cacna1  (Alomone Labs)


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    Structured Review

    Alomone Labs cacna1
    Cacna1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cacna1 - by Bioz Stars, 2022-07
    92/100 stars

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    Alomone Labs cav1 2
    Increased immunolabeling for <t>Cav1.2</t> in Kv2.1 KO brain sections, and reduced spark frequency in cultured Kv2.1 KO CHNs. (A) Column shows exemplar images of the hippocampus acquired from brain sections of adult WT mice immunolabeled for Kv2.2 (red), Cav1.2 (green) and Kv4.2 (blue) (scale bar: 200 μm). (B) As in A but acquired from Kv2.1 KO mice. (C) Summary graphs of normalized mean fluorescence intensity of Kv2.2, Kv4.2, and Cav1.2 immunolabeling from ROIs from various laminae within CA1 (s.p.: stratum pyramidale ; s.r.: stratum radiatum ) and DG (s.g.: stratum granulosum; mo: molecular layer) in brain sections from adult WT (red) and Kv2.1 KO (black) mice. Each point corresponds to an individual mouse (Cav1.2 vs. Kv2.2: *p=0.0408; Cav1.2 vs. Kv4.2: **p=0.0018, ***p=0.0007). (D) A single optical section image of a WT mouse CHN immunolabeled for Kv2.1, Cav1.2, and RyRs (scale bar: 10 μm). (E) As in D but acquired from a Kv2.1 KO mouse CHN. (F) Representative WT mouse CHN loaded with Cal590 and imaged with TIRF microscopy, followed by post-hoc immunolabeling for RyRs, Kv2.1, and Cav1.2. Arrows indicate ROIs where spontaneous Ca 2+ signals were detected; dashed circles indicate approximate regions where immunolabeling for Kv2.1, Cav1.2, and RyRs was detectable. Kymograph showing the localized Ca 2+ release events detected at ROIs are depicted to the right. (G) Summary data of the amplitude, frequency and spatial spread (width) of all sparks recorded from WT and Kv2.1 KO mouse CHNs. Each point corresponds to a single cell (***p=0.0042 versus WT; Student’s t -test).
    Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav1 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cav1 2 - by Bioz Stars, 2022-07
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    92
    Alomone Labs anti human cav1 2 cacna1c antibody
    Increased immunolabeling for <t>Cav1.2</t> in Kv2.1 KO brain sections, and reduced spark frequency in cultured Kv2.1 KO CHNs. (A) Column shows exemplar images of the hippocampus acquired from brain sections of adult WT mice immunolabeled for Kv2.2 (red), Cav1.2 (green) and Kv4.2 (blue) (scale bar: 200 μm). (B) As in A but acquired from Kv2.1 KO mice. (C) Summary graphs of normalized mean fluorescence intensity of Kv2.2, Kv4.2, and Cav1.2 immunolabeling from ROIs from various laminae within CA1 (s.p.: stratum pyramidale ; s.r.: stratum radiatum ) and DG (s.g.: stratum granulosum; mo: molecular layer) in brain sections from adult WT (red) and Kv2.1 KO (black) mice. Each point corresponds to an individual mouse (Cav1.2 vs. Kv2.2: *p=0.0408; Cav1.2 vs. Kv4.2: **p=0.0018, ***p=0.0007). (D) A single optical section image of a WT mouse CHN immunolabeled for Kv2.1, Cav1.2, and RyRs (scale bar: 10 μm). (E) As in D but acquired from a Kv2.1 KO mouse CHN. (F) Representative WT mouse CHN loaded with Cal590 and imaged with TIRF microscopy, followed by post-hoc immunolabeling for RyRs, Kv2.1, and Cav1.2. Arrows indicate ROIs where spontaneous Ca 2+ signals were detected; dashed circles indicate approximate regions where immunolabeling for Kv2.1, Cav1.2, and RyRs was detectable. Kymograph showing the localized Ca 2+ release events detected at ROIs are depicted to the right. (G) Summary data of the amplitude, frequency and spatial spread (width) of all sparks recorded from WT and Kv2.1 KO mouse CHNs. Each point corresponds to a single cell (***p=0.0042 versus WT; Student’s t -test).
    Anti Human Cav1 2 Cacna1c Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human cav1 2 cacna1c antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
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    anti human cav1 2 cacna1c antibody - by Bioz Stars, 2022-07
    92/100 stars
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    Increased immunolabeling for Cav1.2 in Kv2.1 KO brain sections, and reduced spark frequency in cultured Kv2.1 KO CHNs. (A) Column shows exemplar images of the hippocampus acquired from brain sections of adult WT mice immunolabeled for Kv2.2 (red), Cav1.2 (green) and Kv4.2 (blue) (scale bar: 200 μm). (B) As in A but acquired from Kv2.1 KO mice. (C) Summary graphs of normalized mean fluorescence intensity of Kv2.2, Kv4.2, and Cav1.2 immunolabeling from ROIs from various laminae within CA1 (s.p.: stratum pyramidale ; s.r.: stratum radiatum ) and DG (s.g.: stratum granulosum; mo: molecular layer) in brain sections from adult WT (red) and Kv2.1 KO (black) mice. Each point corresponds to an individual mouse (Cav1.2 vs. Kv2.2: *p=0.0408; Cav1.2 vs. Kv4.2: **p=0.0018, ***p=0.0007). (D) A single optical section image of a WT mouse CHN immunolabeled for Kv2.1, Cav1.2, and RyRs (scale bar: 10 μm). (E) As in D but acquired from a Kv2.1 KO mouse CHN. (F) Representative WT mouse CHN loaded with Cal590 and imaged with TIRF microscopy, followed by post-hoc immunolabeling for RyRs, Kv2.1, and Cav1.2. Arrows indicate ROIs where spontaneous Ca 2+ signals were detected; dashed circles indicate approximate regions where immunolabeling for Kv2.1, Cav1.2, and RyRs was detectable. Kymograph showing the localized Ca 2+ release events detected at ROIs are depicted to the right. (G) Summary data of the amplitude, frequency and spatial spread (width) of all sparks recorded from WT and Kv2.1 KO mouse CHNs. Each point corresponds to a single cell (***p=0.0042 versus WT; Student’s t -test).

