itpr1 (Alomone Labs)


Structured Review

Itpr1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/itpr1/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Inositol 1,4,5-trisphosphate receptor type 1 autoantibodies in paraneoplastic and non-paraneoplastic peripheral neuropathy"
Article Title: Inositol 1,4,5-trisphosphate receptor type 1 autoantibodies in paraneoplastic and non-paraneoplastic peripheral neuropathy
Journal: Journal of Neuroinflammation
doi: 10.1186/s12974-016-0737-x

Figure Legend Snippet: Increase in the proportion of B cells, CD4 + T cells and CD8 + CD45RO + T cells among PBMCs from patient 1 (2 × 10 5 cells) following stimulation with ITPR1 (10 μg/ml) ( orange outlines ) compared with stimulation with GFAP (10 μg/ml) and compared with healthy donor PBMCs stimulated with the same amount of ITPR1 or GFAP, respectively
Techniques Used:
![Expression of ITPR1 in the spinal cord ( a , e ... Expression of ITPR1 in the spinal cord ( a , e – g ), sural nerve ( h ) and dorsal root ganglia (DRG) ( b – d , i – j ) of adult mouse ( a , d ), rat ( b – c , e – f , i – j ) and rhesus monkey ( g – h ) and as detected by in situ hybridization ( a , b , e ) and immunostaining ( c – d , f – j ), and binding of IgG from the patient’s CSF ( i ) and serum ( j ) to sensory neuron somata in rat DRG as detected by indirect immunofluorescence. A polyclonal rabbit antibody to ITPR1 (Dianova) and an AlexaFluor®568-labelled goat anti-rabbit IgG secondary antibody were used to detect ITPR1 expression ( red ). A fluorescein isothiocyanate-labelled goat anti-human IgG antibody was used to visualize bound patient IgG ( green ). Yellow areas in j indicate overlay of the patient’s IgG and the commercial ITPR1 antibody. a Taken from the Allen Spinal Cord Atlas [ 64 ] ( http://mousespinal.brain-map.org/ ). b , c and e Reproduced from Dent et al. [ 2 ] with kind permission of the publisher , d from Zhuang et al. [ 5 ] (made available under the Creative Commons CC0 public domain dedication; https://creativecommons.org/publicdomain/zero/1.0/ ) , and f from Sharp et al. [ 3 ] with kind permission of the publisher. DH dorsal horn, IP3R inositol-1,4,5 trisphosphate receptor](https://storage.googleapis.com/bioz_article_images/PMC5078930/12974_2016_737_Fig6_HTML.jpg)
Figure Legend Snippet: Expression of ITPR1 in the spinal cord ( a , e – g ), sural nerve ( h ) and dorsal root ganglia (DRG) ( b – d , i – j ) of adult mouse ( a , d ), rat ( b – c , e – f , i – j ) and rhesus monkey ( g – h ) and as detected by in situ hybridization ( a , b , e ) and immunostaining ( c – d , f – j ), and binding of IgG from the patient’s CSF ( i ) and serum ( j ) to sensory neuron somata in rat DRG as detected by indirect immunofluorescence. A polyclonal rabbit antibody to ITPR1 (Dianova) and an AlexaFluor®568-labelled goat anti-rabbit IgG secondary antibody were used to detect ITPR1 expression ( red ). A fluorescein isothiocyanate-labelled goat anti-human IgG antibody was used to visualize bound patient IgG ( green ). Yellow areas in j indicate overlay of the patient’s IgG and the commercial ITPR1 antibody. a Taken from the Allen Spinal Cord Atlas [ 64 ] ( http://mousespinal.brain-map.org/ ). b , c and e Reproduced from Dent et al. [ 2 ] with kind permission of the publisher , d from Zhuang et al. [ 5 ] (made available under the Creative Commons CC0 public domain dedication; https://creativecommons.org/publicdomain/zero/1.0/ ) , and f from Sharp et al. [ 3 ] with kind permission of the publisher. DH dorsal horn, IP3R inositol-1,4,5 trisphosphate receptor
Techniques Used: Expressing, In Situ Hybridization, Immunostaining, Binding Assay, Immunofluorescence

