trpv1 antibody  (Alomone Labs)


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    Name:
    Anti Rat TRPV1 VR1 extracellular ATTO Fluor 488 Antibody
    Description:
    Anti Rat TRPV1 VR1 extracellular Antibody ACC 029 is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel The antibody can be used in western blot immunohistochemistry and live cell imaging applications It has been designed to recognize TRPV1 from rat samples only nAnti Rat TRPV1 VR1 extracellular ATTO Fluor 488 Antibody ACC 029 AG is directly labeled with an ATTO 488 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 488 label is analogous to the well known dye fluorescein isothiocyanate FITC and can be used with filters typically used to detect FITC Anti Rat TRPV1 VR1 extracellular ATTO Fluor 488 Antibody has been tested in immunohistochemistry and live cell imaging applications and is especially suited for experiments requiring simultaneous labeling of different markers
    Catalog Number:
    ACC-029-AG
    Price:
    686.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Live Cell Imaging
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal ATTO 488 (Green) Conjugated Primary Antibody
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs trpv1 antibody
    Anti Rat TRPV1 VR1 extracellular ATTO Fluor 488 Antibody
    Anti Rat TRPV1 VR1 extracellular Antibody ACC 029 is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel The antibody can be used in western blot immunohistochemistry and live cell imaging applications It has been designed to recognize TRPV1 from rat samples only nAnti Rat TRPV1 VR1 extracellular ATTO Fluor 488 Antibody ACC 029 AG is directly labeled with an ATTO 488 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability The ATTO 488 label is analogous to the well known dye fluorescein isothiocyanate FITC and can be used with filters typically used to detect FITC Anti Rat TRPV1 VR1 extracellular ATTO Fluor 488 Antibody has been tested in immunohistochemistry and live cell imaging applications and is especially suited for experiments requiring simultaneous labeling of different markers
    https://www.bioz.com/result/trpv1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpv1 antibody - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies"

    Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies

    Journal: medRxiv

    doi: 10.1101/2021.08.04.21261521

    TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P
    Figure Legend Snippet: TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P

    Techniques Used: Expressing, RNA Sequencing Assay, Western Blot

    TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)
    Figure Legend Snippet: TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)

    Techniques Used: Expressing, Flow Cytometry, Binding Assay, Fluorescence, Standard Deviation

    2) Product Images from "Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury"

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.62.9.6

    Recovery of TRPV1 + nerves one week after a sterile epithelial abrasion ( A – L ). The distribution of βIII + TRPV1 + nerves was different across the central ( A – F ) and peripheral cornea ( G – L ). ( M , N ) One-week post-injury, the sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P
    Figure Legend Snippet: Recovery of TRPV1 + nerves one week after a sterile epithelial abrasion ( A – L ). The distribution of βIII + TRPV1 + nerves was different across the central ( A – F ) and peripheral cornea ( G – L ). ( M , N ) One-week post-injury, the sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P

    Techniques Used:

    TRPV1 expression in wild type (WT) and TRPV1 −/− mice. Localization of TRPV1 in corneal nerves and its intracellular localization in the stromal macrophages in wild type mouse corneas ( A, B ), and absent in the TRPV1 −/− mouse cornea ( C, D ). The punctate staining in the epithelium was present in the TRPV1 −/− mice, likely representing nonspecific background staining. Scale bars represent 50 µm for all images. ( E, F ) Punctate TRPV1 signals in the CD45 + stromal macrophages were present in the WT but absent in the TRPV1 −/− mouse corneas ( G ).
    Figure Legend Snippet: TRPV1 expression in wild type (WT) and TRPV1 −/− mice. Localization of TRPV1 in corneal nerves and its intracellular localization in the stromal macrophages in wild type mouse corneas ( A, B ), and absent in the TRPV1 −/− mouse cornea ( C, D ). The punctate staining in the epithelium was present in the TRPV1 −/− mice, likely representing nonspecific background staining. Scale bars represent 50 µm for all images. ( E, F ) Punctate TRPV1 signals in the CD45 + stromal macrophages were present in the WT but absent in the TRPV1 −/− mouse corneas ( G ).