    Journal: bioRxiv

    Article Title: Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in neurons

    doi: 10.1101/702514

    Figure Lengend Snippet: Increased immunolabeling for Cav1.2 in Kv2.1 KO brain sections, and reduced spark frequency in cultured Kv2.1 KO CHNs. (A) Column shows exemplar images of the hippocampus acquired from brain sections of adult WT mice immunolabeled for Kv2.2 (red), Cav1.2 (green) and Kv4.2 (blue) (scale bar: 200 μm). (B) As in A but acquired from Kv2.1 KO mice. (C) Summary graphs of normalized mean fluorescence intensity of Kv2.2, Kv4.2, and Cav1.2 immunolabeling from ROIs from various laminae within CA1 (s.p.: stratum pyramidale ; s.r.: stratum radiatum ) and DG (s.g.: stratum granulosum; mo: molecular layer) in brain sections from adult WT (red) and Kv2.1 KO (black) mice. Each point corresponds to an individual mouse (Cav1.2 vs. Kv2.2: *p=0.0408; Cav1.2 vs. Kv4.2: **p=0.0018, ***p=0.0007). (D) A single optical section image of a WT mouse CHN immunolabeled for Kv2.1, Cav1.2, and RyRs (scale bar: 10 μm). (E) As in D but acquired from a Kv2.1 KO mouse CHN. (F) Representative WT mouse CHN loaded with Cal590 and imaged with TIRF microscopy, followed by post-hoc immunolabeling for RyRs, Kv2.1, and Cav1.2. Arrows indicate ROIs where spontaneous Ca 2+ signals were detected; dashed circles indicate approximate regions where immunolabeling for Kv2.1, Cav1.2, and RyRs was detectable. Kymograph showing the localized Ca 2+ release events detected at ROIs are depicted to the right. (G) Summary data of the amplitude, frequency and spatial spread (width) of all sparks recorded from WT and Kv2.1 KO mouse CHNs. Each point corresponds to a single cell (***p=0.0042 versus WT; Student’s t -test).

