anti trpv4  (Alomone Labs)


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    Name:
    Anti TRPV4 ATTO Fluor 550 Antibody
    Description:
    Anti TRPV4 Antibody ACC 034 is a highly specific antibody directed against an intracellular epitope of the rat protein The antibody can be used in western blot immunoprecipitation indirect flow cytometry immunohistochemistry and immunocytochemistry applications It has been designed to recognize TRPV4 from human rat and mouse samples nAnti TRPV4 ATTO Fluor 550 Antibody ACC 034 AO is directly labeled with an ATTO 550 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine Anti TRPV4 ATTO Fluor 550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers
    Catalog Number:
    ACC-034-AO
    Price:
    686.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal ATTO 550 (Orange) Conjugated Primary Antibody
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs anti trpv4
    Anti TRPV4 ATTO Fluor 550 Antibody
    Anti TRPV4 Antibody ACC 034 is a highly specific antibody directed against an intracellular epitope of the rat protein The antibody can be used in western blot immunoprecipitation indirect flow cytometry immunohistochemistry and immunocytochemistry applications It has been designed to recognize TRPV4 from human rat and mouse samples nAnti TRPV4 ATTO Fluor 550 Antibody ACC 034 AO is directly labeled with an ATTO 550 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine Anti TRPV4 ATTO Fluor 550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers
    https://www.bioz.com/result/anti trpv4/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpv4 - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "The renal TRPV4 channel is essential for adaptation to increased dietary potassium"

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    Journal: Kidney international

    doi: 10.1016/j.kint.2016.12.010

    High dietary potassium intake augments TRPV4-dependent [Ca 2+ ] i elevations in the CD cells (A) The average time course of relative changes in Fura 2 F 340 /F 380 ratio in split-opened CDs in response to 10x elevation in flow (shown with a bar on top) from apical side. CDs were isolated from WT mice kept on control (squares) and high potassium (circles) diet, as well as TRPV4 −/− mice kept on control (triangles) and high potassium (inverted triangles) diet. The insert contains representative micrographs of a typical split-opened CD after loading with Fura-2 taken with bright-field illumination (left) and 380 nm excitation (right). (B) The summary graph of the magnitudes of flow-induced Ca 2+ responses in CD cells for the groups in (A). * - significant increase versus control.
    Figure Legend Snippet: High dietary potassium intake augments TRPV4-dependent [Ca 2+ ] i elevations in the CD cells (A) The average time course of relative changes in Fura 2 F 340 /F 380 ratio in split-opened CDs in response to 10x elevation in flow (shown with a bar on top) from apical side. CDs were isolated from WT mice kept on control (squares) and high potassium (circles) diet, as well as TRPV4 −/− mice kept on control (triangles) and high potassium (inverted triangles) diet. The insert contains representative micrographs of a typical split-opened CD after loading with Fura-2 taken with bright-field illumination (left) and 380 nm excitation (right). (B) The summary graph of the magnitudes of flow-induced Ca 2+ responses in CD cells for the groups in (A). * - significant increase versus control.

    Techniques Used: Flow Cytometry, Isolation, Mouse Assay

    Renal potassium excretion is delayed in TRPV4−/− mice fed a high K + diet Summary graph visualizing K + excretion with urine in WT (light grey) and TRPV4 −/− (dark grey) mice maintained on a regular K + diet and in response to elevated potassium load (5% K + ) for 1 or 2 days. * – significant decrease vs a respective value in WT mice.
    Figure Legend Snippet: Renal potassium excretion is delayed in TRPV4−/− mice fed a high K + diet Summary graph visualizing K + excretion with urine in WT (light grey) and TRPV4 −/− (dark grey) mice maintained on a regular K + diet and in response to elevated potassium load (5% K + ) for 1 or 2 days. * – significant decrease vs a respective value in WT mice.

    Techniques Used: Mouse Assay

    TRPV4−/− mice exhibit impaired adaptation to elevated potassium intake (A) Summary graph of plasma K + levels in WT (light grey) and TRPV4 −/− (dark grey) mice kept on control and elevated K + (5%) diet for 7 days. (B) Summary graph of K + excretion with feces in WT (light grey) and TRPV4 −/− (dark grey) mice kept on control and elevated K + (5%) diet for 7 days. (C) Summary graph of plasma aldosterone concentrations in WT (light grey) and TRPV4 −/− (dark grey) on low (0.01%) and high (5%) K + diet for 7 days. * - significant increase vs WT HK diet.
    Figure Legend Snippet: TRPV4−/− mice exhibit impaired adaptation to elevated potassium intake (A) Summary graph of plasma K + levels in WT (light grey) and TRPV4 −/− (dark grey) mice kept on control and elevated K + (5%) diet for 7 days. (B) Summary graph of K + excretion with feces in WT (light grey) and TRPV4 −/− (dark grey) mice kept on control and elevated K + (5%) diet for 7 days. (C) Summary graph of plasma aldosterone concentrations in WT (light grey) and TRPV4 −/− (dark grey) on low (0.01%) and high (5%) K + diet for 7 days. * - significant increase vs WT HK diet.

    Techniques Used: Mouse Assay

    The proposed role of TRPV4 in regulation of BK-mediated K + secretion during high potassium intake.
    Figure Legend Snippet: The proposed role of TRPV4 in regulation of BK-mediated K + secretion during high potassium intake.

