guinea pig anti trpv1 vr1 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs guinea pig anti trpv1 vr1 antibody
    Photomicrographs of cryosections of canine skin showing transient receptor potential <t>vanilloid</t> 1 <t>(TRPV1)</t> immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic <t>TRPV1-IR.</t> The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.
    Guinea Pig Anti Trpv1 Vr1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti trpv1 vr1 antibody/product/Alomone Labs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti trpv1 vr1 antibody - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis"

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2022.915896

    Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.
    Figure Legend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.

    Techniques Used: Labeling

    Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P
    Figure Legend Snippet: Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P

    Techniques Used: Expressing

    Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.
    Figure Legend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.

    Techniques Used: Labeling

    2) Product Images from "Behavioral and nociceptor states of inflammatory pain across timescales in 2D and 3D"

    Article Title: Behavioral and nociceptor states of inflammatory pain across timescales in 2D and 3D

    Journal: bioRxiv

    doi: 10.1101/2021.06.16.448689

    Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel TRPV1 (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p
    Figure Legend Snippet: Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel TRPV1 (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p

    Techniques Used: Cell Culture, Injection

    3) Product Images from "Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia"

    Article Title: Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia

    Journal: JCI Insight

    doi: 10.1172/jci.insight.137386

    PD-L1 inhibits TRPV1 via SHP-1. ( A ) Schematic illustration of SHP-1–CKO mice by Cre-Lox system crossing SHP-1 fl/fl mice with Na V 1.8-Cre mice. ( B ) Double immunofluorescence reveals colocalization of Nav1.8 and SHP-1 in the L 4 DRG of control mice but no double staining signal of SHP-1 and Na V 1.8 in the L 4 DRG of SHP-1 CKO (bottom). Scale bar: 50 μm. ( C ) Western blot analysis showing a significant reduction of SHP-1 in the L 3 –L 5 DRGs of CKO mice. * P
    Figure Legend Snippet: PD-L1 inhibits TRPV1 via SHP-1. ( A ) Schematic illustration of SHP-1–CKO mice by Cre-Lox system crossing SHP-1 fl/fl mice with Na V 1.8-Cre mice. ( B ) Double immunofluorescence reveals colocalization of Nav1.8 and SHP-1 in the L 4 DRG of control mice but no double staining signal of SHP-1 and Na V 1.8 in the L 4 DRG of SHP-1 CKO (bottom). Scale bar: 50 μm. ( C ) Western blot analysis showing a significant reduction of SHP-1 in the L 3 –L 5 DRGs of CKO mice. * P

    Techniques Used: Mouse Assay, Immunofluorescence, Double Staining, Western Blot

    Dynamic changes in TRPV1 in DRG neurons after tumor inoculation and effects of PD-L1 on TRPV1 function. ( A ) Western blot analysis reveals a significant increase in the level of TRPV1 in L 3 –L 5 DRGs ipsilateral to the tumor-bearing bone after tumor inoculation. * P
    Figure Legend Snippet: Dynamic changes in TRPV1 in DRG neurons after tumor inoculation and effects of PD-L1 on TRPV1 function. ( A ) Western blot analysis reveals a significant increase in the level of TRPV1 in L 3 –L 5 DRGs ipsilateral to the tumor-bearing bone after tumor inoculation. * P

    Techniques Used: Western Blot

    Inhibition of SHP-1 sensitizes TRPV1 and facilitates pain-like behaviors. ( A ) Western blot analysis showing an increase in the level of phosphorylated SHP-1 (pSHP-1) in L 3 –L 5 DRGs ipsilateral to the tumor-bearing bone after tumor inoculation. * P
    Figure Legend Snippet: Inhibition of SHP-1 sensitizes TRPV1 and facilitates pain-like behaviors. ( A ) Western blot analysis showing an increase in the level of phosphorylated SHP-1 (pSHP-1) in L 3 –L 5 DRGs ipsilateral to the tumor-bearing bone after tumor inoculation. * P

    Techniques Used: Inhibition, Western Blot

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    Alomone Labs guinea pig anti trpv1 vr1 antibody
    Photomicrographs of cryosections of canine skin showing transient receptor potential <t>vanilloid</t> 1 <t>(TRPV1)</t> immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic <t>TRPV1-IR.</t> The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.
    Guinea Pig Anti Trpv1 Vr1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti trpv1 vr1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti trpv1 vr1 antibody - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

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    Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.