    Article Snippet: To isolate gating currents and I tail produced by Cav1.2, we subtracted currents measured in the presence of nitrendipine from those measured in KRB alone.

    Techniques: Immunolabeling, Cell Culture, Mouse Assay, Fluorescence, Microscopy

    LTCCs are recruited to Kv2-induced EPJs. (A) Upper row: TIRF images of a HEK293T cell cotransfected with GFP-Cav1.2 (green), BFP-SEC61β (blue) and LTCC auxiliary subunits Cavβ3 and Cavα 2 δ 1 (not shown) and without Kv2.1 (scale bar: 10 μm). Lower row: as in upper row, but in a cell additionally cotransfected with DsRed-Kv2.1. (B) Summary graphs of Cav1.2 cluster size (left panel), the cluster size frequency distribution (center panel), and a scatterplot of paired measurements of Kv2.1 and Cav1.2 cluster sizes (left panel) measured from HEK293T cells transfected with GFP-Cav1.2, Cavβ3, and Cavα 2 δ 1 alone (black) or additionally cotransfected with DsRed-Kv2.1 (red) Bars are mean ±SD (****p

    Journal: bioRxiv

    Article Title: Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in neurons

    doi: 10.1101/702514

    Figure Lengend Snippet: LTCCs are recruited to Kv2-induced EPJs. (A) Upper row: TIRF images of a HEK293T cell cotransfected with GFP-Cav1.2 (green), BFP-SEC61β (blue) and LTCC auxiliary subunits Cavβ3 and Cavα 2 δ 1 (not shown) and without Kv2.1 (scale bar: 10 μm). Lower row: as in upper row, but in a cell additionally cotransfected with DsRed-Kv2.1. (B) Summary graphs of Cav1.2 cluster size (left panel), the cluster size frequency distribution (center panel), and a scatterplot of paired measurements of Kv2.1 and Cav1.2 cluster sizes (left panel) measured from HEK293T cells transfected with GFP-Cav1.2, Cavβ3, and Cavα 2 δ 1 alone (black) or additionally cotransfected with DsRed-Kv2.1 (red) Bars are mean ±SD (****p

    Article Snippet: To isolate gating currents and I tail produced by Cav1.2, we subtracted currents measured in the presence of nitrendipine from those measured in KRB alone.

    Techniques: Transfection

    Cav1.2 channel activity is increased in cells coexpressing Stac1 upon coexpression with Kv2.1 P404W . A-C: Data recorded from HEK293T cells transfected with Cav1.2-GFP and auxiliary subunits Cavβ3, Cavα 2 δ 1 , and STAC1, without (+pCDNA3, in black) or with Kv2.1 P404W (in red). (A) Representative Ca 2+ current traces at +10 mV. (B) Normalized I-V relationship of whole-cell I Ca recorded from n =8 (Cav1.2 + pcDNA3) and n =9 (Cav1.2 + Kv2.1 P404W ) cells. (C) Voltage-dependence of whole cell Cav1.2 conductance G / G max . For the conductance-voltage relationships, the half-maximal activation voltage V 1/2 =1.6±2.0 [pcDNA3] vs. -9.5±2.9 [+Kv2.1 P404W ] mV, p=0.0166; slope factor k=8.8±1.2 [pcDNA3] vs. 6.1±0.6 [+Kv2.1 P404W ], p=0.0490; Student’s t -test).

    Journal: bioRxiv

    Article Title: Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in neurons

    doi: 10.1101/702514

    Figure Lengend Snippet: Cav1.2 channel activity is increased in cells coexpressing Stac1 upon coexpression with Kv2.1 P404W . A-C: Data recorded from HEK293T cells transfected with Cav1.2-GFP and auxiliary subunits Cavβ3, Cavα 2 δ 1 , and STAC1, without (+pCDNA3, in black) or with Kv2.1 P404W (in red). (A) Representative Ca 2+ current traces at +10 mV. (B) Normalized I-V relationship of whole-cell I Ca recorded from n =8 (Cav1.2 + pcDNA3) and n =9 (Cav1.2 + Kv2.1 P404W ) cells. (C) Voltage-dependence of whole cell Cav1.2 conductance G / G max . For the conductance-voltage relationships, the half-maximal activation voltage V 1/2 =1.6±2.0 [pcDNA3] vs. -9.5±2.9 [+Kv2.1 P404W ] mV, p=0.0166; slope factor k=8.8±1.2 [pcDNA3] vs. 6.1±0.6 [+Kv2.1 P404W ], p=0.0490; Student’s t -test).

