (b) Effect of WTD (6.30 g/kg, p.o.) and the TRPA1 antagonist camphor (7.6 mg/kg, s.c.) on cinnamaldehyde-induced (1.3 μ g/paw) nociception. (c) Effect of WTD (6.30 g/kg, p.o.) on icilin-induced (50 mg/kg, i.p.) jumping and WDS behaviors. Data are represented as the mean ± SEM ( n = 6). *** P
    Figure Legend Snippet: Effect of WTD on the activities of TRPV1 (a), TRPA1 (b), and TRPM8 (c) ion channels in mice. (a) Effect of WTD (6.30 g/kg, p.o.) and the TRPV1 antagonist AMG9810 (30 mg/kg, i.p.) on capsaicin-induced (2 μ g/paw) nociception. (b) Effect of WTD (6.30 g/kg, p.o.) and the TRPA1 antagonist camphor (7.6 mg/kg, s.c.) on cinnamaldehyde-induced (1.3 μ g/paw) nociception. (c) Effect of WTD (6.30 g/kg, p.o.) on icilin-induced (50 mg/kg, i.p.) jumping and WDS behaviors. Data are represented as the mean ± SEM ( n = 6). *** P

    Techniques Used: Mouse Assay

    Effect of WTD on the expression of TRPV1, TRPA1, and TRPM8 in DRGs of inflammatory pain mice by immunohistochemical staining. Mice were orally administrated with WTD (1.58, 3.15, and 6.30 g/kg, resp.) or water daily for 15 days. (a) Localization of positive TRPV1, TRPA1, and TRPM8 neurons in DRGs of mice from control, CFA, and WTD groups, respectively. ((b) and (c)) The numbers of TRPV1- and TRPA1-positive neurons significantly increased in DRGs in CFA group, while WTD significantly reduced their expression. (c) No significant difference of the number of TRPM8-positive neurons was observed among the five groups. Data are represented as the mean ± SEM ( n = 8). ## P
    Figure Legend Snippet: Effect of WTD on the expression of TRPV1, TRPA1, and TRPM8 in DRGs of inflammatory pain mice by immunohistochemical staining. Mice were orally administrated with WTD (1.58, 3.15, and 6.30 g/kg, resp.) or water daily for 15 days. (a) Localization of positive TRPV1, TRPA1, and TRPM8 neurons in DRGs of mice from control, CFA, and WTD groups, respectively. ((b) and (c)) The numbers of TRPV1- and TRPA1-positive neurons significantly increased in DRGs in CFA group, while WTD significantly reduced their expression. (c) No significant difference of the number of TRPM8-positive neurons was observed among the five groups. Data are represented as the mean ± SEM ( n = 8). ## P

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining

    Effect of WTD on the expression of TRPV1 (a), TRPA1 (b), and TRPM8 (c) in skins of injured paw of inflammatory pain mice by western blot. Mice were orally administrated with WTD (1.58, 3.15, and 6.30 g/kg, resp.), ibuprofen (0.14 g/kg), or water daily for 15 days. The protein expression of TRPV1 and TRPA1 significantly increased in CFA group, while WTD dose-dependently decreased their expression. No significant difference of TRPM8 protein expression was observed among the five groups. Data are represented as the mean ± SEM ( n = 4). ## P
    Figure Legend Snippet: Effect of WTD on the expression of TRPV1 (a), TRPA1 (b), and TRPM8 (c) in skins of injured paw of inflammatory pain mice by western blot. Mice were orally administrated with WTD (1.58, 3.15, and 6.30 g/kg, resp.), ibuprofen (0.14 g/kg), or water daily for 15 days. The protein expression of TRPV1 and TRPA1 significantly increased in CFA group, while WTD dose-dependently decreased their expression. No significant difference of TRPM8 protein expression was observed among the five groups. Data are represented as the mean ± SEM ( n = 4). ## P

    Techniques Used: Expressing, Mouse Assay, Western Blot

    5) Product Images from "TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model"

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    Journal: The Turkish Journal of Gastroenterology

    doi: 10.5152/tjg.2018.17817

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p
    Figure Legend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)
    Figure Legend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

