Journal: International Journal of Clinical and Experimental Pathology
Article Title: Effect of cold and hot compress on neutrophilic migration to the site of doxorubicin extravasation
Figure Lengend Snippet: Reverse transcription-polymerase chain reaction (RT-PCR) for CD88, interleukin-8 RA (IL-8RA), and transient receptor potential cation channel subfamily V member 1 (TRPV1). mRNAs of CD88, IL-8RA, and TRPV1 including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were expressed in skin tissue on the first day after doxorubicin administration. MM: molecular marker, Lane 1; positive control (brain), Lane 2; control (no compress), Lane 3; cold compress, Lane 4; hot compress, Lane 5; negative control.
Article Snippet: We then fixed the sections with cold acetone (4°C) for 10 min, followed by addition of 3% skim milk (Morinaga Milk Industry, Tokyo, Japan) dissolved in PBS after washing in 0.01 M phosphate-buffered saline, pH 7.4 (PBS), for 15 min. For double immunofluorescence, slide-mounted tissue sections were incubated with a primary antibody [anti-CD88 (C5aR) antibody (rabbit, polyclonal, Santa Cruz Biotechnology, Santa Cruz, CA), anti-rat IL-8RA antibody (recognizing mouse IL-8RA, goat polyclonal, Santa Cruz Biotechnology), anti-rat TRPV1 (recognizing mouse TRPV1, rabbit, polyclonal, Alomone Labs, Jerusalem, Israel) and mouse monoclonal anti-α-SMA antibody (Clone 1A4, mouse IgG2a, Dako, Glostrup, Denmark)] overnight at 4°C.
Techniques: Reverse Transcription Polymerase Chain Reaction, Marker, Positive Control, Negative Control