anti rat trpv1 channel atto 488  (Alomone Labs)


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    Alomone Labs anti rat trpv1 channel atto 488
    <t>TRPV1</t> and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.
    Anti Rat Trpv1 Channel Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rat trpv1 channel atto 488/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rat trpv1 channel atto 488 - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats"

    Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S367193

    TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.
    Figure Legend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.

    Techniques Used: Expressing, Staining, Fluorescence

    TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P <0.001.
    Figure Legend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P <0.001.

    Techniques Used: Expressing, Staining, Fluorescence

    Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P <0.05, *** P <0.001.
    Figure Legend Snippet: Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P <0.05, *** P <0.001.

    Techniques Used: Expressing, Staining, Fluorescence

    anti rat trpv1 channel atto 488  (Alomone Labs)


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    Structured Review

    Alomone Labs anti rat trpv1 channel atto 488
    <t>TRPV1</t> and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.
    Anti Rat Trpv1 Channel Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rat trpv1 channel atto 488/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rat trpv1 channel atto 488 - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats"

    Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S367193

    TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.
    Figure Legend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.

    Techniques Used: Expressing, Staining, Fluorescence

    TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P <0.001.
    Figure Legend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P <0.001.

    Techniques Used: Expressing, Staining, Fluorescence

    Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P <0.05, *** P <0.001.
    Figure Legend Snippet: Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P <0.05, *** P <0.001.

    Techniques Used: Expressing, Staining, Fluorescence

    affinity purified anti trpv1 antibodies  (Alomone Labs)


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    Alomone Labs affinity purified anti trpv1 antibodies
    <t>TRPV1</t> gene and protein expression using RNA-sequencing data and Western blotting. A TRPV1 gene expression in the MILE dataset showing an increased TRPV1 expression in patients with different subtypes of ALL compared to normal bone marrow. B Overexpression of TRPV1 gene levels in AML pediatric patients with primary tumor and relapsed disease in the TARGET-AML dataset. All statistical analyses in A and B were performed in comparison to normal healthy subjects reported within each dataset (Dunnett’s multiple comparisons tests). C TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines, and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (~ 95 kDa) was detected in five patients (P#13, P14, P15, P33, P35)
    Affinity Purified Anti Trpv1 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified anti trpv1 antibodies/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    affinity purified anti trpv1 antibodies - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "Optimized flow cytometric detection of transient receptor potential vanilloid-1 (TRPV1) in human hematological malignancies"

    Article Title: Optimized flow cytometric detection of transient receptor potential vanilloid-1 (TRPV1) in human hematological malignancies

    Journal: Medical Oncology (Northwood, London, England)

    doi: 10.1007/s12032-022-01678-z

    TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. A TRPV1 gene expression in the MILE dataset showing an increased TRPV1 expression in patients with different subtypes of ALL compared to normal bone marrow. B Overexpression of TRPV1 gene levels in AML pediatric patients with primary tumor and relapsed disease in the TARGET-AML dataset. All statistical analyses in A and B were performed in comparison to normal healthy subjects reported within each dataset (Dunnett’s multiple comparisons tests). C TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines, and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (~ 95 kDa) was detected in five patients (P#13, P14, P15, P33, P35)
    Figure Legend Snippet: TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. A TRPV1 gene expression in the MILE dataset showing an increased TRPV1 expression in patients with different subtypes of ALL compared to normal bone marrow. B Overexpression of TRPV1 gene levels in AML pediatric patients with primary tumor and relapsed disease in the TARGET-AML dataset. All statistical analyses in A and B were performed in comparison to normal healthy subjects reported within each dataset (Dunnett’s multiple comparisons tests). C TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines, and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (~ 95 kDa) was detected in five patients (P#13, P14, P15, P33, P35)

    Techniques Used: Expressing, RNA Sequencing Assay, Western Blot, Over Expression, Positive Control

