trpv3  (Alomone Labs)


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    Name:
    Anti TRPV3 extracellular Antibody
    Description:
    Anti TRPV3 extracellular Antibody ACC 033 is a highly specific antibody directed against an extracellular epitope of human TRPV3 The antibody can be used in western blot immunohistochemical and immunocytochemical applications As the antibody recognizes an extracellular epitope it can be used as a tool to detect TRPV3 in live cells The antibody has been designed to recognize TRPV3 from human rat and mouse samples
    Catalog Number:
    ACC-033
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs trpv3
    Anti TRPV3 extracellular Antibody
    Anti TRPV3 extracellular Antibody ACC 033 is a highly specific antibody directed against an extracellular epitope of human TRPV3 The antibody can be used in western blot immunohistochemical and immunocytochemical applications As the antibody recognizes an extracellular epitope it can be used as a tool to detect TRPV3 in live cells The antibody has been designed to recognize TRPV3 from human rat and mouse samples
    https://www.bioz.com/result/trpv3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpv3 - by Bioz Stars, 2021-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Recurrent activations of transient receptor potential vanilloid‐1 and vanilloid‐4 promote cellular proliferation and migration in esophageal squamous cell carcinoma cells
    Article Snippet: .. The membranes were incubated at 4 °C overnight with primary rabbit anti‐human TRPV1 (1 : 300, Alomone, Jerusalem, Israel, Cat#: ACC‐030), TRPV2 (1 : 500; Santa Cruz, CA, USA, Cat#: SC‐22520), TRPV3 (1 : 300, Alomone, Cat#: ACC‐033), and TRPV4 (1 : 500; Santa Cruz, CA, USA, Cat#: SC‐98592) antibodies, rabbit anti‐β‐actin antibody (1 : 2000; CST, Danvers, MA, USA, Cat#: 5125S), then washed by a solution containing (in mm ) 130 NaCl, 2.5 KCl, 10 Na2HPO4, 1.5 KH2PO4, 0.1% Tween‐20, and incubated with the horseradish peroxidase‐linked secondary antibodies (goat anti‐rabbit IgG, Beyotime, Nanjing, China) in 5% BSA (pH 7.4) for 2 h at room temperature. ..

    Article Title: Activation of transient receptor potential vanilloid 3 channel ( TRPV3) aggravated pathological cardiac hypertrophy via calcineurin/ NFATc3 pathway in rats, et al. Activation of transient receptor potential vanilloid 3 channel (TRPV3) aggravated pathological cardiac hypertrophy via calcineurin/NFATc3 pathway in rats
    Article Snippet: .. Subsequently, the sections were incubated with an anti‐TRPV3 antibody (1:200; Alomone labs, Jerusalem, Israel) overnight at 4°C. ..

    Staining:

    Article Title: Farnesyl Pyrophosphate Is a Novel Pain-producing Molecule via Specific Activation of TRPV3 *
    Article Snippet: .. DRG neuron and keratinocyte co-cultures were stained with rabbit polyclonal anti-TRPV3 (Alomon Labs, Jerusalem, Israel) for TRPV3-expressing cells, and Hoechst 33258 (Molecular Probes, Eugene, OR) for nuclei. ..

    Immunofluorescence:

    Article Title: PAR2 mediates itch via TRPV3 signaling in keratinocytes
    Article Snippet: .. Primary antibodies used for immunofluorescence of cultured primary keratinocytes were: anti-TRPV3 antibody (Alomone Labs, 1:300) and anti-PAR2 antibody (Santa Cruz, 1:100). ..

    Cell Culture:

    Article Title: PAR2 mediates itch via TRPV3 signaling in keratinocytes
    Article Snippet: .. Primary antibodies used for immunofluorescence of cultured primary keratinocytes were: anti-TRPV3 antibody (Alomone Labs, 1:300) and anti-PAR2 antibody (Santa Cruz, 1:100). ..