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    Alomone Labs anti trpa1 primary antibodies
    Axonal transport of TRPV1 and <t>TRPA1.</t> (A) TRPV1 and (C) TRPA1 immunolabeling proximal to the ligation site in saline-treated, neuritis and vinblastine-treated groups. The ligation was immediately distal to the treatment site. (B, D) Mean ratio of TRPV1 immunolabeling (ligated portion / equivalent unligated portion). P = proximal, D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Insert: Methods schematic showing sciatic nerve in the thigh. Note the red box represents the approximate position of the sections. D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Arrow head = Ligation site. * p
    Anti Trpa1 Primary Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpa1 primary antibodies/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpa1 primary antibodies - by Bioz Stars, 2022-05
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    Axonal transport of TRPV1 and TRPA1. (A) TRPV1 and (C) TRPA1 immunolabeling proximal to the ligation site in saline-treated, neuritis and vinblastine-treated groups. The ligation was immediately distal to the treatment site. (B, D) Mean ratio of TRPV1 immunolabeling (ligated portion / equivalent unligated portion). P = proximal, D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Insert: Methods schematic showing sciatic nerve in the thigh. Note the red box represents the approximate position of the sections. D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Arrow head = Ligation site. * p

    Journal: Neuroscience

    Article Title: Characterizing the mechanical properties of ectopic axonal receptive fields in inflamed nerves and following axonal transport disruption

    doi: 10.1016/j.neuroscience.2019.11.042

    Figure Lengend Snippet: Axonal transport of TRPV1 and TRPA1. (A) TRPV1 and (C) TRPA1 immunolabeling proximal to the ligation site in saline-treated, neuritis and vinblastine-treated groups. The ligation was immediately distal to the treatment site. (B, D) Mean ratio of TRPV1 immunolabeling (ligated portion / equivalent unligated portion). P = proximal, D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Insert: Methods schematic showing sciatic nerve in the thigh. Note the red box represents the approximate position of the sections. D = distal, L = ligated portion of the nerve, UL = unligated portion of the nerve. Arrow head = Ligation site. * p

    Article Snippet: Sections were blocked with 4% normal goat serum (Vector Labs, Burlingame, USA) in phosphate-buffered saline (PBS) (Sigma, UK) for 1 hour at room temperature and incubated overnight at 4 °C with polyclonal rabbit anti-TRPV1 (NB100–1617; Novus Biologicals, Abingdon, United Kingdom; diluted 1:500 in 4% normal goat serum in PBS) or anti-TRPA1 primary antibodies (ACC-03, Alomone Labs, Jerusalem, Israel; diluted 1:300 in 4% normal goat serum in PBS).

    Techniques: Immunolabeling, Ligation

    TRPA1 and TRPV4 protein levels. Hippocampus lysates were immunoreacted with specific TRPA1 (a) and TRPV4 (b) antibodies. TRPA1, but not TRPV4, increased with KA injection as compared with the PBS group. TRPA1 protein levels were attenuated by electroacupuncture (EA) at EAR (auricular) as compared with the KA-induced groups. Serious results were not observed in ST36 (ST36-ST37) and sham groups. All statistic results were analyzed and plotted as bar chart in (c) and (d).

    Journal: Mediators of Inflammation

    Article Title: Auricular Electroacupuncture Reduced Inflammation-Related Epilepsy Accompanied by Altered TRPA1, pPKCα, pPKCε, and pERk1/2 Signaling Pathways in Kainic Acid-Treated Rats

    doi: 10.1155/2014/493480

    Figure Lengend Snippet: TRPA1 and TRPV4 protein levels. Hippocampus lysates were immunoreacted with specific TRPA1 (a) and TRPV4 (b) antibodies. TRPA1, but not TRPV4, increased with KA injection as compared with the PBS group. TRPA1 protein levels were attenuated by electroacupuncture (EA) at EAR (auricular) as compared with the KA-induced groups. Serious results were not observed in ST36 (ST36-ST37) and sham groups. All statistic results were analyzed and plotted as bar chart in (c) and (d).

    Article Snippet: The membrane was blocked using 5% nonfat milk in a TBS-T buffer (10 mM Tris, pH 7.5, 100 mM NaCl, and 0.1% Tween 20), incubated with anti-TRPA1 (1 : 1000, Alomone Labs, Jerusalem, Israel), TRPV4 (1 : 1000, Alomone Labs), PKCα (pSer657) (1 : 1000, Millipore, Billerica, MA, USA), PKCε (1 : 500, Novus Biologicals, Littleton, CO, USA), and pERK1/2 (pThr202, pTyr204) (1 : 500, Novus Biologicals, Littleton, CO, USA) in TBS-T containing 1% bovine serum albumin, and incubated for 1 hour at room temperature.