    Techniques Used: Expressing, Mouse Assay, Staining

    TRPV1 localization in nonneuronal cell bodies and their partial overlap with lysosomes of the stromal macrophages. ( A–C ) βIII + stromal nerve trunks expressed TRPV1. Intracellular TRPV1 staining was present in the macrophages that were in various depths of the stroma (color-coded depth projection, anterior: blue/purple; posterior: yellow) ( B , arrowheads; C , asterisks). ( D ) Absence of TRPV1 staining in tissues treated with peptide control antigen alongside positive staining of stromal CD45 + cells (red). ( E1–4 ) High resolution image of TRPV1 staining within the CD45 + stromal macrophages. ( F1–4 ) TRPV1 partially overlapped with the macrophage intracellular compartment that expresses CD68. ( G ) Orthogonal view of a CD45 + TRPV1 + stromal macrophage displaying the nonneuronal TRPV1 localization in both XY and XZ planes of the z-stack. ( H ) Orthogonal view of a CD68 + TRPV1 + stromal macrophage shows the overlap between lysosomes and TRPV1 proteins. Scale bars represent 100 µm ( A – D ) and 10 µm (E1 – 4, F1 – 4, G, H).
    Figure Legend Snippet: TRPV1 localization in nonneuronal cell bodies and their partial overlap with lysosomes of the stromal macrophages. ( A–C ) βIII + stromal nerve trunks expressed TRPV1. Intracellular TRPV1 staining was present in the macrophages that were in various depths of the stroma (color-coded depth projection, anterior: blue/purple; posterior: yellow) ( B , arrowheads; C , asterisks). ( D ) Absence of TRPV1 staining in tissues treated with peptide control antigen alongside positive staining of stromal CD45 + cells (red). ( E1–4 ) High resolution image of TRPV1 staining within the CD45 + stromal macrophages. ( F1–4 ) TRPV1 partially overlapped with the macrophage intracellular compartment that expresses CD68. ( G ) Orthogonal view of a CD45 + TRPV1 + stromal macrophage displaying the nonneuronal TRPV1 localization in both XY and XZ planes of the z-stack. ( H ) Orthogonal view of a CD68 + TRPV1 + stromal macrophage shows the overlap between lysosomes and TRPV1 proteins. Scale bars represent 100 µm ( A – D ) and 10 µm (E1 – 4, F1 – 4, G, H).

    Techniques Used: Staining

    Epithelial TRPV1 + nerves and resident dendritic cells (DCs) under steady state conditions. ( A ) Representative images from the peripheral corneal epithelium showing resident intraepithelial CD45 + DCs in close proximity to TRPV1 + axons (asterisk), while some DCs did not appear to associate with TRPV1 + axons (arrowheads). ( B ) Orthogonal view of an intraepithelial CD45 + DC highlights the contacts between the TRPV1 + axons and DC in XYZ planes. ( C, D ) The absence of TRPV1 nerve staining in the peptide control validates the positive corneal nerve staining for TRPV1. ( E–H ) The resident DCs were immunopositive for CD11c and MHC class II. Scale bars represent 50 µm ( A , C , D ) and 10 µm ( E , F , G , H ).
    Figure Legend Snippet: Epithelial TRPV1 + nerves and resident dendritic cells (DCs) under steady state conditions. ( A ) Representative images from the peripheral corneal epithelium showing resident intraepithelial CD45 + DCs in close proximity to TRPV1 + axons (asterisk), while some DCs did not appear to associate with TRPV1 + axons (arrowheads). ( B ) Orthogonal view of an intraepithelial CD45 + DC highlights the contacts between the TRPV1 + axons and DC in XYZ planes. ( C, D ) The absence of TRPV1 nerve staining in the peptide control validates the positive corneal nerve staining for TRPV1. ( E–H ) The resident DCs were immunopositive for CD11c and MHC class II. Scale bars represent 50 µm ( A , C , D ) and 10 µm ( E , F , G , H ).