Figure Legend Snippet: ITPR1-specific antibody index
Techniques Used:

Figure Legend Snippet: ITPR1 is expressed in a subset of carcinomas of the lung. a Quantitative Western blot analysis showing ITPR1 expression as percentage of the ITPR1 expression in normal human bronchial epithelial (NHBE) cells, in the small cell lung cancer cell line H1339 and in the non-small cell lung cancer cell line HCC. The expression of IP3R was increased with H1339 showing the highest expression ( n = 4, * P
Techniques Used: Western Blot, Expressing
![ITPR1-IgG as detected by a immunohistochemistry, b a dot-blot ... ITPR1-IgG as detected by a immunohistochemistry, b a dot-blot assay and c , d a cell-based assay (exemplary findings). a Binding of patient serum IgG and of a commercial antibody to ITPR1 to formalin-fixed mouse (as well as rat and primate, not shown) cerebellum tissue sections ( MoL molecular layer, PCL purkinje cell layer). Binding of patient IgG is depicted in green (goat anti-human IgG Alexa Fluor® 488); binding of a commercial rabbit anti-ITPR1 antibody in red (goat anti-rabbit IgG Alexa Fluor® 568); yellow indicates binding of both antibodies. Cell nuclei were stained by 4',6-diamidino-2-phenylindole ( blue colour ). b Binding of IgG from the patient’s serum and from two previously reported ITPR1-IgG-positive patients [ 1 ] to purified native ITPR1 from rat brain in a dot-blot assay; by contrast, sera from four healthy and disease controls did not bind to ITPR1. A commercial goat anti-human IgG antibody labelled with IRdye®700 (Rockland, Limerick, PA) was used to detect bound patient IgG. c Binding of patient IgG to cells transfected with mouse ITPR1 ( left ) but not to mock-transfected HEK293 cells used as control substrate ( right ). Binding of patient IgG was visualized using a goat anti-human IgG secondary antibody labelled with fluorescein isothiocyanate. See the “ Methods ” section for details. ACA autoimmune cerebellar ataxia, PEX plasma exchange](https://storage.googleapis.com/bioz_article_images/PMC5078930/12974_2016_737_Fig1_HTML.jpg)
Figure Legend Snippet: ITPR1-IgG as detected by a immunohistochemistry, b a dot-blot assay and c , d a cell-based assay (exemplary findings). a Binding of patient serum IgG and of a commercial antibody to ITPR1 to formalin-fixed mouse (as well as rat and primate, not shown) cerebellum tissue sections ( MoL molecular layer, PCL purkinje cell layer). Binding of patient IgG is depicted in green (goat anti-human IgG Alexa Fluor® 488); binding of a commercial rabbit anti-ITPR1 antibody in red (goat anti-rabbit IgG Alexa Fluor® 568); yellow indicates binding of both antibodies. Cell nuclei were stained by 4',6-diamidino-2-phenylindole ( blue colour ). b Binding of IgG from the patient’s serum and from two previously reported ITPR1-IgG-positive patients [ 1 ] to purified native ITPR1 from rat brain in a dot-blot assay; by contrast, sera from four healthy and disease controls did not bind to ITPR1. A commercial goat anti-human IgG antibody labelled with IRdye®700 (Rockland, Limerick, PA) was used to detect bound patient IgG. c Binding of patient IgG to cells transfected with mouse ITPR1 ( left ) but not to mock-transfected HEK293 cells used as control substrate ( right ). Binding of patient IgG was visualized using a goat anti-human IgG secondary antibody labelled with fluorescein isothiocyanate. See the “ Methods ” section for details. ACA autoimmune cerebellar ataxia, PEX plasma exchange
Techniques Used: Immunohistochemistry, Dot Blot, Cell Based Assay, Binding Assay, Staining, Purification, Transfection