    Article Snippet: To isolate gating currents and I tail produced by Cav1.2, we subtracted currents measured in the presence of nitrendipine from those measured in KRB alone.

    Techniques: Activity Assay, Transfection, Activation Assay

    Kv2.1 spatially associates with Cav1.2 and RyRs in brain neurons. (A) Single optical section image of a rat CHN immunolabeled for PSD-95, Cav1.2, and MAP2 (scale bar: 20 μm). Note large population of somatic Cav1.2 channels distant from excitatory synapses located primarily on more distal dendrites. Inset of merged panel shows expanded view of dendritic PSD-95 and Cav1.2 immunolabeling marked by box (inset scale bar: 5 μm). (B) Single confocal optical section of the soma of rat CHN immunolabeled for Kv2.1, Cav1.2, and RyRs (scale bar: 5 μm). The row of panels below the main panels shows an expanded view of somatic immunolabeling in the region marked by the box in the main panels; arrows indicate selected regions of colocalized Kv2.1, Cav1.2, and RyR immunolabeling (inset scale bar: 1 μm). (C) As in B, but in a CHN displaying more prominent colocalization of clustered Kv2.1, Cav1.2, and RyRs. (D) Super resolution (N-SIM) image of the basal membrane of the soma of a rat CHN immunolabeled for Kv2.1, Cav1.2, and Cav1.3 (scale bar: 5 μm). (E) Expanded view of the boxed region in the merged image of D (scale bar: 1.25 μm). (F) Super resolution (N-SIM) image of the basal membrane of the soma of a rat CHN immunolabeled for Cav1.3 and RyRs (scale bar: 5 μm). Inset in merged panel shows a higher magnification view of the boxed area (inset scale bar: 0.625 μm). (G) Panels show exemplar images of the hippocampus acquired from a brain section from an adult rat immunolabeled for Kv2.1 (red), Cav1.2, (green) and RyRs (blue), and the merged image (scale bar: 200 μm). (H) As in G, but acquired from an adult mouse brain section. (I) Confocal optical section obtained from the dentate gyrus of a rat brain section immunolabeled for Kv2.1 (red) and Cav1.2 (green) (scale bar: 10 μm). The row below the main panels shows expanded views of immunolabeling in the region marked by the box in the main panels; arrowheads indicate region selected for intensity profile line scan (scale bar: 2 μm). Line scan obtained from inset is shown to the right. (J) Confocal optical section obtained from the pyramidal cell layer of hippocampal area CA1 in a rat brain section immunolabeled for Kv2.1 (red), Cav1.2 (green), and RyRs (blue) (scale bar: 10 μm). The row below the main panels shows expanded view of immunolabeling in the region marked by the box in the main panels (scale bar: 2 μm). (K) As in I but acquired from a mouse brain section. (L) As in J but acquired from a mouse brain section.