    Article Snippet: To identify the vanilloid receptor TRPV1, an antibody recently tested on the canine nervous system ( ) was used.

    Techniques: Labeling

    Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P

    Article Snippet: To identify the vanilloid receptor TRPV1, an antibody recently tested on the canine nervous system ( ) was used.

    Techniques: Expressing

    Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.

    Article Snippet: To identify the vanilloid receptor TRPV1, an antibody recently tested on the canine nervous system ( ) was used.

    Techniques: Labeling

    Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel TRPV1 (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p

    Journal: bioRxiv

    Article Title: Behavioral and nociceptor states of inflammatory pain across timescales in 2D and 3D

    doi: 10.1101/2021.06.16.448689

    Figure Lengend Snippet: Hind paw innervating sensory neurons cultured from the inflamed side are more excitable than those from the non-injected side during early inflammation. (A) Schematic representation of retrograde labelling of hind paw innervating sensory neurons with Fast Blue, Inset: Fast Blue positive neuron (blue) following acute dissociation, scale = 50 µm. (B) Relative frequency distributions of dissociated sensory neuron soma diameter from hind paw innervating and total lumbar populations, Insert: Proportion Fast Blue positive cells from acutely dissociated cultures of lumbar DRG. (C) Step-wise current injections were used to determine the rheobase of hind paw innervating sensory neurons from injected (ipsi) and non-injected (contra) sides 4- or 24-hours post-induction of inflammation with carrageenan. (D) Frequency of action potential (AP) discharge following stimulation of sensory neurons with a suprathreshold (2X rheobase). Representative traces from step-wise current injections to determine rheobase of neurons from (E) contralateral and (F) ipsilateral sides 4-hours post injection of carrageenan. (G) A subset of hind paw innervating sensory neurons (blue) express the nociceptive ion channel TRPV1 (magenta), cells positive for both Fast Blue and TRPV1 are identified by yellow pointers, scale = 50 µm. (H) A higher proportion of hind paw innervating neurons expressed TRPV1 after 24-hours of carrageenan-induced inflammation. ** p

    Article Snippet: After washing with PBS containing 0.001% (v/v) Tween-20 (Thermo Fisher Scientific), slides were incubated with antibody dilutant (1 % (w/v) BSA, 5% (v/v) donkey serum and 0.02% (v/v) Triton-X-100 in PBS) at room temperature for 1 hour before overnight incubation at 4 °C with an anti-TRPV1 antibody (1:500; guinea-pig polyclonal; Alomone, AGP-118).

    Techniques: Cell Culture, Injection

    PD-L1 inhibits TRPV1 via SHP-1. ( A ) Schematic illustration of SHP-1–CKO mice by Cre-Lox system crossing SHP-1 fl/fl mice with Na V 1.8-Cre mice. ( B ) Double immunofluorescence reveals colocalization of Nav1.8 and SHP-1 in the L 4 DRG of control mice but no double staining signal of SHP-1 and Na V 1.8 in the L 4 DRG of SHP-1 CKO (bottom). Scale bar: 50 μm. ( C ) Western blot analysis showing a significant reduction of SHP-1 in the L 3 –L 5 DRGs of CKO mice. * P

    Journal: JCI Insight

    Article Title: Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia

    doi: 10.1172/jci.insight.137386

    Figure Lengend Snippet: PD-L1 inhibits TRPV1 via SHP-1. ( A ) Schematic illustration of SHP-1–CKO mice by Cre-Lox system crossing SHP-1 fl/fl mice with Na V 1.8-Cre mice. ( B ) Double immunofluorescence reveals colocalization of Nav1.8 and SHP-1 in the L 4 DRG of control mice but no double staining signal of SHP-1 and Na V 1.8 in the L 4 DRG of SHP-1 CKO (bottom). Scale bar: 50 μm. ( C ) Western blot analysis showing a significant reduction of SHP-1 in the L 3 –L 5 DRGs of CKO mice. * P