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  • 95
    Alomone Labs cav1 2
    Ca 2+ regulatory proteins in control and Sirt1 −/− ventricular myocytes. A, Representative immunoblot and mean data for <t>Cav1.2,</t> SERCA2a, NCX and CaMKII in control and Sirt1 −/− mice ventricular myocytes. B, Normalized densitometry for the protein levels (α‐Tubulin was used as an internal control; Control N = 6 and Sirt1 −/− N = 6; * P
    Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav1 2/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cav1 2 - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    93
    Alomone Labs anti trpv3 antibody
    <t>TRPV3</t> is involved in PAR2/Ca 2+ signaling in keratinocytes. ( a ) The percentages of responding keratinocytes from WT and Trpv3 −/− mice stimulated with histamine (100 μM) and SLIGRL (50 μM). ***p
    Anti Trpv3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpv3 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpv3 antibody - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Ca 2+ regulatory proteins in control and Sirt1 −/− ventricular myocytes. A, Representative immunoblot and mean data for Cav1.2, SERCA2a, NCX and CaMKII in control and Sirt1 −/− mice ventricular myocytes. B, Normalized densitometry for the protein levels (α‐Tubulin was used as an internal control; Control N = 6 and Sirt1 −/− N = 6; * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: The effect of Sirt1 deficiency on Ca2+ and Na+ regulation in mouse ventricular myocytes, et al. The effect of Sirt1 deficiency on Ca2+ and Na+ regulation in mouse ventricular myocytes

    doi: 10.1111/jcmm.15327

    Figure Lengend Snippet: Ca 2+ regulatory proteins in control and Sirt1 −/− ventricular myocytes. A, Representative immunoblot and mean data for Cav1.2, SERCA2a, NCX and CaMKII in control and Sirt1 −/− mice ventricular myocytes. B, Normalized densitometry for the protein levels (α‐Tubulin was used as an internal control; Control N = 6 and Sirt1 −/− N = 6; * P

    Article Snippet: The cardiac protein extracts were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and then transferred to polyvinylidene difluoride membranes (Millipore) that were incubated with the listed antibodies: Cav1.2 (1:1000, AACC‐033, rabbit polyclonal antibody; Alomone Labs, Jerusalem, Israel), CaMKII (1:1000, sc‐5306, mouse monoclonal antibody; Santa Cruz Biotechnology, Dallas, USA), NCX (1:1000, mouse monoclonal antibody, ab2869; Abcam), SERCA2a (1:5000, sc‐376235, mouse monoclonal antibody; Santa Cruz Biotechnology, Dallas, USA) and α‐tubulin (1:10 000, sc‐5286, mouse monoclonal antibody; Santa Cruz Biotechnology, Dallas, USA).

    Techniques: Mouse Assay

    TRPV3 is involved in PAR2/Ca 2+ signaling in keratinocytes. ( a ) The percentages of responding keratinocytes from WT and Trpv3 −/− mice stimulated with histamine (100 μM) and SLIGRL (50 μM). ***p

    Journal: The Journal of investigative dermatology

    Article Title: PAR2 mediates itch via TRPV3 signaling in keratinocytes

    doi: 10.1016/j.jid.2020.01.012

    Figure Lengend Snippet: TRPV3 is involved in PAR2/Ca 2+ signaling in keratinocytes. ( a ) The percentages of responding keratinocytes from WT and Trpv3 −/− mice stimulated with histamine (100 μM) and SLIGRL (50 μM). ***p

    Article Snippet: Primary antibodies used for immunofluorescence of cultured primary keratinocytes were: anti-TRPV3 antibody (Alomone Labs, 1:300) and anti-PAR2 antibody (Santa Cruz, 1:100).

    Techniques: Mouse Assay

    Topical blockade of PAR2 and TRPV3 attenuated the manifestations of AD mouse model. ( a-d ) Ear appearance of WT mice topically applied with vehicle as a control ( a ), MC-903 ( b ), MC-903+74a ( c ), and MC-903+FSLLRY ( d ) for 7 days. ( e-h ) Hematoxylin-eosin staining showing changes in hyperkeratosis and inflammatory infiltration in the ears of corresponding mice in panels a-d , respectively. Scale = 50 μm. ( i. j ) The scratching numbers ( i ) and ear thickness increment ( j ) of mice treated with MC-903, MC-903+74a and MC-903+FSLLRY for 7 days. One-way ANOVA followed by Tukey’s post hoc . n = 6–8 mice per group. ( k ) Diagram illustrating PAR2-TRPV3 signaling pathway in itch.