    TRPV1 expression using flow cytometry. A Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: B SC-20813, C ACC-030, and D LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells, and healthy human lymphocytes using LS-C150735. E.1 Flow cytometry analysis of TRPV1 expression levels in all patients with hematological malignancies ( n = 49) and healthy controls ( n = 20). Patients were then stratified and TRPV1 expression levels were plotted based on patients’ blood cancer type ( E.2 ) and treatment status ( E.3 ). TRPV1 in MM, B-NHL, and other hematological malignancies group (treated and de novo) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation is also shown. Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control ( P = 0.0351, unpaired t test) with B-NHL patients having significantly higher TRPV1 compared to control ( P = 0.0294, Dunnett’s test)
    Figure Legend Snippet: TRPV1 expression using flow cytometry. A Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: B SC-20813, C ACC-030, and D LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells, and healthy human lymphocytes using LS-C150735. E.1 Flow cytometry analysis of TRPV1 expression levels in all patients with hematological malignancies ( n = 49) and healthy controls ( n = 20). Patients were then stratified and TRPV1 expression levels were plotted based on patients’ blood cancer type ( E.2 ) and treatment status ( E.3 ). TRPV1 in MM, B-NHL, and other hematological malignancies group (treated and de novo) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation is also shown. Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control ( P = 0.0351, unpaired t test) with B-NHL patients having significantly higher TRPV1 compared to control ( P = 0.0294, Dunnett’s test)

    Techniques Used: Expressing, Flow Cytometry, Binding Assay, Fluorescence, Standard Deviation

    affinity purified anti trpv1 antibodies  (Alomone Labs)


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    Alomone Labs affinity purified anti trpv1 antibodies
    <t>TRPV1</t> gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P<0.0001 compared to all others (Tukey’s). (B) TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (∼ 95 kDa) was detected in five patients (P#13, P14, P15, P33 P35)
    Affinity Purified Anti Trpv1 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified anti trpv1 antibodies/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    affinity purified anti trpv1 antibodies - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies"

    Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies

    Journal: medRxiv

    doi: 10.1101/2021.08.04.21261521

    TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P<0.0001 compared to all others (Tukey’s). (B) TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (∼ 95 kDa) was detected in five patients (P#13, P14, P15, P33 P35)
    Figure Legend Snippet: TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P<0.0001 compared to all others (Tukey’s). (B) TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (∼ 95 kDa) was detected in five patients (P#13, P14, P15, P33 P35)

    Techniques Used: Expressing, RNA Sequencing Assay, Western Blot, Positive Control

    TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)
    Figure Legend Snippet: TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)

    Techniques Used: Expressing, Flow Cytometry, Binding Assay, Fluorescence, Standard Deviation

    rabbit anti rat trpv1 extracellular atto 488  (Alomone Labs)


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    Alomone Labs rabbit anti rat trpv1 extracellular atto 488
    Rabbit Anti Rat Trpv1 Extracellular Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat trpv1 extracellular atto 488/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat trpv1 extracellular atto 488 - by Bioz Stars, 2023-09
    93/100 stars

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    rabbit anti rat trpc polyclonal antibody subtypes  (Alomone Labs)


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    Alomone Labs rabbit anti rat trpc polyclonal antibody subtypes
    Rabbit Anti Rat Trpc Polyclonal Antibody Subtypes, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat trpc polyclonal antibody subtypes/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat trpc polyclonal antibody subtypes - by Bioz Stars, 2023-09
    93/100 stars

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    triton x 100  (Alomone Labs)


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    Alomone Labs triton x 100
    Triton X 100, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triton x 100/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triton x 100 - by Bioz Stars, 2023-09
    93/100 stars

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    rabbit anti rat trpc1 primary antibodies  (Alomone Labs)


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    Alomone Labs rabbit anti rat trpc1 primary antibodies
    T-PCR based detection of <t>TRPC1</t> in rat hearts. PCR products were observed in ethidium bromide-stained agarose gel. TRPC1 DNA fragments (467 bp) were amplified from left atrium, right atrium, left ventricle and right ventricle of rats.
    Rabbit Anti Rat Trpc1 Primary Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat trpc1 primary antibodies/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat trpc1 primary antibodies - by Bioz Stars, 2023-09
    93/100 stars

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    1) Product Images from "TRPC1 expression and distribution in rat hearts"

    Article Title: TRPC1 expression and distribution in rat hearts

    Journal: European Journal of Histochemistry : EJH

    doi: 10.4081/ejh.2009.e26

    T-PCR based detection of TRPC1 in rat hearts. PCR products were observed in ethidium bromide-stained agarose gel. TRPC1 DNA fragments (467 bp) were amplified from left atrium, right atrium, left ventricle and right ventricle of rats.
    Figure Legend Snippet: T-PCR based detection of TRPC1 in rat hearts. PCR products were observed in ethidium bromide-stained agarose gel. TRPC1 DNA fragments (467 bp) were amplified from left atrium, right atrium, left ventricle and right ventricle of rats.

