antibodiesa rabbit polyclonal human anti trpc6 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs antibodiesa rabbit polyclonal human anti trpc6 antibody
    Nephrin clustering affects the distribution of <t>TRPC6</t> in HEK293 cells and potentially FFA induced changes in [Ca 2+ ] i . HEK293 were transiently transfected with TRPC6 and CD16-nephrin for 24 h. Cells were incubated with either secondary antibody alone (Ai–iii) or 1 mg/ml anti-CD16, then secondary antibody (Bi–iii) before fixing and immunostaining for TRPC6. Dapi nuclear staining is also shown in the merged images. Transfected HEKs, loaded with Fura-2AM were incubated in 1 mg/ml anti-CD16 for 10 min or vehicle, then incubated with 1 mg/ml mouse IgG for 10 min and finally stimulated with 200 μM FFA. Representative traces are shown for cells stimulated with FFA that were transfected with TRPC6 and pcDNA3 or TRPC6 and CD16-nephrin under control and experimental conditions (C). Data from C are summarised in (D) as area under the curve ( p
    Antibodiesa Rabbit Polyclonal Human Anti Trpc6 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodiesa rabbit polyclonal human anti trpc6 antibody/product/Alomone Labs
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    antibodiesa rabbit polyclonal human anti trpc6 antibody - by Bioz Stars, 2022-08
    92/100 stars

    Images

    1) Product Images from "Functional distinctions in cytosolic calcium regulation between cells of the glomerular filtration barrier"

    Article Title: Functional distinctions in cytosolic calcium regulation between cells of the glomerular filtration barrier

    Journal: Cell Calcium

    doi: 10.1016/j.ceca.2010.06.005

    Nephrin clustering affects the distribution of TRPC6 in HEK293 cells and potentially FFA induced changes in [Ca 2+ ] i . HEK293 were transiently transfected with TRPC6 and CD16-nephrin for 24 h. Cells were incubated with either secondary antibody alone (Ai–iii) or 1 mg/ml anti-CD16, then secondary antibody (Bi–iii) before fixing and immunostaining for TRPC6. Dapi nuclear staining is also shown in the merged images. Transfected HEKs, loaded with Fura-2AM were incubated in 1 mg/ml anti-CD16 for 10 min or vehicle, then incubated with 1 mg/ml mouse IgG for 10 min and finally stimulated with 200 μM FFA. Representative traces are shown for cells stimulated with FFA that were transfected with TRPC6 and pcDNA3 or TRPC6 and CD16-nephrin under control and experimental conditions (C). Data from C are summarised in (D) as area under the curve ( p
    Figure Legend Snippet: Nephrin clustering affects the distribution of TRPC6 in HEK293 cells and potentially FFA induced changes in [Ca 2+ ] i . HEK293 were transiently transfected with TRPC6 and CD16-nephrin for 24 h. Cells were incubated with either secondary antibody alone (Ai–iii) or 1 mg/ml anti-CD16, then secondary antibody (Bi–iii) before fixing and immunostaining for TRPC6. Dapi nuclear staining is also shown in the merged images. Transfected HEKs, loaded with Fura-2AM were incubated in 1 mg/ml anti-CD16 for 10 min or vehicle, then incubated with 1 mg/ml mouse IgG for 10 min and finally stimulated with 200 μM FFA. Representative traces are shown for cells stimulated with FFA that were transfected with TRPC6 and pcDNA3 or TRPC6 and CD16-nephrin under control and experimental conditions (C). Data from C are summarised in (D) as area under the curve ( p

    Techniques Used: Transfection, Incubation, Immunostaining, Staining

    Expression and distribution of TRPC6 in ciGEnC and ciPod compared to a platelet positive control. Lysates from ciPod and cGEnC were Western blotted and probed using anti-TRPC6 and anti-actin antibodies. (A) Representative images of ciGEnC (Bi) and ciPod (Bii) were microinjected with pcDNA3TRPC6 and immunofluorescence was carried out using an anti-TRPC6 antibody. Cells were imaged using confocal microscopy. X–Y stacks of ciGEnC and ciPod in (B) are shown in (Ci) and (Cii) respectively.
    Figure Legend Snippet: Expression and distribution of TRPC6 in ciGEnC and ciPod compared to a platelet positive control. Lysates from ciPod and cGEnC were Western blotted and probed using anti-TRPC6 and anti-actin antibodies. (A) Representative images of ciGEnC (Bi) and ciPod (Bii) were microinjected with pcDNA3TRPC6 and immunofluorescence was carried out using an anti-TRPC6 antibody. Cells were imaged using confocal microscopy. X–Y stacks of ciGEnC and ciPod in (B) are shown in (Ci) and (Cii) respectively.