Figure Legend Snippet: ITPR1-IgG in the CSF. Perfect overlap of the patient IgG from a CSF sample (patient 1) with a commercial antibody to ITPR1 as detected by IHC on a murine cerebellum tissue section. Bound patient IgG is depicted in green (Alexa Fluor® 488) and binding of the commercial anti-ITPR1 antibody in red (Alexa Fluor® 568); yellow indicates areas stained by both antibodies. Cell nuclei were stained by 4',6-diamidino-2-phenylindole ( blue )
Techniques Used: Immunohistochemistry, Binding Assay, Staining

Figure Legend Snippet: 3 H-thymidine uptake (cpm; mean of three stimulations) in proliferating PBMCs from patient 1 and from a healthy control following stimulation with ITPR1 or GFAP, respectively. a Note the significantly higher 3 H-thymidine uptake in patient PBMCs stimulated with ITPR1 than in the healthy donor; by contrast, no difference was noted after stimulation with GFAP or with PBS. b After subtraction of non-antigen-specific, GFAP-induced proliferation, a difference (Δcpm) between patient and healthy control of more than 600 cpm or 340 % was noted following ITPR1 stimulation
Techniques Used:

Figure Legend Snippet: ITPR1 class and subclass analysis revealed IgG1 a and IgA ( b , inset) antibodies in patient 1 and IgG2 antibodies in patient 2 (as well as in patient 3, not shown) b
Techniques Used:
2) Product Images from "Inositol 1,4,5-trisphosphate receptor type 1 autoantibodies in paraneoplastic and non-paraneoplastic peripheral neuropathy"
Article Title: Inositol 1,4,5-trisphosphate receptor type 1 autoantibodies in paraneoplastic and non-paraneoplastic peripheral neuropathy
Journal: Journal of Neuroinflammation
doi: 10.1186/s12974-016-0737-x

Figure Legend Snippet: Increase in the proportion of B cells, CD4 + T cells and CD8 + CD45RO + T cells among PBMCs from patient 1 (2 × 10 5 cells) following stimulation with ITPR1 (10 μg/ml) ( orange outlines ) compared with stimulation with GFAP (10 μg/ml) and compared with healthy donor PBMCs stimulated with the same amount of ITPR1 or GFAP, respectively
Techniques Used:
![Expression of ITPR1 in the spinal cord ( a , e ... Expression of ITPR1 in the spinal cord ( a , e – g ), sural nerve ( h ) and dorsal root ganglia (DRG) ( b – d , i – j ) of adult mouse ( a , d ), rat ( b – c , e – f , i – j ) and rhesus monkey ( g – h ) and as detected by in situ hybridization ( a , b , e ) and immunostaining ( c – d , f – j ), and binding of IgG from the patient’s CSF ( i ) and serum ( j ) to sensory neuron somata in rat DRG as detected by indirect immunofluorescence. A polyclonal rabbit antibody to ITPR1 (Dianova) and an AlexaFluor®568-labelled goat anti-rabbit IgG secondary antibody were used to detect ITPR1 expression ( red ). A fluorescein isothiocyanate-labelled goat anti-human IgG antibody was used to visualize bound patient IgG ( green ). Yellow areas in j indicate overlay of the patient’s IgG and the commercial ITPR1 antibody. a Taken from the Allen Spinal Cord Atlas [ 64 ] ( http://mousespinal.brain-map.org/ ). b , c and e Reproduced from Dent et al. [ 2 ] with kind permission of the publisher , d from Zhuang et al. [ 5 ] (made available under the Creative Commons CC0 public domain dedication; https://creativecommons.org/publicdomain/zero/1.0/ ) , and f from Sharp et al. [ 3 ] with kind permission of the publisher. DH dorsal horn, IP3R inositol-1,4,5 trisphosphate receptor](https://storage.googleapis.com/bioz_article_images/PMC5078930/12974_2016_737_Fig6_HTML.jpg)
Figure Legend Snippet: Expression of ITPR1 in the spinal cord ( a , e – g ), sural nerve ( h ) and dorsal root ganglia (DRG) ( b – d , i – j ) of adult mouse ( a , d ), rat ( b – c , e – f , i – j ) and rhesus monkey ( g – h ) and as detected by in situ hybridization ( a , b , e ) and immunostaining ( c – d , f – j ), and binding of IgG from the patient’s CSF ( i ) and serum ( j ) to sensory neuron somata in rat DRG as detected by indirect immunofluorescence. A polyclonal rabbit antibody to ITPR1 (Dianova) and an AlexaFluor®568-labelled goat anti-rabbit IgG secondary antibody were used to detect ITPR1 expression ( red ). A fluorescein isothiocyanate-labelled goat anti-human IgG antibody was used to visualize bound patient IgG ( green ). Yellow areas in j indicate overlay of the patient’s IgG and the commercial ITPR1 antibody. a Taken from the Allen Spinal Cord Atlas [ 64 ] ( http://mousespinal.brain-map.org/ ). b , c and e Reproduced from Dent et al. [ 2 ] with kind permission of the publisher , d from Zhuang et al. [ 5 ] (made available under the Creative Commons CC0 public domain dedication; https://creativecommons.org/publicdomain/zero/1.0/ ) , and f from Sharp et al. [ 3 ] with kind permission of the publisher. DH dorsal horn, IP3R inositol-1,4,5 trisphosphate receptor
Techniques Used: Expressing, In Situ Hybridization, Immunostaining, Binding Assay, Immunofluorescence