    Techniques Used:

    Aldosterone-MR signaling is essential for regulation of TRPV4 in the kidney by dietary K + intake (A) Representative Western blots of whole kidney lysates from C57BL/6 mice kept on regular (0.9% K + ) and high (5% K + ) potassium (HK) diet for one week in the absence and presence of spironolactone (spir, 30 30 mg/kgBW) probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). (B) Summary graph comparing renal TRPV4 expression normalized to the respective intensity of β-actin in mice on regular diet (RD), high K + diet (HK), regular diet + spironolactone (RD(s)), high K + diet + spironolactone (HK(s)), and regular diet injected with Deoxycorticosterone acetate (DOCA). (C) Representative Western blots of whole kidney lysates from C57BL/6 mice kept on regular diet and injected with DOCA for 3 consecutive days (2.4 mg/injection/animal) probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). (D) Summary graph comparing full length α-ENaC expression normalized to the respective intensity of β-actin in mice on the same conditions as described in (B). * - significant increase versus RD; # - significant decrease versus RD.
    Figure Legend Snippet: Aldosterone-MR signaling is essential for regulation of TRPV4 in the kidney by dietary K + intake (A) Representative Western blots of whole kidney lysates from C57BL/6 mice kept on regular (0.9% K + ) and high (5% K + ) potassium (HK) diet for one week in the absence and presence of spironolactone (spir, 30 30 mg/kgBW) probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). (B) Summary graph comparing renal TRPV4 expression normalized to the respective intensity of β-actin in mice on regular diet (RD), high K + diet (HK), regular diet + spironolactone (RD(s)), high K + diet + spironolactone (HK(s)), and regular diet injected with Deoxycorticosterone acetate (DOCA). (C) Representative Western blots of whole kidney lysates from C57BL/6 mice kept on regular diet and injected with DOCA for 3 consecutive days (2.4 mg/injection/animal) probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). (D) Summary graph comparing full length α-ENaC expression normalized to the respective intensity of β-actin in mice on the same conditions as described in (B). * - significant increase versus RD; # - significant decrease versus RD.

    Techniques Used: Western Blot, Mouse Assay, Expressing, Injection

    Aldosterone but not extracellular K + increases TRPV4 abundance in mpkCCD c14 cells (A) Representative Western blots from mpkCCD c14 lysates in the control, after treatment with 1 μM aldosterone or addition of 5 mM KCl from the basolateral side for 24 hours probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). Summary graphs comparing TRPV4 (B) and full length α-ENaC (C) expression normalized to the respective intensity of β-actin in mice on the same conditions as described in (A). * - significant increase versus control.
    Figure Legend Snippet: Aldosterone but not extracellular K + increases TRPV4 abundance in mpkCCD c14 cells (A) Representative Western blots from mpkCCD c14 lysates in the control, after treatment with 1 μM aldosterone or addition of 5 mM KCl from the basolateral side for 24 hours probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). Summary graphs comparing TRPV4 (B) and full length α-ENaC (C) expression normalized to the respective intensity of β-actin in mice on the same conditions as described in (A). * - significant increase versus control.

    Techniques Used: Western Blot, Expressing, Mouse Assay

    High K + dietary intake induces apical TRPV4 accumulation in native CD cells (A) A representative confocal micrograph demonstrating TRPV4 abundance and distribution (pseudocolor green) in the CD from C57BL/6 mouse kept on regular (0.9 % K + ) intake. Here and below, representative XZ and YZ plane projections (location of the cross-section is marked by arrows), showing TRPV4 distribution along the basal-apical axis, were reconstructed from Z-stacks of confocal images. Nuclear DAPI staining is shown in pseudocolor blue. Position of the apical and basal sides is shown with “a” and “b”, respectively. (B) A representative confocal micrograph visualizing TRPV4 abundance and distribution (pseudocolor green) in the CD from C57BL/6 mouse kept on a high potassium (5% K + ) diet. (C) Distribution of averaged relative fluorescent signals representing TRPV4 localization along Z-axis in CD cells from C57BL/6 mice kept on regular and high potassium intake. For each individual cell, the fluorescent signal was normalized to its corresponding maximal value. At least 6 CDs from 3 different mice were used to obtain statistics for any given treatment.
    Figure Legend Snippet: High K + dietary intake induces apical TRPV4 accumulation in native CD cells (A) A representative confocal micrograph demonstrating TRPV4 abundance and distribution (pseudocolor green) in the CD from C57BL/6 mouse kept on regular (0.9 % K + ) intake. Here and below, representative XZ and YZ plane projections (location of the cross-section is marked by arrows), showing TRPV4 distribution along the basal-apical axis, were reconstructed from Z-stacks of confocal images. Nuclear DAPI staining is shown in pseudocolor blue. Position of the apical and basal sides is shown with “a” and “b”, respectively. (B) A representative confocal micrograph visualizing TRPV4 abundance and distribution (pseudocolor green) in the CD from C57BL/6 mouse kept on a high potassium (5% K + ) diet. (C) Distribution of averaged relative fluorescent signals representing TRPV4 localization along Z-axis in CD cells from C57BL/6 mice kept on regular and high potassium intake. For each individual cell, the fluorescent signal was normalized to its corresponding maximal value. At least 6 CDs from 3 different mice were used to obtain statistics for any given treatment.

    Techniques Used: Staining, Mouse Assay

    TRPV4 −/− mice have impaired BK channel activity in the CD (A) Representative current traces of single channel BK activity in CD cells from WT (top) and TRPV4−/− mice (bottom) kept on high (5% K + ) potassium diet for 1 week. The patches were held at a test potential of V h =−V p =+100 mV. Outward K + currents are upward. Dashed lines indicate the respective current state with c denoting the closed state. (B) Pie charts representing the frequency of observing patches with active channels ( f ) for the conditions described in (A). (C) Summary graph of functional BK levels ( f N) for WT and TRPV4 −/− mice kept on high potassium diet. * – significant decrease vs WT.
    Figure Legend Snippet: TRPV4 −/− mice have impaired BK channel activity in the CD (A) Representative current traces of single channel BK activity in CD cells from WT (top) and TRPV4−/− mice (bottom) kept on high (5% K + ) potassium diet for 1 week. The patches were held at a test potential of V h =−V p =+100 mV. Outward K + currents are upward. Dashed lines indicate the respective current state with c denoting the closed state. (B) Pie charts representing the frequency of observing patches with active channels ( f ) for the conditions described in (A). (C) Summary graph of functional BK levels ( f N) for WT and TRPV4 −/− mice kept on high potassium diet. * – significant decrease vs WT.

