Journal: Journal of Cellular and Molecular Medicine
Article Title: The effect of Sirt1 deficiency on Ca2+ and Na+ regulation in mouse ventricular myocytes, et al. The effect of Sirt1 deficiency on Ca2+ and Na+ regulation in mouse ventricular myocytes
doi: 10.1111/jcmm.15327
Figure Lengend Snippet: Ca 2+ regulatory proteins in control and Sirt1 −/− ventricular myocytes. A, Representative immunoblot and mean data for Cav1.2, SERCA2a, NCX and CaMKII in control and Sirt1 −/− mice ventricular myocytes. B, Normalized densitometry for the protein levels (α‐Tubulin was used as an internal control; Control N = 6 and Sirt1 −/− N = 6; * P
Article Snippet: The cardiac protein extracts were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and then transferred to polyvinylidene difluoride membranes (Millipore) that were incubated with the listed antibodies: Cav1.2 (1:1000, AACC‐033, rabbit polyclonal antibody; Alomone Labs, Jerusalem, Israel), CaMKII (1:1000, sc‐5306, mouse monoclonal antibody; Santa Cruz Biotechnology, Dallas, USA), NCX (1:1000, mouse monoclonal antibody, ab2869; Abcam), SERCA2a (1:5000, sc‐376235, mouse monoclonal antibody; Santa Cruz Biotechnology, Dallas, USA) and α‐tubulin (1:10 000, sc‐5286, mouse monoclonal antibody; Santa Cruz Biotechnology, Dallas, USA).
Techniques: Mouse Assay