Figure Legend Snippet: ITPR1-specific antibody index
Techniques Used:

Figure Legend Snippet: ITPR1 is expressed in a subset of carcinomas of the lung. a Quantitative Western blot analysis showing ITPR1 expression as percentage of the ITPR1 expression in normal human bronchial epithelial (NHBE) cells, in the small cell lung cancer cell line H1339 and in the non-small cell lung cancer cell line HCC. The expression of IP3R was increased with H1339 showing the highest expression ( n = 4, * P
Techniques Used: Western Blot, Expressing
![ITPR1-IgG as detected by a immunohistochemistry, b a dot-blot ... ITPR1-IgG as detected by a immunohistochemistry, b a dot-blot assay and c , d a cell-based assay (exemplary findings). a Binding of patient serum IgG and of a commercial antibody to ITPR1 to formalin-fixed mouse (as well as rat and primate, not shown) cerebellum tissue sections ( MoL molecular layer, PCL purkinje cell layer). Binding of patient IgG is depicted in green (goat anti-human IgG Alexa Fluor® 488); binding of a commercial rabbit anti-ITPR1 antibody in red (goat anti-rabbit IgG Alexa Fluor® 568); yellow indicates binding of both antibodies. Cell nuclei were stained by 4',6-diamidino-2-phenylindole ( blue colour ). b Binding of IgG from the patient’s serum and from two previously reported ITPR1-IgG-positive patients [ 1 ] to purified native ITPR1 from rat brain in a dot-blot assay; by contrast, sera from four healthy and disease controls did not bind to ITPR1. A commercial goat anti-human IgG antibody labelled with IRdye®700 (Rockland, Limerick, PA) was used to detect bound patient IgG. c Binding of patient IgG to cells transfected with mouse ITPR1 ( left ) but not to mock-transfected HEK293 cells used as control substrate ( right ). Binding of patient IgG was visualized using a goat anti-human IgG secondary antibody labelled with fluorescein isothiocyanate. See the “ Methods ” section for details. ACA autoimmune cerebellar ataxia, PEX plasma exchange](https://storage.googleapis.com/bioz_article_images/PMC5078930/12974_2016_737_Fig1_HTML.jpg)
Figure Legend Snippet: ITPR1-IgG as detected by a immunohistochemistry, b a dot-blot assay and c , d a cell-based assay (exemplary findings). a Binding of patient serum IgG and of a commercial antibody to ITPR1 to formalin-fixed mouse (as well as rat and primate, not shown) cerebellum tissue sections ( MoL molecular layer, PCL purkinje cell layer). Binding of patient IgG is depicted in green (goat anti-human IgG Alexa Fluor® 488); binding of a commercial rabbit anti-ITPR1 antibody in red (goat anti-rabbit IgG Alexa Fluor® 568); yellow indicates binding of both antibodies. Cell nuclei were stained by 4',6-diamidino-2-phenylindole ( blue colour ). b Binding of IgG from the patient’s serum and from two previously reported ITPR1-IgG-positive patients [ 1 ] to purified native ITPR1 from rat brain in a dot-blot assay; by contrast, sera from four healthy and disease controls did not bind to ITPR1. A commercial goat anti-human IgG antibody labelled with IRdye®700 (Rockland, Limerick, PA) was used to detect bound patient IgG. c Binding of patient IgG to cells transfected with mouse ITPR1 ( left ) but not to mock-transfected HEK293 cells used as control substrate ( right ). Binding of patient IgG was visualized using a goat anti-human IgG secondary antibody labelled with fluorescein isothiocyanate. See the “ Methods ” section for details. ACA autoimmune cerebellar ataxia, PEX plasma exchange
Techniques Used: Immunohistochemistry, Dot Blot, Cell Based Assay, Binding Assay, Staining, Purification, Transfection