    Techniques Used: Mouse Assay, Activity Assay, Functional Assay

    TRPV4 expression in the kidney depends on dietary potassium intake (A) Representative Western blots from whole kidney lysates of C57BL/6 mice kept on regular (0.9% K + ) and high (5% K + , HK) potassium diet for one week. The lysates were probed with anti-TRPV4 and anti-β-actin antibodies, respectively. The channel appears as a duplet of upper glycosylated and lower non-glycosylated forms. (B) Summary graph comparing TRPV4 expression from Western blots similar to that shown in (A). Intensities of the TRPV4-reporting reporting bands were normalized to the intensities of the respective actin bands. (C) Summary graph of relative TRPV4 mRNA levels in the kidney as detected by RT q-PCR in mice treated with regular (0.9% K + ) and high (5% K + ) potassium diet for one week. Mean TRPV4 cycle threshold values were normalized to the respective HPRT cycle threshold values. * - significant increase versus regular diet.
    Figure Legend Snippet: TRPV4 expression in the kidney depends on dietary potassium intake (A) Representative Western blots from whole kidney lysates of C57BL/6 mice kept on regular (0.9% K + ) and high (5% K + , HK) potassium diet for one week. The lysates were probed with anti-TRPV4 and anti-β-actin antibodies, respectively. The channel appears as a duplet of upper glycosylated and lower non-glycosylated forms. (B) Summary graph comparing TRPV4 expression from Western blots similar to that shown in (A). Intensities of the TRPV4-reporting reporting bands were normalized to the intensities of the respective actin bands. (C) Summary graph of relative TRPV4 mRNA levels in the kidney as detected by RT q-PCR in mice treated with regular (0.9% K + ) and high (5% K + ) potassium diet for one week. Mean TRPV4 cycle threshold values were normalized to the respective HPRT cycle threshold values. * - significant increase versus regular diet.

    Techniques Used: Expressing, Western Blot, Mouse Assay, Polymerase Chain Reaction

    2) Product Images from "Rhythmic Calcium Events in the Lamina Propria Network of the Urinary Bladder of Rat Pups"

    Article Title: Rhythmic Calcium Events in the Lamina Propria Network of the Urinary Bladder of Rat Pups

    Journal: Frontiers in Systems Neuroscience

    doi: 10.3389/fnsys.2017.00087

    Whole mounts of urinary bladder tissue were prepared at the urothelial-lamina junction and contained a dense network of capillaries, small blood vessels and cells from rat pups aged ≤ P21. Capillaries, blood vessels and cells were loaded with the fluorescent dye (Fluo-2, green) (A , B,E) . Tetramethylrhodamine (TRITC; red) was picked up by urothelial cells and provided contrast to Fluo-2 loaded cells (A , B) . The cellular network in the lamina propria was heterogeneous with numerous small round, stellate or spindle shaped cells (10–20 μm) with multiple processes projecting from the soma in rat pups aged P ≤ 21. Numerous cells in the lamina propria exhibited PDGFRα-immunoreactivity (IR) whereas fewer cells exhibited TRPV4-IR (C,D) . At the urothelial-lamina propria junction, a dense capillary network was in close proximity to the network of cells expressing PDGFRα- and TRPV4-IR (E) . Capillaries were isolated using their signature, long tubular shape in the PTCL analysis software and recolored red to distinguish them from lamina propria cells. 3-D images of the capillary network and the lamina propria cell network at different angles of rotation (E , i , ii) . Calibration bar in (B) represents 10 μm (A , B) , 15 μm (C , D) . Calibration bar in (E) represents 5 μm.
    Figure Legend Snippet: Whole mounts of urinary bladder tissue were prepared at the urothelial-lamina junction and contained a dense network of capillaries, small blood vessels and cells from rat pups aged ≤ P21. Capillaries, blood vessels and cells were loaded with the fluorescent dye (Fluo-2, green) (A , B,E) . Tetramethylrhodamine (TRITC; red) was picked up by urothelial cells and provided contrast to Fluo-2 loaded cells (A , B) . The cellular network in the lamina propria was heterogeneous with numerous small round, stellate or spindle shaped cells (10–20 μm) with multiple processes projecting from the soma in rat pups aged P ≤ 21. Numerous cells in the lamina propria exhibited PDGFRα-immunoreactivity (IR) whereas fewer cells exhibited TRPV4-IR (C,D) . At the urothelial-lamina propria junction, a dense capillary network was in close proximity to the network of cells expressing PDGFRα- and TRPV4-IR (E) . Capillaries were isolated using their signature, long tubular shape in the PTCL analysis software and recolored red to distinguish them from lamina propria cells. 3-D images of the capillary network and the lamina propria cell network at different angles of rotation (E , i , ii) . Calibration bar in (B) represents 10 μm (A , B) , 15 μm (C , D) . Calibration bar in (E) represents 5 μm.

    Techniques Used: Expressing, Isolation, Software

    3) Product Images from "TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis"

    Article Title: TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S256918

    Transwell assays demonstrated that TRPV4 down-regulation greatly inhibited invasion abilities of gastric cancer cells. ( A and B ) Representative images of invasive cells following transfection with shTRPV4 or shCtrl. Magnification, ×200. ( C and D ) The column diagram of the numbers of invasive cells following transfection with shTRPV4 or shCtrl. **P
    Figure Legend Snippet: Transwell assays demonstrated that TRPV4 down-regulation greatly inhibited invasion abilities of gastric cancer cells. ( A and B ) Representative images of invasive cells following transfection with shTRPV4 or shCtrl. Magnification, ×200. ( C and D ) The column diagram of the numbers of invasive cells following transfection with shTRPV4 or shCtrl. **P