    Techniques Used: Staining

    Intraepithelial CD45 + dendriform cells in intact and injured corneas. ( A–D ) βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerve populations and their anatomical associations with DCs in the central and peripheral epithelium of intact ( A , B ) and injured corneas ( C , D ). ( E–L ) 3D image reconstructions show DC-nerve contacts between epithelial CD45 + DCs and βIII + TRPV1 + SBNP nerve axons (green: βIII; white: TRPV1). ( G–H ) DC-nerve complexes between DC and nerve axons (white area: βIII; yellow: TRPV1) were found within the DC dendrite. ( I–L ) One week after the epithelial abrasion, the degree of neuroimmune contacts between the DCs and nerve axons was similar to that observed in intact corneas (white area: βIII; blue: TRPV1) and DC dendrites. ( M, N ) The percentage of TRPV1 + nerve-associated DC was significantly higher across both central and peripheral corneas in the injured group compared to the intact control corneas ( P
    Figure Legend Snippet: Intraepithelial CD45 + dendriform cells in intact and injured corneas. ( A–D ) βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerve populations and their anatomical associations with DCs in the central and peripheral epithelium of intact ( A , B ) and injured corneas ( C , D ). ( E–L ) 3D image reconstructions show DC-nerve contacts between epithelial CD45 + DCs and βIII + TRPV1 + SBNP nerve axons (green: βIII; white: TRPV1). ( G–H ) DC-nerve complexes between DC and nerve axons (white area: βIII; yellow: TRPV1) were found within the DC dendrite. ( I–L ) One week after the epithelial abrasion, the degree of neuroimmune contacts between the DCs and nerve axons was similar to that observed in intact corneas (white area: βIII; blue: TRPV1) and DC dendrites. ( M, N ) The percentage of TRPV1 + nerve-associated DC was significantly higher across both central and peripheral corneas in the injured group compared to the intact control corneas ( P

    Techniques Used: Staining

    Topographical distribution of TRPV1 + nerves in the intact mouse cornea. ( A–L ) Distribution of βIII + ( A , D , G , and J ) and TRPV1 + ( B , E , H , and K ) superficial nerve terminals (SNT) and subbasal nerve plexus (SBNP) in the central ( A–F ) and peripheral corneal epithelium ( G – L ). False colored merged images demonstrate the relationship between βIII + TRPV1 + (cyan) and βIII + TRPV1 − (magenta) nerve fibers ( C , F , I , and L ). ( M – P ) The sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P
    Figure Legend Snippet: Topographical distribution of TRPV1 + nerves in the intact mouse cornea. ( A–L ) Distribution of βIII + ( A , D , G , and J ) and TRPV1 + ( B , E , H , and K ) superficial nerve terminals (SNT) and subbasal nerve plexus (SBNP) in the central ( A–F ) and peripheral corneal epithelium ( G – L ). False colored merged images demonstrate the relationship between βIII + TRPV1 + (cyan) and βIII + TRPV1 − (magenta) nerve fibers ( C , F , I , and L ). ( M – P ) The sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P

    Techniques Used:

    Stromal TRPV1 + nerves and CD45 + TRPV1 + macrophages in intact and injured corneas. ( A–D ) Superimposed images of βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerves and their anatomical interaction with macrophages in central and peripheral stroma of intact ( A , B ) and injured corneas ( C , D ). ( E–H ) The sum lengths of TRPV1 + stromal nerves were higher in both central and peripheral regions of the intact corneas ( P
    Figure Legend Snippet: Stromal TRPV1 + nerves and CD45 + TRPV1 + macrophages in intact and injured corneas. ( A–D ) Superimposed images of βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerves and their anatomical interaction with macrophages in central and peripheral stroma of intact ( A , B ) and injured corneas ( C , D ). ( E–H ) The sum lengths of TRPV1 + stromal nerves were higher in both central and peripheral regions of the intact corneas ( P

    Techniques Used: Staining

    Related Articles

    Negative Control:

    Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies
    Article Snippet: .. Exclusion of TRPV1 antibody was used as an additional negative control. ..

    Immunostaining:

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury
    Article Snippet: .. To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above. ..