    Journal: bioRxiv

    Article Title: Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in neurons

    doi: 10.1101/702514

    Figure Lengend Snippet: Kv2.1 spatially associates with Cav1.2 and RyRs in brain neurons. (A) Single optical section image of a rat CHN immunolabeled for PSD-95, Cav1.2, and MAP2 (scale bar: 20 μm). Note large population of somatic Cav1.2 channels distant from excitatory synapses located primarily on more distal dendrites. Inset of merged panel shows expanded view of dendritic PSD-95 and Cav1.2 immunolabeling marked by box (inset scale bar: 5 μm). (B) Single confocal optical section of the soma of rat CHN immunolabeled for Kv2.1, Cav1.2, and RyRs (scale bar: 5 μm). The row of panels below the main panels shows an expanded view of somatic immunolabeling in the region marked by the box in the main panels; arrows indicate selected regions of colocalized Kv2.1, Cav1.2, and RyR immunolabeling (inset scale bar: 1 μm). (C) As in B, but in a CHN displaying more prominent colocalization of clustered Kv2.1, Cav1.2, and RyRs. (D) Super resolution (N-SIM) image of the basal membrane of the soma of a rat CHN immunolabeled for Kv2.1, Cav1.2, and Cav1.3 (scale bar: 5 μm). (E) Expanded view of the boxed region in the merged image of D (scale bar: 1.25 μm). (F) Super resolution (N-SIM) image of the basal membrane of the soma of a rat CHN immunolabeled for Cav1.3 and RyRs (scale bar: 5 μm). Inset in merged panel shows a higher magnification view of the boxed area (inset scale bar: 0.625 μm). (G) Panels show exemplar images of the hippocampus acquired from a brain section from an adult rat immunolabeled for Kv2.1 (red), Cav1.2, (green) and RyRs (blue), and the merged image (scale bar: 200 μm). (H) As in G, but acquired from an adult mouse brain section. (I) Confocal optical section obtained from the dentate gyrus of a rat brain section immunolabeled for Kv2.1 (red) and Cav1.2 (green) (scale bar: 10 μm). The row below the main panels shows expanded views of immunolabeling in the region marked by the box in the main panels; arrowheads indicate region selected for intensity profile line scan (scale bar: 2 μm). Line scan obtained from inset is shown to the right. (J) Confocal optical section obtained from the pyramidal cell layer of hippocampal area CA1 in a rat brain section immunolabeled for Kv2.1 (red), Cav1.2 (green), and RyRs (blue) (scale bar: 10 μm). The row below the main panels shows expanded view of immunolabeling in the region marked by the box in the main panels (scale bar: 2 μm). (K) As in I but acquired from a mouse brain section. (L) As in J but acquired from a mouse brain section.

    Article Snippet: To isolate gating currents and I tail produced by Cav1.2, we subtracted currents measured in the presence of nitrendipine from those measured in KRB alone.

    Techniques: Immunolabeling

    Cav1.2 channel activity is increased by coexpression with Kv2.1 P404W . (A) Representative Ca 2+ current trace families recorded from HEK293T cells transfected with Cav1.2-GFP and auxiliary subunits Cavβ3 and Cavα 2 δ 1 , without (+ pcDNA3 empty vector) with cotransfection of DsRed-Kv2.1 P404W . For panels D-F, data are from cells without (+ pcDNA3 empty vector, in black) or with coexpression of Kv2.1 P404W (in red). (B) Normalized current-voltage ( I-V ) relationship of whole-cell I Ca recorded from n=17 (Cav1.2 + pcDNA3) and n =10 (Cav1.2 + Kv2.1 P404W ) cells. (C) Voltage-dependence of whole cell Cav1.2 conductance G / G max and steady-state inactivation I / I max . For the conductance-voltage relationships, the half-maximal activation voltage V 1/2 =-8.9±0.8 [pcDNA3] vs. -13.9±1.6 [+Kv2.1 P404W ] mV, p=0.0045; slope factor k =6.9±0.3 [pcDNA3] vs. 4.5±0.7 [+Kv2.1 P404W ], p=0.0025; Student’s t-test. (D) Representative nitrendipine-sensitive Cav1.2 gating and tail currents recorded from control (pcDNA3) cells and cells coexpressing Kv2.1 P404W . (E) Quantification of nitrendipine-sensitive Cav1.2 Q on (left), I tail (center), and Q on vs. I tail (right).. Each point corresponds to a single cell (*p=0.019, Student’s t -test). (F) Average Rhod-2 fluorescence intensity measurements obtained from cells held at different membrane potentials during voltage clamp experiments ( n =4 cells per condition). (G) Average fluorescence intensity measurements from Fluo4-loaded HEK293T cells transfected with Cav1.2, auxiliary subunits Cavβ3 and Cavα2δ, without (+ pcDNA3 empty vector, in black) or with Cotransfection of Kv2.1 WT (in blue) or Kv2.1 P404W (in red). Ca 2+ influx was stimulated by depolarization with high extracellular K + (45 mM) as indicated on the graph. (H) Average peak fluorescence values obtained during high-K + depolarization of HEK293T cells expressing Cav1.2 and Kv2.1 WT or Kv2.1 P404W as in G. Each point corresponds to a single cell. Bars are mean ± SD (**p