    Techniques Used: Transfection

    Detection of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues by IHC staining of tissue microarrays. ( A ) Representative images of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues. (a, b) Weakly focal positive TRPV4 expression in adjacent noncancerous gastric epithelia. (c, d) Strongly positive TRPV4 expression in a case of gastric adenocarcinoma. ( B ) Scatterplots of IHC scores of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues. ( C ) Percents of tissue samples with high TRPV4 expression and low TRPV4 expression based on the cut-off value of IHC scores in gastric adenocarcinoma tissues and adjacent noncancerous tissues (***P
    Figure Legend Snippet: Detection of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues by IHC staining of tissue microarrays. ( A ) Representative images of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues. (a, b) Weakly focal positive TRPV4 expression in adjacent noncancerous gastric epithelia. (c, d) Strongly positive TRPV4 expression in a case of gastric adenocarcinoma. ( B ) Scatterplots of IHC scores of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues. ( C ) Percents of tissue samples with high TRPV4 expression and low TRPV4 expression based on the cut-off value of IHC scores in gastric adenocarcinoma tissues and adjacent noncancerous tissues (***P

    Techniques Used: Expressing, Immunohistochemistry, Staining

    The protein expression of TRPV4 in gastric cell lines and down-regulation of TPRV4 inhibited proliferation of gastric cancer cells. ( A ) The protein expression levels of TPRV4 were detected in gastric cancer cell lines (HGC-27 and MGC-803) and normal gastric mucosa cell GES-1 by Western blot. **P
    Figure Legend Snippet: The protein expression of TRPV4 in gastric cell lines and down-regulation of TPRV4 inhibited proliferation of gastric cancer cells. ( A ) The protein expression levels of TPRV4 were detected in gastric cancer cell lines (HGC-27 and MGC-803) and normal gastric mucosa cell GES-1 by Western blot. **P

    Techniques Used: Expressing, Western Blot

    Survival curves using the Kaplan–Meier method by Log rank test. ( A and B ) The patients with high TRPV4 expression had shorter overall survival and disease-free survival than those with low TRPV4 expression. ( C and D ) The patients with low E-cadherin expression had shorter overall survival and disease-free survival than those with high E-cadherin expression. ( E and F ) The patients with high vimentin expression had shorter overall survival and disease-free survival than those with low vimentin expression. ( G ) Overall survival analysis of TRPV4 gene in gastric cancer obtained from Kaplan–Meier Plotter online ( http://kmplot.com/analysis/ ). *P
    Figure Legend Snippet: Survival curves using the Kaplan–Meier method by Log rank test. ( A and B ) The patients with high TRPV4 expression had shorter overall survival and disease-free survival than those with low TRPV4 expression. ( C and D ) The patients with low E-cadherin expression had shorter overall survival and disease-free survival than those with high E-cadherin expression. ( E and F ) The patients with high vimentin expression had shorter overall survival and disease-free survival than those with low vimentin expression. ( G ) Overall survival analysis of TRPV4 gene in gastric cancer obtained from Kaplan–Meier Plotter online ( http://kmplot.com/analysis/ ). *P

    Techniques Used: Expressing

    Representation images of TRPV4, E-cadherin and vimentin expression in gastric cancer tissues by immunohistochemical staining. Magnification, ×400. ( A – C ) High TRPV4 expression in a case of poor differentiated gastric adenocarcinoma with loss expression of E-cadherin and positive vimentin expression. ( D – F ) Low TRPV4 expression in a case of signet ring cell carcinoma with high E-cadherin expression and negative Vimentin expression. Abbreviation: TRPV4, transient receptor potential vanilloid 4.
    Figure Legend Snippet: Representation images of TRPV4, E-cadherin and vimentin expression in gastric cancer tissues by immunohistochemical staining. Magnification, ×400. ( A – C ) High TRPV4 expression in a case of poor differentiated gastric adenocarcinoma with loss expression of E-cadherin and positive vimentin expression. ( D – F ) Low TRPV4 expression in a case of signet ring cell carcinoma with high E-cadherin expression and negative Vimentin expression. Abbreviation: TRPV4, transient receptor potential vanilloid 4.

    Techniques Used: Expressing, Immunohistochemistry, Staining

    Related Articles

    Incubation:

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium
    Article Snippet: .. After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS. ..

    Article Title: TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis
    Article Snippet: .. After blocking with 20% bovine serum albumin (BSA), these pretreated slides were incubated at 4°C overnight with antibodies against TRPV4 (1:100 dilution, #ACC-034-AO, Alomone labs), E-cadherin (1:50 dilution, clone NCH-38, Dako), and vimentin (1:100 dilution, clone V9, Dako), followed by incubation with the HRP-conjugated secondary antibody (Dako). ..

    Blocking Assay:

    Article Title: TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis
    Article Snippet: .. After blocking with 20% bovine serum albumin (BSA), these pretreated slides were incubated at 4°C overnight with antibodies against TRPV4 (1:100 dilution, #ACC-034-AO, Alomone labs), E-cadherin (1:50 dilution, clone NCH-38, Dako), and vimentin (1:100 dilution, clone V9, Dako), followed by incubation with the HRP-conjugated secondary antibody (Dako). ..