    Journal: The Journal of investigative dermatology

    Article Title: PAR2 mediates itch via TRPV3 signaling in keratinocytes

    doi: 10.1016/j.jid.2020.01.012

    Figure Lengend Snippet: Topical blockade of PAR2 and TRPV3 attenuated the manifestations of AD mouse model. ( a-d ) Ear appearance of WT mice topically applied with vehicle as a control ( a ), MC-903 ( b ), MC-903+74a ( c ), and MC-903+FSLLRY ( d ) for 7 days. ( e-h ) Hematoxylin-eosin staining showing changes in hyperkeratosis and inflammatory infiltration in the ears of corresponding mice in panels a-d , respectively. Scale = 50 μm. ( i. j ) The scratching numbers ( i ) and ear thickness increment ( j ) of mice treated with MC-903, MC-903+74a and MC-903+FSLLRY for 7 days. One-way ANOVA followed by Tukey’s post hoc . n = 6–8 mice per group. ( k ) Diagram illustrating PAR2-TRPV3 signaling pathway in itch.

    Article Snippet: Primary antibodies used for immunofluorescence of cultured primary keratinocytes were: anti-TRPV3 antibody (Alomone Labs, 1:300) and anti-PAR2 antibody (Santa Cruz, 1:100).

    Techniques: Mouse Assay, Staining

    TRPV3 is involved in PAR2-induced acute itch. ( a ) Acute itch behaviors of Trpv3 −/− mice and their WT littermates induced by intradermal injections of SLIGRL (50 μg), trypsin (100 μg), 2fly (10 μg), histamine (200 μg), and BAM 8-22 (150 μg). Unpaired t tests. n = 6–8. ( b ) WT mice were pre-injected with vehicle (Veh) (8% DMSO), 74a (150 μg, i.d.) or FSLLRY (100 μg, i.d.) 15 min before testing the scratching behaviors elicited by SLIGRL, trypsin, or 2fly. One-way ANOVA followed by Tukey’s post hoc . n = 6-8 mice per group. ( c ) TRPV3 expression in the skin and DRG of Trpv3 +/+ and Trpv3 −/− mice as determined by western blot. ( d ) Double immunofluorescence staining of PAR2 (green) and TRPV3 (red) in mouse keratinocytes. Scale bar, 20 μm. ( e ) Venn diagram showing the overlapping of PAR2 and TRPV3 in mouse keratinocytes. *p

    Journal: The Journal of investigative dermatology

    Article Title: PAR2 mediates itch via TRPV3 signaling in keratinocytes

    doi: 10.1016/j.jid.2020.01.012

    Figure Lengend Snippet: TRPV3 is involved in PAR2-induced acute itch. ( a ) Acute itch behaviors of Trpv3 −/− mice and their WT littermates induced by intradermal injections of SLIGRL (50 μg), trypsin (100 μg), 2fly (10 μg), histamine (200 μg), and BAM 8-22 (150 μg). Unpaired t tests. n = 6–8. ( b ) WT mice were pre-injected with vehicle (Veh) (8% DMSO), 74a (150 μg, i.d.) or FSLLRY (100 μg, i.d.) 15 min before testing the scratching behaviors elicited by SLIGRL, trypsin, or 2fly. One-way ANOVA followed by Tukey’s post hoc . n = 6-8 mice per group. ( c ) TRPV3 expression in the skin and DRG of Trpv3 +/+ and Trpv3 −/− mice as determined by western blot. ( d ) Double immunofluorescence staining of PAR2 (green) and TRPV3 (red) in mouse keratinocytes. Scale bar, 20 μm. ( e ) Venn diagram showing the overlapping of PAR2 and TRPV3 in mouse keratinocytes. *p

    Article Snippet: Primary antibodies used for immunofluorescence of cultured primary keratinocytes were: anti-TRPV3 antibody (Alomone Labs, 1:300) and anti-PAR2 antibody (Santa Cruz, 1:100).