    Techniques Used: Staining, Agarose Gel Electrophoresis, Amplification

    Immunohistochemical detection of TRPC1 protein in rat hearts. Sections were incubated with primary antibody for TRPC1 (A, B, C, D), without primary antibody (E, F, G, H) or with primary antibody preabsorbed by TRPC1 peptide for negative control (I). Positive signals in brown color can be visualized in the myocytes of the left ventricle (A) and atrium (B), endothelial and smooth muscle layers of coronary arterioles (C), and skeletal muscle cells (D, as positive control). No positive signal could be observed in control experiments without primary antibody. A faint signal was occasionally observed in antigen preabsorption control (I). There are negative cells in the edge of ventricular tissues (J) and also the fibroblasts between ventricular myocytes which showed blue nuclei without positive signals. The right ventricle shows the same distribution of TRPC1 positive signal (K) as the left ventricle. TRPC1 showed intense staining on the cell membranes of ventricular myocytes (A, K, L) and skeletal muscle cells (D). The longitudinal section of left ventricle also shows striated distribution of TRPC1 (L). Scale bar =10 µm, except scale bar = 50 µm in panel J.
    Figure Legend Snippet: Immunohistochemical detection of TRPC1 protein in rat hearts. Sections were incubated with primary antibody for TRPC1 (A, B, C, D), without primary antibody (E, F, G, H) or with primary antibody preabsorbed by TRPC1 peptide for negative control (I). Positive signals in brown color can be visualized in the myocytes of the left ventricle (A) and atrium (B), endothelial and smooth muscle layers of coronary arterioles (C), and skeletal muscle cells (D, as positive control). No positive signal could be observed in control experiments without primary antibody. A faint signal was occasionally observed in antigen preabsorption control (I). There are negative cells in the edge of ventricular tissues (J) and also the fibroblasts between ventricular myocytes which showed blue nuclei without positive signals. The right ventricle shows the same distribution of TRPC1 positive signal (K) as the left ventricle. TRPC1 showed intense staining on the cell membranes of ventricular myocytes (A, K, L) and skeletal muscle cells (D). The longitudinal section of left ventricle also shows striated distribution of TRPC1 (L). Scale bar =10 µm, except scale bar = 50 µm in panel J.

    Techniques Used: Immunohistochemical staining, Incubation, Negative Control, Positive Control, Staining

    Distribution of TRPC1 in Purkinje cells. These sections were contiguous tissue cross-sections. Endocardial layers were shown. Positive signals, brown in color, could be visualized in Purkinje cells (A, black arrows showed Purkinje cells). The cells beneath the endocardium with loose cytoplasmic structure and without any structure around nuclei were Purkinje cells according to HE staining (B, black arrows showed Purkinje cells). No positive signal could be observed in control experiments (C). Scale bar = 10 µm.
    Figure Legend Snippet: Distribution of TRPC1 in Purkinje cells. These sections were contiguous tissue cross-sections. Endocardial layers were shown. Positive signals, brown in color, could be visualized in Purkinje cells (A, black arrows showed Purkinje cells). The cells beneath the endocardium with loose cytoplasmic structure and without any structure around nuclei were Purkinje cells according to HE staining (B, black arrows showed Purkinje cells). No positive signal could be observed in control experiments (C). Scale bar = 10 µm.

    Techniques Used: Staining

    Localization of TRPC1 in rat cardiomyocytes shown by confocal images. Cardiac myocytes were double stained by anti-TRPC1 antibody (A and D) and phalloidin (B and E). Panels C and F show a merged image of panel A/B and D/E respectively, where TRPC1 is colored in red and phalloidin in green. The transverse striation of actin filaments can be seen both in the ventricular myocytes (B) and the atrial myocytes (E). Note that TRPC1 in the ventricular myocyte (A) are parallel to and close to transverse striation of actin filaments, suggesting that they are located at T-tubules while TRPC1 in the atrial myocytes (D) do not show the striation-like distribution. Scale bar =25 µm.
    Figure Legend Snippet: Localization of TRPC1 in rat cardiomyocytes shown by confocal images. Cardiac myocytes were double stained by anti-TRPC1 antibody (A and D) and phalloidin (B and E). Panels C and F show a merged image of panel A/B and D/E respectively, where TRPC1 is colored in red and phalloidin in green. The transverse striation of actin filaments can be seen both in the ventricular myocytes (B) and the atrial myocytes (E). Note that TRPC1 in the ventricular myocyte (A) are parallel to and close to transverse striation of actin filaments, suggesting that they are located at T-tubules while TRPC1 in the atrial myocytes (D) do not show the striation-like distribution. Scale bar =25 µm.