    Techniques: Injection

    Hypoxia induced activation of trigeminal ganglion neurons. (A) Experimental design. TRPA1-KO mice and WT mice were exposed to mild (13% O 2 ) or severe (10% O 2 ) hypoxia for 3 min, quickly euthanized, and then the trigeminal ganglion was sampled. Room air (21% O 2 ) was used as the control. (B) Typical example from WT mice that experienced 13% hypoxia. Note that p-ERK was positive in both neurons (arrows) and satellite cells (open triangles). (C) Ratio of p-ERK and NeuN double positive cells out of the total NeuN positive population. Each column represents mean and SEM in five animals. Two-way ANOVA revealed that there was a significant difference among O 2 concentrations ( F 2, 24 = 12.67, p = 0.0002) and interaction between O 2 concentration and genotype ( F 2, 24 = 4.882, p = 0.0166). Values of p in the figure were calculated by Sidak’s multiple comparison test.

    Journal: Frontiers in Physiology

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    doi: 10.3389/fphys.2020.576209

    Figure Lengend Snippet: Hypoxia induced activation of trigeminal ganglion neurons. (A) Experimental design. TRPA1-KO mice and WT mice were exposed to mild (13% O 2 ) or severe (10% O 2 ) hypoxia for 3 min, quickly euthanized, and then the trigeminal ganglion was sampled. Room air (21% O 2 ) was used as the control. (B) Typical example from WT mice that experienced 13% hypoxia. Note that p-ERK was positive in both neurons (arrows) and satellite cells (open triangles). (C) Ratio of p-ERK and NeuN double positive cells out of the total NeuN positive population. Each column represents mean and SEM in five animals. Two-way ANOVA revealed that there was a significant difference among O 2 concentrations ( F 2, 24 = 12.67, p = 0.0002) and interaction between O 2 concentration and genotype ( F 2, 24 = 4.882, p = 0.0166). Values of p in the figure were calculated by Sidak’s multiple comparison test.

    Article Snippet: Specificity of the anti-TRPA1 antibody was examined by pre-mixing with an excess amount of antigen peptide (weight ratio 1:1, which corresponds molar ratio of ~1:100, Alomone) for 60 min.

    Techniques: Activation Assay, Mouse Assay, Concentration Assay

    Effect of genetic and pharmacological inhibition of TRPA1 on respiratory chemoreflex. (A) Representative tracing of pressure signals in whole body plethysmography. Respiration of WT and KO mice was measured using flow-through type whole body plethysmography. Every gas condition was maintained for 3 min, and the data were collected during the last 20-s period. Each stimulus was separated by intervals of 20 min or more of normal room air. Plethysmographic signal that is a pressure difference between the measuring chamber and the reference chamber, and O 2 concentration in the measuring chamber were continuously monitored. Data for baseline and recovery periods were obtained during the last 3 min before the next stimulation. (B) Group data obtained in whole body plethysmography. Data are shown as mean ± SEM. n = 8 for WT and n = 8 for KO mice. Two-way ANOVA revealed that there was a significant difference among gas conditions ( F 8, 112 = 101.7, p

    Journal: Frontiers in Physiology

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    doi: 10.3389/fphys.2020.576209

    Figure Lengend Snippet: Effect of genetic and pharmacological inhibition of TRPA1 on respiratory chemoreflex. (A) Representative tracing of pressure signals in whole body plethysmography. Respiration of WT and KO mice was measured using flow-through type whole body plethysmography. Every gas condition was maintained for 3 min, and the data were collected during the last 20-s period. Each stimulus was separated by intervals of 20 min or more of normal room air. Plethysmographic signal that is a pressure difference between the measuring chamber and the reference chamber, and O 2 concentration in the measuring chamber were continuously monitored. Data for baseline and recovery periods were obtained during the last 3 min before the next stimulation. (B) Group data obtained in whole body plethysmography. Data are shown as mean ± SEM. n = 8 for WT and n = 8 for KO mice. Two-way ANOVA revealed that there was a significant difference among gas conditions ( F 8, 112 = 101.7, p

    Article Snippet: Specificity of the anti-TRPA1 antibody was examined by pre-mixing with an excess amount of antigen peptide (weight ratio 1:1, which corresponds molar ratio of ~1:100, Alomone) for 60 min.