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  • 93
    Alomone Labs anti trpv4
    High dietary potassium intake augments <t>TRPV4-dependent</t> [Ca 2+ ] i elevations in the CD cells (A) The average time course of relative changes in Fura 2 F 340 /F 380 ratio in split-opened CDs in response to 10x elevation in flow (shown with a bar on top) from apical side. CDs were isolated from WT mice kept on control (squares) and high potassium (circles) diet, as well as TRPV4 −/− mice kept on control (triangles) and high potassium (inverted triangles) diet. The insert contains representative micrographs of a typical split-opened CD after loading with Fura-2 taken with bright-field illumination (left) and 380 nm excitation (right). (B) The summary graph of the magnitudes of flow-induced Ca 2+ responses in CD cells for the groups in (A). * - significant increase versus control.
    Anti Trpv4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpv4/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpv4 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    High dietary potassium intake augments TRPV4-dependent [Ca 2+ ] i elevations in the CD cells (A) The average time course of relative changes in Fura 2 F 340 /F 380 ratio in split-opened CDs in response to 10x elevation in flow (shown with a bar on top) from apical side. CDs were isolated from WT mice kept on control (squares) and high potassium (circles) diet, as well as TRPV4 −/− mice kept on control (triangles) and high potassium (inverted triangles) diet. The insert contains representative micrographs of a typical split-opened CD after loading with Fura-2 taken with bright-field illumination (left) and 380 nm excitation (right). (B) The summary graph of the magnitudes of flow-induced Ca 2+ responses in CD cells for the groups in (A). * - significant increase versus control.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: High dietary potassium intake augments TRPV4-dependent [Ca 2+ ] i elevations in the CD cells (A) The average time course of relative changes in Fura 2 F 340 /F 380 ratio in split-opened CDs in response to 10x elevation in flow (shown with a bar on top) from apical side. CDs were isolated from WT mice kept on control (squares) and high potassium (circles) diet, as well as TRPV4 −/− mice kept on control (triangles) and high potassium (inverted triangles) diet. The insert contains representative micrographs of a typical split-opened CD after loading with Fura-2 taken with bright-field illumination (left) and 380 nm excitation (right). (B) The summary graph of the magnitudes of flow-induced Ca 2+ responses in CD cells for the groups in (A). * - significant increase versus control.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques: Flow Cytometry, Isolation, Mouse Assay

    Renal potassium excretion is delayed in TRPV4−/− mice fed a high K + diet Summary graph visualizing K + excretion with urine in WT (light grey) and TRPV4 −/− (dark grey) mice maintained on a regular K + diet and in response to elevated potassium load (5% K + ) for 1 or 2 days. * – significant decrease vs a respective value in WT mice.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: Renal potassium excretion is delayed in TRPV4−/− mice fed a high K + diet Summary graph visualizing K + excretion with urine in WT (light grey) and TRPV4 −/− (dark grey) mice maintained on a regular K + diet and in response to elevated potassium load (5% K + ) for 1 or 2 days. * – significant decrease vs a respective value in WT mice.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques: Mouse Assay

    TRPV4−/− mice exhibit impaired adaptation to elevated potassium intake (A) Summary graph of plasma K + levels in WT (light grey) and TRPV4 −/− (dark grey) mice kept on control and elevated K + (5%) diet for 7 days. (B) Summary graph of K + excretion with feces in WT (light grey) and TRPV4 −/− (dark grey) mice kept on control and elevated K + (5%) diet for 7 days. (C) Summary graph of plasma aldosterone concentrations in WT (light grey) and TRPV4 −/− (dark grey) on low (0.01%) and high (5%) K + diet for 7 days. * - significant increase vs WT HK diet.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: TRPV4−/− mice exhibit impaired adaptation to elevated potassium intake (A) Summary graph of plasma K + levels in WT (light grey) and TRPV4 −/− (dark grey) mice kept on control and elevated K + (5%) diet for 7 days. (B) Summary graph of K + excretion with feces in WT (light grey) and TRPV4 −/− (dark grey) mice kept on control and elevated K + (5%) diet for 7 days. (C) Summary graph of plasma aldosterone concentrations in WT (light grey) and TRPV4 −/− (dark grey) on low (0.01%) and high (5%) K + diet for 7 days. * - significant increase vs WT HK diet.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques: Mouse Assay

    The proposed role of TRPV4 in regulation of BK-mediated K + secretion during high potassium intake.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: The proposed role of TRPV4 in regulation of BK-mediated K + secretion during high potassium intake.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques:

    Aldosterone-MR signaling is essential for regulation of TRPV4 in the kidney by dietary K + intake (A) Representative Western blots of whole kidney lysates from C57BL/6 mice kept on regular (0.9% K + ) and high (5% K + ) potassium (HK) diet for one week in the absence and presence of spironolactone (spir, 30 30 mg/kgBW) probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). (B) Summary graph comparing renal TRPV4 expression normalized to the respective intensity of β-actin in mice on regular diet (RD), high K + diet (HK), regular diet + spironolactone (RD(s)), high K + diet + spironolactone (HK(s)), and regular diet injected with Deoxycorticosterone acetate (DOCA). (C) Representative Western blots of whole kidney lysates from C57BL/6 mice kept on regular diet and injected with DOCA for 3 consecutive days (2.4 mg/injection/animal) probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). (D) Summary graph comparing full length α-ENaC expression normalized to the respective intensity of β-actin in mice on the same conditions as described in (B). * - significant increase versus RD; # - significant decrease versus RD.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: Aldosterone-MR signaling is essential for regulation of TRPV4 in the kidney by dietary K + intake (A) Representative Western blots of whole kidney lysates from C57BL/6 mice kept on regular (0.9% K + ) and high (5% K + ) potassium (HK) diet for one week in the absence and presence of spironolactone (spir, 30 30 mg/kgBW) probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). (B) Summary graph comparing renal TRPV4 expression normalized to the respective intensity of β-actin in mice on regular diet (RD), high K + diet (HK), regular diet + spironolactone (RD(s)), high K + diet + spironolactone (HK(s)), and regular diet injected with Deoxycorticosterone acetate (DOCA). (C) Representative Western blots of whole kidney lysates from C57BL/6 mice kept on regular diet and injected with DOCA for 3 consecutive days (2.4 mg/injection/animal) probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). (D) Summary graph comparing full length α-ENaC expression normalized to the respective intensity of β-actin in mice on the same conditions as described in (B). * - significant increase versus RD; # - significant decrease versus RD.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques: Western Blot, Mouse Assay, Expressing, Injection