    Techniques Used: Staining

    rabbit anti rat trpc1 primary antibodies  (Alomone Labs)


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    Alomone Labs rabbit anti rat trpc1 primary antibodies
    Rabbit Anti Rat Trpc1 Primary Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal anti rat endothelin 1 a type receptors antibody  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti rat endothelin 1 a type receptors antibody
    Rabbit Polyclonal Anti Rat Endothelin 1 A Type Receptors Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti rat etb receptor polyclonal antibody  (Alomone Labs)


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    Alomone Labs rabbit anti rat etb receptor polyclonal antibody
    Rabbit Anti Rat Etb Receptor Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti rat trpv1 channel atto 488
    <t>TRPV1</t> and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.
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    Alomone Labs affinity purified anti trpv1 antibodies
    <t>TRPV1</t> gene and protein expression using RNA-sequencing data and Western blotting. A TRPV1 gene expression in the MILE dataset showing an increased TRPV1 expression in patients with different subtypes of ALL compared to normal bone marrow. B Overexpression of TRPV1 gene levels in AML pediatric patients with primary tumor and relapsed disease in the TARGET-AML dataset. All statistical analyses in A and B were performed in comparison to normal healthy subjects reported within each dataset (Dunnett’s multiple comparisons tests). C TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines, and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (~ 95 kDa) was detected in five patients (P#13, P14, P15, P33, P35)
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    <t>TRPV1</t> gene and protein expression using RNA-sequencing data and Western blotting. A TRPV1 gene expression in the MILE dataset showing an increased TRPV1 expression in patients with different subtypes of ALL compared to normal bone marrow. B Overexpression of TRPV1 gene levels in AML pediatric patients with primary tumor and relapsed disease in the TARGET-AML dataset. All statistical analyses in A and B were performed in comparison to normal healthy subjects reported within each dataset (Dunnett’s multiple comparisons tests). C TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines, and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (~ 95 kDa) was detected in five patients (P#13, P14, P15, P33, P35)
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    Alomone Labs rabbit anti rat trpc polyclonal antibody subtypes
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    <t>TRPV1</t> gene and protein expression using RNA-sequencing data and Western blotting. A TRPV1 gene expression in the MILE dataset showing an increased TRPV1 expression in patients with different subtypes of ALL compared to normal bone marrow. B Overexpression of TRPV1 gene levels in AML pediatric patients with primary tumor and relapsed disease in the TARGET-AML dataset. All statistical analyses in A and B were performed in comparison to normal healthy subjects reported within each dataset (Dunnett’s multiple comparisons tests). C TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines, and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (~ 95 kDa) was detected in five patients (P#13, P14, P15, P33, P35)
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    Alomone Labs rabbit anti rat trpc1 primary antibodies
    T-PCR based detection of <t>TRPC1</t> in rat hearts. PCR products were observed in ethidium bromide-stained agarose gel. TRPC1 DNA fragments (467 bp) were amplified from left atrium, right atrium, left ventricle and right ventricle of rats.
    Rabbit Anti Rat Trpc1 Primary Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs rabbit polyclonal anti rat endothelin 1 a type receptors antibody
    T-PCR based detection of <t>TRPC1</t> in rat hearts. PCR products were observed in ethidium bromide-stained agarose gel. TRPC1 DNA fragments (467 bp) were amplified from left atrium, right atrium, left ventricle and right ventricle of rats.
    Rabbit Polyclonal Anti Rat Endothelin 1 A Type Receptors Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    T-PCR based detection of <t>TRPC1</t> in rat hearts. PCR products were observed in ethidium bromide-stained agarose gel. TRPC1 DNA fragments (467 bp) were amplified from left atrium, right atrium, left ventricle and right ventricle of rats.
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    TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.

