trpv1 antibody  (Alomone Labs)

Journal: medRxiv

Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies

doi: 10.1101/2021.08.04.21261521

Figure Lengend Snippet: TRPV1 gene and protein expression using RNA-sequencing data and Western blotting. (A) TRPV1 gene expression in different databases. *P

Article Snippet: Exclusion of TRPV1 antibody was used as an additional negative control.

Techniques: Expressing, RNA Sequencing Assay, Western Blot

Journal: medRxiv

Article Title: Optimized Flow Cytometric Detection of Transient Receptor Potential Vanilloid-1 (TRPV1) in Human Hematological Malignancies

doi: 10.1101/2021.08.04.21261521

Figure Lengend Snippet: TRPV1 expression using Flow Cytometry. (A) Overview structure of TRPV1 and the 3 antibodies binding sites. Assessment of anti-TRPV1 antibodies: (B) SC-20813, (C) ACC-030, and (D) LS-C150735. Panel D demonstrates clear separation of isotype control from TRPV1 signals in THP-1, U266B1, U937 cells and healthy human lymphocytes using LS-C150735. (E) TRPV1 expression in all patients with hematological malignancies and healthy controls (n=20). TRPV1 in MM, B-NHL and other hematological malignancies group (treated and de novo ) were compared to control. Median fluorescence intensity (MFI) value for individual patients expressed using violin plots with the mean and standard deviation also shown. Patients samples were also expressed as a ratio (MFI patient: MFI control). Overall TRPV1 was significantly higher in all patients with hematological malignancies compared to healthy control (P=0.0351, unpaired t-test) with B-NHL patients having significantly higher TRPV1 compared to control (P= 0.0294, Dunnet’s test)

Article Snippet: Exclusion of TRPV1 antibody was used as an additional negative control.

Techniques: Expressing, Flow Cytometry, Binding Assay, Fluorescence, Standard Deviation

Journal: Investigative Ophthalmology & Visual Science

Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

doi: 10.1167/iovs.62.9.6

Figure Lengend Snippet: Recovery of TRPV1 + nerves one week after a sterile epithelial abrasion ( A – L ). The distribution of βIII + TRPV1 + nerves was different across the central ( A – F ) and peripheral cornea ( G – L ). ( M , N ) One-week post-injury, the sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P

Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

Techniques:

Journal: Investigative Ophthalmology & Visual Science

Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

doi: 10.1167/iovs.62.9.6

Figure Lengend Snippet: TRPV1 expression in wild type (WT) and TRPV1 −/− mice. Localization of TRPV1 in corneal nerves and its intracellular localization in the stromal macrophages in wild type mouse corneas ( A, B ), and absent in the TRPV1 −/− mouse cornea ( C, D ). The punctate staining in the epithelium was present in the TRPV1 −/− mice, likely representing nonspecific background staining. Scale bars represent 50 µm for all images. ( E, F ) Punctate TRPV1 signals in the CD45 + stromal macrophages were present in the WT but absent in the TRPV1 −/− mouse corneas ( G ).

Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

Techniques: Expressing, Mouse Assay, Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

doi: 10.1167/iovs.62.9.6

Figure Lengend Snippet: TRPV1 localization in nonneuronal cell bodies and their partial overlap with lysosomes of the stromal macrophages. ( A–C ) βIII + stromal nerve trunks expressed TRPV1. Intracellular TRPV1 staining was present in the macrophages that were in various depths of the stroma (color-coded depth projection, anterior: blue/purple; posterior: yellow) ( B , arrowheads; C , asterisks). ( D ) Absence of TRPV1 staining in tissues treated with peptide control antigen alongside positive staining of stromal CD45 + cells (red). ( E1–4 ) High resolution image of TRPV1 staining within the CD45 + stromal macrophages. ( F1–4 ) TRPV1 partially overlapped with the macrophage intracellular compartment that expresses CD68. ( G ) Orthogonal view of a CD45 + TRPV1 + stromal macrophage displaying the nonneuronal TRPV1 localization in both XY and XZ planes of the z-stack. ( H ) Orthogonal view of a CD68 + TRPV1 + stromal macrophage shows the overlap between lysosomes and TRPV1 proteins. Scale bars represent 100 µm ( A – D ) and 10 µm (E1 – 4, F1 – 4, G, H).

Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

Techniques: Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

doi: 10.1167/iovs.62.9.6

Figure Lengend Snippet: Epithelial TRPV1 + nerves and resident dendritic cells (DCs) under steady state conditions. ( A ) Representative images from the peripheral corneal epithelium showing resident intraepithelial CD45 + DCs in close proximity to TRPV1 + axons (asterisk), while some DCs did not appear to associate with TRPV1 + axons (arrowheads). ( B ) Orthogonal view of an intraepithelial CD45 + DC highlights the contacts between the TRPV1 + axons and DC in XYZ planes. ( C, D ) The absence of TRPV1 nerve staining in the peptide control validates the positive corneal nerve staining for TRPV1. ( E–H ) The resident DCs were immunopositive for CD11c and MHC class II. Scale bars represent 50 µm ( A , C , D ) and 10 µm ( E , F , G , H ).

Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

Techniques: Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

doi: 10.1167/iovs.62.9.6

Figure Lengend Snippet: Intraepithelial CD45 + dendriform cells in intact and injured corneas. ( A–D ) βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerve populations and their anatomical associations with DCs in the central and peripheral epithelium of intact ( A , B ) and injured corneas ( C , D ). ( E–L ) 3D image reconstructions show DC-nerve contacts between epithelial CD45 + DCs and βIII + TRPV1 + SBNP nerve axons (green: βIII; white: TRPV1). ( G–H ) DC-nerve complexes between DC and nerve axons (white area: βIII; yellow: TRPV1) were found within the DC dendrite. ( I–L ) One week after the epithelial abrasion, the degree of neuroimmune contacts between the DCs and nerve axons was similar to that observed in intact corneas (white area: βIII; blue: TRPV1) and DC dendrites. ( M, N ) The percentage of TRPV1 + nerve-associated DC was significantly higher across both central and peripheral corneas in the injured group compared to the intact control corneas ( P

Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

Techniques: Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

doi: 10.1167/iovs.62.9.6

Figure Lengend Snippet: Topographical distribution of TRPV1 + nerves in the intact mouse cornea. ( A–L ) Distribution of βIII + ( A , D , G , and J ) and TRPV1 + ( B , E , H , and K ) superficial nerve terminals (SNT) and subbasal nerve plexus (SBNP) in the central ( A–F ) and peripheral corneal epithelium ( G – L ). False colored merged images demonstrate the relationship between βIII + TRPV1 + (cyan) and βIII + TRPV1 − (magenta) nerve fibers ( C , F , I , and L ). ( M – P ) The sum length of TRPV1 + SNT was lower than TRPV1 − SNT in the central cornea ( P

Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

Techniques:

Journal: Investigative Ophthalmology & Visual Science

Article Title: Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury

doi: 10.1167/iovs.62.9.6

Figure Lengend Snippet: Stromal TRPV1 + nerves and CD45 + TRPV1 + macrophages in intact and injured corneas. ( A–D ) Superimposed images of βIII, TRPV1, and CD45 staining demonstrate TRPV1 + and TRPV1 − nerves and their anatomical interaction with macrophages in central and peripheral stroma of intact ( A , B ) and injured corneas ( C , D ). ( E–H ) The sum lengths of TRPV1 + stromal nerves were higher in both central and peripheral regions of the intact corneas ( P

Article Snippet: To confirm TRPV1 immunoreactivity, TRPV1 antibody was preadsorbed with the manufacturer's peptide control antigen (Cat #ACC030, Alomone labs) at 0.1 to 1 µg/ml, before immunostaining as above.

Techniques: Staining