    Journal: bioRxiv

    Article Title: Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in neurons

    doi: 10.1101/702514

    Figure Lengend Snippet: Cav1.2 channel activity is increased by coexpression with Kv2.1 P404W . (A) Representative Ca 2+ current trace families recorded from HEK293T cells transfected with Cav1.2-GFP and auxiliary subunits Cavβ3 and Cavα 2 δ 1 , without (+ pcDNA3 empty vector) with cotransfection of DsRed-Kv2.1 P404W . For panels D-F, data are from cells without (+ pcDNA3 empty vector, in black) or with coexpression of Kv2.1 P404W (in red). (B) Normalized current-voltage ( I-V ) relationship of whole-cell I Ca recorded from n=17 (Cav1.2 + pcDNA3) and n =10 (Cav1.2 + Kv2.1 P404W ) cells. (C) Voltage-dependence of whole cell Cav1.2 conductance G / G max and steady-state inactivation I / I max . For the conductance-voltage relationships, the half-maximal activation voltage V 1/2 =-8.9±0.8 [pcDNA3] vs. -13.9±1.6 [+Kv2.1 P404W ] mV, p=0.0045; slope factor k =6.9±0.3 [pcDNA3] vs. 4.5±0.7 [+Kv2.1 P404W ], p=0.0025; Student’s t-test. (D) Representative nitrendipine-sensitive Cav1.2 gating and tail currents recorded from control (pcDNA3) cells and cells coexpressing Kv2.1 P404W . (E) Quantification of nitrendipine-sensitive Cav1.2 Q on (left), I tail (center), and Q on vs. I tail (right).. Each point corresponds to a single cell (*p=0.019, Student’s t -test). (F) Average Rhod-2 fluorescence intensity measurements obtained from cells held at different membrane potentials during voltage clamp experiments ( n =4 cells per condition). (G) Average fluorescence intensity measurements from Fluo4-loaded HEK293T cells transfected with Cav1.2, auxiliary subunits Cavβ3 and Cavα2δ, without (+ pcDNA3 empty vector, in black) or with Cotransfection of Kv2.1 WT (in blue) or Kv2.1 P404W (in red). Ca 2+ influx was stimulated by depolarization with high extracellular K + (45 mM) as indicated on the graph. (H) Average peak fluorescence values obtained during high-K + depolarization of HEK293T cells expressing Cav1.2 and Kv2.1 WT or Kv2.1 P404W as in G. Each point corresponds to a single cell. Bars are mean ± SD (**p

    Article Snippet: To isolate gating currents and I tail produced by Cav1.2, we subtracted currents measured in the presence of nitrendipine from those measured in KRB alone.

    Techniques: Activity Assay, Transfection, Plasmid Preparation, Cotransfection, Activation Assay, Fluorescence, Expressing

    Protein expression levels of Cav1.2, Piezo1, CaM, and Src in human LAA tissues. (A) Representative western blots and densitometric analysis of Cav1.2 and Piezo1 proteins in LA tissues of AF patients and those with SR. (B) Representative western blots and densitometric analysis of CaM and Src protein in LA tissues of AF patients and those with SR. GAPDH was the internal control. ** p

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Piezo1 Participated in Decreased L-Type Calcium Current Induced by High Hydrostatic Pressure via. CaM/Src/Pitx2 Activation in Atrial Myocytes

    doi: 10.3389/fcvm.2022.842885

    Figure Lengend Snippet: Protein expression levels of Cav1.2, Piezo1, CaM, and Src in human LAA tissues. (A) Representative western blots and densitometric analysis of Cav1.2 and Piezo1 proteins in LA tissues of AF patients and those with SR. (B) Representative western blots and densitometric analysis of CaM and Src protein in LA tissues of AF patients and those with SR. GAPDH was the internal control. ** p

    Article Snippet: Samples were adjusted with loading buffer to attain equal protein volumes and heated at 55°C for 10 min (for Piezo1 and Cav1.2) or 100°C for 10 min (for Src and Pitx2) for denaturation.

    Techniques: Expressing, Chick Chorioallantoic Membrane Assay, Western Blot