    Journal: Journal of Inflammation Research

    Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats

    doi: 10.2147/JIR.S367193

    Figure Lengend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.

    Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies: anti-rat TRPV1 channel ATTO-488 (1:240, cat.ACC-029-AG, Alomone labs, Jerusalem), anti-rat TRPA1 channel (1:240, cat.ACC-037, Alomone labs, Jerusalem), anti-rat protein gene product 9.5 (PGP 9.5, 1:500, NB300-676, Novus Biological), rabbit anti-Chymase (1:250, NBP2-257441, Novus Biological) and mouse anti-Tryptase (1:250, NBP2-26444, Novus Biological).

    Techniques: Expressing, Staining, Fluorescence

    TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P <0.001.

    Journal: Journal of Inflammation Research

    Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats

    doi: 10.2147/JIR.S367193

    Figure Lengend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P <0.001.

    Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies: anti-rat TRPV1 channel ATTO-488 (1:240, cat.ACC-029-AG, Alomone labs, Jerusalem), anti-rat TRPA1 channel (1:240, cat.ACC-037, Alomone labs, Jerusalem), anti-rat protein gene product 9.5 (PGP 9.5, 1:500, NB300-676, Novus Biological), rabbit anti-Chymase (1:250, NBP2-257441, Novus Biological) and mouse anti-Tryptase (1:250, NBP2-26444, Novus Biological).

    Techniques: Expressing, Staining, Fluorescence

    Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P <0.05, *** P <0.001.

    Journal: Journal of Inflammation Research

    Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats

    doi: 10.2147/JIR.S367193

    Figure Lengend Snippet: Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P <0.05, *** P <0.001.

    Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies: anti-rat TRPV1 channel ATTO-488 (1:240, cat.ACC-029-AG, Alomone labs, Jerusalem), anti-rat TRPA1 channel (1:240, cat.ACC-037, Alomone labs, Jerusalem), anti-rat protein gene product 9.5 (PGP 9.5, 1:500, NB300-676, Novus Biological), rabbit anti-Chymase (1:250, NBP2-257441, Novus Biological) and mouse anti-Tryptase (1:250, NBP2-26444, Novus Biological).

    Techniques: Expressing, Staining, Fluorescence

    TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. A TRPV1 gene expression in the MILE dataset showing an increased TRPV1 expression in patients with different subtypes of ALL compared to normal bone marrow. B Overexpression of TRPV1 gene levels in AML pediatric patients with primary tumor and relapsed disease in the TARGET-AML dataset. All statistical analyses in A and B were performed in comparison to normal healthy subjects reported within each dataset (Dunnett’s multiple comparisons tests). C TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines, and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (~ 95 kDa) was detected in five patients (P#13, P14, P15, P33, P35)

    Journal: Medical Oncology (Northwood, London, England)

    Article Title: Optimized flow cytometric detection of transient receptor potential vanilloid-1 (TRPV1) in human hematological malignancies

    doi: 10.1007/s12032-022-01678-z

    Figure Lengend Snippet: TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. A TRPV1 gene expression in the MILE dataset showing an increased TRPV1 expression in patients with different subtypes of ALL compared to normal bone marrow. B Overexpression of TRPV1 gene levels in AML pediatric patients with primary tumor and relapsed disease in the TARGET-AML dataset. All statistical analyses in A and B were performed in comparison to normal healthy subjects reported within each dataset (Dunnett’s multiple comparisons tests). C TRPV1 expression detected using Western blotting in THP-1 and U266B1 cell lines, and PBMCs protein samples of patients with hematological malignancies. THP-1 protein was used as a positive control, and GAPDH was used as an internal control. TRPV1 monomer (~ 95 kDa) was detected in five patients (P#13, P14, P15, P33, P35)

    Article Snippet: This was in contrast to the affinity purified anti-TRPV1 antibodies from Alomone Labs and Santa Cruz Biotechnology which were unable to separate the TRPV1 signal from the isotype control, suggesting a lack of specificity for TRPV1.