    Aldosterone but not extracellular K + increases TRPV4 abundance in mpkCCD c14 cells (A) Representative Western blots from mpkCCD c14 lysates in the control, after treatment with 1 μM aldosterone or addition of 5 mM KCl from the basolateral side for 24 hours probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). Summary graphs comparing TRPV4 (B) and full length α-ENaC (C) expression normalized to the respective intensity of β-actin in mice on the same conditions as described in (A). * - significant increase versus control.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: Aldosterone but not extracellular K + increases TRPV4 abundance in mpkCCD c14 cells (A) Representative Western blots from mpkCCD c14 lysates in the control, after treatment with 1 μM aldosterone or addition of 5 mM KCl from the basolateral side for 24 hours probed with anti-TRPV4 (upper), α-ENaC (middle), and β-actin (lower). Summary graphs comparing TRPV4 (B) and full length α-ENaC (C) expression normalized to the respective intensity of β-actin in mice on the same conditions as described in (A). * - significant increase versus control.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques: Western Blot, Expressing, Mouse Assay

    High K + dietary intake induces apical TRPV4 accumulation in native CD cells (A) A representative confocal micrograph demonstrating TRPV4 abundance and distribution (pseudocolor green) in the CD from C57BL/6 mouse kept on regular (0.9 % K + ) intake. Here and below, representative XZ and YZ plane projections (location of the cross-section is marked by arrows), showing TRPV4 distribution along the basal-apical axis, were reconstructed from Z-stacks of confocal images. Nuclear DAPI staining is shown in pseudocolor blue. Position of the apical and basal sides is shown with “a” and “b”, respectively. (B) A representative confocal micrograph visualizing TRPV4 abundance and distribution (pseudocolor green) in the CD from C57BL/6 mouse kept on a high potassium (5% K + ) diet. (C) Distribution of averaged relative fluorescent signals representing TRPV4 localization along Z-axis in CD cells from C57BL/6 mice kept on regular and high potassium intake. For each individual cell, the fluorescent signal was normalized to its corresponding maximal value. At least 6 CDs from 3 different mice were used to obtain statistics for any given treatment.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: High K + dietary intake induces apical TRPV4 accumulation in native CD cells (A) A representative confocal micrograph demonstrating TRPV4 abundance and distribution (pseudocolor green) in the CD from C57BL/6 mouse kept on regular (0.9 % K + ) intake. Here and below, representative XZ and YZ plane projections (location of the cross-section is marked by arrows), showing TRPV4 distribution along the basal-apical axis, were reconstructed from Z-stacks of confocal images. Nuclear DAPI staining is shown in pseudocolor blue. Position of the apical and basal sides is shown with “a” and “b”, respectively. (B) A representative confocal micrograph visualizing TRPV4 abundance and distribution (pseudocolor green) in the CD from C57BL/6 mouse kept on a high potassium (5% K + ) diet. (C) Distribution of averaged relative fluorescent signals representing TRPV4 localization along Z-axis in CD cells from C57BL/6 mice kept on regular and high potassium intake. For each individual cell, the fluorescent signal was normalized to its corresponding maximal value. At least 6 CDs from 3 different mice were used to obtain statistics for any given treatment.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques: Staining, Mouse Assay

    TRPV4 −/− mice have impaired BK channel activity in the CD (A) Representative current traces of single channel BK activity in CD cells from WT (top) and TRPV4−/− mice (bottom) kept on high (5% K + ) potassium diet for 1 week. The patches were held at a test potential of V h =−V p =+100 mV. Outward K + currents are upward. Dashed lines indicate the respective current state with c denoting the closed state. (B) Pie charts representing the frequency of observing patches with active channels ( f ) for the conditions described in (A). (C) Summary graph of functional BK levels ( f N) for WT and TRPV4 −/− mice kept on high potassium diet. * – significant decrease vs WT.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: TRPV4 −/− mice have impaired BK channel activity in the CD (A) Representative current traces of single channel BK activity in CD cells from WT (top) and TRPV4−/− mice (bottom) kept on high (5% K + ) potassium diet for 1 week. The patches were held at a test potential of V h =−V p =+100 mV. Outward K + currents are upward. Dashed lines indicate the respective current state with c denoting the closed state. (B) Pie charts representing the frequency of observing patches with active channels ( f ) for the conditions described in (A). (C) Summary graph of functional BK levels ( f N) for WT and TRPV4 −/− mice kept on high potassium diet. * – significant decrease vs WT.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques: Mouse Assay, Activity Assay, Functional Assay

    TRPV4 expression in the kidney depends on dietary potassium intake (A) Representative Western blots from whole kidney lysates of C57BL/6 mice kept on regular (0.9% K + ) and high (5% K + , HK) potassium diet for one week. The lysates were probed with anti-TRPV4 and anti-β-actin antibodies, respectively. The channel appears as a duplet of upper glycosylated and lower non-glycosylated forms. (B) Summary graph comparing TRPV4 expression from Western blots similar to that shown in (A). Intensities of the TRPV4-reporting reporting bands were normalized to the intensities of the respective actin bands. (C) Summary graph of relative TRPV4 mRNA levels in the kidney as detected by RT q-PCR in mice treated with regular (0.9% K + ) and high (5% K + ) potassium diet for one week. Mean TRPV4 cycle threshold values were normalized to the respective HPRT cycle threshold values. * - significant increase versus regular diet.

    Journal: Kidney international

    Article Title: The renal TRPV4 channel is essential for adaptation to increased dietary potassium

    doi: 10.1016/j.kint.2016.12.010

    Figure Lengend Snippet: TRPV4 expression in the kidney depends on dietary potassium intake (A) Representative Western blots from whole kidney lysates of C57BL/6 mice kept on regular (0.9% K + ) and high (5% K + , HK) potassium diet for one week. The lysates were probed with anti-TRPV4 and anti-β-actin antibodies, respectively. The channel appears as a duplet of upper glycosylated and lower non-glycosylated forms. (B) Summary graph comparing TRPV4 expression from Western blots similar to that shown in (A). Intensities of the TRPV4-reporting reporting bands were normalized to the intensities of the respective actin bands. (C) Summary graph of relative TRPV4 mRNA levels in the kidney as detected by RT q-PCR in mice treated with regular (0.9% K + ) and high (5% K + ) potassium diet for one week. Mean TRPV4 cycle threshold values were normalized to the respective HPRT cycle threshold values. * - significant increase versus regular diet.