Figure Legend Snippet: ITPR1-IgG in the CSF. Perfect overlap of the patient IgG from a CSF sample (patient 1) with a commercial antibody to ITPR1 as detected by IHC on a murine cerebellum tissue section. Bound patient IgG is depicted in green (Alexa Fluor® 488) and binding of the commercial anti-ITPR1 antibody in red (Alexa Fluor® 568); yellow indicates areas stained by both antibodies. Cell nuclei were stained by 4',6-diamidino-2-phenylindole ( blue )
Techniques Used: Immunohistochemistry, Binding Assay, Staining

Figure Legend Snippet: 3 H-thymidine uptake (cpm; mean of three stimulations) in proliferating PBMCs from patient 1 and from a healthy control following stimulation with ITPR1 or GFAP, respectively. a Note the significantly higher 3 H-thymidine uptake in patient PBMCs stimulated with ITPR1 than in the healthy donor; by contrast, no difference was noted after stimulation with GFAP or with PBS. b After subtraction of non-antigen-specific, GFAP-induced proliferation, a difference (Δcpm) between patient and healthy control of more than 600 cpm or 340 % was noted following ITPR1 stimulation
Techniques Used:

Figure Legend Snippet: ITPR1 class and subclass analysis revealed IgG1 a and IgA ( b , inset) antibodies in patient 1 and IgG2 antibodies in patient 2 (as well as in patient 3, not shown) b
Techniques Used:
3) Product Images from "Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene"
Article Title: Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene
Journal: Nucleic Acids Research
doi: 10.1093/nar/gkm608

Figure Legend Snippet: GFP expression in the brains of adult Tg( eno2:GFP ) zebrafish. ( A ) Parasagittal sections of Tg( eno2:GFP ) Pt404 (upper panel) and wild-type (lower panel) brain were labelled with an antibody to GFP. Bound antibody was detected using a histochemical reaction with a red product and nuclei were labelled with a blue counterstain. GFP expression was apparent throughout the brain of transgenic adults. ( B ) High-power view of medullary reticular formation of Tg( eno2:GFP ) Pt404 zebrafish brain, showing GFP expression in cells with neuronal morphology. The arrows show GFP-expressing neurons with axons in the plane of the section. ( C – F ) Double label confocal images of Tg( eno2:GFP ) Pt404 adult brains. Each panel consists of a set of three images: (i) Upper image; red: a cell type-specific marker of interest was localized using specific antibodies. C: Medulla; ChAT, choline acetyltransferase; cholinergic neurons; D: Olfactory bulb; TH, tyrosine hydroxylase; dopaminergic neurons; E: cerebellum; IP3R1, IP3 receptor 1; cerebellar Purkinje cells; D: thalamus; GABA, γ-amino butyric acid; inhibitory neurons; (ii) Middle image, green: GFP expression was localized in the same sections using a GFP antibody; (iii) Lower image: the merged images show co-localization of GFP and the cell type-specific marker (yellow), and were counterstained with DAPI to show nuclei (blue). The scale bar (10 µm) for each set of images is shown in the lower panel.
Techniques Used: Expressing, Transgenic Assay, Marker