    Techniques: Expressing, RNA Sequencing Assay, Western Blot, Over Expression, Positive Control

    TRPV1 expression using flow cytometry. A Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: B SC-20813, C ACC-030, and D LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells, and healthy human lymphocytes using LS-C150735. E.1 Flow cytometry analysis of TRPV1 expression levels in all patients with hematological malignancies ( n = 49) and healthy controls ( n = 20). Patients were then stratified and TRPV1 expression levels were plotted based on patients’ blood cancer type ( E.2 ) and treatment status ( E.3 ). TRPV1 in MM, B-NHL, and other hematological malignancies group (treated and de novo) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation is also shown. Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control ( P = 0.0351, unpaired t test) with B-NHL patients having significantly higher TRPV1 compared to control ( P = 0.0294, Dunnett’s test)

    Journal: Medical Oncology (Northwood, London, England)

    Article Title: Optimized flow cytometric detection of transient receptor potential vanilloid-1 (TRPV1) in human hematological malignancies

    doi: 10.1007/s12032-022-01678-z

    Figure Lengend Snippet: TRPV1 expression using flow cytometry. A Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: B SC-20813, C ACC-030, and D LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells, and healthy human lymphocytes using LS-C150735. E.1 Flow cytometry analysis of TRPV1 expression levels in all patients with hematological malignancies ( n = 49) and healthy controls ( n = 20). Patients were then stratified and TRPV1 expression levels were plotted based on patients’ blood cancer type ( E.2 ) and treatment status ( E.3 ). TRPV1 in MM, B-NHL, and other hematological malignancies group (treated and de novo) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation is also shown. Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control ( P = 0.0351, unpaired t test) with B-NHL patients having significantly higher TRPV1 compared to control ( P = 0.0294, Dunnett’s test)

    Article Snippet: This was in contrast to the affinity purified anti-TRPV1 antibodies from Alomone Labs and Santa Cruz Biotechnology which were unable to separate the TRPV1 signal from the isotype control, suggesting a lack of specificity for TRPV1.

    Techniques: Expressing, Flow Cytometry, Binding Assay, Fluorescence, Standard Deviation

    T-PCR based detection of TRPC1 in rat hearts. PCR products were observed in ethidium bromide-stained agarose gel. TRPC1 DNA fragments (467 bp) were amplified from left atrium, right atrium, left ventricle and right ventricle of rats.

    Journal: European Journal of Histochemistry : EJH

    Article Title: TRPC1 expression and distribution in rat hearts

    doi: 10.4081/ejh.2009.e26

    Figure Lengend Snippet: T-PCR based detection of TRPC1 in rat hearts. PCR products were observed in ethidium bromide-stained agarose gel. TRPC1 DNA fragments (467 bp) were amplified from left atrium, right atrium, left ventricle and right ventricle of rats.

    Article Snippet: Sections were incubated at 4°C overnight with rabbit anti-rat TRPC1 primary antibodies (1:100 dilution, batch number AN-04, Alomone Labs, Jerusalem, Israel).

    Techniques: Staining, Agarose Gel Electrophoresis, Amplification

    Immunohistochemical detection of TRPC1 protein in rat hearts. Sections were incubated with primary antibody for TRPC1 (A, B, C, D), without primary antibody (E, F, G, H) or with primary antibody preabsorbed by TRPC1 peptide for negative control (I). Positive signals in brown color can be visualized in the myocytes of the left ventricle (A) and atrium (B), endothelial and smooth muscle layers of coronary arterioles (C), and skeletal muscle cells (D, as positive control). No positive signal could be observed in control experiments without primary antibody. A faint signal was occasionally observed in antigen preabsorption control (I). There are negative cells in the edge of ventricular tissues (J) and also the fibroblasts between ventricular myocytes which showed blue nuclei without positive signals. The right ventricle shows the same distribution of TRPC1 positive signal (K) as the left ventricle. TRPC1 showed intense staining on the cell membranes of ventricular myocytes (A, K, L) and skeletal muscle cells (D). The longitudinal section of left ventricle also shows striated distribution of TRPC1 (L). Scale bar =10 µm, except scale bar = 50 µm in panel J.