    Article Snippet: The sections were blocked with 10% donkey serum in PBS, pH 7.4, with 0.3% Triton X-100 for 2 hours at room temperature (RT) and incubated for 24–36 hours at 4°C with the following primary antibodies: goat anti–PD-1 (1:500, R & D Systems, Bio-Techne, catalog AF1021, Minneapolis, Minnesota, USA), rabbit anti–Substance P (1:2000, Peninsula Laboratories, catalog T-4107.0050, San Carlos, California, USA), rabbit anti–SHP-1 (1:500, GeneTex, catalog GTX102864, Irvine, California, USA), goat anti-Nav1.8 (1:1000, Abgent, catalog AF3512a, San Diego, California, USA), guinea pig anti-TRPV1 (1:1000, Alomone Labs, catalog AGP-118, Jerusalem, Israel).

    Techniques: Mouse Assay, Immunofluorescence, Double Staining, Western Blot

    Dynamic changes in TRPV1 in DRG neurons after tumor inoculation and effects of PD-L1 on TRPV1 function. ( A ) Western blot analysis reveals a significant increase in the level of TRPV1 in L 3 –L 5 DRGs ipsilateral to the tumor-bearing bone after tumor inoculation. * P

    Journal: JCI Insight

    Article Title: Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia

    doi: 10.1172/jci.insight.137386

    Figure Lengend Snippet: Dynamic changes in TRPV1 in DRG neurons after tumor inoculation and effects of PD-L1 on TRPV1 function. ( A ) Western blot analysis reveals a significant increase in the level of TRPV1 in L 3 –L 5 DRGs ipsilateral to the tumor-bearing bone after tumor inoculation. * P

    Article Snippet: The sections were blocked with 10% donkey serum in PBS, pH 7.4, with 0.3% Triton X-100 for 2 hours at room temperature (RT) and incubated for 24–36 hours at 4°C with the following primary antibodies: goat anti–PD-1 (1:500, R & D Systems, Bio-Techne, catalog AF1021, Minneapolis, Minnesota, USA), rabbit anti–Substance P (1:2000, Peninsula Laboratories, catalog T-4107.0050, San Carlos, California, USA), rabbit anti–SHP-1 (1:500, GeneTex, catalog GTX102864, Irvine, California, USA), goat anti-Nav1.8 (1:1000, Abgent, catalog AF3512a, San Diego, California, USA), guinea pig anti-TRPV1 (1:1000, Alomone Labs, catalog AGP-118, Jerusalem, Israel).

    Techniques: Western Blot

    Inhibition of SHP-1 sensitizes TRPV1 and facilitates pain-like behaviors. ( A ) Western blot analysis showing an increase in the level of phosphorylated SHP-1 (pSHP-1) in L 3 –L 5 DRGs ipsilateral to the tumor-bearing bone after tumor inoculation. * P

    Journal: JCI Insight

    Article Title: Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia

    doi: 10.1172/jci.insight.137386

    Figure Lengend Snippet: Inhibition of SHP-1 sensitizes TRPV1 and facilitates pain-like behaviors. ( A ) Western blot analysis showing an increase in the level of phosphorylated SHP-1 (pSHP-1) in L 3 –L 5 DRGs ipsilateral to the tumor-bearing bone after tumor inoculation. * P

    Article Snippet: The sections were blocked with 10% donkey serum in PBS, pH 7.4, with 0.3% Triton X-100 for 2 hours at room temperature (RT) and incubated for 24–36 hours at 4°C with the following primary antibodies: goat anti–PD-1 (1:500, R & D Systems, Bio-Techne, catalog AF1021, Minneapolis, Minnesota, USA), rabbit anti–Substance P (1:2000, Peninsula Laboratories, catalog T-4107.0050, San Carlos, California, USA), rabbit anti–SHP-1 (1:500, GeneTex, catalog GTX102864, Irvine, California, USA), goat anti-Nav1.8 (1:1000, Abgent, catalog AF3512a, San Diego, California, USA), guinea pig anti-TRPV1 (1:1000, Alomone Labs, catalog AGP-118, Jerusalem, Israel).

    Techniques: Inhibition, Western Blot