    Techniques: Mouse Assay, Injection, Expressing, Western Blot, Double Immunofluorescence Staining

    The G-protein/PLC/Ca 2+ pathway mediated PAR2 activation in keratinocytes, and PAR2 and TRPV3 were necessary for adequate TSLP release from keratinocytes. ( a-c ) Representative traces showing that Ca 2+ transients elicited by SLIGRL (50 μM) in WT mouse keratinocytes were completely inhibited by co-incubation with 100 μM gallein, a G βγ -protein inhibitor ( a ) and 10 μM U73122, a PLC inhibitor ( b ), but were partially inhibited by co-incubation with 10 μM DBHQ, an ATPase inhibitor ( c ). ( d ) Quantified data showing the percentages of SLIGRL-responsive keratinocytes before and after the incubation of gallein, U73122 or DBHQ. *p

    Journal: The Journal of investigative dermatology

    Article Title: PAR2 mediates itch via TRPV3 signaling in keratinocytes

    doi: 10.1016/j.jid.2020.01.012

    Figure Lengend Snippet: The G-protein/PLC/Ca 2+ pathway mediated PAR2 activation in keratinocytes, and PAR2 and TRPV3 were necessary for adequate TSLP release from keratinocytes. ( a-c ) Representative traces showing that Ca 2+ transients elicited by SLIGRL (50 μM) in WT mouse keratinocytes were completely inhibited by co-incubation with 100 μM gallein, a G βγ -protein inhibitor ( a ) and 10 μM U73122, a PLC inhibitor ( b ), but were partially inhibited by co-incubation with 10 μM DBHQ, an ATPase inhibitor ( c ). ( d ) Quantified data showing the percentages of SLIGRL-responsive keratinocytes before and after the incubation of gallein, U73122 or DBHQ. *p

    Article Snippet: Primary antibodies used for immunofluorescence of cultured primary keratinocytes were: anti-TRPV3 antibody (Alomone Labs, 1:300) and anti-PAR2 antibody (Santa Cruz, 1:100).

    Techniques: Planar Chromatography, Activation Assay, Incubation

    PAR2 and TRPV3 were involved in the pathogenesis of chronic itch. ( a ) WT, Trpv3 −/− and Par2 −/− mice were treated daily with a topical application of MC-903. Scratching numbers were recorded on day 1, 3, 5, and 7. Two-way ANOVA followed by Bonferroni post hoc . n = 6–8 mice per group. ( b ) Real time RT-PCR showing the relative levels of TRPV3 and PAR2 mRNA in pruritic skin lesions of AD patients and normal control. Unpaired t test. n = 12. ( c, d ) Relative levels of PAR2 mRNA ( c ) and TRPV3 mRNA ( e ) in the ears of MC-903-treated mice on day 5. Two-way ANOVA with Bonferroni post hoc . n = 4–5. Data are presented as mean ± s.e.m. *p

    Journal: The Journal of investigative dermatology

    Article Title: PAR2 mediates itch via TRPV3 signaling in keratinocytes

    doi: 10.1016/j.jid.2020.01.012

    Figure Lengend Snippet: PAR2 and TRPV3 were involved in the pathogenesis of chronic itch. ( a ) WT, Trpv3 −/− and Par2 −/− mice were treated daily with a topical application of MC-903. Scratching numbers were recorded on day 1, 3, 5, and 7. Two-way ANOVA followed by Bonferroni post hoc . n = 6–8 mice per group. ( b ) Real time RT-PCR showing the relative levels of TRPV3 and PAR2 mRNA in pruritic skin lesions of AD patients and normal control. Unpaired t test. n = 12. ( c, d ) Relative levels of PAR2 mRNA ( c ) and TRPV3 mRNA ( e ) in the ears of MC-903-treated mice on day 5. Two-way ANOVA with Bonferroni post hoc . n = 4–5. Data are presented as mean ± s.e.m. *p

    Article Snippet: Primary antibodies used for immunofluorescence of cultured primary keratinocytes were: anti-TRPV3 antibody (Alomone Labs, 1:300) and anti-PAR2 antibody (Santa Cruz, 1:100).

    Techniques: Mouse Assay, Quantitative RT-PCR