    Incubation:

    Article Title: Alleviation of Trigeminal Nociception Using p75 Neurotrophin Receptor Targeted Lentiviral Interference Therapy
    Article Snippet: .. TrkA, p75NTR, VR1, vesicular stomatitis virus glycoprotein G (VSV-G), GFP, and phosphorylated Erk 1/2 were detected with overnight incubation of Nytran membranes at 4 °C with the following antibodies (1:500 in blocking reagent): rabbit anti-TrkA (mAb; Abcam), rabbit anti-p75 nerve growth factor (NGF) receptor (Abcam), rabbit anti-rat TRPV1 (extracellular)-ATTO-488 (Alomone Labs), rabbit anti-VSV-G (Abcam), goat anti-GFP (Abcam), and rabbit anti-Erk 1/2 (Cell Signaling) antibodies, respectively. ..

    Blocking Assay:

    Article Title: Alleviation of Trigeminal Nociception Using p75 Neurotrophin Receptor Targeted Lentiviral Interference Therapy
    Article Snippet: .. TrkA, p75NTR, VR1, vesicular stomatitis virus glycoprotein G (VSV-G), GFP, and phosphorylated Erk 1/2 were detected with overnight incubation of Nytran membranes at 4 °C with the following antibodies (1:500 in blocking reagent): rabbit anti-TrkA (mAb; Abcam), rabbit anti-p75 nerve growth factor (NGF) receptor (Abcam), rabbit anti-rat TRPV1 (extracellular)-ATTO-488 (Alomone Labs), rabbit anti-VSV-G (Abcam), goat anti-GFP (Abcam), and rabbit anti-Erk 1/2 (Cell Signaling) antibodies, respectively. ..

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  • 94
    Alomone Labs trpv1 antibody
    <t>TRPV1</t> gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P
    Trpv1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpv1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpv1 antibody - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P

    Journal: medRxiv

    Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies

    doi: 10.1101/2021.08.04.21261521

    Figure Lengend Snippet: TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P

    Article Snippet: Exclusion of TRPV1 antibody was used as an additional negative control.

    Techniques: Expressing, RNA Sequencing Assay, Western Blot

    TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)

    Journal: medRxiv

    Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies

    doi: 10.1101/2021.08.04.21261521

    Figure Lengend Snippet: TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)

    Article Snippet: Exclusion of TRPV1 antibody was used as an additional negative control.

    Techniques: Expressing, Flow Cytometry, Binding Assay, Fluorescence, Standard Deviation

    Recovery of TRPV1 + nerves one week after a sterile epithelial abrasion ( A – L ). The distribution of βIII + TRPV1 + nerves was different across the central ( A – F ) and peripheral cornea ( G – L ). ( M , N ) One-week post-injury, the sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

    doi: 10.1167/iovs.62.9.6

    Figure Lengend Snippet: Recovery of TRPV1 + nerves one week after a sterile epithelial abrasion ( A – L ). The distribution of βIII + TRPV1 + nerves was different across the central ( A – F ) and peripheral cornea ( G – L ). ( M , N ) One-week post-injury, the sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P

    Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

    Techniques:

    TRPV1 expression in wild type (WT) and TRPV1 −/− mice. Localization of TRPV1 in corneal nerves and its intracellular localization in the stromal macrophages in wild type mouse corneas ( A, B ), and absent in the TRPV1 −/− mouse cornea ( C, D ). The punctate staining in the epithelium was present in the TRPV1 −/− mice, likely representing nonspecific background staining. Scale bars represent 50 µm for all images. ( E, F ) Punctate TRPV1 signals in the CD45 + stromal macrophages were present in the WT but absent in the TRPV1 −/− mouse corneas ( G ).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

    doi: 10.1167/iovs.62.9.6

    Figure Lengend Snippet: TRPV1 expression in wild type (WT) and TRPV1 −/− mice. Localization of TRPV1 in corneal nerves and its intracellular localization in the stromal macrophages in wild type mouse corneas ( A, B ), and absent in the TRPV1 −/− mouse cornea ( C, D ). The punctate staining in the epithelium was present in the TRPV1 −/− mice, likely representing nonspecific background staining. Scale bars represent 50 µm for all images. ( E, F ) Punctate TRPV1 signals in the CD45 + stromal macrophages were present in the WT but absent in the TRPV1 −/− mouse corneas ( G ).

    Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

    Techniques: Expressing, Mouse Assay, Staining

    TRPV1 localization in nonneuronal cell bodies and their partial overlap with lysosomes of the stromal macrophages. ( A–C ) βIII + stromal nerve trunks expressed TRPV1. Intracellular TRPV1 staining was present in the macrophages that were in various depths of the stroma (color-coded depth projection, anterior: blue/purple; posterior: yellow) ( B , arrowheads; C , asterisks). ( D ) Absence of TRPV1 staining in tissues treated with peptide control antigen alongside positive staining of stromal CD45 + cells (red). ( E1–4 ) High resolution image of TRPV1 staining within the CD45 + stromal macrophages. ( F1–4 ) TRPV1 partially overlapped with the macrophage intracellular compartment that expresses CD68. ( G ) Orthogonal view of a CD45 + TRPV1 + stromal macrophage displaying the nonneuronal TRPV1 localization in both XY and XZ planes of the z-stack. ( H ) Orthogonal view of a CD68 + TRPV1 + stromal macrophage shows the overlap between lysosomes and TRPV1 proteins. Scale bars represent 100 µm ( A – D ) and 10 µm (E1 – 4, F1 – 4, G, H).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

    doi: 10.1167/iovs.62.9.6

    Figure Lengend Snippet: TRPV1 localization in nonneuronal cell bodies and their partial overlap with lysosomes of the stromal macrophages. ( A–C ) βIII + stromal nerve trunks expressed TRPV1. Intracellular TRPV1 staining was present in the macrophages that were in various depths of the stroma (color-coded depth projection, anterior: blue/purple; posterior: yellow) ( B , arrowheads; C , asterisks). ( D ) Absence of TRPV1 staining in tissues treated with peptide control antigen alongside positive staining of stromal CD45 + cells (red). ( E1–4 ) High resolution image of TRPV1 staining within the CD45 + stromal macrophages. ( F1–4 ) TRPV1 partially overlapped with the macrophage intracellular compartment that expresses CD68. ( G ) Orthogonal view of a CD45 + TRPV1 + stromal macrophage displaying the nonneuronal TRPV1 localization in both XY and XZ planes of the z-stack. ( H ) Orthogonal view of a CD68 + TRPV1 + stromal macrophage shows the overlap between lysosomes and TRPV1 proteins. Scale bars represent 100 µm ( A – D ) and 10 µm (E1 – 4, F1 – 4, G, H).

    Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

    Techniques: Staining

    Epithelial TRPV1 + nerves and resident dendritic cells (DCs) under steady state conditions. ( A ) Representative images from the peripheral corneal epithelium showing resident intraepithelial CD45 + DCs in close proximity to TRPV1 + axons (asterisk), while some DCs did not appear to associate with TRPV1 + axons (arrowheads). ( B ) Orthogonal view of an intraepithelial CD45 + DC highlights the contacts between the TRPV1 + axons and DC in XYZ planes. ( C, D ) The absence of TRPV1 nerve staining in the peptide control validates the positive corneal nerve staining for TRPV1. ( E–H ) The resident DCs were immunopositive for CD11c and MHC class II. Scale bars represent 50 µm ( A , C , D ) and 10 µm ( E , F , G , H ).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

    doi: 10.1167/iovs.62.9.6

    Figure Lengend Snippet: Epithelial TRPV1 + nerves and resident dendritic cells (DCs) under steady state conditions. ( A ) Representative images from the peripheral corneal epithelium showing resident intraepithelial CD45 + DCs in close proximity to TRPV1 + axons (asterisk), while some DCs did not appear to associate with TRPV1 + axons (arrowheads). ( B ) Orthogonal view of an intraepithelial CD45 + DC highlights the contacts between the TRPV1 + axons and DC in XYZ planes. ( C, D ) The absence of TRPV1 nerve staining in the peptide control validates the positive corneal nerve staining for TRPV1. ( E–H ) The resident DCs were immunopositive for CD11c and MHC class II. Scale bars represent 50 µm ( A , C , D ) and 10 µm ( E , F , G , H ).

    Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

    Techniques: Staining

    Intraepithelial CD45 + dendriform cells in intact and injured corneas. ( A–D ) βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerve populations and their anatomical associations with DCs in the central and peripheral epithelium of intact ( A , B ) and injured corneas ( C , D ). ( E–L ) 3D image reconstructions show DC-nerve contacts between epithelial CD45 + DCs and βIII + TRPV1 + SBNP nerve axons (green: βIII; white: TRPV1). ( G–H ) DC-nerve complexes between DC and nerve axons (white area: βIII; yellow: TRPV1) were found within the DC dendrite. ( I–L ) One week after the epithelial abrasion, the degree of neuroimmune contacts between the DCs and nerve axons was similar to that observed in intact corneas (white area: βIII; blue: TRPV1) and DC dendrites. ( M, N ) The percentage of TRPV1 + nerve-associated DC was significantly higher across both central and peripheral corneas in the injured group compared to the intact control corneas ( P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

    doi: 10.1167/iovs.62.9.6

    Figure Lengend Snippet: Intraepithelial CD45 + dendriform cells in intact and injured corneas. ( A–D ) βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerve populations and their anatomical associations with DCs in the central and peripheral epithelium of intact ( A , B ) and injured corneas ( C , D ). ( E–L ) 3D image reconstructions show DC-nerve contacts between epithelial CD45 + DCs and βIII + TRPV1 + SBNP nerve axons (green: βIII; white: TRPV1). ( G–H ) DC-nerve complexes between DC and nerve axons (white area: βIII; yellow: TRPV1) were found within the DC dendrite. ( I–L ) One week after the epithelial abrasion, the degree of neuroimmune contacts between the DCs and nerve axons was similar to that observed in intact corneas (white area: βIII; blue: TRPV1) and DC dendrites. ( M, N ) The percentage of TRPV1 + nerve-associated DC was significantly higher across both central and peripheral corneas in the injured group compared to the intact control corneas ( P

    Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

    Techniques: Staining

    Topographical distribution of TRPV1 + nerves in the intact mouse cornea. ( A–L ) Distribution of βIII + ( A , D , G , and J ) and TRPV1 + ( B , E , H , and K ) superficial nerve terminals (SNT) and subbasal nerve plexus (SBNP) in the central ( A–F ) and peripheral corneal epithelium ( G – L ). False colored merged images demonstrate the relationship between βIII + TRPV1 + (cyan) and βIII + TRPV1 − (magenta) nerve fibers ( C , F , I , and L ). ( M – P ) The sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

    doi: 10.1167/iovs.62.9.6

    Figure Lengend Snippet: Topographical distribution of TRPV1 + nerves in the intact mouse cornea. ( A–L ) Distribution of βIII + ( A , D , G , and J ) and TRPV1 + ( B , E , H , and K ) superficial nerve terminals (SNT) and subbasal nerve plexus (SBNP) in the central ( A–F ) and peripheral corneal epithelium ( G – L ). False colored merged images demonstrate the relationship between βIII + TRPV1 + (cyan) and βIII + TRPV1 − (magenta) nerve fibers ( C , F , I , and L ). ( M – P ) The sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P

    Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

    Techniques:

    Stromal TRPV1 + nerves and CD45 + TRPV1 + macrophages in intact and injured corneas. ( A–D ) Superimposed images of βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerves and their anatomical interaction with macrophages in central and peripheral stroma of intact ( A , B ) and injured corneas ( C , D ). ( E–H ) The sum lengths of TRPV1 + stromal nerves were higher in both central and peripheral regions of the intact corneas ( P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

    doi: 10.1167/iovs.62.9.6

    Figure Lengend Snippet: Stromal TRPV1 + nerves and CD45 + TRPV1 + macrophages in intact and injured corneas. ( A–D ) Superimposed images of βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerves and their anatomical interaction with macrophages in central and peripheral stroma of intact ( A , B ) and injured corneas ( C , D ). ( E–H ) The sum lengths of TRPV1 + stromal nerves were higher in both central and peripheral regions of the intact corneas ( P

    Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

    Techniques: Staining