    Techniques Used: Expressing, Positive Control, Western Blot, Immunofluorescence, Confocal Microscopy

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    Alomone Labs antibodiesa rabbit polyclonal human anti trpc6 antibody
    Nephrin clustering affects the distribution of <t>TRPC6</t> in HEK293 cells and potentially FFA induced changes in [Ca 2+ ] i . HEK293 were transiently transfected with TRPC6 and CD16-nephrin for 24 h. Cells were incubated with either secondary antibody alone (Ai–iii) or 1 mg/ml anti-CD16, then secondary antibody (Bi–iii) before fixing and immunostaining for TRPC6. Dapi nuclear staining is also shown in the merged images. Transfected HEKs, loaded with Fura-2AM were incubated in 1 mg/ml anti-CD16 for 10 min or vehicle, then incubated with 1 mg/ml mouse IgG for 10 min and finally stimulated with 200 μM FFA. Representative traces are shown for cells stimulated with FFA that were transfected with TRPC6 and pcDNA3 or TRPC6 and CD16-nephrin under control and experimental conditions (C). Data from C are summarised in (D) as area under the curve ( p
    Antibodiesa Rabbit Polyclonal Human Anti Trpc6 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodiesa rabbit polyclonal human anti trpc6 antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodiesa rabbit polyclonal human anti trpc6 antibody - by Bioz Stars, 2022-08
    92/100 stars
      Buy from Supplier

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    Nephrin clustering affects the distribution of TRPC6 in HEK293 cells and potentially FFA induced changes in [Ca 2+ ] i . HEK293 were transiently transfected with TRPC6 and CD16-nephrin for 24 h. Cells were incubated with either secondary antibody alone (Ai–iii) or 1 mg/ml anti-CD16, then secondary antibody (Bi–iii) before fixing and immunostaining for TRPC6. Dapi nuclear staining is also shown in the merged images. Transfected HEKs, loaded with Fura-2AM were incubated in 1 mg/ml anti-CD16 for 10 min or vehicle, then incubated with 1 mg/ml mouse IgG for 10 min and finally stimulated with 200 μM FFA. Representative traces are shown for cells stimulated with FFA that were transfected with TRPC6 and pcDNA3 or TRPC6 and CD16-nephrin under control and experimental conditions (C). Data from C are summarised in (D) as area under the curve ( p

    Journal: Cell Calcium

    Article Title: Functional distinctions in cytosolic calcium regulation between cells of the glomerular filtration barrier

    doi: 10.1016/j.ceca.2010.06.005

    Figure Lengend Snippet: Nephrin clustering affects the distribution of TRPC6 in HEK293 cells and potentially FFA induced changes in [Ca 2+ ] i . HEK293 were transiently transfected with TRPC6 and CD16-nephrin for 24 h. Cells were incubated with either secondary antibody alone (Ai–iii) or 1 mg/ml anti-CD16, then secondary antibody (Bi–iii) before fixing and immunostaining for TRPC6. Dapi nuclear staining is also shown in the merged images. Transfected HEKs, loaded with Fura-2AM were incubated in 1 mg/ml anti-CD16 for 10 min or vehicle, then incubated with 1 mg/ml mouse IgG for 10 min and finally stimulated with 200 μM FFA. Representative traces are shown for cells stimulated with FFA that were transfected with TRPC6 and pcDNA3 or TRPC6 and CD16-nephrin under control and experimental conditions (C). Data from C are summarised in (D) as area under the curve ( p

    Article Snippet: 2.2 AntibodiesA rabbit polyclonal human anti-TRPC6 antibody (Alomone, Jerusalem, Israel) was used for immunofluorescence and Western blotting.

    Techniques: Transfection, Incubation, Immunostaining, Staining

    Expression and distribution of TRPC6 in ciGEnC and ciPod compared to a platelet positive control. Lysates from ciPod and cGEnC were Western blotted and probed using anti-TRPC6 and anti-actin antibodies. (A) Representative images of ciGEnC (Bi) and ciPod (Bii) were microinjected with pcDNA3TRPC6 and immunofluorescence was carried out using an anti-TRPC6 antibody. Cells were imaged using confocal microscopy. X–Y stacks of ciGEnC and ciPod in (B) are shown in (Ci) and (Cii) respectively.

    Journal: Cell Calcium

    Article Title: Functional distinctions in cytosolic calcium regulation between cells of the glomerular filtration barrier

    doi: 10.1016/j.ceca.2010.06.005

    Figure Lengend Snippet: Expression and distribution of TRPC6 in ciGEnC and ciPod compared to a platelet positive control. Lysates from ciPod and cGEnC were Western blotted and probed using anti-TRPC6 and anti-actin antibodies. (A) Representative images of ciGEnC (Bi) and ciPod (Bii) were microinjected with pcDNA3TRPC6 and immunofluorescence was carried out using an anti-TRPC6 antibody. Cells were imaged using confocal microscopy. X–Y stacks of ciGEnC and ciPod in (B) are shown in (Ci) and (Cii) respectively.

    Article Snippet: 2.2 AntibodiesA rabbit polyclonal human anti-TRPC6 antibody (Alomone, Jerusalem, Israel) was used for immunofluorescence and Western blotting.

    Techniques: Expressing, Positive Control, Western Blot, Immunofluorescence, Confocal Microscopy