    Article Snippet: After washing with PBS (3 times for 5 min) the samples were incubated for 3 hr at room temperature in dark with anti-TRPV4 tagged with ATTO 550 (1:50, Alomone Labs, Israel; Cat. # ACC-034-AO) in 2% serum in PBS.

    Techniques: Expressing, Western Blot, Mouse Assay, Polymerase Chain Reaction

    Whole mounts of urinary bladder tissue were prepared at the urothelial-lamina junction and contained a dense network of capillaries, small blood vessels and cells from rat pups aged ≤ P21. Capillaries, blood vessels and cells were loaded with the fluorescent dye (Fluo-2, green) (A , B,E) . Tetramethylrhodamine (TRITC; red) was picked up by urothelial cells and provided contrast to Fluo-2 loaded cells (A , B) . The cellular network in the lamina propria was heterogeneous with numerous small round, stellate or spindle shaped cells (10–20 μm) with multiple processes projecting from the soma in rat pups aged P ≤ 21. Numerous cells in the lamina propria exhibited PDGFRα-immunoreactivity (IR) whereas fewer cells exhibited TRPV4-IR (C,D) . At the urothelial-lamina propria junction, a dense capillary network was in close proximity to the network of cells expressing PDGFRα- and TRPV4-IR (E) . Capillaries were isolated using their signature, long tubular shape in the PTCL analysis software and recolored red to distinguish them from lamina propria cells. 3-D images of the capillary network and the lamina propria cell network at different angles of rotation (E , i , ii) . Calibration bar in (B) represents 10 μm (A , B) , 15 μm (C , D) . Calibration bar in (E) represents 5 μm.

    Journal: Frontiers in Systems Neuroscience

    Article Title: Rhythmic Calcium Events in the Lamina Propria Network of the Urinary Bladder of Rat Pups

    doi: 10.3389/fnsys.2017.00087

    Figure Lengend Snippet: Whole mounts of urinary bladder tissue were prepared at the urothelial-lamina junction and contained a dense network of capillaries, small blood vessels and cells from rat pups aged ≤ P21. Capillaries, blood vessels and cells were loaded with the fluorescent dye (Fluo-2, green) (A , B,E) . Tetramethylrhodamine (TRITC; red) was picked up by urothelial cells and provided contrast to Fluo-2 loaded cells (A , B) . The cellular network in the lamina propria was heterogeneous with numerous small round, stellate or spindle shaped cells (10–20 μm) with multiple processes projecting from the soma in rat pups aged P ≤ 21. Numerous cells in the lamina propria exhibited PDGFRα-immunoreactivity (IR) whereas fewer cells exhibited TRPV4-IR (C,D) . At the urothelial-lamina propria junction, a dense capillary network was in close proximity to the network of cells expressing PDGFRα- and TRPV4-IR (E) . Capillaries were isolated using their signature, long tubular shape in the PTCL analysis software and recolored red to distinguish them from lamina propria cells. 3-D images of the capillary network and the lamina propria cell network at different angles of rotation (E , i , ii) . Calibration bar in (B) represents 10 μm (A , B) , 15 μm (C , D) . Calibration bar in (E) represents 5 μm.

    Article Snippet: The following primary antibodies were used: rabbit anti-TRPV4 (1:1K; Abcam, catalog #ab39260) (Merrill et al., ), rabbit anti-TRPV4-ATTO-550 (1:500; Alomone Labs, Jerusalem, Israel, catalog #ACC-034-AO), rabbit anti-PDGFRα (1:8K; Thermo-Fisher Scientific, Waltham, MA, catalog #701142), rabbit anti-PDGFRα (1:1K; MyBioSource, Inc. San Diego, CA, catalog #MBS821212).

    Techniques: Expressing, Isolation, Software

    Transwell assays demonstrated that TRPV4 down-regulation greatly inhibited invasion abilities of gastric cancer cells. ( A and B ) Representative images of invasive cells following transfection with shTRPV4 or shCtrl. Magnification, ×200. ( C and D ) The column diagram of the numbers of invasive cells following transfection with shTRPV4 or shCtrl. **P

    Journal: OncoTargets and therapy

    Article Title: TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis

    doi: 10.2147/OTT.S256918

    Figure Lengend Snippet: Transwell assays demonstrated that TRPV4 down-regulation greatly inhibited invasion abilities of gastric cancer cells. ( A and B ) Representative images of invasive cells following transfection with shTRPV4 or shCtrl. Magnification, ×200. ( C and D ) The column diagram of the numbers of invasive cells following transfection with shTRPV4 or shCtrl. **P

    Article Snippet: After blocking with 20% bovine serum albumin (BSA), these pretreated slides were incubated at 4°C overnight with antibodies against TRPV4 (1:100 dilution, #ACC-034-AO, Alomone labs), E-cadherin (1:50 dilution, clone NCH-38, Dako), and vimentin (1:100 dilution, clone V9, Dako), followed by incubation with the HRP-conjugated secondary antibody (Dako).