    Journal: European Journal of Histochemistry : EJH

    Article Title: TRPC1 expression and distribution in rat hearts

    doi: 10.4081/ejh.2009.e26

    Figure Lengend Snippet: Immunohistochemical detection of TRPC1 protein in rat hearts. Sections were incubated with primary antibody for TRPC1 (A, B, C, D), without primary antibody (E, F, G, H) or with primary antibody preabsorbed by TRPC1 peptide for negative control (I). Positive signals in brown color can be visualized in the myocytes of the left ventricle (A) and atrium (B), endothelial and smooth muscle layers of coronary arterioles (C), and skeletal muscle cells (D, as positive control). No positive signal could be observed in control experiments without primary antibody. A faint signal was occasionally observed in antigen preabsorption control (I). There are negative cells in the edge of ventricular tissues (J) and also the fibroblasts between ventricular myocytes which showed blue nuclei without positive signals. The right ventricle shows the same distribution of TRPC1 positive signal (K) as the left ventricle. TRPC1 showed intense staining on the cell membranes of ventricular myocytes (A, K, L) and skeletal muscle cells (D). The longitudinal section of left ventricle also shows striated distribution of TRPC1 (L). Scale bar =10 µm, except scale bar = 50 µm in panel J.

    Article Snippet: Sections were incubated at 4°C overnight with rabbit anti-rat TRPC1 primary antibodies (1:100 dilution, batch number AN-04, Alomone Labs, Jerusalem, Israel).

    Techniques: Immunohistochemical staining, Incubation, Negative Control, Positive Control, Staining

    Distribution of TRPC1 in Purkinje cells. These sections were contiguous tissue cross-sections. Endocardial layers were shown. Positive signals, brown in color, could be visualized in Purkinje cells (A, black arrows showed Purkinje cells). The cells beneath the endocardium with loose cytoplasmic structure and without any structure around nuclei were Purkinje cells according to HE staining (B, black arrows showed Purkinje cells). No positive signal could be observed in control experiments (C). Scale bar = 10 µm.

    Journal: European Journal of Histochemistry : EJH

    Article Title: TRPC1 expression and distribution in rat hearts

    doi: 10.4081/ejh.2009.e26

    Figure Lengend Snippet: Distribution of TRPC1 in Purkinje cells. These sections were contiguous tissue cross-sections. Endocardial layers were shown. Positive signals, brown in color, could be visualized in Purkinje cells (A, black arrows showed Purkinje cells). The cells beneath the endocardium with loose cytoplasmic structure and without any structure around nuclei were Purkinje cells according to HE staining (B, black arrows showed Purkinje cells). No positive signal could be observed in control experiments (C). Scale bar = 10 µm.

    Article Snippet: Sections were incubated at 4°C overnight with rabbit anti-rat TRPC1 primary antibodies (1:100 dilution, batch number AN-04, Alomone Labs, Jerusalem, Israel).

    Techniques: Staining

    Localization of TRPC1 in rat cardiomyocytes shown by confocal images. Cardiac myocytes were double stained by anti-TRPC1 antibody (A and D) and phalloidin (B and E). Panels C and F show a merged image of panel A/B and D/E respectively, where TRPC1 is colored in red and phalloidin in green. The transverse striation of actin filaments can be seen both in the ventricular myocytes (B) and the atrial myocytes (E). Note that TRPC1 in the ventricular myocyte (A) are parallel to and close to transverse striation of actin filaments, suggesting that they are located at T-tubules while TRPC1 in the atrial myocytes (D) do not show the striation-like distribution. Scale bar =25 µm.

    Journal: European Journal of Histochemistry : EJH

    Article Title: TRPC1 expression and distribution in rat hearts

    doi: 10.4081/ejh.2009.e26

    Figure Lengend Snippet: Localization of TRPC1 in rat cardiomyocytes shown by confocal images. Cardiac myocytes were double stained by anti-TRPC1 antibody (A and D) and phalloidin (B and E). Panels C and F show a merged image of panel A/B and D/E respectively, where TRPC1 is colored in red and phalloidin in green. The transverse striation of actin filaments can be seen both in the ventricular myocytes (B) and the atrial myocytes (E). Note that TRPC1 in the ventricular myocyte (A) are parallel to and close to transverse striation of actin filaments, suggesting that they are located at T-tubules while TRPC1 in the atrial myocytes (D) do not show the striation-like distribution. Scale bar =25 µm.

    Article Snippet: Sections were incubated at 4°C overnight with rabbit anti-rat TRPC1 primary antibodies (1:100 dilution, batch number AN-04, Alomone Labs, Jerusalem, Israel).

    Techniques: Staining