    Techniques: Inhibition, Mouse Assay, Concentration Assay

    Distribution of Transient receptor potential ankyrin 1 (TRPA1) immunoreactivity in the nasal cavity of mouse. (A) Schematic representation of the lateral view of the mouse nasal area showing three levels of the coronal sections examined. (B) Photomicrographs taken from the areas indicated by squares (a–g) in (A) of a representative mouse. Arrows indicate TRPA1-positive structures (see text). (d’) was taken from a similar region to (d) and treated with a mixture of anti-TRPA1 antibody and an excess amount of antigen peptide. Similar results were obtained in three animals. et, ethmoturbinate; ms, maxillary sinus; mt, maxilloturbinate; nt, nasoturbinate; OB, olfactory bulb; ri, root of incisor tooth; se, septum; vn, vomeronasal organ.

    Journal: Frontiers in Physiology

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    doi: 10.3389/fphys.2020.576209

    Figure Lengend Snippet: Distribution of Transient receptor potential ankyrin 1 (TRPA1) immunoreactivity in the nasal cavity of mouse. (A) Schematic representation of the lateral view of the mouse nasal area showing three levels of the coronal sections examined. (B) Photomicrographs taken from the areas indicated by squares (a–g) in (A) of a representative mouse. Arrows indicate TRPA1-positive structures (see text). (d’) was taken from a similar region to (d) and treated with a mixture of anti-TRPA1 antibody and an excess amount of antigen peptide. Similar results were obtained in three animals. et, ethmoturbinate; ms, maxillary sinus; mt, maxilloturbinate; nt, nasoturbinate; OB, olfactory bulb; ri, root of incisor tooth; se, septum; vn, vomeronasal organ.

    Article Snippet: Specificity of the anti-TRPA1 antibody was examined by pre-mixing with an excess amount of antigen peptide (weight ratio 1:1, which corresponds molar ratio of ~1:100, Alomone) for 60 min.

    Techniques:

    RNA expression of TRPA1 was undetectable in the mouse carotid body. RNAs are subjected to RT-PCR from the mouse carotid body and that from dorsal root ganglia (DRG) as a positive control of TRPA1 expression. Identification of carotid body is confirmed by the detection of tyrosine hydroxylase (TH) RNA, a specific marker for carotid body in the carotid artery region. A similar result was obtained in two mice.

    Journal: Frontiers in Physiology

    Article Title: Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

    doi: 10.3389/fphys.2020.576209

    Figure Lengend Snippet: RNA expression of TRPA1 was undetectable in the mouse carotid body. RNAs are subjected to RT-PCR from the mouse carotid body and that from dorsal root ganglia (DRG) as a positive control of TRPA1 expression. Identification of carotid body is confirmed by the detection of tyrosine hydroxylase (TH) RNA, a specific marker for carotid body in the carotid artery region. A similar result was obtained in two mice.

    Article Snippet: Specificity of the anti-TRPA1 antibody was examined by pre-mixing with an excess amount of antigen peptide (weight ratio 1:1, which corresponds molar ratio of ~1:100, Alomone) for 60 min.

    Techniques: RNA Expression, Reverse Transcription Polymerase Chain Reaction, Positive Control, Expressing, Marker, Mouse Assay

    WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Journal: The Turkish Journal of Gastroenterology

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    doi: 10.5152/tjg.2018.17817

    Figure Lengend Snippet: WIRS induced up-regulated protein expression of TRPA1 in DRG and duodenum after 6 h WIRS Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in DRG. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in DRG (b, c); immunohistochemistry analysis of TRPA1 protein level in duodenum (d, e) Data are mean±SEM (n=6); *p

    Article Snippet: Sections were incubated for 12 hours at 4°C with rabbit anti-TRPA1 (1:400, Alomone labs, ACC-037, Jerusalem, Israel) or mouse anti-SP (1:200, R & D SYSTEMS, MAB4375, USA).

    Techniques: Expressing, Western Blot, Immunohistochemistry

    Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Journal: The Turkish Journal of Gastroenterology

    Article Title: TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model

    doi: 10.5152/tjg.2018.17817

    Figure Lengend Snippet: Detection of TRPA1and SP expression level in spinal cord Western blot analysis and quantification of protein level (relative to control group) of TRPA1 in spinal cord. GAPDH was used as a loading control (a); immunohistochemistry analysis of TRPA1 protein level in spinal cord (b, c); immunohistochemistry analysis of SP protein level in spinal cord (d, e) Data are mean±SEM (n=6); p > 0.05, NS: no significance (Independent-Samples t-test)

    Article Snippet: Sections were incubated for 12 hours at 4°C with rabbit anti-TRPA1 (1:400, Alomone labs, ACC-037, Jerusalem, Israel) or mouse anti-SP (1:200, R & D SYSTEMS, MAB4375, USA).

    Techniques: Expressing, Western Blot, Immunohistochemistry