    Techniques: Transfection

    Detection of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues by IHC staining of tissue microarrays. ( A ) Representative images of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues. (a, b) Weakly focal positive TRPV4 expression in adjacent noncancerous gastric epithelia. (c, d) Strongly positive TRPV4 expression in a case of gastric adenocarcinoma. ( B ) Scatterplots of IHC scores of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues. ( C ) Percents of tissue samples with high TRPV4 expression and low TRPV4 expression based on the cut-off value of IHC scores in gastric adenocarcinoma tissues and adjacent noncancerous tissues (***P

    Journal: OncoTargets and therapy

    Article Title: TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis

    doi: 10.2147/OTT.S256918

    Figure Lengend Snippet: Detection of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues by IHC staining of tissue microarrays. ( A ) Representative images of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues. (a, b) Weakly focal positive TRPV4 expression in adjacent noncancerous gastric epithelia. (c, d) Strongly positive TRPV4 expression in a case of gastric adenocarcinoma. ( B ) Scatterplots of IHC scores of TRPV4 expression in gastric adenocarcinoma tissues and adjacent noncancerous tissues. ( C ) Percents of tissue samples with high TRPV4 expression and low TRPV4 expression based on the cut-off value of IHC scores in gastric adenocarcinoma tissues and adjacent noncancerous tissues (***P

    Article Snippet: After blocking with 20% bovine serum albumin (BSA), these pretreated slides were incubated at 4°C overnight with antibodies against TRPV4 (1:100 dilution, #ACC-034-AO, Alomone labs), E-cadherin (1:50 dilution, clone NCH-38, Dako), and vimentin (1:100 dilution, clone V9, Dako), followed by incubation with the HRP-conjugated secondary antibody (Dako).

    Techniques: Expressing, Immunohistochemistry, Staining

    The protein expression of TRPV4 in gastric cell lines and down-regulation of TPRV4 inhibited proliferation of gastric cancer cells. ( A ) The protein expression levels of TPRV4 were detected in gastric cancer cell lines (HGC-27 and MGC-803) and normal gastric mucosa cell GES-1 by Western blot. **P

    Journal: OncoTargets and therapy

    Article Title: TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis

    doi: 10.2147/OTT.S256918

    Figure Lengend Snippet: The protein expression of TRPV4 in gastric cell lines and down-regulation of TPRV4 inhibited proliferation of gastric cancer cells. ( A ) The protein expression levels of TPRV4 were detected in gastric cancer cell lines (HGC-27 and MGC-803) and normal gastric mucosa cell GES-1 by Western blot. **P

    Article Snippet: After blocking with 20% bovine serum albumin (BSA), these pretreated slides were incubated at 4°C overnight with antibodies against TRPV4 (1:100 dilution, #ACC-034-AO, Alomone labs), E-cadherin (1:50 dilution, clone NCH-38, Dako), and vimentin (1:100 dilution, clone V9, Dako), followed by incubation with the HRP-conjugated secondary antibody (Dako).

    Techniques: Expressing, Western Blot

    Survival curves using the Kaplan–Meier method by Log rank test. ( A and B ) The patients with high TRPV4 expression had shorter overall survival and disease-free survival than those with low TRPV4 expression. ( C and D ) The patients with low E-cadherin expression had shorter overall survival and disease-free survival than those with high E-cadherin expression. ( E and F ) The patients with high vimentin expression had shorter overall survival and disease-free survival than those with low vimentin expression. ( G ) Overall survival analysis of TRPV4 gene in gastric cancer obtained from Kaplan–Meier Plotter online ( http://kmplot.com/analysis/ ). *P

    Journal: OncoTargets and therapy

    Article Title: TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis

    doi: 10.2147/OTT.S256918

    Figure Lengend Snippet: Survival curves using the Kaplan–Meier method by Log rank test. ( A and B ) The patients with high TRPV4 expression had shorter overall survival and disease-free survival than those with low TRPV4 expression. ( C and D ) The patients with low E-cadherin expression had shorter overall survival and disease-free survival than those with high E-cadherin expression. ( E and F ) The patients with high vimentin expression had shorter overall survival and disease-free survival than those with low vimentin expression. ( G ) Overall survival analysis of TRPV4 gene in gastric cancer obtained from Kaplan–Meier Plotter online ( http://kmplot.com/analysis/ ). *P

    Article Snippet: After blocking with 20% bovine serum albumin (BSA), these pretreated slides were incubated at 4°C overnight with antibodies against TRPV4 (1:100 dilution, #ACC-034-AO, Alomone labs), E-cadherin (1:50 dilution, clone NCH-38, Dako), and vimentin (1:100 dilution, clone V9, Dako), followed by incubation with the HRP-conjugated secondary antibody (Dako).

    Techniques: Expressing

    Representation images of TRPV4, E-cadherin and vimentin expression in gastric cancer tissues by immunohistochemical staining. Magnification, ×400. ( A – C ) High TRPV4 expression in a case of poor differentiated gastric adenocarcinoma with loss expression of E-cadherin and positive vimentin expression. ( D – F ) Low TRPV4 expression in a case of signet ring cell carcinoma with high E-cadherin expression and negative Vimentin expression. Abbreviation: TRPV4, transient receptor potential vanilloid 4.

    Journal: OncoTargets and therapy

    Article Title: TRPV4 Overexpression Promotes Metastasis Through Epithelial–Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis

    doi: 10.2147/OTT.S256918

    Figure Lengend Snippet: Representation images of TRPV4, E-cadherin and vimentin expression in gastric cancer tissues by immunohistochemical staining. Magnification, ×400. ( A – C ) High TRPV4 expression in a case of poor differentiated gastric adenocarcinoma with loss expression of E-cadherin and positive vimentin expression. ( D – F ) Low TRPV4 expression in a case of signet ring cell carcinoma with high E-cadherin expression and negative Vimentin expression. Abbreviation: TRPV4, transient receptor potential vanilloid 4.

    Article Snippet: After blocking with 20% bovine serum albumin (BSA), these pretreated slides were incubated at 4°C overnight with antibodies against TRPV4 (1:100 dilution, #ACC-034-AO, Alomone labs), E-cadherin (1:50 dilution, clone NCH-38, Dako), and vimentin (1:100 dilution, clone V9, Dako), followed by incubation with the HRP-conjugated secondary antibody (Dako).

    Techniques: Expressing, Immunohistochemistry, Staining